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Dissertations/Thesis

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2024
Dissertations
1
  • LUANA DO NASCIMENTO SILVA BARBOSA
  • COVER CROPS UNDER PHOSPHATE SOURCES AFFECT P DYNAMICS AND SOIL MULTIFUNCTIONALITY IN SHORT-TERM CONSERVATION MANAGEMENT

  • Advisor : VALDEVAN ROSENDO DOS SANTOS
  • COMMITTEE MEMBERS :
  • ALYSSON WAGNER FERNANDES DUARTE
  • DAYANE MERCIA RIBEIRO SILVA
  • GILSON MOURA FILHO
  • RICARDO BARROS SILVA
  • VALDEVAN ROSENDO DOS SANTOS
  • Data: Feb 26, 2024


  • Show Abstract
  • Phosphorus (P) is a limiting nutrient in tropical soils, and effective phosphorus fertilization management is essential to reach critical levels for agricultural crops, despite its low efficiency in P use. The adoption of cover crops can enhance P recycling, thereby altering soil P dynamics and optimizing phosphate fertilizer efficiency. These benefits may be associated with microbial activation and soil multifunctionality. The overall objective was to determine changes in P dynamics and soil microbiota promoted by cover crops and P sources in the short term in Alagoas. The study was conducted on sandy soil over two corn crops under no-till. The treatments included two P sources (single superphosphate (SSP) and rock phosphate (RP)) and a control without P addition (Nil-P), associated with six summer cover crop species and a fallow. Chemical P fractionation, P extraction by cover crops, and corn P export were determined in both crops. Enzymes for C, N, and P-acquisition by microbiota, microbial biomass, labile C, and total soil C and N were determined only after the second crop to estimate soil multifunctionality. In the first crop, cover crops altered P dynamics, increasing ~38% compared to fallow in the labile P pool where no P was added in the 0-10 cm depth, especially with spectabilis, pigeon pea, sunn hemp, and millet. However, in the second crop, they depleted this P fraction. Where P sources were applied, the labile and moderately labile P pools increased substantially, mainly associated with millet in the 0-5 cm depth. Between crops, cover crops depleted non-labile P from the 10-20 cm and accumulated it in the initial depth (0-5 cm). Under SSP, there was an increase in P use efficiency with lab lab, spectabilis, sunn hemp, and pigeon pea compared to fallow. Similar responses occurred under RP, except with spectabilis. Cover crops modulated enzymatic activities for C, N, and P-acquisition. P-acquisition was superior under RP, especially with lab lab. Similar results with lab lab were observed for C and N-acquisition, with higher responses in the initial depth (0-5 cm), although N-acquisition was higher in Nil-P but did not differ from fallow. P addition favored microbial biomass, regardless of the depth. In No-P, similar levels occurred only in the 0-5 cm, with significant losses in the 5-10 cm. Overall, only sunn hemp and millet did not differ from fallow. Regarding soil multifunctionality, fallow was negative regardless of P sources, and the best responses occurred with jack bean and spectabilis. Thus, cover crops modulated soil P fractions due to nutrient cycling within the agricultural system (micro-soil-plant), improving phosphate fertilization efficiency and activating soil multifunctionality.

2
  • MARIA THALILLIAN SANTOS FIGUEIREDO
  • Genomic annotation of the repetitive fraction of Syagrus coronata obtained through Genome Skimming assembly

  • Advisor : ANDRE SECO MARQUES DA SILVA
  • COMMITTEE MEMBERS :
  • ANDRE SECO MARQUES DA SILVA
  • CICERO CARLOS DE SOUZA ALMEIDA
  • FLAVIA DE BARROS PRADO MOURA
  • JOSE VIEIRA SILVA
  • Data: Mar 6, 2024


  • Show Abstract
  • Plant genomes vary considerably in size and composition, with repetitive DNA being the most crucial factor in diversity, accounting for up to 90% of the genome and, of which the repetitive DNA consists of satellite and transposable elements. Syagrus coronata is an endemic palm tree from Brazil belonging to the Arecaceae family. Its geographic distribution includes the Caatinga and Atlantic Forest regions. Currently, there are studies of chloroplast and mitochondrial genomes, as well as microsatellite markers of the nuclear genome. However, the dynamics and evolution of the nuclear genome, including genome size and repetitive DNA composition, are not well understood. This study aims to estimate the genome size and characterize repetitive sequences in S. coronata. The genome size was estimated using flow cytometry and k-mer approaches. Repetitive sequences were analyzed using paired-end reads through RepeatExplorer pipelines, and the species Syagrus weddelliana and Cocos nucifera were included for genomic comparison. The genome size was estimated to be 2,27±0,026 Gbp/1C using flow cytometry and estimates using k-mer showed 1.2 Gb. The repetitive analysis showed that repetitive sequences represent 58,43 % of S. coronata genome, being the LTR retrotransposons were most abundant (48.71 %). Among the LTR retrotransposons, the Angela was the most abundate, wich 25.66 %. Other repetitive elements such as transposons, ribosomal DNA, and satellite sequences exhibited lower levels of repetition. The satellites exhibited high diversity in number and monomer length; however, they made a minor contribution to the genome size. We conclude that S. coronata has a genome with moderately repetitive DNA sequences, with LTR retrotransposons being the most abundant.

2023
Dissertations
1
  • MILENA MARIA DE FRANCA
  • ELECTROCHEMICAL, PHOTOCHEMICAL AND PHOTOELECTROCHEMICAL DEGRADATION OF SULFANILAMIDE, SULFADIAZINE AND SULFAMETHOXAZOLE USING SELF-DOPED TITANIUM NANOTUBE ELECTRODES AND DIMENSIONALLY STABLE ANODES.

     
     
  • Advisor : VINICIUS DEL COLLE
  • COMMITTEE MEMBERS :
  • VINICIUS DEL COLLE
  • JOÃO PAULO TENÓRIO DA SILVA SANTOS
  • THAISSA LUCIO SILVA
  • Data: Apr 10, 2023


  • Show Abstract
  • The vast production of chemical compounds, of natural or synthetic origin, and their multivariate applications have considerably affected the different environmental compartments and the health of humans and other animals. The world has been feeling the effects of the environmental fuel problem, which has intensified in recent years, with the diversification of industrial centers and the increase in world population. Among the AOPs, we can highlight the electrochemistry that has been used in the degradation of different pollutants and, also, the photochemistry, both approaches have been widely used in the removal of emerging pollutants. The present research performed photochemical, electrochemical and photoelectrochemical degradation of pollutants of emerging concern using dimensionally stable anodes (DSA®) and self-doped lever nanotubes (NTT-AD). DSA® electrodes were obtained commercially (DeNora do Brazil). The NTT-AD electrodes were obtained by anodizing membrane plates (Realum). All electrodes were characterized by cyclic voltammetry (CV), scanning electron microscopy (SEM) and X-ray distribution spectroscopy (EDS). The photochemical degradation of the pollutants was carried out through the application of UV radiation; the electro-oxidation was carried out through the application of different current densities (10, 15 and 20 and 30 mA cm-2) and, finally, they were degraded through the interaction of both processes (photo-electrochemistry). The efficiency of degradation pollutants was consequent by means of UV/VIS absorption spectroscopy and high-performance liquid chromatography (HPLC). The results showed that the techniques combined with each other are efficient in the degradation of the drug, however, it was also observed that UV radiation presents a satisfactory reduction.

     
     
2
  • JESSICA EMILY DOS SANTOS SILVA
  • Identification of areas susceptible to desertification in the semi-arid region of Alagoas

     
     
  • Advisor : JOSE VIEIRA SILVA
  • COMMITTEE MEMBERS :
  • JOSE VIEIRA SILVA
  • CICERO GOMES DOS SANTOS
  • FLAVIA DE BARROS PRADO MOURA
  • HUMBERTO ALVES BARBOSA
  • Data: Apr 12, 2023


  • Show Abstract
  • The semi-arid region of the state of Alagoas corresponds to approximately 32% of its territory and over the years has shown several signs of desertification. Desertification is a process derived from soil degradation and is considered one of the greatest contemporary environmental problems. Therefore, the objective was to identify, through remote sensing and field visits, desertified territories or with a high level of degradation in municipalities in the semi-arid region of Alagoas. Thus, satellite data referring to the period from 2013 to 2022 were used to identify and monitor desertified or potential regions, being processed in Qgis. In addition, photographs and notes were taken through visits to the study area. It was found that the different levels of precipitation along the time series have a significant effect on desertification rates, which showed better results for periods of higher humidity. Livestock activity is predominant in the territory analyzed, followed by the forest, which, even so, had its area reduced due to the rise of agriculture and livestock. The significant degradation of the study area and the intense anthropization of areas classified as natural in MapBiomas were evidenced through field visits, indicating the region's vulnerability to the desertification process. Remote sensing provides valuable information about the space-time dynamics of a given region, however, ensuring data integrity through in loco monitoring is essential, given the limitations to which geoprocessing tools are subject.

     
     
3
  • DYEGO HENRIQUE FERRO SILVA
  • ESTIMATE OF RAIN EROSIVITY IN THE HIGH HINTERLAND ALAGOANO THROUGH MATHEMATICAL MODELS

  • Advisor : CICERO GOMES DOS SANTOS
  • COMMITTEE MEMBERS :
  • CICERO GOMES DOS SANTOS
  • DANIELLA PEREIRA DOS SANTOS
  • MARCIO AURELIO LINS DOS SANTOS
  • RUBENS PESSOA DE BARROS
  • Data: Apr 26, 2023


  • Show Abstract
  • Erosivity is defined as the potential of rain to cause soil erosion and is a function exclusively of the physical characteristics of the rain itself, especially the quantity, intensity and diameter of drops, as well as the terminal velocity. One of the great challenges in the elaboration of models for erosion estimation consisted in transforming the kinetic energy of the rain into numerical values for application in a mathematical model for erosion prediction. The present work aims at determining rainfall erosivity based on pluviometric data in the microregions of Alagoas's Sertão do São Francisco and Serrana do Sertão de Alagoas, based on three mathematical models. The study area comprises the territorial cutout of the State of Alagoas, with municipalities distributed in the microregions Alagoana do Sertão do São Francisco (Delmiro Gouveia, Piranhas and Olho Dágua do Casado) and Serrana do Sertão Alagoano (Água Branca, Canapi and Mata Grande). In this research, pluviometric information from 06 municipalities that are part of Alto Sertão Alagoano was analyzed, in the period between 1913 and 1985, depending on the pluviometric data available for each municipality. To estimate erosivity, the models proposed by Lombardi Netto; Moldenhauer; Morais and collaboratos and Rufino and collaborators were used. Água Branca stands out within the Microregion Serrana do Alto Sertão Alagoas as the municipality that obtained the highest average erosivity values and Canapi, with the lowest values. Within the Microregion of Sertão do São Francisco, Piranhas stands out for presenting the lowest average values of erosivity and Olho Dágua do Casado with the highest average values. The average monthly precipitation for the counties that make up the Microregion of Alto Sertão Alagoas was approximately 82.81 mm for Água Branca, 51.97 mm for Mata Grande and 47.55 mm for Canapi. The lowest volume of precipitation observed was 5.50 mm in Canapi, with a historical series of 48 years of observation of rainfall data, and the highest value observed in Água Branca, with 155.85 mm, with a historical series of 72 years of observation of rainfall data. The average monthly precipitation for the municipalities that make up the Microregion of Alagoas do Sertão do São Francisco was approximately 42.49 mm for Delmiro Gouveia, 41.50 mm for Piranhas and 46.01 mm for Olho Dágua do Casado. As for the values of precipitation volume, the lowest (8.86 mm) was observed for the municipality of Olho Dágua do Casado, with a historical series of rainfall data of 28 years, and the highest (77.07 mm) registered in the municipality of Delmiro Gouveia, with 55 years of historical series of rainfall data. The highest values of average monthly erosivity were verified in the Microregion Serrana do Alto Sertão Alagoas, a region of higher altitudes, with predominantly gentle-rounded relief. The Microregion of Sertão do São Francisco, in Alagoas, presented the lowest erosivity and precipitation rates. The models presented a good correlation in all the municipalities that make up the region of the high sertão of Alagoas.

4
  • VINICIOS FERREIRA DA SILVA
  • Characterization and evaluation of the agrobiodiversity of yam in Alagoas

  • Advisor : JOÃO GOMES DA COSTA
  • COMMITTEE MEMBERS :
  • JOÃO GOMES DA COSTA
  • JACKELINE TERTO DA SILVA SANTANA
  • MAYARA ANDRADE SOUZA
  • Data: Jul 10, 2023


  • Show Abstract
  • Yam (Dioscorea spp) is a tuber-producing vegetable of paramount socioeconomic importance, being a staple food for about 300 million people. Despite its excellent nutritional and energy quality, yam crops have been suffering from several structural and genetic problems. Either because of the involvement of pathologies, or because of the loss of quality of the cultivated genotypes. Therefore, this work aimed to (i) characterize and map the agrobiodiversity of creole yam varieties in Alagoas; (ii) evaluate the varieties for the presence of foliar pathologies; (iii) quantify the phenolic and flavonoid compounds present in the extracts of three varieties. The mapping and collection of accessions were carried out from October 2022 to March 2023 using the “snowball” sampling technique and identified using a botanical key. Accessions contained in the Embrapa germplasm collection, maintained at the Research Execution Unit in Rio Largo, Alagoas, were also used. For the evaluation of leaf blight and anthracnose, 15 leaves with symptoms of the disease were collected and evaluated under a microscope and the severity computed from the diagrammatic scale, ranging from 1 to 32% for leaf blight and 0 to 100% for anthracnose. The total phenol content and the quantity of flavonoids were obtained by evaluating the varieties common face, purple yam and white yam using the Folin-Ciocalteau reagent and Aluminum Chloride. Three yam species were identified in Alagoas farmers' gardens: Dioscorea alata, Dioscorea cayennensis and Dioscorea trifida, with D. alata having white and purple pulp varieties; D. trifida with pulp in white, yellow and purple colors and D. cayennensis with pulp variations in white and cream color. For the incidence of leaf disease and anthracnose, a high incidence of both diseases was identified in the studied accessions. Finally, the common yam genotype extract had the highest phenol content (1494.5 mg EAG/g) followed by the purple yam extract (1244.3 mg EAG/g) and white yam extract (677.7 mg EAG/g). The same pattern occurred in the amount of flavonoids where the common yam extract had the highest content (43.5 mg EQ/g) followed by the purple yam extract (23.2 mg EQ/g) and the white yam extract (18. 5 mg EQ/g). According to the results obtained, it was possible to conclude that three species of yam are cultivated in Alagoas, with more than 57% of farmers using only one variety. Regarding the behavior of the accessions in relation to the foliar diseases evaluated, they showed that there was an incidence in all, but approximately 65% of them showed less incidence than the others. This result shows the importance of the diversity of genetic material to guarantee the sustainability of the culture.

5
  • MAYARA RODRIGUES NASCIMENTO
  • ESTIMATE OF RAIN EROSIVITY IN THE MUNDAÚ RIVER RIVER BASIN, IN THE ALAGOAN TERRITORY USING MATHEMATICAL MODELS

  • Advisor : CICERO GOMES DOS SANTOS
  • COMMITTEE MEMBERS :
  • CICERO GOMES DOS SANTOS
  • JOSE THALES PANTALEAO FERREIRA
  • KAMILLA ANDRADE DE OLIVEIRA
  • MARCIO AURELIO LINS DOS SANTOS
  • Data: Jul 26, 2023


  • Show Abstract
  • The knowledge of the factors that exert influence on the water erosion process has been gaining prominence that it is about management and conservation of natural resources, mainly in the hydrographic basin, which represents a territorial cut that covers different geomorphological environments. Precipitation, among the factors that act on erosion, is highlighted for adding kinetic energy to the system, which is described as rainfall erosivity, which is an exclusive function of the physical characteristics of rain, represented by the diameter of the drops, intensity and terminal velocity. One of the major challenges in the study of erosivity is to transform precipitation values into mathematical values for use in mathematical models. In this search, several mathematical models have already been developed, mainly using rainfall data, which is not the standard method for estimating rainfall erosivity. This work aims to determine the erosivity of rainfall based on rainfall data from ten municipalities in the portion of the Mundaú River Basin, in Alagoas territory, using three mathematical models. The Mundaú river basin is located in the states of Pernambuco and Alagoas, in its course it crosses different morphoclimatic domains that range from the semi-arid region to the coast. In the Alagoas part of the basin, it corresponds to its lower half, inserted in the territorial cut of fifteen municipalities in Alagoas. In this research, rainfall information was analyzed from ten municipalities that make up the Mundaú River Basin, in Alagoas territory, in the rainfall observation period from 1963 to 1985, with data from the rainfall database at the Superintendence of Northeast Development (SUDENE). To obtain rainfall erosivity values, the models proposed by Lombardi Netto were used; Moldenhauer, Rufino et al. and Rufino et al., all developed in the 90's. The model proposed by Lombardi Neto; Moldenhauer, in the nineties, in the statistical analysis presented the highest averages of erosivity in all the analyzed municipalities that encompass the Mundaú River Basin, except in São José da Laje, where the model with the highest average was that of Rufino et al. In the analysis of the relationship between precipitation and monthly erosivity, the model that presented the highest erosivity indices was the model by Morais et al. in all municipalities observed in the study. The municipality of Satuba was characterized as having the highest average annual precipitation, which consequently contributed to the highest rates of erosivity among all the analyzed municipalities. In all municipalities analyzed, the rainiest months were May, June and July, and the months with the lowest rainfall were November and December, thus presenting the lowest erosivity indices in the evaluation of the three mathematical models.

