Leishmaniasis is an endemic parasitic disease in approximately 102 countries and remains a serious public health problem. Currently, the available drugs have several pharmacotherapeutic limitations, and the search for new prototypes is urgent. In this context, a patent review of pharmacological innovations for the treatment of visceral leishmaniasis in the Americas over the last six years (2017-2023) was conducted. By inserting descriptors into platforms such as The Lens, World Intellectual Property Organization (WIPO), European Patent Office (EPO), United States Patent and Trademark Office (USPTO) and Brazilian Patent and Trademark Office (INPI) patents were found for subsequent analysis of publication year parameters, IPC, country of origin, authors, pharmacological assays and bioactives. Eleven patents report leishmanicidal activity in the Leishmania infantum chagasi species in vitro and in vivo of synthetic or natural bioactives. Furthermore, this study aims to investigate the cytotoxic and leishmanicidal activity in vitro of synthetic derivatives of purpurogallin in the species Leishmania amazonensis and Leishmania infantum chagasi. In the experimental study, the MTT colorimetric assay (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) was performed to determine the cytotoxicity of the derivatives in macrophages, followed by anti-Leishmania evaluation in promastigotes of the mentioned species and determination of the selectivity index. The results obtained demonstrated low cytotoxicity in the J774.A1 lineage (macrophages) up to the highest tested concentration (100 μM) with a maximum effect <50%, except for 19a and 18e, which exhibited cytotoxicity >80%. Substances 19a, 18b, 18d and 18e were cytotoxic against promastigotes of both species, with a maximum effect >80%. It was found that derivative 18 showed significant selectivity against L. infantum chagasi strains with a selectivity index >10,06. Therefore, efficient pharmacological innovations were observed in inhibiting the parasite causing visceral leishmaniasis in the Americas, as well as the leishmanicidal activity of purpurogallin derivatives in both tested Leishmania species and low cytotoxicity against host cells.

Protease inhibitors are substances that have the ability to regulate the action of proteases, blocking their catalytic activity. Many of these inhibitors are produced naturally by the body with the aim of maintaining homeostasis. Protease inhibitors have been the subject of study due to their biotechnological potential in pest control, the food industry, research into new medicines and also to increase understanding of inhibition mechanisms. The present work aimed to investigate the inhibitory activity and biochemical characteristics of a protease inhibitor present in the insect Tribolium castaneum. The first step was to identify in which phase of the insect's life cycle there is a greater production of the inhibitor, then to improve the purity of the sample, the extract was subjected to purification techniques, such as saline precipitation and two-phase and three-phase aqueous systems. After choosing the best technique, the sample was subjected to resistance tests to pH and temperature, the influence of metallic salts and stabilizers, and the action against the phytopathogenic fungus Amphobotrys ricini was also tested. The study revealed the presence of the inhibitor in the larval, pupa and adult stages, with emphasis on the larval stage, which demonstrated more than 40% inhibitory activity. The method that was most efficient for reducing contaminants in the sample was the three-phase aqueous system with isopropanol. The inhibitor activity remained stable at temperatures from 40 to 70ºC, but maintained its activity even at high temperatures, and also remained stable over a wide range of pHs (5-10). Furthermore, the inhibitor activity was influenced by different metal salts and different stabilizers, with BSA and Gelatin notably enhancing the inhibitory activity. Finally, a decrease in the growth rate of the phytopathogenic fungus Amphobotrys ricini was observed. These results highlight the potential use of the endogenous protease inhibitor from the insect T. castaneum, and may also contribute to the understanding of the properties of insect inhibitors and mechanisms of enzyme regulation.

Propolis is a sticky, viscous natural substance with a characteristic odor. It has antiinflammatory, antiviral, antibacterial, antiparasitic, styptic, astringent, phytoinhibitory, phytotoxic, antioxidant, immunostimulating, hepatoprotective, cytotoxic, anticancer, antiallergic, antifungal, antiprotozoal, analgesic and antinociceptive activities. Brown propolis manifests highly variable chemical profiles throughout the Brazilian territory, which also happens, consequently, with its biological activity. Propolis has its chemical profile modified as a result of a synergism of environmental factors such as sunlight, temperature, humidity, precipitation, soil fertility and salinity. Therefore, the need for continuous improvement of the propolis quality control strategy stands out, with the aim of standardizing it. This study aimed to elucidate the seasonal variation of quality parameters of greenish-brown propolis (PMUP) from the Alagoas municipality of União dos Palmares-Alagoas-Brazil. HPLC-DAD confirmed pinocembrin as the most abundant bioactive compound in PMUP. Furthermore, artepillin-C, pinobanksin, pinostrobin, galangin and chrysin constituted markers whose concentrations were relatively high in the apiceutic period. The average content of total phenolic compounds, considering all months surveyed, consisted of 81.1 ± 26.05 mg EAG/g; the average total flavonoid content, 38.45 ± 10.10 mg EQ/g. Both results are above the concentration found in other types of brown propolis from Brazil. The average concentration of total flavanones and dihydroflavonols in PMUP was 1.59 ± 0.24 mg EP/20 mg and correspond to 7.95% of flavonones and flavononols. The average IC50 over the 12 months, in the DPPH• chemical test, was 47.72 ± 13.02 µg/mL, which can be classified as good, surpassing the sequestration capacity of other Brazilian brown propolis. The broth microdilution test revealed that Gram-positive bacteria were more sensitive to PMUP than Gram-negative bacteria. The month of January 2021, in which there was a peak in the concentration of total flavonoids, was the month in which PMUP showed the greatest capacity to inhibit the growth of all strains tested. The MICs obtained were as follows: 64-256 µg/mL for S. aureus; 256-1024 µg/mL for E. faecalis; 64-128 µg/mL for E. coli; and 64-256 µg/mL for C. albicans. In all chemical tests, bimodal variation was observed, which frequently comprised two four-month cycles with a high content of active metabolites and DPPH• radical scavenging capacity. PMUP proved to be a rich source of bioactive compounds, whose range of action is comprehensive. In vitro tests reveal the pharmacological potential of opototherapic, as its results remain significant even during periods of lower metabolite content.