6
  • ELLEN KAROLLYNE SANTOS LOPES
  • Functional genome of Spondias tuberosa Arr. Cam. (umbu)

  • Advisor : CICERO CARLOS DE SOUZA ALMEIDA
  • COMMITTEE MEMBERS :
  • CICERO CARLOS DE SOUZA ALMEIDA
  • JOSE VIEIRA SILVA
  • MARCILIO DE SOUZA BARBOSA
  • Data: Aug 1, 2023


  • Show Abstract
  • Spondias tuberosa Arr. Cam. (umbu) is a species of tree known in northeastern Brazil for
    being an alternative and Brazilian economic resource in semi-arid regions. Thinking about
    carrying out the functional annotation of the genome of the species, the present study aimed to
    present the number of genes involved in biological processes, cellular components and
    molecular function, in addition, the codes of enzymes and metabolic pathways. For this, the
    DNA was extracted by the method of long reads using Pacbio Assembly. After detecting
    repetitive structures, using GeneMask, the structural annotation was assembled using ab initio
    gene predictors (Augustus and GeneMarker) and artificial intelligence (Helixer), for the
    qualification of genome assembly, the BUSCO tool was used. Functional annotation obtained
    data generated by the Blast2GO platform, genes were annotated using Gene Orthology (GO)
    categories, as well as enzymes and metabolic pathways. BUSCO's evaluation of the assembly
    generated by Helixer resulted in an accuracy of 98% completeness. The predicted genes were
    distributed in 51,896 total GO terms, distributed in three main categories: molecular function,
    biological process and cellular component. Enzymes were divided into 7 categories and
    metabolic pathways into 5 categories. In the present study, it was noted that the genes of the
    biological process involved in the stress response resulted in the largest number of genes for
    this category in the genome of S. tuberosa. Also revealing that the amount of genes involved
    in the response to stress is greater than the other species of the same family.

7
  • MARIA RAQUEL DA SILVA FARIAS
  • COVER CROPS AND PHOSPHATE SOURCES SOLUBLE AND REACTIVE IN THE ACCUMULATION OF MASS AND NUTRIENTS BY CORN UNDER NO-TILLAGE SYSTEM

  • Advisor : VALDEVAN ROSENDO DOS SANTOS
  • COMMITTEE MEMBERS :
  • VALDEVAN ROSENDO DOS SANTOS
  • DAYANE MERCIA RIBEIRO SILVA
  • EMERSON DOS SANTOS FERREIRA
  • RICARDO BARROS SILVA
  • Data: Aug 14, 2023


  • Show Abstract
  • The no-tillage system stands out as the predominant model used for grain production. Thus, the use of cover crops provides soil protection and nutrient fixation/cycling. Understanding the dissolution and distribution capacity of different forms of P is essential to assess the availability of this element to plants and optimize the efficient use of phosphate fertilizers, especially in regions with electropositive soil and low precipitation. This study investigated whether the use of cover crops and different P sources affect corn yield and soil quality in a no-tillage system. The experiment was carried out in the experimental area of the Federal University of Alagoas, Arapiraca campus (9°42'00" and 36°41'12"W, 324 m latitude). The local soil is classified as dystrophic red-yellow argisol and the textural class is sandy loam. The experimental design was randomized blocks, with six replications. The plots (8 x 35 m) were represented by phosphorus sources: simple superphosphate (soluble), reactive natural phosphate (reactive) and without phosphorus application (without P). In the subplots (8 x 5 m) treatments were six species of cover crops: Crotalaria juncea, Crotalaria spectabilis, Cajanus cajan, Dolichos lablab, Canavalia ensiformis, Pennisetum glaucum and one treatment without cover (fallow). The productivity of the dry mass had higher yield in Crotalaria juncea (4,1 t ha-1) and lower production for Cajanus cajan (1,1 t ha-1). The cover crops accumulated greater amounts of nutrients: N, K and Ca. There was no significant effect on corn yield in succession of cover crops and P sources. Grain yield ranged from ~6,5 to ~8 t ha-1. Corn plants extracted greater amounts of N, P and Ca. The cover crops are good nutrient recyclers and showed better extraction by the grains in the soluble source. On the other hand, the lowest nutrient extraction rates were found in fallow, except for Ca, differing only ~8% from Canavalia ensiformis. In general, cover crops contribute to soil ecosystem services, reducing temperature and water evaporation, maintaining higher humidity, and may contribute to corn productivity in regions with water scarcity, although long-term studies (>10 years) are required for positive conclusions.

8
  • VANDA DUARTE DE ANDRADE
  • APPLICATION OF HUMIC SUBSTANCES FROM WATER TREATMENT PLANT SLUDGE IN GERMINATION OF SPECIES USED IN THE RECOVERY OF DEGRADED AREAS: Chloroleucon dumosum (Benth.)

  • Advisor : WANDER GUSTAVO BOTERO
  • COMMITTEE MEMBERS :
  • WANDER GUSTAVO BOTERO
  • CICERO GOMES DOS SANTOS
  • DANIELLE GOVEIA
  • Data: Aug 14, 2023


  • Show Abstract
  • The waste called sludge, generated in Water Treatment Stations (WTP) which mostly does not have a sustainable destination, when improperly disposed of in natura in water bodies causes alarming damage. The high levels of organic matter and the ability of the sludge to interact with species present in the environment suggest applications in technological and agricultural sectors. One of the major components of natural organic matter are humic substances (SH). Derived from the oxidation and polymerization of natural organic matter and presenting various functional groups in their structure, they play a fundamental role in the environment, being responsible for transporting and making nutrients available to plants, as well as complexing metals and organic compounds, which are potentially toxic to terrestrial life. and aquatic. In view of the above, this work aimed to extract and characterize the humic substances (SH) present in sludge samples from the Water Treatment Plant in the city of Penedo, which collects water from the São Francisco River and to evaluate the potential of SH in germination of Chloroleucon dumosum seeds, a species used in the recovery of degraded areas. The study used UV-vis spectroscopy techniques, infrared spectroscopy (FTIR), analyzes for fertility purposes, organic matter content and determination of metallic species content and seed germination. The UV-vis and FTIR spectroscopy results showed that the SH present in the sample had a considerable presence of aromatic groups and carboxylic groups, characterizing the presence of hydrophilic sites in the samples. Fertility and organic matter analyzes showed that this material has great potential for use in agricultural sectors. The determination of potentially toxic metals showed a high concentration of aluminum in the sludge and SH samples, which may be highly toxic to human health and the environment, and germination analyzes showed that the concentration of 100 mg/l of SH had a positive effect .

9
  • EDILENE HONORATO DA SILVA
  • MEDICINAL MONOCOTYLEDONS NATIVE TO THE ATLANTIC FOREST: ANALYSIS OF SCIENTIFIC COLLECTIONS AND LITERATURE DATA

  • Advisor : FLAVIA DE BARROS PRADO MOURA
  • COMMITTEE MEMBERS :
  • FLAVIA DE BARROS PRADO MOURA
  • JOSE VIEIRA SILVA
  • SAMIA ANDRICIA SOUZA DA SILVA
  • Data: Aug 15, 2023


  • Show Abstract
  • Brazil is the country with the greatest biological diversity on the planet, however most of the medicinal plants known and used commercially are exotic and/or cultivated. Studies on native medicinal plants are essential for the conservation of these species and the traditional knowledge associated with their use. This study aims to systematize information available in the literature on native plants occurring in the State of Alagoas. We chose the collection records referring to the municipality of Marechal Deodoro due to its large area covered by natural ecosystems, including restingas, board forests, floodplains and lagoons. To start the study, we used the database of scientific collections available at speciesLink (https://specieslink.net/). With the initial list of monocotyledons registered for the municipality, a Google Scholar search was carried out with the term “medicinal use” for each species. There were a total of 130 species of monocotyledons in the database, distributed in 10 families. The families with the highest number of species were Cyperaceae (64), Poaceae (22), Orchidaceae (19), Araceae (11), and Xyridaceae (5). A total of 51 species (39%) occurring in the study area had at least one citation in the literature for popular medicinal use, phytochemical characterization of their extracts, in vitro activity research or in vivo activity research. These species belong to eight botanical families. There was a greater number of species mentioned in the literature in the families Poaceae, Cyperaceae, Orchidaceae, which were also the three families with the greatest diversity of species collected in the area. Species from two families that occur in the area (Alismataceae and Alstroemeriaceae) had no record of “medicinal use” with the search criteria used. The study results highlight the importance of local ecosystems as a shelter for medicinal plants and the need to plan sustainable management for some of these species that are the target of extractivism for medicinal purposes.

10
  • MARINEZ MARLENE DA SILVA
  • DEGRADAÇÃO ELETROQUÍMICA, FOTOQUÍMICA E FOTOELETROQUÍMICA DE CLORANFENICOL USANDO ELETRODOS DE NANOTUBO DE TITÂNIO AUTO DOPADO

  • Advisor : VINICIUS DEL COLLE
  • COMMITTEE MEMBERS :
  • VINICIUS DEL COLLE
  • WANDER GUSTAVO BOTERO
  • THAISSA LUCIO SILVA
  • Data: Aug 23, 2023


  • Show Abstract
  • In the current global context, emerging contaminants represent challenges in constant growth for the preservation of different environmental matrices, such as water and soil, which are essential for the preservation and maintenance of life. Such contaminants are resistant to conventional treatment processes adopted by treatment plants. In this context, the use of Advanced Oxidative Processes stands out as a promising approach for the treatment of these contaminants by providing an effective alternative in the remediation of these substances and being considered as a clean technology. [U1] In this work, the degradation of chloramphenicol, an antibiotic drug of emerging concern, was investigated using techniques such as: photochemistry, electrochemistry and photoelectrochemistry. The experiments were carried out in different radiation sources and using titanium nanotubes (NT-TiO2) as electrode. Through scanning electron microscopy (SEM) measurements, it was possible to verify that NT-TiO2 was obtained in the anatase crystalline phase. The degradation of the pollutant through the use of light, electrochemistry and photoelectrochemistry was monitored by means of UV/Vis spectrophotometry, which made it possible to monitor the variation in absorbance over time. In addition, the pollutant concentration was analyzed by high performance liquid chromatography (HPLC). It also used the infrared technique (FTIR) in order to evaluate the degradation in terms of possible intermediates through the vibrations of the observed functional groups. Finally, total organic carbon (TOC) analysis was performed to assess the degree of mineralization during the applied oxidative process. The obtained results demonstrated that a degradation rate was obtained in all AOP techniques applied, however in the photoelectrochemistry using NT-Ti electrodes and solar radiation a greater efficiency in the degradation of the contaminant under study was observed. This joint approach resulted in a significant reduction in absorbance over time, indicating the degradation of the compounds. The HPLC analysis confirmed the decrease in the concentration of the pollutant, while the FTIR analysis revealed that there was degradation of the target molecule. In addition, the TOC analysis showed a significant reduction in total organic carbon, indicating the effective removal of polluting compounds.

11
  • RENATA RIKELLY SILVA BARBOSA
  • ASSESSMENT OF SOIL QUALITY IN AN AREA UNDER THE PROCESS OF DEGRADATION IN THE MIDDLE SERTÃO ALAGOANO, USING THE TOOLS OF MULTIVARIATE STATISTICS

  • Advisor : CICERO GOMES DOS SANTOS
  • COMMITTEE MEMBERS :
  • CICERO GOMES DOS SANTOS
  • JOSE THALES PANTALEAO FERREIRA
  • MARCIO AURELIO LINS DOS SANTOS
  • RUBENS PESSOA DE BARROS
  • Data: Dec 22, 2023


  • Show Abstract
  • Currently, there is growing concern regarding the quality of environments in different parts of the world, and this concern is associated with high rates of impacts caused by human occupation. In the semi-arid environment, the effect of human actions translates into degradation, making it a significant challenge to estimate the levels of soil degradation, in terms of physical, chemical and biological attributes. Considering the dynamic and objective functionality of the soil, analyzing its current state, predicting the paths of its transformation. Measuring soil quality as an indicator of soil degradation is a widely used tool seeking to assess the degree of anthropogenic impact. Estimating soil quality in a given environment is done by observing or measuring different soil properties or processes. Despite the imminent degradation of soils in the semi-arid region of Alagoas, there are still few studies that use the physical and chemical properties of the soil as indicators of soil quality in areas undergoing degradation. In this context, this work aims to use the physical and chemical properties of the soil as indicators of the degradation process of areas undergoing desertification in the municipality of Ouro Branco, in the Alagoas hinterland. Soil samples were collected in three areas: Area 1 – Degraded; Area 2 – Intermediate and Area 3 – Native vegetation, 9 sampling points were demarcated, in two layers (0.0 – 0.10; 0.10 – 0.20 m) in the municipality of Ouro Branco – AL. The acidity and CTC properties (pH, total and effective CTC, base saturation, aluminum saturation, potential acidity, sodium saturation), macronutrients (P, K, Ca and Mg) and physical attributes (texture) were then analyzed. soil density, soil density, particle density, total porosity and aggregate stability). The use of multivariate statistics revealed the importance of certain soil attributes, standing out as sensitive for identifying or perceiving changes in soil properties, presenting values very close to degradation levels, and can be used as an indicator of physical or chemical quality of the soil. soil in studies to characterize environmental degradation.

2022
Dissertations
1
  • RICARDO ÍTALO GUIMARÃES SOUSA
  • PRODUCTION OF RADISH IRRIGATED WITH WATER TREATED IN A
    PROTECTED ENVIRONMENT IN THE AGRESTE REGION ALAGOAS

  • Advisor : CICERO GOMES DOS SANTOS
  • COMMITTEE MEMBERS :
  • CICERO GOMES DOS SANTOS
  • MARCIO AURELIO LINS DOS SANTOS
  • ANTHONY WELLINGTON ALMEIDA GOMES
  • DANIELLA PEREIRA DOS SANTOS
  • Data: Feb 8, 2022


  • Show Abstract
  • The world population grows at an exponential projection, while the production of
    food-fiber-bioenergy has been facing a series of obstacles to meet this growing
    population demand, mainly in the use of natural resources. Agriculture is one of
    the economic segments that most use the natural resource, water, with an estimate
    of around 70%. The production of agricultural on a sustainable basis involves the
    rational use of water, from the reuse of wastewater that receives treatment and
    returns to the system, mainly in irrigated agriculture. The Agreste region of the
    state of Alagoas presents one of the main limiting factors in agricultural
    production, which has been limiting the adsorption of the most competitive
    agricultural systems. The use of wastewater presents itself as a viable alternative
    from an economic, social and environmental point of view. In this context, this
    work aims to evaluate the use of wastewater from a treatment plant in the
    irrigation of the radish crop in the Agreste region of Alagoas. The experiment was
    carried out in the premises of the greenhouse at Campus Arapiraca, Federal
    University of Alagoas, from May to August 2020. The material (treated effluent)
    was obtained from the sewage treatment plant of the Alagoas State Supply
    Company (CASAL), located in the municipality of Taquarama, Agreste region of
    Alagoas. Treatments in a factorial scheme of 5 x 2 x 4, consisting of five irrigation
    depths (R1: 100% of CASAL's water; R2: 75% of CASAL's water + 25% of
    effluent; R3: 50% of CASAL's water + 50% effluent; R4: 25% CASAL water +
    75% effluent and R5: 100% effluent), two soils with different textures and four
    replications, totaling 40 sample plots. We evaluated height, fresh and dry mass of
    the aerial part; root length and fresh weight; fruit diameter and number of leaves,
    total fruit production. The microbiological evaluation of the wastewater was
    carried out by counting helminth eggs in the reuse water. Data were submitted to
    analysis of variance and means compared by Tukey test at 5%. It was found that
    they were not significant for the variables number of leaves, Brix and SPAD. The
    application of irrigation depths with dilutions of treated sewage from ETE (UASB
    Reactor + Anaerobic Filter) presented significant results for almost all variables.
    The treated effluent increased radish productivity, without compromising legal and
    sanitary aspects, as results were absent for helminth eggs.

2
  • LIDIANE MATIAS
  • ECOLOGICAL SUCCESSION IN SANDY SOIL COMMUNITIES

  • Advisor : FLAVIA DE BARROS PRADO MOURA
  • COMMITTEE MEMBERS :
  • FLAVIA DE BARROS PRADO MOURA
  • CICERO CARLOS DE SOUZA ALMEIDA
  • JOSE VIEIRA SILVA
  • RAFAEL RICARDO VASCONCELOS DA SILVA
  • NADJACLEIA VILAR ALMEIDA
  • Data: Jun 13, 2022


  • Show Abstract
  • In this research we discuss the centrifugal spatial pattern in the establishment of vegetation in sandy coastal soils, according to a model in which a plant acts as a catalyst for the succession process and triggers the process of clump formation. We studied vegetation growth in a conservation unit in the State of Alagoas (APA da Marituba), analyzing 100 thickets and their growth at two different times, with an interval of 10 years. The intensity of the spatial pattern was quantified using landscape metrics. Our data indicate that a key plant (Myrciaria floribunda) initiates a process creating regeneration nuclei. The initial colonization process appears to follow an assembly line, with a deterministic succession pattern until the thickets reach a size of 10m. In the successional sequence, nuclei grow and neighboring nuclei coalesce. The growth of the nuclei occurred both by the arrival of propagules of new species and by the addition of new individuals of species from the initial assembly. It was observed that the thickets of smaller areas tended to present rounded shapes and as the thickets increase in area, their shape becomes linear and the index values tend to increase. The taxonomic richness of clumps, at species level, increases along the clump size gradient Richness was also correlated with clump shape, larger and irregular clumps were richer in species.

3
  • RUBIANA DO NASCIMENTO LIRA
  • ESTIMATION OF RAIN EROSIVITY IN THE CORURIPE RIVER BASIN THROUGH MATHEMATICAL MODELS

  • Advisor : CICERO GOMES DOS SANTOS
  • COMMITTEE MEMBERS :
  • CICERO GOMES DOS SANTOS
  • MARCIO AURELIO LINS DOS SANTOS
  • DANIELLA PEREIRA DOS SANTOS
  • RUBENS PESSOA DE BARROS
  • Data: Jun 28, 2022


  • Show Abstract
  • The potential of rain to cause erosion can be assessed through erosivity indices. Rainfall erosivity, defined as the potential for rain to cause erosion, depending exclusively on the physical characteristics of the rain itself, including intensity, quantity, raindrop diameter, terminal velocity and kinetic energy. The erosivity estimate is obtained using a series of rainfall records of at least 20 years. The pluviographic method is the standard method used in Brazil. But due to the deficiencies of rainfall records, seeking to overcome this deficiency was proposed by Lombardi Neto, a mathematical model, which establishes a relationship between the values of monthly rainfall for an annual period, the result is called rainfall coefficient. The Coruripe River basin extends for more than 140 km, from its source on the edge of the Borborema Plateau, in the municipality of Palmeira dos Índios and flows into the Atlantic Ocean, in the municipality of Coruripe. This route crosses different regions, with many in the pluviometric regimes, which has an influence on the erosivity indices in the territorial cut of the aforementioned basin. Based on this assumption, this work aims to determine the rainfall erosivity indices of the municipalities that are part of the Coruripe River basin - AL, through mathematical models. In this research, pluviometric information from 12 municipalities that are part of the Coruripe River Watershed, in the period studied is between 1913 and 2021, depending on the pluviometric data available for each municipality. To estimate erosivity, the models proposed by Leprun and Lombardi Netto were used; Moldenhauer. The municipality of Quebrangulo presented the highest erosivity indices, according to the models proposed by Leprun and Lombardi Neto; Moldenhauer. The municipality of Craíbas had the lowest erosivity indices by the models studied, perhaps due to the amplitude of the rainfall data. The highest values of monthly average erosivity were verified in the region of Alto Coruripe, region where the east is located, with a very busy relief. The region called middle Coruripe, presented the lowest erosivity and precipitation indexes, this result is conditioned to the values presented by the municipality of Craíbas. The models showed a good correlation in all the municipalities of the river basin of the river Coruripe - AL.

4
  • RUBIANA DO NASCIMENTO LIRA
  • ESTIMATION OF RAIN EROSIVITY IN THE CORURIPE RIVER BASIN THROUGH MATHEMATICAL MODELS

  • Advisor : CICERO GOMES DOS SANTOS
  • COMMITTEE MEMBERS :
  • CICERO GOMES DOS SANTOS
  • DANIELLA PEREIRA DOS SANTOS
  • MARCIO AURELIO LINS DOS SANTOS
  • RUBENS PESSOA DE BARROS
  • Data: Jun 28, 2022


  • Show Abstract
  • The potential of rain to cause erosion can be assessed through erosivity indices. Rainfall erosivity, defined as the potential for rain to cause erosion, depending exclusively on the physical characteristics of the rain itself, including intensity, quantity, raindrop diameter, terminal velocity and kinetic energy. The erosivity estimate is obtained using a series of rainfall records of at least 20 years. The pluviographic method is the standard method used in Brazil. But due to the deficiencies of rainfall records, seeking to overcome this deficiency was proposed by Lombardi Neto, a mathematical model, which establishes a relationship between the values of monthly rainfall for an annual period, the result is called rainfall coefficient. The Coruripe River basin extends for more than 140 km, from its source on the edge of the Borborema Plateau, in the municipality of Palmeira dos Índios and flows into the Atlantic Ocean, in the municipality of Coruripe. This route crosses different regions, with many in the pluviometric regimes, which has an influence on the erosivity indices in the territorial cut of the aforementioned basin. Based on this assumption, this work aims to determine the rainfall erosivity indices of the municipalities that are part of the Coruripe River basin - AL, through mathematical models. In this research, pluviometric information from 12 municipalities that are part of the Coruripe River Watershed, in the period studied is between 1913 and 2021, depending on the pluviometric data available for each municipality. To estimate erosivity, the models proposed by Leprun and Lombardi Netto were used; Moldenhauer. The municipality of Quebrangulo presented the highest erosivity indices, according to the models proposed by Leprun and Lombardi Neto; Moldenhauer. The municipality of Craíbas had the lowest erosivity indices by the models studied, perhaps due to the amplitude of the rainfall data. The highest values of monthly average erosivity were verified in the region of Alto Coruripe, region where the east is located, with a very busy relief. The region called middle Coruripe, presented the lowest erosivity and precipitation indexes, this result is conditioned to the values presented by the municipality of Craíbas. The models showed a good correlation in all the municipalities of the river basin of the river Coruripe - AL.

5
  • ANA CLEIA BARBOSA DE LIRA
  • Effect of essential oils on post-harvest treatment of tomato (Solanum lycopersicum L.) in the Agreste region of Alagoas.

     

  • Advisor : JOÃO GOMES DA COSTA
  • COMMITTEE MEMBERS :
  • JOÃO GOMES DA COSTA
  • JHONATAN DAVID SANTOS DAS NEVES
  • RUBENS PESSOA DE BARROS
  • Data: Jun 29, 2022


  • Show Abstract
  • The tomato fruit (Solanum lycopersicum L.) is versatile, being consumed fresh or processed, but has a limited shelf life. Therefore, the use of natural substances with antimicrobial and antifungal properties are important for use as post-harvest treatment. Thus, the objective was to evaluate the effects of copaiba and propolis essential oils on post-harvest characteristics and physicochemical parameters in tomato fruit variety TY-16. The fruits were immersed in the prepared solutions of essential oils with the exception of the control treatment, in which they were only washed with the chlorinated solution. A completely randomized design and factorial scheme (2 x 5) were used. Data were determined at 0, 5, 10 and 15 days and subjected to analysis of variance by the F Test and the use of Dunnett's test to compare each concentration versus control, at a 5% probability level (p<0.05) . Data were also subjected to regression analysis to determine the best dose. The statistical program used was GENES. There was no significant difference between the concentration levels of green propolis and, for red propolis, there was a significant difference only for Brix, highlighting the treatments with doses of 2 and 4%. The most interesting effects were those obtained with the treatments of fruits with copaiba. The distilled copaiba presented better results at 5% and 7% of inclusion and the balsamic copaiba as the inclusion level increases, the mass loss and fruit firmness decreases. The estimate of the most efficient doses for post-harvest treatment, from the regression equations, would be 24.45% for the distilled extract of copaiba and 20.98% for the balsamic extract of copaiba.

6
  • MARIA RENATA MARIANO DA SILVA
  • IDENTIFICATION OF CAATINGA DEGRADED AREAS IN THE CAPIÁ RIVER HYDROGRAPHIC BASIN, SEMIARIDO OF BRAZIL.

  • Advisor : JOSE VIEIRA SILVA
  • COMMITTEE MEMBERS :
  • FLAVIA DE BARROS PRADO MOURA
  • JOSE VIEIRA SILVA
  • PEDRO LUCAS COSMO DE BRITO
  • Data: Jun 29, 2022


  • Show Abstract
  • The Caatinga biome is the main set of terrestrial ecosystems in the Brazilian Northeast region. The Caatinga vegetation domain is diversified in landscapes and vegetation types; however, it is advancing in a stage of degradation with few conserved remnants. To understand the dynamics of such a diverse environment, it is important to consider watersheds as units. In this way, we sought to identify degraded areas in the Capiá River Basin, during the period from 2001 to 2021, inserted in the Caatinga Biome, Brazilian semi-arid region. For this, remote sensing products were used, where, through SRTM, LANDSAT 5/TM and 8/OLI images , data from MapBiomas Brazil and CHIRPS ; used in Google Earth Engine and Quantum gis ( Qgis ) allowed to evaluate the slope, Vegetation Indexes - IVs , land use and cover and precipitation. In addition, 10 targets were selected on the ground of the watershed, where data collection was carried out in the field. The study showed that most of the region has very low and low slopes (0-3% and 3-6%); the IVs used showed similar results with greater expression of vegetation cover for the last years of the time series, the spatial variation of vegetation is mainly related to slope and precipitation. With regard to land use and occupation, for the last decade, reductions were registered in the area (%) of the class’s natural forest, non-forest formation and water bodies, in contrast, an increase in agriculture and livestock and urban areas. The on-site visit represented a fundamental step for verifying the remote data, with images of each point being recorded. The data show that environmental monitoring studies are necessary to understand the dynamics of changes over time, the use of remote sensing offers many options as study tools, however, when validated in the field, they provide greater integrity.

7
  • ALEX TEÓFILO DA SILVA
  • SOLUBILIZATION OF INORGANIC PHOSPHATE BY CAATINGA BACTERIA AND FUNGI

  • Advisor : ALYSSON WAGNER FERNANDES DUARTE
  • COMMITTEE MEMBERS :
  • ALYSSON WAGNER FERNANDES DUARTE
  • MICHEL RODRIGO ZAMBRANO PASSARINI
  • RUBENS PESSOA DE BARROS
  • Data: Jun 29, 2022


  • Show Abstract
  • The Caatinga, unique biome in Brazil, has unique climatic characteristics, marked by high temperatures and low rainfall. The microorganisms of the Caatinga produce biologicals that are little explored and that can be strategic for agricultural use, such as phosphate solubilization. From this perspective, the objective was to evaluate the solubilization of inorganic phosphate by bacteria and fungi isolated from lichens from the Caatinga of Alagoas, as well as to quantify the solubilization under different stress conditions. The samples of crusty and fruity lichens were collected in the semiarid from Alagoas, in the municipality of Santana do Ipanema-AL, in an environmental protection region, the Tocaia reserve, and in an impacted region, the Serra do Gugi. A total of 129 fungi and 152 bacteria were evaluated, 08 of which were positive for inorganic phosphate solubilization at 25.0 °C. The strains were selected for the other tests and evaluated at different temperatures at 25.0, 30.0 and 35.0°C in solid medium and evaluated the solubilization index (IS), were: 1. LCB2, 5. LCB3, 6. LCB3, 7. LCB3, 8. LCB3, 15. LCB3, 9. LCB7, and 6. UVLCB5. It was observed that the bacteria were able to solubilize in the three temperature ranges. The isolates that had the highest (IS) were: 9. LCB7 (3.42); 1. LCB2 (4.83); 15. LCB3 (8.27) at 25.0°C, while at 30.0°C the isolate with the highest IS was 7. LCB3 (4.31); 1. LCB2 (5.33) and 15. LCB3 (12.05), at 35.0°C strain 15. LCB3 maintained the highest IS with (10.72) strain 1. LCB2 (4.58). Overall the halos ranged from 2.0 mm to 31 mm respectively. In the solubilization tests with the fungal isolates in NBRIP solid medium, there were no positive results. In liquid medium, 02 isolates 1. LCB2 and 9. LCB7 showed significant values for P quantification at 35.0°C the bacterium 9. LCB7 obtained an average release of soluble P of 520.53 mg/L, with pH reduction to 4.11 and at 30.0°C, the 1. LCB2 had an average release of soluble P of 563.73 mg/L, with a pH reduction to 4.14. Assays at different pH (5.0; 7.0 and 9.0) strain 1. LCB2 provided better solubilization at pH 9.0 with an average of 551.90 mg/L of soluble P and pH reduction to 3, 69 conferring better adaptability to the alkaline environment, the same occurred with strain 9. LCB7 that obtained greater solubilization at pH 9.0 with an average of 455.88 mg/L and reduction of pH 3.75; Isolate 9. LCB7 had the best solubilization average at pH 9.0 with an average release of soluble P of 455.88 mg/L with pH reduction to 4.75; the same occurred with isolate 1. LCB2, which obtained an average P release of 551.90 mg/L at pH 9.0 with a decrease in pH to 3.69. In tests with different concentrations of NaCl, both strains solubilized better up to 0.5 M, being 607.7 mg/L for strain 9. LCB7, strain 1. LCB2 conferred 236.34 mg/L of soluble P at 1.0 M. Regarding the different carbon sources tested, both strains maintained glucose as the best carbohydrate source with an average of 558.59 and 532.53 mg/L respectively. In contrast, for different nitrogen sources, strain 1. LCB2 conferred a higher level of soluble P with Urea 479.74 mg/L, strain 9. LCB7 had ammonium sulfate with the highest solubilization of 617.75 mg/L. In the kinetics, solubilization occurred exponentially for both bacteria, reaching 683.1 mg/L on the 7th day (144h) of incubation for 1. LCB2 and 667.82 mg/L for 9. LCB7. In the HPLC analysis, lactic, citric, succinic and fumaric acids were present, while 9. LCB7 only fumaric acid was not present in the 144 h of the assay, strain 1. LCB2 produced only citric acid until the 72 h period and produced the fumaric acid until the end of the experiment. The tested microorganisms isolated from Caatinga lichens have great potential for solubilizing inorganic phosphates, as well as being able to resist temperature levels, bringing new perspectives for field tests, with the possibility of a new inoculant for agricultural crops.

8
  • MARCELO CAPISTRANO COSTA
  • Study of Phenol Degradation by Electrochemical and Photoelectrochemical Techniques

  • Advisor : VINICIUS DEL COLLE
  • COMMITTEE MEMBERS :
  • JOÃO PAULO TENÓRIO DA SILVA SANTOS
  • THAISSA LUCIO SILVA
  • VINICIUS DEL COLLE
  • Data: Jun 29, 2022


  • Show Abstract
  • Phenol is an aromatic compound of industrial importance, with its structural properties being able to be used in a variety of products, such as medicines and agrochemicals. As it is an environmental contaminant solution that is difficult to remove by conventional methods of water treatment, its presence in a combination of its treatment alternatives for new technologies for reduction. The present study aimed to carry out the work of phenol degradation through electrochemical and photochemical tests by ultraviolet (UV) irradiation and advanced oxidative processes (AOPs). The electrochemistry, advanced photoelectrochemical photochemistry, study evaluated the manufacturing of oxidative processes employing H2O2 as an oxidizing agent. The tests were carried out in a glass reactor equipped with a 13 W UV lamp with radiation emission at 254 nm used in the photochemical tests. In the electrochemical evaluation, three current densities were followed (10, 20, and 30 mA cm-2) using Na2SO4 as an electrolyte, in which a density of 10 mA cm-2 was chosen for the electrochemical evaluation. Phenol monitoring was monitored in a spectrophotometer with spectral scanning between 250 and 800 nm and by LC. Through the analysis of time analysis, comparisons of degradation products were made in times: 60 minutes, 120, and 120 minutes. The reduction of phenol was verified in all estimates, but it was not significant in the tests, in addition to presenting a reduction of its electrochemical concentration in the reaction medium, using the oxidant H2O2. In the photochemical, photochemical, and photochemical tests performed, there was a reduction of phenol, with greater efficiency with the reduction of tests performed, with H2O2. Appreciable also of these nodes with UV and H2O2 irradiation. Thus, the tests with photochemical and electrochemical components combined with AOPs proved to be efficient in the decomposition of phenol and degradation products.