Population aging is a reality all over the world, but specifically in Latin America this process has been happening more noticeably since the 60s/70s of the 20th century. In addition to the demographic issue, the aging process leads to an increase in chronic illnesses, as well as greater social and economic fragility. These aspects make elderly people more vulnerable to chronic diseases, deficiencies, hospitalizations and the use of polypharmacy. Anemia Anemia is defined by the World Health Organization (WHO) as the condition in which the hemoglobin content in the blood is below normal as a result of a lack of one or more essential nutrients. Therefore, this dissertation aims to determine the prevalence of anemia and associated factors in elderly people. This is an excerpt from the I Alagoan Diagnosis on Health, Nutrition and Quality of Life of the Elderly Population, a project arising from the PPSUS Notice. This is a cross-sectional study with a cluster sample in the state of Alagoas. The research was carried out at home and involved the application of sociodemographic, health and quality of life instruments in addition to anthropometic assessment, medication use and collection of samples for biochemical analyzes that consisted of Glycemia, Fasting Glycemia, Cholesterol, Triglycerides, Iron, Ferritin , Vitamin D among others. In this research we identified 65.5% of elderly women, and the average age of the sample was 72.0 (SD=9.1) years. A prevalence of hemolytic anemia of around 15% was identified, well above the national average, which is around 9%. 82.6% used some type of medication chronically, of these 19.74% used 5 or more medications concomitantly.

This study reports the obtaining of mixed metal Eu³⁺/Sb³⁺ layered europium hydroxides (LEuH:x%Sb (with x ranging from 0 to 30%)) using the hydrothermal route. Our research reveals a detailed analysis of the chemical, structural, thermal, and morphological characteristics of this class of materials. The results revealed slight changes in the structure for antimony fractions greater than 20%, modification in thermal stability, and significant morphological modification due to the increase in the incorporation of Sb³⁺ into the LEuH-Cl host. Furthermore, we comprehensively investigated its photophysical properties, describing its unique luminescent characteristics. Additionally, results revealed increased luminescence of Eu³⁺ ions promoted by the energy transfer between Sb³⁺ →Eu³⁺. This study contributes valuable information on the structural, thermal and photophysical properties of layered hydroxide materials containing europium and antimony, offering insights into the design of functional materials of this class for use as efficient chemical sensors, catalysis systems, platforms for drug delivery and leishmaniasis therapy.

Extractive residues of natural origin are increasingly present in the development of bioplastics and improvements to already known polymers. The incorporation of active properties is an essential factor in the search for this waste, as they provide usefulness to a resource that is often discarded, offering financial profitability to the holders of this material, as disposal becomes an option. The main objective of this study was to produce membranes containing propolis extractive residue, red and green, at concentrations of 10, 25 and 50% in association with the Poly(ε-caprolactone) polymer. The method used for preparation was simple hot mixing with incorporation of the residue, followed by simple pressing. The residues were characterized by FTIR and HPLC. The membranes, in turn, were characterized by TG, SEM, FTIR, degradability and traction tests. As a result, it was noticed that the residues, according to FTIR and HPLC analyses, still contained relatively important compounds in the samples, such as many isoflavonoids with characteristics relevant in medicine. The membranes showed degradation peaks equivalent to the hours, days and weeks analyzed. The SEM result showed that, apparently, the membranes managed to be miscible, indicating some miscibility between the base polymer and the residues. The tensile test showed that although miscibility was effectively achieved in the production process, the membranes became less resistant compared to the Poly(ε-caprolactone) polymer alone. Thus, it was possible to prepare films containing PCL whose release of flavonoid compounds is facilitated, thus demonstrating the possibility of new applications, adding value to a residue from the propolis industry.

The brown and red type propolis from the mangrove region of Alagoas are evidenced in Alagoas, being the red propolis, of defined botanical origin. The study of the chemical composition of brown propolis from the region of União dos Palmares has been the subject of this study. The study of the chemical composition is being carried out through different analytical techniques and analytical methods such as fingerprint studies (fingerprint) and possible botanical origin using HPLC-DAD-UV, LC-Orbitrap-FTMS techniques, chemical assays of total flavonoids and activity antioxidant, as well as microbiological assay of Minimum Inhibitory Concentration. The research consisted of collecting brown propolis from the municipality of União dos Palmares (PMUP) for extraction from September 2019 to August 2021. An amount between 38 and 91g of in natura sample was collected from apiaries close to the municipality of União dos Palmares. The extraction method chosen for PMUP was maceration, in two cycles of 48 hours, using 15 g of PMUP and, for each cycle, 50 ml of hydroethanolic solution at 92.8 ºGL. The extracts were pooled and concentrated to obtain a crude solvent-free extract. Crude extracts 5 (January/2021) and 12 (July/2021) were stored in a freezer until the moment of total flavonoids, antioxidant activity by DPPH, microbiological assays and chromatographic profile assay. A brown propolis key marker isolation study was performed with a brown propolis sample from October 2019 that was subjected to partitioning using liquid-liquid extraction to obtain hexane, hexane-ethyl acetate (9:1), hexane-ethyl acetate (7 :3) and dichloromethane fraction. The dichloromethane phase was subjected to column chromatography on cellulose as a solid support and Methanol:H2O acidified with acetic acid (1%) to obtain fractions between 0% methanol and 100% methanol. The MeOH10%, MeOH20%, MeOH30% and MeOH45% fractions were submitted to semi-preparative HPLC-UV for the isolation of 2 main markers (pk35; pinocembrin) and (pk23; naringenin in mixture with naringenin) of brown propolis (PMUP) that were identified by LC-Orbitrap-FTMS and NMR 1H and 13C. The phenolic compounds identified by the LC-MS technique based on comparison with data from the literature were divided into benzoic acids, cinnamic acids (caffeic acid, ferulic acid), chlorogenic acid, prenylated cinnamic acids (dupranin and artepillin C), stilbene (resveratrol), coumarins and flavonoids divided into flavanonols, flavonones, flavones, flavonols, flavana-3-ol, flavana-4-ol and chalcones. Crude PMUP extracts showed total flavonoid content of 0.5 to 2% based on the quercetin pattern and high antioxidant activity with DPPH free radical scavenging percentage between 80 and 90%. The microbiological assay using Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Candida albicans, Trichophyton mentagrophytes and Microsporum canis strains showed MIC between 32 and 1024 µg/mL for MEOH20% fraction and MIC between 16 and 256 µg/mL for MEOH35% fraction.