9
  • MARIA GLEIDE JANE LIMA DE GÓIS
  • Cover crops and sources of soluble and reactive phosphorus in corn production in non-till systems

  • Advisor : VALDEVAN ROSENDO DOS SANTOS
  • COMMITTEE MEMBERS :
  • MARCILIO DE SOUZA BARBOSA
  • RICARDO BARROS SILVA
  • VALDEVAN ROSENDO DOS SANTOS
  • Data: Jun 29, 2022


  • Show Abstract
  • In the grain producing regions of Alagoas, Yellow Ultisols and Latosols predominate, with medium texture, acidic, with high saturation by exchangeable aluminum; low levels of organic matter; low cation exchange capacity and deficient in phosphorus (P) available to be absorbed by plants due to the adsorption of this element with clays and iron and/or aluminum oxides in the soil. To achieve high levels of productivity in these soils, it is necessary to apply high doses of nutrients, particularly P. The inclusion of cover crops can contribute to increase the availability of P in the soil and improve the efficiency of use of phosphate fertilizers. A field experiment was carried out in the experimental area of UFAL Campus Arapiraca, with the objective of evaluating the effects of cover crops and phosphorus sources on yield, production components and nutrient extraction by corn in a no-till system. The dry mass of the aerial part of the cover crops ranged from 1.3 to 4.3 t ha-1. Sunn hemp produced more dry mass than the other species. Cover crops accumulated higher amounts of nitrogen, potassium and calcium and lower amounts of phosphorus, magnesium and sulfur. Corn grain yield ranged from 7.7 to 8.3 t ha-1 and was not influenced by P sources and cover crops. Nutrient extraction by corn grain and shoots was influenced by coverage and sources, with a tendency of larger amounts for the soluble source.

2021
Dissertations
1
  • THAIS RAYANE GOMES DA SILVA
  • WATER CONSUMPTION OF PULSES IRRIGATED PEPPER IN DIFFERENT TIMES

  • Advisor : MARCIO AURELIO LINS DOS SANTOS
  • COMMITTEE MEMBERS :
  • CICERO GOMES DOS SANTOS
  • GERÔNIMO FERREIRA DA SILVA
  • MARCIO AURELIO LINS DOS SANTOS
  • MARCOS ALEX DOS SANTOS
  • Data: Mar 16, 2021


  • Show Abstract
  • Pepper (Capsicum annuum L.) is among the ten vegetables of greatest economic importance in the national market for vegetables, and to obtain high productivity it is necessary to use appropriate techniques in irrigation management. In light of this, the irrigation pulses consist of applying the necessary real irrigation in a fractional way throughout the day. Thus, the objective was to determine the crop evapotranspiration (ETc) and the cultivation coefficient (Kc) through drainage lysimeters with the inclusion of irrigation pulses at different times of cultivation in the pepper culture. The experiments were carried out in the experimental area for the Irriga Group, located on the premises of the Federal University of Alagoas, Arapiraca Campus, in the periods from January to March, May to July and August to October 2019. 25 drainage lysimeters were installed, distributed in five planting lines, in an area of 6.0 x 3.0 m in width and length, respectively, consisting of plastic material with a volume of 0.06 m3. Each set of 5 lysimeters corresponded to an irrigation pulse, the first set being called pulse 1 to set 5 which was called pulse 5. The Kc was obtained for each irrigation pulse, being determined by the ratio between ETc and the ETo (reference evapotranspiration). ETc was obtained daily for each pulse of irrigation, which was determined by the difference between the applied and drained blade. And ETo estimated through empirical methods: Penman-Monteith, Hargreaves-Samani, Solar Radiation and Blaney-Criddle. The first growing season showed the respective Kc's: pulse 1 with the lowest Kc (0.61; 0.61; 0.81; 1.09 and 1.24) and pulse 5 with the highest Kc (0.77; 0, 77; 0.89; 1.15 and 1.27), the 2nd growing season follows the same pattern of behavior with the lowest Kc for pulse 1 (0.55; 0.55; 0.74; 0.94 and 1.16) and the highest Kc at pulse 5 (0.62; 0.62; 0.76; 1.14 and 1.29), which continues to happen in the third growing season, where pulse 1 obtained lowest Kc (0.59; 0.59; 0.84; 1.04 and 1.22) and pulse 5 the highest Kc (0.70; 0.70; 1.00; 1.26 and 1.30 ) when compared to the others. In all growing seasons, ETc increased with the addition of irrigation pulses, in the 1st growing season, pulse 1 had a lower ETc with 8.36 mm (Total: 307.58 mm) and pulse 5 had a higher ETc with 8.40 mm (Total: 336.16 mm); in the second growing season, pulse 1 presented 4.41 mm ETc (Total: 171.74 mm) and pulse 5 of 4.51 mm (Total: 187.62 mm); and the third cultivation season, obtained for the wrist 1 an ETc of 8.92 mm (Total: 335.14 mm) and for the wrist 5 an ETc of 9.50 mm (Total: 386.35 mm). With respect to Kc, pulse 1 obtained the lowest and pulse 5 the highest, when compared to the others.

2
  • FRANCISMARY BARROS DA SILVA
  • SEQUENCING OF CHLOROPLAST GENOMES AND PHYLOGENETIC ANALYSIS IN THE GENUS Spondias (Anacardeaceae)

  • Advisor : CICERO CARLOS DE SOUZA ALMEIDA
  • COMMITTEE MEMBERS :
  • CICERO CARLOS DE SOUZA ALMEIDA
  • FLAVIA DE BARROS PRADO MOURA
  • JOSE VIEIRA SILVA
  • Data: Apr 14, 2021


  • Show Abstract
  • The plastome have a uniparental origin (usually maternal), while rDNA have a biparental origin, allowing to obtain robust phylogenies. The objective of this study was to assemble the chloroplast genomes of Spondias sp (umbuguela), (S. purpurea) and (S. dulcis), to build a robust phylogeny for the genus Spondias through the combination of SNP analysis, nrDNA and chloroplast alleles, using data from new generation sequencing. DNA extraction was performed using the CTAB method. The sequencing of the reads was carried out at GenOne Soluções em Biotecnologia, Rio de Janeiro, Brazil, where 5 five mg of gDNA was used for each sample to build the library. Sequencing was generated using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, UK). The library was analyzed for fragment distribution using Agilent2100 Bioanalyzer and quantification by PCR in real time. The library was of the pairedend type (2 x 150 pb) sequenced with Illumina NovaSeq 6000 sequencer. Genome assembly and SNP analysis were carried out at the Genetic Resources Laboratory at UFAL, using the (S. tuberosa) genome as and by reference the Genes annotation tool. All notes were checked manually. It was used and the complete genome was represented using the software OGDRAW (Draw Organelle Genome Maps) to represent the complete genome. For SNP analysis, the readings were mapped in the reference using the Bowtie2 software and the result due in graphical HeatMap. The species plastidial genomes of the species of the genus Spondias analyzed in the present study proved to be highly conserved, with size Spondias sp (umbuguela) (162,577 bp), (S. purpurea) (162,728 bp), and (S. dulcis) (162,245 bp) with a total of 137 genes for Spondias sp (umbuguela) and (S. dulcis), including 37 tRNAs and 8 rRNAs. While (S. purpurea) presented 136 genes, including 37 tRNAs and 8 eight rRNAs. Spondias purpurea shared a greater number of SNPs with Spondias sp (umbuguela) and are being closely related to and grouped in the same clade, suggesting what suggests that umbuguela is not a hybrid.

3
  • MARÍLIA LAYSE ALVES DA COSTA
  • Characterization of humic substances from Oxisols distributed in the

    state of Alagoas

  • Advisor : WANDER GUSTAVO BOTERO
  • COMMITTEE MEMBERS :
  • CICERO GOMES DOS SANTOS
  • OSEAS SILVA SANTOS
  • WANDER GUSTAVO BOTERO
  • Data: Jul 22, 2021


  • Show Abstract
  • The semi-arid region of Northeastern Brazil (NEB) has high soil
    variability, highlighting the State of Alagoas, which has a strong
    diversity of climate, relief and soils typically belonging to the Oxisols
    and Argissolos classes. Studies related to Oxisols present in the state of
    Alagoas are still incipient, which makes it difficult to know the
    physical-chemical properties, as well as to provide better use of such
    soils, in order to avoid processes of environmental degradation, such as
    desertification. The present work aimed to extract and characterize the
    organic matter present in Yellow, Red and Red-Yellow Latosols
    frequently found in different regions of the state of Alagoas, seeking to
    establish data that can help in decision making and improvement of
    agriculture in areas subject to desertification. The samples of Oxisols
    were collected between the months of October and November of 2018,
    in the cities of Alagoas (Teotônio Vilela, Arapiraca, Coruripe, Palmeira
    dos Índios, Limoeiro de Anadia and Rio Largo). Fertility analysis of
    the eight samples of Oxisols consisted of determining the pH in water
    and calcium chloride, Calcium, Magnesium and Aluminum Method
    Exchangeable Calcium, Exchangeable Aluminum, Exchangeable
    Sodium and Potassium, and Potential Acidity The organic matter of the
    soil samples was determined by the calcination method, and the
    extraction of HS occurred by the method adopted by the International
    Society of Humic Substances (IHSS). The characterization of the SH
    samples was performed both by ultraviolet spectroscopy in the visible
    region and by molecular fluorescence spectroscopy. Micro, 

    macronutrients and metallic species were determined by the open
    system digestion method. Through the analysis of fertility, it was found
    that these are strongly acidic soils, highlighting soil H, due to the high
    content of expressed potential acidity. The MOS content was
    expressive in all samples, highlighting the E (9.23±0.49) and D
    (8.02±0.08) soils, which presented a higher mean MOS content when
    compared to the other samples, which directly influenced the increase
    in the cation exchange capacity. According to the E4/E6 ratio, sample
    B has a greater presence of condensed aromatic structures (3.98), while
    sample C has a greater presence of aliphatic structures (24.88), low
    molecular weight (11.50) and with greater participation of terrestrial
    plants, due to the high level of lignified structures. While the
    fluorescence characterization through the A4/A1 ratio, it was
    evidenced that the F sample has a higher humification index, where
    consequently there was a change in MOS in SH, expressing itself as a
    soil of undesirable quality. Sample E expressed a higher average
    content of the elements evaluated (Ba, Co, Cr, Cu, K, Mo, Ni, Pb, Sr,
    Zn, Al and Fe). Therefore, the present research becomes relevant to
    obtain a more accurate knowledge of this soil class in the state of
    Alagoas, as well as scientifically contributing so that these are
    conditioned to maintenance in order to maintain their fertility.

4
  • ADIELMA MARIA DE MENEZES
  • POSTHARVEST QUALITY CHARACTERIZATION OF ACCESSES OF DIOSCOREA SPP. COLLECTED IN ALAGOAS AND SERGIPE

  • Advisor : JOÃO GOMES DA COSTA
  • COMMITTEE MEMBERS :
  • JOÃO GOMES DA COSTA
  • JOAO PAIXAO DOS SANTOS NETO
  • MAYARA ANDRADE SOUZA
  • Data: Sep 6, 2021


  • Show Abstract
  • The aim of the study was to characterize accessions of yam Dioscorea spp. as to the proximate composition (moisture, ash, lipids, carbohydrates and proteins), the total energy value (TEV) and the content of mineral elements. The accesses were obtained from collection expeditions in the main producing municipalities in the States of Alagoas and Sergipe. Thus, two accessions of Dioscorea trifida were captured; six from D. cayenensis, one from D. bulbifera and one. D. alata. All analyzes were carried out from 11/23/2020 to 12/10/2020. The dataset was submitted to the Kolmogorov-Smirnov normality test and, as it presented normality, descriptive statistics such as mean and standard deviation and multivariate analyzes were analyzed as a determination of genetic divergence from dissimilarity measures such as the mean Euclidean distance and cluster analysis as the Tocher method. The results sought that the accessions of different species of Dioscorea have potential for use in human food and that they can be better used by local populations. As, for example, we can mention the accessions 2, 9 and 10 which have protein content above the average in relation to the other genetic materials. As well as some accessions that stood out, such as Access 10 that presented high levels of zinc, magnesium and potassium, while Access 5 stood out in relation to manganese content. On the other hand, the study showed the little variability that exists in the materials, evidencing the need to leave these materials and rescue those that the producers of the different traditional categories established to plant.

5
  • WANESKA DOS SANTOS
  • Response of Carpophilus spp. to the odors of the pine tree blossom and the fruits of pine cones and guava in an advanced stage of maturation

  • Advisor : JOÃO GOMES DA COSTA
  • COMMITTEE MEMBERS :
  • CENIRA MONTEIRO DE CARVALHO
  • EDJANE VIEIRA PIRES
  • JOÃO GOMES DA COSTA
  • Data: Sep 8, 2021


  • Show Abstract
  • Among the species belonging to the family of annonaceae, the pine cone (Annona squamosa L.), is one of the representatives of the family of greatest socioeconomic importance worldwide. In Brazil, its largest production is concentrated in the Northeast region, with family farming being responsible for all the pine cones produced in the country. However, the production of fruits of this species is below its potential due, among other factors, to deficient pollination, since normally the presence of its pollinators is insufficient to reach satisfactory productions. Artificial pollination has good results, but it is costly. Thus, the objective of this work was to use volatile organic compounds (odors) that are attractive to Carpophilus spp. both in the laboratory and in the field. The volatile organic compounds (VOCs) of the pine cone and guava fruits, as well as the VOCs emitted by the pine tree flowers, were collected using the dynamic headspace system, using Porapak Q adsorbent (80/100 mesh, 0.05 g; Supelco). The air was injected into the plastic bag, passing through an activated carbon filter at a flow of 400 mL/min. for both fruits and flowers and tubes with Porapak Q were used at the top of the bag and the air was collected at a flow rate of 350 mL/min. for each fruit/flower. The collection of VOCs from the pine cone flowers lasted twelve hours starting at 5:00 pm, as this time was marked by the release of a strong sweet odor, which remains until the next day. Previously, the glass tubes were washed with hexane and heated in ovens at 100 °C for 60 min, for the "activation" of the adsorbent. At the end of the aeration, the desorption of volatiles in Porapak Q was carried out passing 0.5 mL of hexane solvent and stored in vials at -20 °C. As verified, the volatiles of the guava fruit, the pine cone flower and the pine cone fruit showed that they are attractive to the pine tree pollinators, both in the laboratory and in the field. The VOCs of the guava fruit and the pine cone in advanced stages of maturation attracted the pine pollinating insect, as well as the flower VOCs. That's because a floral odor similar to ripe or decaying fruit can attract nidullid beetles. Since, these insects are attracted by floral scents that have a “fruitful” aroma. The flowers probably mimic fruit aromas in order to attract these insects. The use of attractive baits in pine cone orchards, aiming to increase productivity, showed satisfactory results, increasing productivity in commercial orchards by more than 80%.

6
  • MILLENA DE ARAUJO RODRIGUES
  • PEANUT IRRIGATED WITH DIFFERENT WATER BLADES AND DISTINCTS DOSES OF PHOSPHATE AND ORGANIC FERTILIZATION

  • Advisor : MARCIO AURELIO LINS DOS SANTOS
  • COMMITTEE MEMBERS :
  • MARCIO AURELIO LINS DOS SANTOS
  • CICERO GOMES DOS SANTOS
  • GERÔNIMO FERREIRA DA SILVA
  • MARCELO CAVALCANTE
  • Data: Sep 9, 2021


  • Show Abstract
  • Peanuts are the fourth most cultivated oilseed in the world, but in order to obtain high yields in the Semiarid region, irrigation is necessary. The production of this crop is related to soil fertility, and phosphorus is considered the main factor of crop productivity. It is also widely used as a fertilizer for chicken litter, as it presents the main nutrients (nitrogen, phosphorus and potassium). Thus, the objective was to evaluate the response of peanut crops under different irrigation depths and different doses of phosphate and organic fertilization. The experiment was conducted in the experimental area of the IRRIGA Group, at the Federal University of Alagoas, Campus de Arapiraca, with a randomized block design in a 5 x 5 x 4 factorial. The treatments applied consisted of 5 irrigation depths: 50, 75, 100, 125 and 150% of daily crop evapotranspiration (ETc), 5 doses of phosphate fertilization: 0, 50, 100, 150 and 200% of the crop recommendation, and in the other experiment, five levels of fertilization (2, 4, 6, 8 and 10 t/ha), with 4 repetitions. Each experimental plot was composed of 27 plants, distributed in 1.0 m², evaluated only the 10 central plants. The ETc was determined using the SLIMCAP system, used to apply water depths. The variables studied were: plant height (AP), number of pods (NVV) and number of stalks (NHP) per plant, average length (CMV) and average diameter (DMV) of the pod, volume of pod per plot (VVP) , green weight (PVV) and dry weight (PSV) of the pod, and relative chlorophyll index (SPAD). The results concluded were that for phosphate fertilization the variables SPAD, NVP, VVP and PVV respond to the interaction of irrigation depth and doses of phosphate fertilization. AP, NH and PS respond to lamina and phosphate fertilization alone. On the other hand, CM responds significantly only to phosphate fertilization and DM is not influenced by the factors studied. The maximum production points of most variables were obtained with the irrigation depth higher than ETc and fertilization (P2O5) lower than recommended for the crop. The results for the experiment with chicken litter showed that the irrigation depths positively influenced the variables: AP, NHP, NVP, DMV, VVP, PVV, PSV, in which they obtained better performance at 100% ETc; the variables CMV and chlorophyll index (SPAD) had a decreasing influence with the increase of irrigation depths, the greater the irrigation depth, the lower the value obtained; the doses of organic fertilizer had no positive influence, only on the chlorophyll index (SPAD), obtaining higher values in the doses closer to 100% of the recommendation.

7
  • ADRIELY VITAL DE SOUZA SILVA
  • Solubilization of inorganic phosphate by filamentous fungi isolated from lichens and sediment from Antarctica

  • Advisor : ALYSSON WAGNER FERNANDES DUARTE
  • COMMITTEE MEMBERS :
  • ALYSSON WAGNER FERNANDES DUARTE
  • FLAVIA DE BARROS PRADO MOURA
  • MICHEL RODRIGO ZAMBRANO PASSARINI
  • VALDEVAN ROSENDO DOS SANTOS
  • Data: Sep 9, 2021


  • Show Abstract
  • Antarctic shelter environments can different life forms adapted to physical conditions and extreme areas such as cold temperature, water availability, high UV radiation, strong winds, different pH and salinity ranges. Given this information, it is suspected that filamentous fungi adapted to such conditions develop the activity of solubilizing insoluble phosphate from the soil to release soluble phosphorus (P) and make it available to plants. In this context, the aim of this study was to isolate and identify new strains of filamentous fungi from lichens and sediments from Antarctica with the ability to solubilize inorganic phosphate. Lichens and sediments from Antarctica were collected during the OPERANTAR XXXVI expedition, in the austral summer of 2017/18. The 6 lichens of different colors were collected in King George Island - Punta Ullmann and Punta Hanequin, and the 4 from sediments collected in Deception Island, Whalers Bay, due to the South Shetlands (Maritime Antarctica). A collection of 82 fungal strains were selected and evaluated for an inorganic phosphate solubilization activity. Make the total of 82 fungi tested, 3 lichen and 8 sediment grant, dissipation activity for phosphate solubilization in solid NBRIP culture medium containing tricalcium phosphate (TCP) at 15 °C. 6 isolates were selected during screening in solid medium with larger solubilization halos and tested in temperature ranges, varying between 15.0, 25.0 and 30.0 °C. In solid medium, the fungi were able to solubilize in the three temperature ranges, with a solubilization index (IS) ranging from 1.4 to 3.63 mm subjected to a temperature of 15 °C, from 1.3 to 3.57 mm to 25 °C and from 1.3 to 1.4 mm at 30 °C. One of the largest IS was found in isolates 1EM.P1 at 15 °C and 5Y.P4 at 25 °C with 3.63 and 3.57 mm, respectively. In a liquid medium, the 1EM.P1 isolate was the only one to present significant values for solubilization, with an average release of soluble P of 106.14 mg/L-1 and with a pH reduction from 7.0 to 4.38 to 15 °C, and at 25 °C with soluble P release of 80.89 mg/L-1 and pH reduction from 7.0 to 5.40. For tests under stress conditions, the 1EM.P1 isolate showed better solubilization when subjected to 0.5 M NaCl. Regarding the pH variation, it showed greater release of soluble P at pH 8.0. As for the carbohydrate source, it presented better solubilization in the presence of glucose. The 1EM.P1 isolate, when subjected to different sources of inorganic phosphate, showed better solubilization in the presence of TCP, with a maximum peak in 15 days of incubation. In general, the results showed that Antarctic fungi are able to solubilize phosphate in solid medium, as well as through the release of soluble P in the liquid medium, especially those isolated from sediment.

8
  • JARDIELSON DA SILVA SANTOS
  • EVALUATION OF THE PRODUCTIVITY OF IRRIGATED LETTUCE UNDER PROTECTED CULTIVATION IN RESPONSE TO THE WATER TREATMENT STATION SLUDGE APPLICATION

  • Advisor : WANDER GUSTAVO BOTERO
  • COMMITTEE MEMBERS :
  • ANDREA PIRES FERNANDES
  • MARCIO AURELIO LINS DOS SANTOS
  • WANDER GUSTAVO BOTERO
  • Data: Sep 10, 2021


  • Show Abstract
  • Water for human consumption must meet a potability standard in order to be used for drinking, food preparation and personal hygiene. For this, it is necessary that, regardless of its origin, the water is submitted to different physical and chemical treatments, or a combination of both. Water treatment consists basically in the removal of suspended and colloidal particles, organic matter, microorganisms, and other substances possibly harmful to human health. After the removal of particles a residue called sludge is generated, which is a mixture of sand and clay, rich in organic matter (OM), micro and macro nutrients. This waste has still been disposed in water courses without any previous treatment. For its reuse is of fundamental importance the study of the structural characteristics, as well as the evaluation of the OM content present in this waste. Thus, the objective of this study was to extract and characterize the OM of the sludge from the Water Treatment Plant in the city of Arapiraca, Alagoas, and then apply it to the lettuce crop in order to evaluate the influence of OM on plant growth and development. The samples were collected and submitted to drying and then disaggregated and sieved. The results obtained in this study indicate that this waste is rich in OM, indicating that this matrix has a potential as an agricultural additive. The E4/Eratio shows that the humic substances (HS) contain few aromatic structures and a lower degree of humification. The E2/Eratio showed a high value, indicating a decrease in the molecular size of the structures that compose the HS. From the E2/Eratio it can be inferred that in the studied SH, there is the presence of structures derived from lignin. The HS, contributed to the growth and development of the lettuce plants. For most of the variables analyzed, the HS showed a significant effect. The HS dose of 100 mg L-1 was the best response dose. A decrease in the effects of the analyzed variables was observed as the concentration of HS increased.

9
  • RAFAELA FERREIRA DA SILVA COSTA
  • SOLUBILIZATION OF INORGANIC PHOSPHATE BY BACTERIA ISOLATED FROM ANTARCTICA LICHEN

  • Advisor : ALYSSON WAGNER FERNANDES DUARTE
  • COMMITTEE MEMBERS :
  • ALYSSON WAGNER FERNANDES DUARTE
  • JOÃO GOMES DA COSTA
  • MICHEL RODRIGO ZAMBRANO PASSARINI
  • VALDEVAN ROSENDO DOS SANTOS
  • Data: Sep 10, 2021


  • Show Abstract
  • Lichens are pioneer organisms in extreme environments, grow in nutrient-limiting conditions and are recurrent in Antarctica. They represent a rich reservoir for the isolation of bacteria with biotechnological potential of agricultural interest, presenting characteristics that promote plant growth through inorganic phosphate solubilization mechanisms. In this context, the present study aimed to analyze the potential of bacteria isolated from 6 Antarctic lichens for solubilization of inorganic phosphate under the influence of different temperatures, pH variation, NaCl concentrations, carbohydrates and induction of osmotic potential. The collection took place from different lichens on King George Island, during the OPERANTAR XXXVI expedition (summer 2017/2018). Bacteria were tested for phosphate solubilization using solid NBRIP medium (qualitative) and in broth (quantitative). The positive bacteria for solubilization were tested for the ability to fix nitrogen. The quantification of soluble phosphorus in the supernatant of the isolates was estimated by the ammonium vanadate-molybdate method. Of 60 bacteria tested, 15 (25.0%) solubilized tricalcium phosphate at 15.0 ºC. At temperatures of 15.0 ºC (15= 100%) and 25.0 ºC (15= 100%) all strains solubilized phosphate in solid culture medium, whereas at 30.0 ºC 13 strains (13= 87. 5%) and at 35.0ºC there was no growth of any isolate. The enzyme index for phosphate solubilization was measured by the ratio of the diameter of the halo divided by the diameter of the colony. The highest phosphate solubilization indexes at 15.0 ºC were from 1.75 mm to 3.02; at 25.0 °C from 1.84 mm to 2.76 mm and at 30.0 °C from 1.78 mm to 2.70 mm. The best bacteria at the three temperatures were 1B.LQ4; 4BLQ4; 4BLQ4* and 2BLQ1*, together with 7BLQ2 (randomly chosen) were selected for phosphorus quantification in liquid medium at 25.0 ºC ± 2.0 ºC. Strains 1BLQ4 and 4BLQ4 had an average of 605.18 mg/L and reduced the pH to 4.07, while 2BLQ1* solubilized 505.56 mg/L and final pH of 4.29 and 4BLQ4* with 494.05 mg /L and pH 4.17. These four isolated showed no significant difference in soluble P. The lowest mean of soluble P was from strain 7BLQ2 with 365.78 mg/L and pH from 7.0 to 4.33. Of the 15 phosphate solubilizers, 80% (13) grew in selective medium for nitrogen fixatives and the strains that showed the greatest growth were 2BLQ1* and 3BLQ5. Bacteria 1BLQ4 and 2BLQ1* were selected for testing under stress conditions. Both bacteria solubilized phosphate at the concentration of 0.5 M Nacl with averages of 421.81 mg/L and 432.77 mg/L, respectively. At the different pH tested (7, 8 and 9), the two strains continued with high levels of soluble P. When subjected to tests with different carbohydrates (glucose, sucrose, lactose and starch), glucose was the most efficient for the solubilization mechanism of the two isolates. 2% glycerol was used; 5% and 10% to induce osmotic potential and both strains maintained high levels of soluble P in the 3 conditions tested. Assessing the maximum point of bacterial solubilization every 24 hours for 6 consecutive days, the best result for 1BLQ4 was with 96 h of incubation (606.45 mg/L) and for 2BLQ1 * it was with 120 h (565.41 mg/L) L). The results demonstrate that these strains are efficient in the solubilization mechanism of inorganic phosphate at different temperatures, alkaline conditions, osmotic effect and reasonable concentrations of salinity.

10
  • LARYSSA ROBERTA ALVES FARIAS
  • AGRONOMIC PERFORMANCE OF PEANUT CULTURE IRRIGATED WITH SALINE WATER IN A PROTECTED ENVIRONMENT

  • Advisor : MARCIO AURELIO LINS DOS SANTOS
  • COMMITTEE MEMBERS :
  • MARCIO AURELIO LINS DOS SANTOS
  • INAJÁ FRANCISCO DE SOUSA
  • DANIELLA PEREIRA DOS SANTOS
  • SAMUEL SILVA
  • Data: Sep 11, 2021


  • Show Abstract
  • The implementation of areas with irrigated peanuts in the region is still limited due to water availability and little knowledge of this technique for this culture in the region. The implementation of irrigation in the crop allows the cultivation of two or more peanut cycles during the year, thus increasing the income of small farmers who are the main producers. The objective of this research was to evaluate the agronomic performance of peanuts in a protected environment, under the effect of different water depths and different levels of water quality. The experiment was carried out in a greenhouse in the experimental area of the Arapiraca Campus at UFAL. The experimental design was randomized blocks (DBC), with 4 replications, in a 5x5 factorial scheme, with 25 treatments, totaling 100 plots. The treatments represented five irrigation depths L1=50; L2=75; L3=100; L4= 125 and L5= 150% of crop Evapotranspiration correlated with saline water levels represented by S1 = 0.15; S2 = 1.15; S3 = 2.15; S4 = 3.15 and S5 = 4.15 dS/m. The determination of ETc was obtained using SLIMCAP. The method of localized irrigation with a drip system with an average flow of 2.5 L/h per dripper was used. The variables were evaluated: plant height (AP), number of stalks per plant (NHP), number of pods per plant (NVP), average pod length (CMV), average pod diameter (DMV), number of grains per pod (NGV), pod fresh weight (PFV) and pod dry weight (PSV), chlorophyll index (SPAD). The collected data were subjected to statistical analysis and surface graph plots using the R i386 statistical software. The results showed that the variables plant height index (AP), number of pods per plant (NVP), average length of pods (CMV) and chlorophyll index (SPAD) respond to the interaction of irrigation depth and water salinity levels . The number of stalks per plant (NHP), mean diameter of pods (DMV), volume of pods per plant (VVP) and green weight of pods (PVG), dry weight of pods (PSG) respond to lamina and salinity separately. The number of grains per pod responds significantly only to water salinity. The maximum production points for most variables were obtained with irrigation depths below 100% of ETc and salinity close to 0.15 dS/m.

11
  • MARIA APARECIDA DOS SANTOS
  • ASSEMBLY, ANNOTATION OF PLASTIDIAL AND MITOCHONDRIAL GENOMES OF Stylosanthes seabrana and Stylosanthes guianensis

  • Advisor : ANDRE SECO MARQUES DA SILVA
  • COMMITTEE MEMBERS :
  • ANDRE SECO MARQUES DA SILVA
  • CICERO CARLOS DE SOUZA ALMEIDA
  • LUIS GUSTAVO RODRIGUES SOUZA
  • Data: Sep 16, 2021


  • Show Abstract
  • The genus Stylosanthes Sw. is highly diverse and polymorphic, many species are present in regions of low rainfall, grow in calcium-poor soils, are able to fix nitrogen in the soil, and are of great importance in the formation of pastures and also in the recovery of degraded areas. Despite its economic importance, the genus Stylosanthes still presents little genetic characterization and its taxonomy and systematic complexity makes the delimitation and identification of species difficult. Organellar genomes, such as those of chloroplast and mitochondria, can be very useful in resolving phylogenetic relationships in groups with complex systematics. Due to their uniparental, mainly maternal inheritance in angiosperms. The objective of this work was to obtain and analyze comparatively the complete Plastome and Mitogenome of two species of the genus Stylosanthes: S. seabrana, S. guianensis in order to increase the knowledge about the evolutionary relationships and genetic improvement of the genus. The genomes of each species were assembled using NOVOPlasty v. 3.8.3 software, using as reference the plastidial and mitochondrial genome of Stylosanthes hamata. After assembly, with the help of Geneious v. 9.1.8 software, the genomes were analyzed and compared. The circular plastidial genome for Stylosanthes seabrana showed a total size of 156,499 bp, and a total of 125 genes were identified. In turn, the plastidial genome of Stylosanthes guianensis showed a total size of 156,785 bp, being identified a total of 127 genes. The mitochondrial circular genomes obtained for the two species presented a length of 468,896 bp in S. guianensis, being identified 55 genes and in S.seabrana of 523,870 bp, being identified 57 genes. The plastid and mitochondrial genomes of the species of the genus Stylosanthes analyzed in this study proved to be highly conserved and can be used for further phylogenetic studies and contribute to the genetic improvement programs of these species.

12
  • FERNANDA DANIELLE DE SOUSA

  • The complete mitochondrial genome of the Hancornia speciosa Gomes and evolutive analysis in Apocynaceae

  • Advisor : CICERO CARLOS DE SOUZA ALMEIDA
  • COMMITTEE MEMBERS :
  • CICERO CARLOS DE SOUZA ALMEIDA
  • ANDRE SECO MARQUES DA SILVA
  • LUIS GUSTAVO RODRIGUES SOUZA
  • Data: Sep 17, 2021


  • Show Abstract
  • Hancornia is a monotypic genus that belongs to the Apocynaceae family, its only species is an H. speciosa Gomes, also known as mangaba. It is a fruit tree, native to Brazil, appreciated for its sweet and slightly acidic flavor that makes it quite characteristic. In addition to its ecological and industrial importance, it is a source of income for agro-extractivists. The mitochondrial genome of plants has important evolutionary characteristics, such as the large size of intergenic spacers and a large number of rearrangements, and has been studied in many groups of plants. In order to understand the phylogenetic relationships, evolutionary aspects and contribute to research on plant breeding, the mitochondrial genome of Hancornia speciosa was sequenced by Next Generation Sequencing (NGS). And through the de novo assembly and union of contigs, the complete mitochondrial genome was obtained with a size of 741,811 bp in length and 63 annotated genes, becoming the longest reported in Apocynaceae. In summary, we demonstrate: (1) the first mitochondrial genome of Hancornia speciosa; (2) The incorporation of chloroplast DNA into the mitochondrial genome in Hancornia speciosa is smaller than Rhazya stricta, Hoya lithophytica and Asclepias syriaca and its expansion may be associated with recombination events and increase in the intergenic region; (3) The synteny between H. speciosa and Rhazya stricta, which belong to the Rauvolfioideae subfamily, and between Hoya lithophytica and Asclepias syriaca, which belong to the Asclepiadoideae subfamily, show remarkable rearrangements; (4) The association of data obtained between mitochondrial rearrangements and plastid nucleotide sequences reveals that the closer genomes share more regions in common. Comparative analyzes show the remarkable diversity of the mitochondrial genome already reported in angiosperms, which shows high conservation of genes in their functioning and high structural plasticity.