Introduction: The α- terpineol (TPN) is a monoterpene obtained from Protium hepthaphylum. That has several pharmacological activities such as: anti-inflammatory, anti-obesity and cardioprotection on Spontaneously Hypertensive Rats (SHR) by isoproterenol model. ISO evoked metabolic disturbance on hepatocytes, stimulate fatty acids storage on vesicles inducing non-alcoholic steatosis. The aims of this study were verify the hepatoprotective effects of TPN in SHR against liver damage induced by ISO. Methods: Experimental protocols were approved by the CEUA/UFAL nº09/2015. Male rats were allocated into 5 groups and treated for 15 days (n=5): (G1= saline 0.9% P.O/d); (G2= Infarcted saline 0.9% P.O./d + ISO 85mg/kg 2x s.c); (G3= TPN 25 mg/kg P.O./d); (G4=TPN 50 mg/kg P.O./d) and (G5=TPN 75 mg/kg P.O./d). On 16º day, rats were anesthetized (Ketamine 80 mg/kg + Xylazine 4 mg/kg i.p.) and morphometric tests were performed in accordance with Scherle´s methods. Morphology tests were examined under light by stereoscopy, while lipid levels were estimated by total cholesterol and triglyceride dosages of blood serum. The results were expressed as mean ± SEM, and analyzed statistically by ANOVA one-way followed by Newman- Keuls test considered significant when * p <0.05, ** p<0.001 and *** p< 0.0001. Results: Weight of the liver (G1= 6.92 ± 0.22g/g* vs. G2= 8.67 ± 0.3g/g), (G4= 6.40 ± 0.36g/g* vs. G2= 8.67 ± 0.31g/g), (G5= 5.22 ± 0.7g/g*** vs. G2= 8.67 ± 0.31g/g) and (G3= 7.44 ± 0.6g/g vs. G5= 5.22 ± 0.7g/g), reason liver weight by body weight (G3=1.86 ± 0.05g/g* vs. G4= 2.44 ± 0.16g/g), (G3=1.86 ± 0.05g/g** vs G5= 2.64 ± 0.23g/g), (G4= 2.44 ± 0.16g/g *vs. G2= 1.68 ± 0.05g/g) and (G5= 2.64 ± 0.23g/g** vs. G2= 1.68 ± 0.05g/g) and reason liver weight by femur weight (G1= 65.7± 9.24g/g**** vs. G2= 547.4 ± 7.15g/g), (G3= 834.4 ± 9.51g/g**** vs. G2= 547.4 ± 7.15g/g Through morphological and histopathological aspects, it was observed that TPN inhibited non-alcoholic hepatic steatosis due to damage caused by induction with isoproterenol, in addition, previous treatment with TPN significantly inhibited the increase in total cholesterol levels (G1= 21,5 ± 1,0 vs. G2= 68,1 ± 1,2****), (G2= 68,1 ± 1,2****vs. G3 = 20,7 ± 1,3****), (G2= 68,1 ± 1,2**** vs. G4 = 22,6± 1,5****) and (G2= 68,1 ± 1,2****vs G5= 22,3 ± 4,6 ****) and triglyceride G1= 23,4 ± 1,6 vs. G2= 353,4 ± 23,3 ****), (G2= 353,4 ± 23,3 vs. G3 = 28,9 ± 2,8****), (G2= 353,4 ± 23,3 vs. G4 = 27,5± 1,7****) and (G2= 353,4 ± 23,3 vs G5= 16,2 ± 1,5 ****), levels at all doses tested. In addition, after the glucose tolerance curve, it was noted that there was a reversal in this curve, which may also influence glucose metabolism, however further studies are needed. Conclusion: Taken together, the results indicate that TPN has a significant hepatoprotective effect, in hypertensive and infarcted rats.