13
  • NATANIELLE DE OLIVEIRA GOMES LEITE
  • BIOSPECKLE TECHNIQUE APPLICATION IN THE MORPHO-PHYSIOLOGICAL CHARACTERIZATION OF AUREA MART Tabebuia leaves. IN TWO LIGHT AND WATER CONDITIONS

  • Advisor : JOSE VIEIRA SILVA
  • COMMITTEE MEMBERS :
  • EMERSON DE LIMA
  • JAKSON LEITE
  • JOSE VIEIRA SILVA
  • RUBENS PESSOA DE BARROS
  • Data: Sep 20, 2021


  • Show Abstract
  • The biospeckle technique has been used as a tool for analyzing the biological materials activity, monitoring moisture, activity level and presence of parasites, through of the material illumination by a low power laser source. As it is a non-destructive technique, the biospeckle is an advantageous tool in fast procedures and with great precision, especially in the biological systems analysis. Thus, the objective of this research was to identify and characterize the leaf morphophysiology of Tabebuia aurea (Mart.) species submitted to water restrictions and the application of a particle film using biospeckle technique. The experiment was conducted at the Plant Physiology (LABFIVE) and Biospeckle (LEB) Laboratories and in a greenhouse at the Federal University of Alagoas, Campus Arapiraca. The experimental design was in randomized blocks (DBC) with 4 treatments [irrigated + presence of film; irrigated + absence of film; non-irrigated + film presence; non-irrigated + no film] with 4 repetitions for each treatment. Tabebuia aurea (Mart.) seeds collected from matrix trees located on the Campus Arapiraca of the Federal University of Alagoas - UFAL, were germinated in polyethylene pots with a capacity of 3L in a greenhouse (with shading of ~ 50%) until establishment of the seedlings. After the growth and seedlings establishment, the plants were transferred in the early morning hours (between 5:30 and 6:00 a.m.) to the environment outside the greenhouse under full sun, and subjected to treatments. After the treatments imposition, the evaluations were carried out for a period of 30 days. In which, the morphological and biological activity analyzes in the leaves were started at time 0, 24 hours (one day), 48 hours (two days), 72 hours (three days), seven days, 15 days and 30 days. The collected data were submitted to statistical analysis and graph plotting using the R i386 statistical software. The variables plant height index (AP), stem diameter (mm), and number of leaves in Tabebuia aurea (Mart.) plants, as well as biological activity (MDI) and homogeneity responded significantly between treatments irrigation (with and without) in the presence or not of the particulate film.

14
  • Fabiana Fontes Silva Macedo de Carvalho
  • NUTRITIONAL CHARACTERISTICS AND FORAGE PALM BIOMASS ACCUMULATION (Nopalea cochenilifera (L.) Salm-Dyck) INFLUENCED BY STYLOSANTHES AND ORGANIC FERTILIZATION.

  • Advisor : CICERO GOMES DOS SANTOS
  • COMMITTEE MEMBERS :
  • CICERO GOMES DOS SANTOS
  • MARCIO AURELIO LINS DOS SANTOS
  • KEDES PAULO PEREIRA
  • CAROLINA CORRÊA DE FIGUEIREDO MONTEIRO
  • Data: Sep 24, 2021


  • Show Abstract
  • The forage cactus (Nopalea cochenillifera (L.) Salm – Dick) has morphophysiological characteristics that allow its development in conditions of low water supply, and has played an important role in livestock in the Semi-arid region, forming the basis of ruminant feeding. The production of forage cactus in the Northeast of Brazil presents low productivity mainly due to inadequate management, lack of correction of nutritional levels and low levels of organic matter in the soil. The use of Stylosanthes species as green manure can favor the production system of forage cactus, working as an alternative source of nutrients, especially nitrogen, to inorganic fertilizers. The contribution of plant residues to the soil favors the increase in organic matter content, nutrient cycling, microorganism activity, structuring, infiltration and storage of water in the soil, improving the quality and resilience of agroecosystems. This work aimed to evaluate the nutritional composition and biomass accumulation of forage cactus cultivated in the residue of stylosanthes cv. Campo Grande as green manure and organic manure using cattle manure. The experiment was carried out in the experimental area of the Federal University of Alagoas - UFAL, Campus Arapiraca, in randomized blocks, with four replications, in a 3x2 factorial scheme. The first factor studied corresponded to fertilization: a) green fertilization with stylosanthes cv. Campo Grande associated with chemical phosphate fertilization, b) organic fertilization with cattle manure associated with chemical phosphate fertilization, and c) control with chemical phosphate fertilization without green and organic fertilization. The second factor studied was spacing: 1.20 x 0.20 and 1.20 x 0.25, with plant density per area of 41,000 and 33,000 plants per hectare, respectively. Biometric variables were evaluated: plant height (AP), plant width (LP) and number of cladodes emitted per plant (NC), starting at 180 days after planting (DAP), with an interval of 60 days between measurements; chemical-bromatological and mineral composition; chlorophyll and carotenoid contents in two distinct periods; and production of green and dry biomass in 12 months. To perform the statistical analysis, the SISVAR software, version 5.6 was used. The variables studied show significant differences in relation to treatments with green and organic fertilizers, and significant biomass production with organic fertilizer and 1.20 x 0.20 spacing in relation to the other treatments

15
  • JÂNIO PEDRO DA SILVA VIEIRA
  • BIOMASS, DECOMPOSITION AND NUTRIENT RELEASE OF SHOOT COVER CROPS IN THE AGRESTE REGION, ALAGOAS, BRAZIL

  • Advisor : VALDEVAN ROSENDO DOS SANTOS
  • COMMITTEE MEMBERS :
  • VALDEVAN ROSENDO DOS SANTOS
  • GILSON MOURA FILHO
  • RUBENS PESSOA DE BARROS
  • Data: Sep 27, 2021


  • Show Abstract
  • Green manure consists in the cultivation of different plant species in the same area, in succession or simultaneously, with the main purpose of improving soil fertility. Thus, it is preferred that those that produce a greater volume of dry matter, are less subject to pests and diseases and that have relatively uniform and easy to sow seeds. The Fabaceae family plants are the most used in green manure, due to their high potential to carry out the fixation of atmospheric nitrogen through symbiosis with bacteria present in nodules located in plant roots, and their growing capacity to store biomass and nutrients, characterizing itself as one of the most suitable methods for use in agriculture. The aim of the present study was to evaluate biomass production, decomposition rates and nutrient release dynamics from the aboveground part of cover crops in the Agreste region, Alagoas, Brazil. The experiment was carried out under field conditions, in the experimental area of the Federal University of Alagoas - Campus Arapiraca, in randomized blocks, with four replications. The treatments consisted of cover crops: sunn hemp (Crotalaria juncea), Spectabilis (Crotalaria spectabilis), dwarf pigeon pea (Cajanus cajan), lablab (Dolichos lablab), jack bean (Canavalia ensiformis), millet (Pennisetum glaucum) and black velvet bean (Mucuna aterrima). The decomposition and release of nutrients from the residues was monitored through periodic collections at 30, 60, 90, 120 and 150 days after the incorporation of litter bags in the soil containing 20 grams of dry mass. Biomass production, extraction and nutrient use efficiency, and the decomposition and release of nutrients from waste were evaluated. The studied variables show significant differences in relation to nutrient concentrations, C/N and C/P ratio in dry mass, yield, extraction and nutrient use efficiency by the aerial part of cover crops in full bloom. Decomposition rates and half-life times varied with the cover crop species, with jack bean being the species with the highest rates and half-life times.

16
  • ALEYRES BISPO CHAGAS
  • Study of biological activity in Enterolobium Contortisiliquum (Vell.) Morong seeds using the biospeckle technique

  • Advisor : JOSE VIEIRA SILVA
  • COMMITTEE MEMBERS :
  • EMERSON DE LIMA
  • FLAVIA DE BARROS PRADO MOURA
  • JOSE VIEIRA SILVA
  • RUBENS PESSOA DE BARROS
  • Data: Sep 28, 2021


  • Show Abstract
  • The biospeckle laser technique is based on an optical interference phenomenon that happens when a coherent light beam falls on the material surface, where there is activity of biological origin. Thus, the objective of this work was to characterize the metabolic activity through the biospeckle technique, correlating it with the seed imbibition curve after chemical and mechanical scarification and at different times. For this, newly collected E. contortisiliquum seeds were subjected to five different treatments, namely Control, H2SO4(PA) at 5 minutes, H2SO4(PA) at 10 minutes, H2SO4(PA) at 15 minutes and Mechanical Scarification, and exposed at soak times of 0, 8, and 72 hours. The experiment was carried out in a completely randomized design and all treatments consisted of five replicates consisting of 100 seeds each. The seeds were weighed on an analytical balance with a precision of 0.001 g before and after imbibition and an imbibition curve was established. For the analysis of the metabolic activity of seeds by the biospeckle technique, newly collected E. contortisiliquum seeds were used and submitted to the same five treatments described above, and soaking time of 0, 4 and 72 hours, with five replicates of 57 seeds. The TCAPTURE, COMMAND PROMPT, STDM programs were used, and among the lines that form the Y axis of the matrix, the line 100 was established as the standard line for the study of changes occurring in a region of the sample surface. The analysis of the speckle patterns temporal evolution was performed using a THSP image, followed by the calculation of the MCO. Chemical scarification treatments with concentrated sulfuric acid proved to be efficient in overcoming seed tegumentary dormancy, since increasing imbibition occurred with times of 0, 8 and 72 hours. The seeds imbibition resulted in the primary root emission and integument disruption at the base of the seed, close to the hilum; the primary root showed a pivoting shape, light yellow in color and with few simple hairs. Soon after, from 48 to 72 hours, the tegument detachment occurred, leaving the fleshy cotyledons free, characterizing a phanerocotyledonary epigeal germination, with the control treatment exception. The hypocotyl is elongated and straight, with a light grayish-green coloration. The epicotyl is grayish-green yellow in color. The eophylls are composed, opposite, bipinnate, and have a dark yellow grayish-green color. The development of uniform seedlings, with the control treatment exception, which had little water absorption by the seeds. They are also evaluated using the biospeckle technique where the calculated descriptors are defined and mathematically formalized, by measuring the homogeneity or uniformity of the texture. This results in higher values when the intensities are uniform and the Inertia (MI) was more efficient in the analysis of high frequencies, which is due to its actuation mechanism, which is a second order moment, which favors variations in highest activity.

17
  • JANYNNE JOYCE DE LIMA ROCHA
  • Changes in physical and chemical soil attributes as a function of land use in the municipality of Limoeiro de Anadia - AL

  • Advisor : CARLOS TADEU DOS SANTOS DIAS
  • COMMITTEE MEMBERS :
  • CARLOS TADEU DOS SANTOS DIAS
  • VALDEVAN ROSENDO DOS SANTOS
  • GILSON MOURA FILHO
  • RUBENS PESSOA DE BARROS
  • Data: Oct 29, 2021


  • Show Abstract
  • The study of changes in the physical and chemical attributes of the soil allows establishing parameters capable of assessing soil quality. The objective of this work was to test the hypothesis that the intensification of agriculture through the cultivation of sugarcane, corn and pasture alters physical and chemical properties of the soil, when compared to land use with native vegetation. Soil samples were collected in four areas (1 hectare each, with 12 sampling points) in three layers (0.0 – 0.10; 0.10 – 0.20; 0.20 – 0.30 m ) in the municipality of Anadia Lemon Tree (Alagoas State) and analyzed for acidity and CTC properties (pH, CTCpH7, base saturation, aluminum saturation, Al+H and Na), macronutrients (P, K, Ca and Mg) and attributes physical (soil texture, soil density, particle density, total porosity, soil mechanical resistance to penetration and aggregate stability). Data were analyzed using statistical programs.

2020
Dissertations
1
  • ALAIDE MARIA SILVA SANTOS
  • Beneficial effects of endophytic bacteria isolated from caatinga leguminous nodules on maize growth

  • Advisor : FLAVIA DE BARROS PRADO MOURA
  • COMMITTEE MEMBERS :
  • FLAVIA DE BARROS PRADO MOURA
  • ALYSSON WAGNER FERNANDES DUARTE
  • ESMERALDA APARECIDA PORTO LOPES
  • JAKSON LEITE
  • Data: Feb 18, 2020


  • Show Abstract
  • Endophytic bacteria from legume nodules have been widely isolated in several studies. However, the potential of these bacteria to promote plant growth is still little explored. This paper aimed to identify the potential of promoting plant growth in bacterial isolates from nodules of legumes from the caatinga. For this, three experiments were assembled out with different levels of complexity. The three experiments performed the same processes of growing the inoculum at a concentration of 5.2 x 107 UFC mL-1 and disinfesting the maize seeds. A total of 14 bacteria isolated from legume nodules were identified by sequencing the 16S RNA gene and tested. In the first experiment, the seeds remained 1 hour in immersion of the bacterial growth (5.2 x 107 CFU mL-1 ) corresponding to each treatment and the control without inoculum immersed in culture middle, immediately after they were placed on sheets of germitest paper and inserted. The variables analyzed were root length and number of lateral roots at four and eight days. The second experiment was carried out in a greenhouse in DBC, the treatments corresponded to 14 bacteria and two controls, one without inoculation and one with nitrogen fertilization (15 mg of N), the substrate used was vermiculite and the seeds were inoculated with 1 mL of bacterial growth. The variables analyzed were green intensity, root dry matter, aerial part dry matter and total dry matter. The third experiment had 12 treatments, 9 bacteria plus the addition of 35 kg ha-1 of N, three controls (without inoculum, one with 35 kg ha-1 of N and one with 70 kg ha-1 of N), the seeds were inoculated with 1.2 mL of bacterial growth. The variables analyzed were green intensity, height, dry shoot weight and nutrient accumulation. Regarding the first experiment, 13 bacterial strains promoted (p <0.005) greater root growth when compared to the control, in relation to the number of lateral roots, the bacteria seemed to inhibit the increase. The strains represented the genera Bacillus (4), Brevundimonas (1), Enterobacter (1), Leifsonia (2), Pseudomonas (1), Paenibacillus (2) and Roseomonas (2). In the second experiment, the green intensity (SPAD index), dry root mass (MSR), dry shoot weight (MSPA) and total dry mass (MST) were determined at 30 days after emergence. The strain Bacillus ShS9 promoted an increase (p <0.005) in the SPAD and MSR index. Bacillus ShS3 promoted (p <0.005) MSR and MPAS. Roseomonas sp. SsP7 induced an increase in (p <0.005) MSR and the strain Bacillus AD47 overcame control and promoted MSPA. All strains promoted MST and root length. In the third experiment, the strain Paenibacillus SsS3 promoted an increase (p <0.005) of plant height and MPAS, surpassing the control with 70 kg ha-1 of N. The isolates Roseomonas SsP7, Pseudomonas ShP11, Brevundiomonas ShS2 and Bacillus AD47 promoted an increase (p <0.005) in MPAS, showing performance similar to the treatment with 70 kg ha-1 of N. These same strains promoted the accumulation of mineral nutrients in maize plants. The better performance of isolates of the genus Bacillus confirms what is found in many studies, that this genus is directly related to the promotion of plant growth. Endophytic bacteria from nodules of tree legumes that are not rhizobia can promote the growth of maize plants. Additional studies need to be conducted to elucidate the mechanisms that these bacterial isolates use to promote the growth of corn plants.

2
  • JOSELIANE FERNANDES MIGUEL DOS SANTOS
  • COWPEA BEAN RESPONSE TO THE ACTION OF NITROGEN FIXING BACTERIA IN DIFFERENT HIDRIC SCENARIOS

  • Advisor : FLAVIA DE BARROS PRADO MOURA
  • COMMITTEE MEMBERS :
  • FLAVIA DE BARROS PRADO MOURA
  • VALDEVAN ROSENDO DOS SANTOS
  • ADAILSON PEREIRA DE SOUZA
  • JAKSON LEITE
  • Data: Feb 18, 2020


  • Show Abstract
  • Cowpea (Vigna unguiculata (L.) Walp.) can be grown under different edaphoclimatic conditions. This is due to its excellent ability to adapt to tropical conditions. It is considered one of the main subsistence crops for the Brazilian Northeast, with a high protein and energy content. As it is a culture with high socioeconomic value, it plays an important role in the capitalization of small farmers in this region. Cowpea is a legume with a great capacity to acquire nitrogen via biological nitrogen fixation (FBN) when in symbiosis with specific and effective bacteria. This process allows to increase the production of this legume and reduces the costs with nitrogen fertilizer. The objective of this work was to evaluate the response of cowpea grown with different sources of nitrogen and water availability. The experiment was conducted under greenhouse conditions in a tow-way factorial design with and without water stress and five N sources with each treatment repeated six time in random blocks. The nitrogen sources corresponded to the inoculation with three strains of Bradyrhizobium sp. (AD4, AD8 and AP17), mineral fertilizer (30 kg ha-1) and control (without inoculation and fertilization). Each isolate corresponded to a treatment and 1 mL of bacterial growth (10-9) was inoculated on the seed at the time of sowing. At 20 days after emergence (DAE) half of the experiment was subjected to water stress with total cut of irrigation for 10 days. After this period, the plants were irrigated for 5 days, and plants were collected at 35 DAE. The effects of the treatments were evaluated based on the variable nodules number (NN, nº plant-1), nodule dry weight (MSN), shoot dry weight (MSPA, g plant-1), leaf green intensity ( SPAD index), shoot N, P e K content. After ten days of water suspension, leaf gas exchange was evaluated by stomatal conductance (gs), liquid photosynthesis rate (A), transpiration (E), internal carbon concentration (Ci) and, even on this same date, chlorophyll a fluorescence was performed by the Fv / Fm ratio. There was no significant effect for NN and MNS. AD4 and AP17 sources promoted higher MSPA. The quantum efficiency of photosystem II (Fv / Fm) was not affected during the water restriction period. There was a reduction in gs stomatal conductance, liquid photosynthesis (A) and transpiration (E). There was no significant effect for P. AD4 provided greater accumulation of N and K in the scenario without water restriction.