Red propolis from Alagoas is a sticky resin made from a complex mixture of biologically active substances with great biotechnological potential. Various studies have shown that red propolis extracts have a wide variety of biological activities as an antioxidant. Drug-excipient compatibility testing is one of the most fundamental phases in the pre-formulation research process. Given the above, this research aims to evaluate compatibility and thermal stability of red propolis associated with different excipients in obtaining polymeric membranes. The ethanolic extract of red propolis by maceration. Assays were carried out to quantify the content of phenolic compounds, using the Folin-Ciocalteu reagent method, and flavonoids, using the aluminum chloride method of the EEPV. The investigation of the antioxidant activity of EEPV was carried out using the DPPH method. Synthetic membranes were prepared using the solvent evaporation method. And the physical-chemical properties of thermal stability of membranes, components, and binary mixtures were analyzed by Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetric Analysis (DSC). It was estimated that the content of total phenolic compounds was 208.0 mg EAG.g-1, and the total flavonoid content was 45.3 mg EAG.g-1. The analysis of the antioxidant activities of EEPV by the DPPH method varied between 59.36% and 88.31%. The thermal profile obtained by the EEPV TGA showed 4 thermal degradation events, none of which were superimposed. The second event (onset: 82.01 °C, endset: 135.29 °C and mass loss of 12.83%). Thermogravimetric thermal analyzes (TGA) showed sodium benzoate as the most stable excipient. Second curve (onset: 514.77 °C, endset: 562.11 °C and mass loss of 44.79%). Propylene glycol was the least stable excipient. Its second thermal event (onset: 117.43 °C, endset: 147.35 °C and 82.57% mass loss). Membranes and binary mixtures were successfully obtained and presented a uniform appearance. The MX1 membrane presented 7 thermal degradation events, with the 1st overlapping with the 2nd, the 3rd with the 4th, and the 6th with the 7th. The second thermal event (onset: 54.75 °C, endset: 104.48 °C and mass loss of 15.88%). The EEPV decomposition curve by DSC showed two thermal events. The first transition process was endothermic (onset: 153.32 °C; endset: 364.35 °C; peak: 346.48 °C; enthalpy: -152.62 J/g). The EEPV + sodium benzoate binary mixture curve showed 6 thermal events. The first event was endothermic (onset: 35.12 °C; endset: 79.85 °C; peak: 61.38 °C; enthalpy: -36.04 J/g). The MX1 decomposition curve by DSC showed 4 transition processes. The first and third being endothermic, and the second and fourth exothermic. The first (onset: 126.49 °C, endset at 136.09, and enthalpy: -21.53 J/g). Thus, the red propolis ethanolic extract showed good thermal stability. And, among the excipients, sodium benzoate was the most thermally stable. In contrast, propylene glycol was the excipient with the lowest thermal stability. And all excipients were compatible with red propolis ethanolic extract. All membranes were successfully obtained, however thermogravimetric analysis (TGA) determined that MX1 has better thermal stability.

Use of medicines and psychoactive substances by UFAL students during the COVID-19 pandemic
The Pandemic caused by SARS-CoV-2 is considered the biggest public health problem faced by humanity in this century. It has been responsible for causing important changes in lifestyle, in interpersonal relationships, resulting in an increase in domestic violence, traffic accidents, unemployment, psychological problems, changes in the undue and indiscriminate consumption of licit and illicit drugs, and medication, reduction quality of life and well-being, as well as reduced productivity and schooling. Students make up a segment of the population with considerable vulnerability to the use of psychoactive substances. This topic is the subject of research, as the consumption of psychoactive substances can impact the student's academic and personal life. The objective of the study is to determine the profile of students at the Federal University of Alagoas, within the scope of Psychosocial Toxicology, regarding the use and local pattern of use of psychoactive substances (licit, illicit and medicines), considering the pandemic caused by the new coronavirus. This is a descriptive quantitative study. For the elaboration of the research, the screening instruments, AUDIT and ASSIST, were used, aimed at undergraduates, which allowed the remote application of a questionnaire. Statistical analysis was performed using the EpiInfo 7 program. Univariate analysis was performed to describe quantitative variables related to the social and educational profile. Bivariate analysis to identify the association between psychoactive substances and social and educational variables, with the chi-square test and 5% significance. 256 responses were obtained. As for the AUDIT, 68.01% of the students reported consuming alcohol, with 6.64% presenting behavior suggestive of abuse. Of those surveyed, 14.69% reported not being able to stop drinking once they started. Changes in behavior, feelings of guilt, and inability to remember facts when drinking were observed, in addition to a trend towards increased alcohol use in both sexes during the pandemic. The ASSIST pointed to an increasing trend in the use of tobacco, alcohol and marijuana derivatives, in addition to the report of significant consumption of antidepressants and anxiolytics. The use of cocaine/crack, amphetamines, inhalants, sedatives and hallucinogens was also reported. A relationship was identified between exact sciences courses and increased use of tobacco derivatives [(18.87%) p=0.0391], marijuana [(20.75%) p=0.0066], anxiolytics [ (13.21%) p=0.9946], from the human area and increased consumption of hypnotics/sedatives [(11.58%) p=0.0202] and anti-adhesive drugs [(18.95%) p=0 .0489]. Thus, the research pointed to a diversification in the use of psychoactive substances among university students and the contribution to outline a consumption profile in order to support the elaboration of internal policies aimed at undergraduates regarding the confrontation of the use of these substances in the academic environment.