3
  • MARIA ALICE SILVA OLIVEIRA
  • GENOMIC CHARACTERIZATION OF REPETITIVE SEQUENCES IN DIPLOID AND POLYPLOID SPECIES OF THE GENUS Stylosanthes Sw.

  • Advisor : ANDRE SECO MARQUES DA SILVA
  • COMMITTEE MEMBERS :
  • ANDRE SECO MARQUES DA SILVA
  • CICERO CARLOS DE SOUZA ALMEIDA
  • JAKSON LEITE
  • Data: Feb 20, 2020


  • Show Abstract
  • The nuclear genome corresponds to the largest fraction of DNA in eukaryotes, and is inserted as repetitive DNA sequences, which can correspond to up to 90% of this genome. As repetitive sequences are great allies in evolutionary studies, they help to understand the organization and the evolutionary behavior of eukaryotic genomes. The advancement of next generation sequencing facilitates the use of a large number of sequences and more affordable prices, enabling the discovery of repetitive sequences. Among the methodologies that emerged with this advent is the RepeatExplorer program, a tool with high potential in the discovery of these sequences. The genus Stylosanthes Sw. is part of the Leguminosae family and despite having high economic and environmental value it still has a low genetic characterization, due to the occurrence of hybrid species and high systematic complexity. It is a diverse genre with wide distribution, with Brazil as its main center of origin and diversity. The study sought to understand the organization of the nuclear genome of nine species of the genus Stylosanthes Sw., including two allopolyploid complexes of economic interest (Complex S. scabra and Complex S. capitata), through the genomic analysis of repetitive sequences, seeking to characterize the abundance of these sequences and understand the phylogenomic relationships between diploids and allopolyploids. The genomic reads of the species S. hamata, S. viscosa and S. scabra were obtained from the study by Marques et al. (2018), available at NCBI, and for the other species sequencing was done by GenOne Solutions in Biotechnology, Rio de Janeiro, Brazil. The reads of the nine species  were inserted into the Geneious software, where they were paired, converted into a fasta format and selected. The obtained sequences were concatenated and inserted in the RepeatExplorer program, being characterized individually and comparatively, through the RepeatExplorer2 version and the TAREAN tool. A phylogenetic analysis was also carried out using  the satellite of each species, using the AAF program. The RepeatExplorer analyzes revealed that the nine studied Stylosanthes species have more than 50% of their genome formed by repetitive sequences, with the class of transposable elements of the LTR order being the most abundant. The abundance of satellite DNA varied between species, and the grouping of reads in reading charts revealed good conservation of the sequences, representing low diversification in the evolutionary process. Phylogenetic analysis made it possible to assemble a tree with kinship relationships between the enlightened species and supporting previous studies. Unlike what the literature shows us, our study revealed a close relationship between the species S. hamata and S. seabrana, which may be synonymous in the process of speciation. The differences found between the results and what appears in the literature reinforces the need for more in-depth studies that review the taxonomy and phylogenetics of the group, especially in the molecularscope.

4
  • MARIA GILBERLÂNDIA FERREIRA FERRO
  • AGROINDUSTRIAL PERFORMANCE OF SWEET CORN UNDER FERTILIZER
     LEVELS OF SLOW RELEASE IN ASSOCIATION WITH Azospirillum brasilense
  • Advisor : ADEMARIA APARECIDA DE SOUZA
  • COMMITTEE MEMBERS :
  • ADEMARIA APARECIDA DE SOUZA
  • VALDEVAN ROSENDO DOS SANTOS
  • ÁLVARO CARLOS GONÇALVES NETO
  • Data: May 25, 2020


  • Show Abstract
  • The culture of sweet corn has high production potential and socioeconomic importance, since its grains have excellent sensory characteristics for industrial processing and even for consumption in natura. Despite the importance, technical information is still scarce, therefore, research aimed at a rational productive system, mainly regarding the use of slow release fertilizers , associated with plant growth promoting bacteria, can result in significant gains. In this sense, the present study aimed to evaluate the influence of slow-release fertilizer, in association with Azospirillum brasilense bacteria on the agro-industrial characteristics of sweet corn culture. The experiment was conducted in the experimental area of Indústria Hada/Asa Branca, in the municipality of Arapiraca - AL, from June to September 2018. The experimental design used was in randomized blocks in a factorial scheme (5x2) + 2 additional, represented by five doses of slow-release fertilizer applied to the foundation, two conditions of inoculation with the bacterium (presence and absence), and two additional treatments (conventional fertilization in the presence and absence of inoculation with the bacterium), with four replications, totaling 48 plots. The agro-industrial assessments were: luminosity index, number of tillers, stem diameter, height of the cob insertion, plant height, weight of the cob stuffed before cooking, weight of the with straw cob before cooking and winthout straw, length of the cob winth straw and number of rows of cob, number of rows of on the cob, grain number per row, number of grain per cob, grain depth and grain width, grain weight, pH, ° Brix, Proctivity of with cobs straw and without straw per hectare; and grain productivity per hectare. The data obtained were submitted to analysis of variance of the F test (p <0.05) and the significant effects studied by means of regression analysis (fertilizer doses) and by the Tukey test (bacterial inoculation), at 5% probability, with the aid of the Agroestat statistical program. The inoculation of bacteria does not interfere with the yield of sweet corn grains. The dose of 160 kg ha-1 slow-release fertilizer provides greater productivity of ears and grains of the sweet corn culture

5
  • LAYLTON DE ALBUQUERQUE SANTOS
  • Palma forrageira sobre a influência de diferentes lâminas de irrigação e níveis de salinidade

  • Advisor : MARCIO AURELIO LINS DOS SANTOS
  • COMMITTEE MEMBERS :
  • ABELARDO ANTÔNIO ASSUNÇÃO MONTENEGRO
  • GILSON MOURA FILHO
  • MARCIO AURELIO LINS DOS SANTOS
  • VALDEVAN ROSENDO DOS SANTOS
  • Data: Jun 15, 2020


  • Show Abstract
  • Objetivou-se avaliar o desenvolvimento da palma forrageira sobre a influência de diferentes lâminas de irrigação e níveis de condutividade elétrica na água. O experimento foi realizado em ambiente protegido, utilizando a cultivar Miúda, entre os meses de abril e novembro de 2019, na Universidade Federal de Alagoas, Campus Arapiraca. O delineamento utilizado foi blocos casualizados, com quatro repetições e dois fatores. No fator salinidade, as condutividades elétricas da água nos tratamentos eram de 0,19 (controle); 1,69; 3,25; 4,69 e 6,11 dS m-1 e no fator irrigação, as lâminas de água correspondiam a 25; 50; 75; 100 (controle) e 125% da evapotranspiração da cultura (ETc). Foram analisadas, pelo teste F, a massa fresca da planta e a interação entre suas ordens, variáveis biométricas dos cladódios (massa fresca, altura, largura, espessura e quantidade de água) e a quantificação de nutrientes (acúmulo pela planta e concentração no tecido de nitrogênio, fósforo, potássio, sódio e cloro). Verificou-se que os fatores não foram significativos (p > 0,05%) para altura e largura do cladódio e para a absorção e concentração de sódio. As variáveis significativas foram submetidas à regressão e o modelo matemático que melhor as representou foi escolhido. Houve interação entre os fatores para a massa fresca da planta (MFP). Os níveis salinos foram significativos, resultando em espessura e quantidade de água menores no cladódio, diminuição no acúmulo e concentração de nitrogênio, fósforo, incremento no acúmulo de potássio e elevação na concentração de potássio e cloro, com redução em elevadas condutividades elétricas. As lâminas de irrigação promoveram o aumento do número de cladódios e no acúmulo de nitrogênio e fósforo. A palma forrageira cv. Miúda foi sensível a salinidade da água de irrigação.

6
  • RONY DE HOLANDA COSTA
  • Agronomic Performance of Peppers Pulse Irrigated

  • Advisor : MARCIO AURELIO LINS DOS SANTOS
  • COMMITTEE MEMBERS :
  • MARCIO AURELIO LINS DOS SANTOS
  • CICERO GOMES DOS SANTOS
  • CÉLIA SILVA DOS SANTOS
  • GERÔNIMO FERREIRA DA SILVA
  • Data: Jul 6, 2020


  • Show Abstract
  • The  culture of  sweet  pepper  (Capsicum  annum L).  It  is  one  of  the  main  vegetables grown in Brazil, due to its economic return and for being appreciated in gastronomy, it has been gaining prominence in the northeast region of the country, being a great option for income in  commercial and family production.  Drip  irrigation is responsible for  the best  adaptation  of  the  crop,  since  the  water  resource  is  increasingly  scarce, leading  to  increased  production  and  efficiency  in  irrigation  management.  The objective was to evaluate the irrigation yield by pulsed drip in  peppers grown in  the field. The experiment was carried out in two seasons, a crop in the water period from May to July and another crop in the dry season from August to October 2019, in the experimental area of Grupo Irriga at the Arapiraca Campus of the Federal University of  Alagoas.  The  experimental  design  used  in  randomized  blocks  (DBC),  with  7 replications  and  5  plots  containing  7  plants  each.  The  treatments  consisted  of  5 irrigation pulses with an application interval of 60 minutes between each pulse: Pulse 1 (continuous pulse), Pulse 2, fractionation of the irrigation blade in two applications; Pulse 3, in three applications; Pulse 4, in four applications and Pulse 5, in five daily applications.  The  variables  analyzed  were  biometric: fruit  diameter  (FD),  fruit height (FH),  fruit  circumference  (FC),  fruit  volume  (FV),  fruit  wall  thickness  (FWT),  and productive  variables:  number  of  fruits  per  plant  (NFP),  fresh  fruit  weight  (FFW),  dry fruit  weight  (DFW),  water  content  in  the  fruit  (WCF),  pepper  productivity  (PP)  and water  use  efficiency  (WUE).  The  data  obtained  were  subjected  to  analysis  of variance  and  a  regression  was  generated  using  the  statistical  software  R®.  The analysis  of  the  biometric  and  productive  variables  of  the  pepper  showed  a  higher yield  during  the  rainy  season,  showing  the  importance  of  irrigation  during  rainy seasons.  The  irrigation  pulses  had  the  greatest  variations  in  the  dry  season,  with greater  magnitudes  in  the  2nd  harvest.  In  view  of  the  principal  component  analysis (PCA) applied to the two evaluation periods, it is recommended to split the irrigation only  for  the  dry  season.  During  the  rainy  season,  the  best  performance  of  the variables  when  analyzing  the  main  component  (PCA1,  PCA2  and  PCA3)  was obtained with the continuous pulse  (P1) and, in  general, with P5 during the drought period. The hierarchical cluster analysis showed that the variable fruit volume, in the two  harvests  and  in  the  two  growing  seasons,  stood  out  from  the  other  variables analyzed.

7
  • AVERLANE VIEIRA DA SILVA
  • Phosphate solubilization and activity of enzymes of agricultural interest by microorganisms from
    Antarctic lichens

  • Advisor : ALYSSON WAGNER FERNANDES DUARTE
  • COMMITTEE MEMBERS :
  • ALYSSON WAGNER FERNANDES DUARTE
  • VALDEVAN ROSENDO DOS SANTOS
  • MICHEL RODRIGO ZAMBRANO PASSARINI
  • Data: Jul 8, 2020


  • Show Abstract
  • The Antarctic continent is characterized by restrictive environmental conditions of climate, habitats and biogeography, presenting the coldest and driest climate known on Earth. In this regard, it is suspected that bacteria and yeasts adapted to such conditions, play key roles in increasing the availability of soil phosphorus for plants, standing out for its ability to solubilize sources of insoluble inorganic phosphates, as well as with the potential for producing active enzymes at mild temperatures. In this context, the objective of this study was to evaluate the potential of microorganisms isolated from Antarctic lichens for phosphate solubilization and prospecting for enzymes (celulase, amilase e pectinase). Lichen samples were collected on the islands of the South Shetland archipelago during the Brazilian Expedition to the Antarctic Continent, OPERANTAR XXIV (summer 2015/2016). A collection of 271 isolates previously recovered from 30 lichen samples from the Antarctic environment of the genera Usnea, Xanthoria, Sphaerophorus, Mastodia, Caloplaca, Umbilicaria, Lecania, Rhizocarpon and Cladonia was evaluated for inorganic phosphate solubilization. Of the total of 124 bacteria and 147 yeasts tested, 66 (53%) and 43 (29%), respectively, showed activity in solid NBRIP culture medium containing Ca3(PO4)2 at 15.0 °C. Most of the positive bacteria were of the genera Pseudomonas, Caballeronia, Chryseobacterium, Nakamurella and Rahnella, while the yeasts belonging to the genera Vishniacozyma, Mrakia, Cystobasidium and Holtermanniella. 12 bacterial isolates were selected from assays of phosphate solubilizing activity at different temperatures at 20.0 25.0 and 30.0 °C in solid medium, and it was observed that the bacteria Pseudomonas sp. 11.LB15 and 1.LB34 (not identified - N.I.D) grew and solubilized phosphate, under the same conditions, from 12 tested yeasts, two Vishniacozyma victoriae 2.L15 and A.L6 (N.I.D), also solubilized the inorganic phosphate. In liquid medium, bacterial isolates 11.LB15 and 1.LB34 at 25.0 °C, reached a maximum solubilization of 511.2 and 532.07 mg/L, at 122 and 101 hours, respectively. At 30.0 °C, these isolates solubilized 639.43 and 518.95 mg/L in 105 and 147 hours for 11.LB15 and 1.LB34, respectively. The yeast V. victoriae 2.L15 stood out at this stage, with a solubilization of 619.92 mg/L at 30.0 °C. Solubilization of insoluble phosphate was associated with a decrease in the pH of the culture medium. For the enzymatic test, three enzymes of agricultural interest were selected and the potential of the isolates in the hydrolysis of the enzymes was evaluated. Of 12 bacterial isolates tested, 8 showed enzymatic activity for pectinase, with halos ranging from 14.0 and 13.5 mm, for Pseudomonas sp. 1.LB10 and 11.LB15, respectively, followed by an amylase-producing isolate, 2.LB10 (N.I.D), which presented a 27.33 mm halo. Of the 12 yeasts tested, 5 showed activity for pectinase, with the largest halo for Vishniacozyma sp. 12.L3 (11.67 mm), and 5 isolates showed cellulase activity, with a greater halo for V. victoriae 2.L15 (27.33 mm). In general, the results showed that such microorganisms from Antarctica were able to solubilize the phosphate in a solid medium, as well as through the release of soluble phosphate in the liquid medium and produce enzymes of biotechnological and agricultural interest.