Leishmaniasis is a neglected, infectious, and non-contagious disease caused by parasites of the genus Leishmania. Leishmania has two evolutionary forms, promastigote and amastigote, and two main clinical manifestations: cutaneous and visceral, with the latter being of greater concern as it can lead to the patient's death if untreated. Despite the complexity of the disease, the therapeutic arsenal remains limited, with pentavalent antimonials being the first choice and drugs like amphotericin B and pentamidine as the second choice. However, this therapy presents undesirable adverse effects, along with significant cytotoxicity and parasitic resistance. In light of this issue, the present study evaluated extracts from fungus producing secondary metabolites from Antarctica, and also conducted a patent review regarding the leishmanicidal activity of fungal metabolites. In the experimental study, the biomass of these fungi was produced, followed by solvent extraction using ethyl acetate and acetone, pigment concentration, drying, dilution, cell viability tests (MTT), and evaluation of leishmanicidal activity against the promastigote form of L. amazonensis and L. chagasi. Yeast extracts (19L15, 4L2, 2L19, NL1, and GL19) and filamentous fungus extracts (4FFLQ2, 5YP4, 2FFLQ6 acetate, 2FFLQ6 acetone, 1EMP1, 3EMP4, and 2EMP4) were produced and tested at concentrations of 100, 30, 10, 3, 1, and 0.3 µg/mL. In the cell viability assay, the maximum cytotoxic effect found for yeast extracts was 40.54 ± 3.37%; 24.37 ± 3.66%; 29.09 ± 3.57%; 6.28 ± 1.00%; 22.98 ± 1.63%, respectively. The cytotoxic effect of filamentous fungi was: 60.53 ± 2.50%; 67.33 ± 1.51%; 19.68 ± 6.62%; 19.68 ± 6.62%; 23.38 ± 7.16%; 17.30 ± 10.59%; 29.08 ± 18.80%, and NA. In the assay to evaluate leishmanicidal activity, the tested yeast extracts (for both species) and filamentous fungus extracts (for L. chagasi) were not lethal to the parasites even at the maximum tested concentration of 100 µg/mL. The extracts 2FFLQ6 acetate and 2FFLQ6 acetone exhibited a maximum effect of 74.36 ± 4.10% and 84.62 ± 5.32% against the L. amazonensis species, suggesting the need for further studies in this area. In the patent review, four patents were selected, which demonstrated activity against L. major, L. amazonensis, and L. donovani species, along with low toxicity. Despite the results, the subject is still underexplored by the academic community, and the continuation and encouragement of bioprospecting research using fungal isolates and extracts is warranted.

Leishmaniasis is still one of the most neglected diseases in the world, affecting millions of people mainly in poor and underdeveloped countries. In Brazil, leishmaniasis is a public health problem due to its ease of propagation and ability to cause epidemics. Given this scenario, pyroligneous extracts have drawn the attention of many researchers, given that their processing can contribute to the development of chemical entities with pharmacological potential. In the Technological Prospecting carried out, it was possible to observe the
formulation of innovations using pyroligneous extracts for therapeutic purposes and the application of these extracts in the treatment of pathologies such as cancer. The exercise of technological prospection is fundamental to guide the development of new technologies. In the experimental study, seven extracts from plants typical of the Brazilian flora were tested. All extracts showed leishmanicidal effects for the evolutionary form of promastigote amazonensis (100% ± 0.00) with IC 50 ranging from 0.01% (Mimosa tenuiflora) to 0.26%
(Eucalyptus), however, they showed cytotoxic effects in the order of 95.13% (Pennisetum purpureum) for macrophages of the J774.A1 strain in the MTT assay. The results obtained in the pharmacological tests associated with those presented in the Technological Prospecting indicate that studies need to be implemented in order to standardize an extraction method, thus favoring the obtainment of compounds with pharmacological
properties.

Pesticides and herbicides are intended to improve crop productivity, protecting them and preventing the proliferation of pests. Equally, the use of veterinary products is based on the search for higher yields in livestock. In this context, substances derived from the element arsenic (As), such as sodium methylarsinate (MSMA), are present in several products for veterinary and agricultural use. Considering that such products are widely used in Brazil, and other countries, the topics that clarify the risks and toxicity of these compounds have not yet been fully explored. Metabolomics is a branch of the omics sciences that seeks to understand, through the analysis of the metabolites produced in a given organism, which factors influence specific metabolic changes, whether these occur due to lifestyle habits or exposure to any xenobiotic. The metabolomics strategy includes the analysis of biological samples using equipment such as Gas Chromatography (GC) and Nuclear Magnetic Resonance (NMR). Thus, this project aimed to investigate, through an ecotoxicological study associated with metabolomics based on 1H NMR, the proportion of impact that arsenic compounds in water can provoke on aquatic species such as zebrafish (Danio rerio). The choice of this species as a model organism was due to its considerable versatility in bioassays. The toxicity study was based on the acute exposure of embryos to MSMA, with serial concentrations in order to obtain the LD50. Sampling for the metabolomic study followed the protocols available in the literature, associated with 1H NMR spectroscopy and statistical study to get the metabolic profile. Thus, the metabolomic analysis revealed 42 metabolites involved in different metabolic pathways. The biochemical profile study suggested that MSMA caused severe impacts, especially on pathways of energy metabolism, endocrine metabolism, hepatic metabolism, and oxidative stress.

A pest, in a broad sense, can be defined as the insect (or other organism) that causes direct damage to humans, their livestock, their crops, or even their possessions. The species Tribolium castaneum Herbst (Coleoptera: Tenebrionidae), stands out as one of the main pests, which, in particular, represents 9% of economic loss of stored grains in developed countries, so that, in developing countries, these losses can represent 20% worldwide. Due to its wide distribution around the globe and its characteristics, the intestine of this species can be used as a source of enzymes for studies for pest control and applications. The objective of this work was the characterization and biotechnological application of an amylase enzyme, from Tribolium castaneum larvae, in the removal of dirt from cotton fabrics. For this purpose, cotton fabrics, previously delimited and stained with starch solution at 1% commercial tomato sauce, were subjected to a washing process with enzymatic crude extract alone and in the presence of two commercial powder soaps, detergent A and detergent B. Furthermore, the crude extract was characterized in relation to parameters such as optimum temperature and thermal stability, optimum pH and behavior in the presence of some metallic ions, such as copper, zinc, calcium and the chelating agent EDTA. Spectrophotometry was used as an analytical method to prove the removal of dirt from the fabrics. It was observed that the amylase enzyme under study showed optimal relative activity over a wide pH range, namely, from 5 to 9, optimal temperature at 37°C and residual activity of more than 80% at 50°C, demonstrating, thus, thermal stability at this temperature. In the presence of some metallic ions, the highest inhibition rates, in relation to the 10mM, 1mM and 0.1mM concentrations, were related to copper sulfate (CuSO4), which comprised 95%, 89% and 86% inhibition, respectively. In relation to the potentiality of detergency, it was observed that the enzymatic extract had the ability to break starch in dirty tissues with starch solution and also demonstrated superior cleaning power, when compared to comercial detergents under study, in front of the dirty tissue commercial tomato sauce thus implying an importante ally and potential adjuvant enzyme to be incorporated in the industry of enzymatic detergents and other products for cleaning.

Therapeutic adherence can be understood as the measure by which patients follow the recommendations for their prescribed treatments, considering the actual behavior of individuals during treatment. The use of mobile technologies to monitor and provide care to individuals with health problems is a growing phenomenon that has been used to optimize therapeutic adherence in recent years. However, few applications for smartphones have been developed by health professionals, adopt different strategies focused on different domains of adherence or have been validated so far. Objectives: To develop and validate the content of a smartphone application to optimize therapeutic adherence in patients with Hypertension. Methodology: A two-phase study was developed.1) systematic review of the literature to identify and evaluate applications to optimize adherence to drug therapies and analysis of application domains and the effectiveness of their functionalities 2 ) Content validation using the DELPHI technique. Ethical aspects: This study adhered to the norms of resolution 466/2012 of the National Health Council. Results: Of the functionalities proposed by the therapeutic adherence validation instrument for the development of the application, they had an acceptable percentage, according to the evaluation of the therapeutic adherence specialists, for the reminder category with 90% the audible alarm and 80 % push notification, for the behavioral change category with 94% personal tracking, 82% external monitoring and 72% social media and the educational change category all proposed functionalities were accepted, showing a percentage of 96%, 86% and 82% for videos, podcast and explanatory texts respectively. Conclusion: The applications used in mobile devices positively provide therapeutic adherence in patients with chronic diseases such as Hypertension, using their domains and their functionalities has improved blood pressure levels, which denotes a significant value. for the development of mobile devices in the health area, emphasizing that studies are further developed and tested in this area.

Despite the great advances in antiretroviral therapy over time, to achieve success in clinical results it is necessary to effectively take the drugs, following what was prescribed by the health professional. However, for the patient to use the medication properly, all possible information must be provided, clearly, so that he understands the therapeutic plan to be followed. The pharmacist has a fundamental role in the correct use of drug, since at the time of dispensing, it is the last contact that the patient has with a health professional before starting their treatment. Thus, this study objective to verify the level of information of patients about their pharmacotherapy and implement educational pharmaceutical interventions about their knowledge about antiretroviral therapy. Therefore, a prospective interventionist quantitative study was carried out in two health services in the state of Alagoas. To verify the level of information that patients had about their antiretroviral drug therapy, was used the instrument validated by Raehl et al. (2002), and viral charge laboratory test results were used to measure adherence before and after pharmaceutical interventions. 32 patients were followed up by a clinical pharmacist during the 10 month period. From the results obtained, it was possible to observe that the level of understanding of antiretroviral information was considered insufficient for 76.50% (n=102) of patients (p<0.001). When asked about the name of the medication they used, most participants could not inform (78.43%). There was also a high rate of disagreement (87.25%) regarding the dose of the drug, and approximately half of the participants (53.92%) said they did not know if there was any type of interaction with food or if it could be administered close to meal times. However, the most knew the indication (97.06%) as well as the administration times (94.12%) of their medications. Finally, when analyzing the results of viral charge laboratory tests performed before and during pharmaceutical follow-up, it was possible to observe an increase in the number of participants with undetectable viral charge results (< 50 copies/mL) (from 56.25% to 93.75%) (n=32) in the group followed by the clinical pharmacist (p<0.01). The present study shows that the high proportion of patients with a low level of understanding reinforces the importance of the health team in transmitting information more clearly, identifying factors associated with low understanding and solving possible doubts in order to guarantee good therapeutic results. The interventions performed by the pharmacist were able to contribute to the improvement of adherence due to the reduction of the viral charge values for the followed patients. In addition, it reinforces the importance of the pharmacist's presence in the multidisciplinary team developing clinical activities, in promoting the rational use of medicament.

Resumo em inglês: Apitoxin is a substance produced by bees that has numerous biological activities, being melittin the main biologically active peptide. Among the immune response cells, macrophages stand out, which can act in pro- or anti-inflammatory responses, being important target cells for the search for substances capable of modulating the immune response. Thus, the aim of this study was to evaluate the immunomodulatory activity of apitoxin (API) and melittin (MEL) in macrophages activated by lipopolysaccharide (LPS) in vitro. For this, murine macrophages (J774.A1 cell line) were initially incubated for 48h with API or MEL to determine the maximum non-toxic concentration (CMNT) by using cell viability assays, whose results were 3.125 μg/mL and 0.625 μg/mL, respectively. To evaluate the immunomodulatory activity of the compounds, macrophages were activated in vitro with LPS (200 ng/mL) and treated with API or MEL. The immunomodulatory activity was investigated by flow cytometry immunophenotyping, with the evaluation of the expression of markers F4/80 and CD86 and parameters of relative size (FSC) and complexity/granularity (SSC). The production of pro- and anti- inflammatory cytokines was also evaluated using the Cytometric Bead Array (CBA) methodology. It was possible to observe that MEL decreased the expression of CD86, as well as the size and complexity/granularity of activated macrophages, while API increased the expression of CD86 and F4/80, and increased the parameters of size and relative cellular complexity. Furthermore, it was also detected that MEL decreased the percentage of intracellular TNF-α producing cells and the levels of IL-6 and TNF-
α cytokines in the cell culture supernatant. On the other hand, apitoxin increased the percentage of intracellular TNF-α producing cells, as well as the levels of IL-6 and TNF- α in the supernatant. There was no statistically significant difference in IL- 10 levels in both treatments, with API or MEL, when compared to the untreated cells stimulated with LPS. In conclusion, this study verified the immunostimulatory activity of API and the immunosuppressive/anti-inflammatory activity of MEL in macrophages in an inflammatory context stimulated by LPS in vitro thus contributing to the development of new bioactive agents with immunomodulatory activity.