8
  • ITALA CARLA DE AMORIM NETO
  • Influence of calcium and magnesium enriched humin and water depths in two types of soil cultivated with lettuce

  • Advisor : WANDER GUSTAVO BOTERO
  • COMMITTEE MEMBERS :
  • WANDER GUSTAVO BOTERO
  • CICERO GOMES DOS SANTOS
  • MARCIO AURELIO LINS DOS SANTOS
  • ANDREA PIRES FERNANDES
  • Data: Jul 17, 2020


  • Show Abstract
  • Humin, a residue from the extraction of organic matter from soils with a strong base, has been shown  to  be  interesting  and  promising  as  an  adsorbent  of  environmental  interest,  due  to  its high  surface  area  and  porosity,  in  addition  to  the  diversity  of  functional  groups.  In  this context, the objective of this work was to develop a material from the humin fraction enriched with essential nutrients to plants (Ca2+ and Mg2+), and to evaluate its application in agricultural sectors  (lettuce  culture)  in  two  different  types  of  soil.  The  material  was  produced  from  the extraction  of  the  humin  fraction,  from  a  soil  rich  in  organic  matter  located  near  the  Pratagi River  located  in  Maceió,  AL.  Characterizations  were  carried  out  in  order  to  obtain information  on  its  structure  using  the  organic  matter  content,  atomic  ratios,  E4/E6  ratio  and Fourier-transform infrared spectroscopy - FTIR. The adsorption efficiency was determined for the  preparation  of  the  multi-element  solution  of  Ca2+  and  Mg2+  (10  mg  L-1)  at  pH  8.0.  The adsorbent  was  filtered  through  polystyrene  filters,  air  dried  and  weighed,  according  to  the amount  defined  for  application  in  the  lettuce  culture.  The  experiment  was  carried  out  in  a protected environment, cultivated in two types of soil (sandy and clayey) at UFAL – Campus Arapiraca.  The  adopted  design,  for  both  soils  used,  was  in  randomized  blocks  (DBC),  with four replications, in a 4x4 factorial scheme, with 16 treatments and 64 experimental units. The treatments were represented by: four levels of humine enriched with Ca2+  and Mg2+ (H0 = 0.0; H2 = 2.0; H4 = 4.0 and H6 = 6.0 g) and four water slides (L1 = 50 ; L2 = 75; L3 = 100 and L4 = 125%, of the culture evapotranspiration). Twelve variables were analyzed: chlorophyll index, crown  diameter,  plant  height,  fresh  shoot  weight,  dry  shoot  weight,  number  of  leaves,  leaf area, fresh root weight, dry root weight, root size, leaf water content and water use efficiency. The data were subjected to regression analysis and multivariate analysis with the following set of methods: principal component analysis (PCA) and cluster analysis in a dendrogram, using the statistical software R. In the characterization of the humine sample, it was found a content of MO = 8.60, C/H ratio = 0.185 and E4 /E6 = 1.26. According to the FTIR spectrum, bands characteristic of OH, C = C and Si-O stretches were observed for the humine sample and its respective ash. Humine showed a higher adsorption rate (78.92% and 55% for Ca2+ and Mg2+, respectively) at pH 8.0 after 120 minutes of stirring. Regarding the variables submitted to the analysis  of  variance,  for  the  sandy  soil,  only  the  plant  height  and  root  size  did  not  have  a significant  effect.  For  clayey  soil,  all  variables  were  significant.  The  amount  of  variation retained by each main component measured by the first eigenvalue and the first proportion of variance  for  the  values  in  sandy  soil  had  standard  deviations  equal  to  0.44  and  3.69,  with  a higher value on the blade in L1 and less in L3 . In clayey soils, the standard deviation values were  0.43  (eigenvalue)  and  3.62  (proportion  of  variance),  with  L2  the  largest  and  L3  the smallest. In the dendrogram, for both soils, the variables and interactions between the factors were organized into four groups of similarities. In the sandy soil, it can be seen that there was a separation between two main groups: 50 and 125% sheets (L1 and L4) forming a group and 75 and 100% sheets (L2 and L3) forming another. As for the clayey soil, all levels of humine in the L1 blade remained in the same group, thus indicating greater similarities, which was not observed  for  the  other  interactions.  The  results  obtained  show  the  influence  of  the  nutrients adsorbed in the humine and the water layers in the lettuce culture, above all in its variables of commercial interest.

9
  • SUZYANNE MORAIS FIRMINO DE MELO
  • Sequencing and evolutionary analysis of the mitochondrial genome of Syagrus coronata (Mart.) Becc. (Arecaceae)

  • Advisor : CICERO CARLOS DE SOUZA ALMEIDA
  • COMMITTEE MEMBERS :
  • CICERO CARLOS DE SOUZA ALMEIDA
  • DENNIS CRYSTIAN SILVA
  • LUIS GUSTAVO RODRIGUES SOUZA
  • Data: Jul 17, 2020


  • Show Abstract
  • Syagrus coronata (Mart.) Becc. belongs to the Arecaceae family, it is a species native to Brazil that has ecological, social and economic importance, however, genomic studies on the species are still scarce. So far, the mitochondrial genomes of only two species of the Arecaceae family, Cocos nucifera L. and Phoenix dactylifera L., have been sequenced. Genomic characterization studies in the family have the potential to provide important information for understanding the origin and evolution of species. In this perspective, the objective was to sequence the mitogenome of S. coronata using single-end reads, paired-end reads and mate-pair reads. For this, the DNA extraction of Syagrus coronata was carried out and bioinformatics analyzes were performed using software for the assembly of the mitochondrial genome, microsatellite analysis, as well as analysis of the evolution of the mitochondrial genomes of the Arecaceae family. The complete circular sequence of the mitochondrial genome of S. coronata was obtained through a combination of de novo assembly methods and extension of contigs, using paired-end reads and mate-pair reads. The mitogenome of S. coronata is 642,817 bp in length, with a total of 73 genes. The comparisons made between the mitochondrial genomes of S. coronata, C. nucifera and P. dactylifera showed many rearrangements that were mediated by repetitive DNA and also high incorporation of DNA from the chloroplast. In summary, we demonstrate that: (1) first complete mitochondrial genome for the Syagrus genus; (2) the synteny between Syagrus coronata and Cocos nucifera revealed remarkable rearrangements; (3) the gene content and sequence identity in Arecaceae mitogenomas are highly conserved; (4) the incorporated chloroplast DNA contributes to the increase in the size of the mitochondrial genomes and (5) the mitochondrial genome of Syagrus coronata resembles the other mitogenomas already sequenced in the Arecaceae family (Cocos nucifera and Phoenix dactylifera). The comparative analysis between the genomes of the Arecaceae family showed that the evolution of the genomes follows the patterns presented in other families already studied.

10
  • GRACIELE LIMA SOBRINHO
  • Complexing capacity of humines and sulfonamides for environmental applications

  • Advisor : WANDER GUSTAVO BOTERO
  • COMMITTEE MEMBERS :
  • WANDER GUSTAVO BOTERO
  • ANDRE GUSTAVO RIBEIRO MENDONCA
  • LUCIANA CAMARGO DE OLIVEIRA
  • Data: Jul 24, 2020


  • Show Abstract
  • The accumulation of pollutants in aquatic systems is a recurring problem of
    population growth and inadequate waste management. Substances capable of
    interacting with antimicrobials affecting their disposition in the environment is of great
    environmental interest. Humine has the ability to complex metals and adsorb
    contaminants. In this context, the work sought to evaluate the complexing capacity
    between humines and sulfonamides. The humine sample A (HA) was collected in an
    agricultural area with crop rotation in the municipality of Arapiraca-AL, while the
    humine sample M (HM) was collected from a non-agricultural area near the Pratagi
    River in the municipality of Maceió-AL. The humine samples were characterized by
    different analytical techniques seeking to evaluate their characteristics. For the
    complexation, a tangential flow ultrafiltration system equipped with a 1 kDa cellulose
    membrane was used, in order to separate the free sulfanilamide from the fraction
    bound to the humine. Quantification of sulfanilamide was performed using HPLC-UV.
    The complexing capacities of the humines were around 11.11 mg g -1 for HA and
    117.0 mg g -1 for HM. According to the data analyzed, the values presented show that
    HM showed an affinity 10 times higher than HA. This behavior can be explained
    according to its characterizations, because HM has an organic matter content higher
    than HA. The results indicate that Humine can directly influence the adsorption of
    contaminants present in aquatic systems, and can be used as a chelating resin in the
    complexation of these pollutants.

11
  • FERNANDA STEFANNY LIMA SOBRINHO
  • Volatile organic compounds of yellow passion fruit flowers (Passiflora edulis Sims) and catingueira (Caesalpinia pyramidalis), pollinated species by carpenter bees (Xylocopa spp.)
  • Advisor : JOÃO GOMES DA COSTA
  • COMMITTEE MEMBERS :
  • JOÃO GOMES DA COSTA
  • ADEILDO JUNIOR DE OLIVEIRA
  • EDJANE VIEIRA PIRES
  • Data: Jul 27, 2020


  • Show Abstract
  • The service provided by pollinators in agriculture is essential, especially for fruit species. Passion fruit is a good example of this dependence on the pollinating agent. In the absence of bumblebees (Xylocopa spp.), The production of passion fruit is not commercially viable and artificial pollination is necessary, consequently increasing production costs. Thus, the objective was to identify the volatile organic compounds released by the passion fruit and catingueira flowers, both pollinated by bumblebees, in order to generate knowledge for the development of future strategies for attracting and maintaining these insects in orchards. The Volatile Organic Compounds (VOCs) emitted by the flowers of the passion fruit and catingueira plants were collected directly from the plant through the dynamic hedspace system, using Porapak Q adsorbent (80/100 mesh, 0.05 g; Supelco). Air was injected into the plastic bag before passing through an activated carbon filter at a flow of 400 mL / min for each flower. Tubes with Porapak Q were used at the top of the bag and the air was collected at a flow rate of 350 mL / min for each flower. The collection was carried out with a duration of two hours, starting at 1 pm on passion fruit flowers and at 8 am on catingueira flowers. Previously, the glass tubes were washed with hexane and heated in greenhouses at 100 ° C for 60 min., for the "activation" of the adsorbent. At the end of the aerations, the desorption of each tube with Porapak Q wças carried out by passing 500 µL hexane, HPLC grade, and stored in “vials”. The identification of the compounds was done by means of gas chromatography coupled with mass spectrometry. The results obtained showed that there was no similarity in the volatiles of the two plant species, showing that bumblebees are generalist bees, therefore, they are attracted by several species from different families and by different compounds. The floral aroma of P. edulis was composed of aliphatic hydrocarbons, esters and ether. Whereas the floral headspace of C. pyramidalis was basically composed of benzene substances, which are characteristic of plants pollinated by bees. It is worth highlighting the presence of the compound 1,4- Dimethoxy-benzene and (Z) beta-ocimene in P. edulis and C. pyramidalis, respectively, described in the literature as important attractive biochemicals of bees.

12
  • MAYARA CAMILA SANTOS SILVA
  • VOLATILE ORGANIC COMPOUNDS COMMON TO THE FLOWERS OF Hancornia speciosa and Plumeria spp. BOTH POLLINATED BY SPECIES OF THE SPHINGIDAE FAMILY

  • Advisor : JOÃO GOMES DA COSTA
  • COMMITTEE MEMBERS :
  • JOÃO GOMES DA COSTA
  • RUBENS PESSOA DE BARROS
  • ADEILDO JUNIOR DE OLIVEIRA
  • Data: Jul 31, 2020


  • Show Abstract
  • The service provided by pollinators in agriculture is essential, especially for fruit species.
    Hancornia speciosa (mangabeira) is a species with great economic potential, however it is
    still exploited in an extractive manner and has a deficient natural pollination, due to a low
    presence of pollinators, mainly insects of the Sphingidae family. Studies aimed at
    understanding the functions of floral aromas of plant species that use this family as pollinators
    are essential. In this way it will be possible to establish strategies for the rational cultivation
    of mangabeira outside its natural habitat. Thus, the present work aimed to identify the volatile
    organic compounds of the flowers of mangabeira (Hancornia speciosa GOMES) and
    Jasmine-mango (Plumeria spp.), species pollinated by sphindid insects. The volatile organic
    compounds (VOCs) emitted by the flowers of the mangaba and jasmine-mango plants were
    collected directly from the plant through the dynamic headspace system, using Porapak Q
    adsorbent (80/100 mesh, 0.05 g; Supelco). Air was injected into the plastic bag before passing
    through an activated carbon filter at a flow rate of 400 mL / min. and tubes with Porapak Q
    were used at the top of the bag and the air was collected at a flow rate of 350 mL / min. for
    each flower. The collection was carried out with a duration of twelve hours starting at 17h00,
    since this time was marked by the release of a strong sweetish odor, which remains until the
    following day. Previously, the glass tubes were washed with hexane and heated in
    greenhouses at 100 ° C for 60 min, for the "activation" of the adsorbent. At the end of the
    aerations, the desorption of the volatiles in the Porapak Q was carried out by passing 500 μL
    hexane grade HPLC and stored in “vials”. The identification of the compounds was done by
    means of gas chromatography coupled with mass spectrometry. Were identified 64 volatile
    organic compounds in the floral odor of the species studied, of these, there was similarity of
    10 compounds between the two plant species, showing that they are likely responsible for
    attracting pollinators, with emphasis on linalool. Despite having been detected only in
    mangabeira, compounds such as  -farnesene,  -karyophylene and methyl salicylate have a
    chance of having action on pollinators, playing an important role in the attractiveness of
    insects.

13
  • SYMONNE PEREIRA FERNANDES
  • PRODUCTIVITY AND ACCUMULATION OF BIOMASS BY CASSAVACONSORTIUM WITH CROTALARIA-JUNCEA

  • Advisor : VALDEVAN ROSENDO DOS SANTOS
  • COMMITTEE MEMBERS :
  • VALDEVAN ROSENDO DOS SANTOS
  • MARCIO AURELIO LINS DOS SANTOS
  • ANTONIO LUCRECIO DOS SANTOS NETO
  • RUBENS PESSOA DE BARROS
  • Data: Sep 16, 2020


  • Show Abstract
  • In Brazil, cassava is widely grown in regions where agriculture is traditionally practiced as
    subsistence cultivation, one of the facts that motivates this feat is that it is easily adapted to the
    most different edaphoclimatic conditions. In Alagoas, several families are involved in the
    production and processing of cassava. However, there is little information on the accumulation
    of fresh and dry matter from the aerial part and root over the 360 DAP cycle. The study was
    conducted in two experimental areas, field: 1 - UFAL-Campus Arapiraca experimental field and
    2- producer area, (CAPA Group), being conducted from May 2018 to May 2019, with the aim of
    monitoring the accumulation of biomass and allow dividing the crop cycle into phases, defining
    the most appropriate moment for the greatest accumulation of fresh and dry mass of the aerial
    part and root, increasing the productivity and efficiency of the cultivar used. Being followed up
    to twelve months. The design was in randomized blocks in the split plot scheme; containing four
    blocks, in which the plots will comprise the presence and absence of crotalaria juncea and in the
    sub-plots the evaluation periods that were made every 30 days during the cassava cycle, with
    eight plants from each block being harvested monthly referring to the plots with and without the
    presence of the legume and the seasons. The harvested plants were separated into parts; the
    upper third (aerial part) and the roots are weighed on a precision scale and immediately
    afterwards placed in a forced ventilation oven at 65 ° C, where they remained until the weight
    stabilized. The experimental area UFAL presented for treatment without the consortium greater
    accumulation of fresh mass of the aerial part (9314 kg ha -1 ) and the root (3380 kg ha -1 ) at 360
    DAP. The treatment with the consortium showed a greater accumulation of dry mass of the aerial
    part (2,278 kg ha -1 ) and a higher rate of absolute growth at 360 DAP. The treatment without the
    consortium was superior and showed a higher accumulation of dry root mass (2005 kg ha -1 )
    followed by a higher rate of absolute growth at 360 days. And for the CAPA experimental area,
    the treatment with the consortium showed the highest accumulation of fresh mass (12850 kg ha -
    1
    ) and dry mass (4239 kg ha -1 ) of the aerial part at 360 DAP, followed by a higher absolute
    growth rate. The treatment without the consortium was the one with the highest accumulation of
    fresh root mass (18562 kg ha -1 ) and for dry root mass the treatment with the consortium (7153 kg
    ha -1 ) was the one that showed the greatest accumulation at 360 DAP. The highest rate of absolute
    growth was at 360 DAP, regardless of treatment. Yield of cassava roots did not show significant
    differences between treatments for UFAL area and for CAPA, there was a significant difference
    for treatments, where the treatment without the intercrop showed higher final root productivity.

14
  • IGOR GLEDSON DE OLIVEIRA SANTOS
  • Soil erodibility experimentally determined by simulated rain and mathematical models in Oxisols of Alagoas

  • Advisor : CICERO GOMES DOS SANTOS
  • COMMITTEE MEMBERS :
  • MARCIO AURELIO LINS DOS SANTOS
  • CICERO GOMES DOS SANTOS
  • DANIELLA PEREIRA DOS SANTOS
  • ALTANYS SILVA CALHEIROS
  • Data: Sep 17, 2020


  • Show Abstract
  • The resistance of a soil to the breakdown and transport of particles is defined as soil erodibility, which is the factor responsible for the susceptibility of the soil to water erosion, being conditioned by mineralogical, chemical, morphological, physical and biological attributes. Its determination is a complex factor and depends on the interaction of multiple attributes, to describe the behavior of the soil in the face of the erosion process. In view of this, empirical, conceptual and mathematical models were developed. This study aims at the indirect and direct determination of the erodibility of the Oxisols, in the Red, Yellow and Red-Yellow sub-regions of the State of Alagoas. The soils were identified through the modal profile in ZAAL (Agroecological Zoning ofthe State of Alagoas) and through classification surveys of companies in the sugar and alcohol sector that have identified areas of occurrence. They were subjected to chemical, physical, aggregate stability and erodibility determination by direct method, using simulated rain and indirect methods using mathematical models. Hydraulic characteristics of the flow during the tests were also studied. All the soils sampled were explored with crops, from farms with medium level of technology, such as thosecultivated with sugar cane, to those with low technologies such as those explored with subsistence crops, mainly those located in the Agreste region. The soil erodibility factor in offshoots (Ki) showed values that varied from 6.01 x 10-6Kg s m-4for the Yellow Latosol, municipality of Rio Largo and a higher value of 16.65 x 10-6Kg s m -4for the Red-Yellow Latosol in the municipality of Coruripe. This variation was also quite significant within the suborder, with the most homogeneous values being observed in the suborder of Red Oxisols. With the study, it was possible to conclude that for the soil erodibility factor for the Oxisols studied in the State of Alagoas, it is recommended to use the simulated rain methodology, in field or laboratory conditions through WEPP

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