Introduction: Medication reconciliation corresponds to the multiprofessional clinical service component of the care transition process. Through it, it becomes possible to identify and resolve potential problems and discrepancies in pharmacotherapy, being, therefore, among the most important interventions that guarantee patient safety. However, the impact of this intervention on health outcomes in outpatients is still poorly understood. Objective: To assess the influence of medication reconciliation on clinical, economic and humanistic outcomes in adult outpatients. Method: A systematic review of studies was carried out that verified the association between the proposed intervention and some clinical, economic or humanistic outcomes published until July 2021, identified by searching the Cochrane Library, Embase, LILACS, Pubmed/Medline, Scopus, CINAHL and Web of Science databases. The search strategy involved words with the terms MESH: Medication Reconciliation, Ambulatory care, Ambulatory care facilities, Primary Health Care, Community Pharmacy Services and non-MESH: Mortality, Morbidity, Drug Costs, Quality of life and Quality-adjusted life Years. Results: 6908 articles were identified, of which 28 studies were selected for full text reading. At the end of the selection process, 13 studies were included in this review. The included studies verified important outcomes and in most of them, there was an association with the proposed intervention. Its methodological designs are mostly adequate, and the general methodological quality is moderate. So, although there are a limited number of studies, there is important initial evidence that medication reconciliation performed in outpatients can affect health outcomes. Conclusion: To date, the scientific literature indicates that medication reconciliation can affect certain clinical health outcomes in outpatients, such as hospital readmissions, visits to the urgency/emergency unit, and mortality. Given the limited number of studies and the use of certain outcomes, especially those related to the economic and humanistic components – there is a need to investigate the real impact of medication reconciliation in additional studies.

Biogenic synthesis uses natural raw material to reduce macromolecules in nanoparticles and propolis is an alternative in reducing the toxicity of silver nanoparticles. Objective: The objective of this work was to produce and characterize a dental adhesive with silver nanoparticles biosynthesized with red propolis from Alagoas. Methodology: Antioxidant (DPPH method) and antimicrobial activities against Streptococcus mutans, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, by the methods of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (CBM) of the ethanolic extract of red propolis from Alagoas (EPV), of silver nanoparticles synthesized in a conventional way (NPAgC) and biosynthesized with EPV (NPAgB) and the mixture of NPAgC + EPV. Six patches with NPAgB 250 µg / mL were developed; NPAgC 250 µg / ml; EPV 250 µg / mL; EPV 300 µg / mL; NPAgC 250 µg / mL + EPV 250 and NPAgC 250 µg / mL + EPV 300. Pure adhesive (AE) and Single Bond 2 (3M / ESPE) were used as controls. AE was also tested with 24 µL of ethanol (solvent) to check the effect of the solvent. EPV and NPAgB were subjected to high performance liquid chromatography (HPLC) assays to determine and quantify propolis biomarkers that reduce silver in nanomaterials. The experimental adhesives were characterized by measurements of the degree of conversion (infrared spectroscopy (FTIR), release of nanoparticles in artificial saliva from the polymerized adhesive and antibacterial activity by the disk diffusion method (MDD). Technological research was carried out on the topic. GC data were analyzed using one-way ANOVA (α = 0.05). Results: The average content of flavonoids in the EPV was 1.56% and antioxidant activity (AA%) ranging from 3.15 to 10.53% on average. NPAgB and NPAgC were obtained in the form of a light, fine, spherical shape, blackish in color for NPAgB and silver in color for NPsAgC. Compared to NPAgC, NPAgB showed better results of bacteriostatic activity, except against Enterococcus faecalis, where a similar result was observed for both types of NPs. The bactericidal effect of NPAgB was better for S. mutans and S. epidermidis than NPAgC. NPAgB showed AA% better than NPAgC. The red propolis markers that reduce the silver molecule in nanomaterials were obtained. The degree of conversion of the adhesives varied between 76.19 and 87.57% (p> 0.05). The MDD assay showed better results for NPAgB with a diffusion radius of 3.7mm. The release test carried out in spectrometry and absorption in UV-Vis demonstrated that the adhesives do not release NPs.The microtensile test demonstrated that although the NPAgB (39.56 MPa), EPV 250 (45.85 MPa) and EPV 300 (39.77 Mpa) adhesives had significantly higher immediate bond strength than the control group (34.88 Mpa) there is no significant difference between the analyzed adhesives (p> 0.05). The predominant fracture mode test in all promotion groups was mixed fracture. Conclusion: The synthesis of a dental adhesive with silver nanoparticles biosynthesized with red propolis from Alagoas resulted in a material with antimicrobial activity and a stable matrix with the active principle immobilized within the material.

Rincoforol is an aggregation pheromone applied as bait in traps to capture Rhynchophorus palmarum. The challenge of its commercialization is in the form of packaging, which does not guarantee its integrity during the whole period of application nor modulates its release rate. Therefore, the formulation of this pheromone in a modified release device is recommended, ensuring protection against degradation and release at appropriate levels. Therefore, the present work aims to develop a modified release device of the polymer/silicate lamellar nanocomposite (PLN) type, composed of alginate and bentonite, providing protection to Rincoforol and release at appropriate levels. Films and gels were prepared through ionotropic gelation and different silicate concentrations. The structural analysis of the nanocomposite was performed through X-ray diffractometry and infrared spectroscopy. The film product was evaluated for adhesiveness, swelling index and tensile strength. DRX and FTIR elucidated the interaction between the matrix components. The adhesiveness of the films was lost according to the applied obtaining methodology. The addition of silicate to the formulation resulted in an increase in the mechanical resistance of the polymer, resulting in lower water uptake and greater resistance to traction. The matrix, until then, shows promising as a modified release device. It is also expected to complete stability studies of Rincoforol adsorbed to the matrix and release kinetics to ensure that the nanocomposite is suitable for modified release of this pheromone.

Chikungunya virus (CHIKV) causes Chikungunya fever disease that can progress to the chronic phase characterized by persistent arthralgia. There are currently no antivirals or licensed vaccines against this virus. Alagoas Red Propolis (ARP) is a natural resin that bees produce by mixing saliva and exudate from plants that have antimicrobial activities. Therefore, our objective was the chemical characterization and evaluate the antiviral activity of ARP against CHIKV in vitro. First, CHIKV was isolated from serum in the acute viremia phase of the disease, replicated in Vero E6 cells and confirmed by RT-PCR using primers for the E1 protein genomic region (882 bp). DNA sequencing and phylogenetic analysis (MEGA7 software) confirmed East/ Central/ South African (ECSA) as the CHIKV genotype. The chemical characterization of PVA was performed by high performance liquid chromatography (HPLC-DAD) and the presence of the liquiritigenin, pinobanksin, daidzein, formononetin, biochanin A and isoliquiritigenin markers were detected. The ARP cytotoxicity in Vero E6 cells was evaluated by MTT colorimetric assay and CC50 values of 200.1 μg / mL, 22.57 μg / mL and > 40,0 μg / mL was obtained for the hydroalcoholic extract (HE), hexane fraction (HF) and ethyl acetate fraction (EAF), respectively. For the antiviral activity assays, CHIKV adsorption was performed for 2h on Vero E6 cells, followed by treatment with different concentrations of PVA solutions for 72h and cell viability analysis by MTT assay. As result, a promising antiviral activity was detected and the IC50 values were 22.80 μg/mL, 4.24 μg/mL and 5.34 μg/mL for the HE, HF and EAF, respectively. In addition, high selectivity indices (SI) were obtained for the compounds (HE = 8.7, HF = 5.3 and EAF > 7.5). In order to confirm the antiviral activity, virus intracellular labeling was performed and the percentage of positive cells was detected by flow cytometry 48h after infection. As result, all tested ARP solutions showed a reduction effect higher than 50% on the percentage of infected cells. In the inactivation assay, CHIKV suspensions were first incubated with ARP for 2h, diluted and incubated for 48h, followed by the detection of the plaque-forming units (PFU). As result, a reduction in the number of PFUs was detected [4,867 PFU / mL (EH), 12,767 PFU / mL (HF) and 8,867 PFU / mL vs. 31,000 PFU / mL untreated viral control. In addition, to assess which stages of viral infection the compounds are acting on, cells were treated with ARP and their fractions for 2h before CHIKV infection, simultaneously with infection (0h), or for different times post-infection (2h, 4h and 6h). Interestingly, the inhibitory activity was detected at 2h, 4h and 6h post- infection for both HE and HF and 2h and 4h after infection for EAF. To analyze the activity of the compounds detected by HPLC-DAD against the CHIKV proteins, a molecular Docking silica assay was performed, as a result of all the compounds detected above as targets of their activities, the CHIKV E3-E2-E1 glycoproteins complex. In conclusion, the data obtained in the present study demonstrate the promising antiviral activity of ARP against CHIKV in vitro, thus contributing to the development of new therapeutic agents for this arbovirus.

Resumo em inglês: Schiff´s bases are an important class of organic compounds, that have the azomethine group which is responsible for a wide variety of biological applications including antibacterial activity. Like Schiff's bases, heterocycles such as thiazole, pyridine and indole are pharmacophores present in several pharmaceutical products, where antibacterial properties are also reported. Thus, the present work aims at the synthesis of Schiff´s bases compounds containing the heterocycles: thiazol, indole and pyridine through the condensation of aldehydes with aromatic amines. Six compounds (LQM385-LQM390) were obtained with yields greater than 70%. The structures were characterized by the Nuclear Magnetic Resonance (NMR) method of hydrogen (1H) and carbon (13C). The antibacterial activity was evaluated by the broth microdilution method against two gram-negative bacterial strains (Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853) and two gram-positive strains (Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212), the compounds presented significant to moderate activity with minimal inhibitory concentrations (MIC) of 62.5 µg/ml-500 µg/ml against most strains. The evaluation of the antibacterial activity showed that the compounds LQM385, LQM389 and LQM390 exhibited the best MIC against the S. aureus ATCC 25923 strain, being the lowest in the study including also the MIC of the compound LQM 385 against E. faecalis ATCC 29212.

Illicium verum Hook f. (Schisandraceae), known as star anise, is a native specie to Asia marketed worldwide. The herbal drug (HD), consisting of dried fruit, is used in folk medicine and the pharmaceutical industry. In its essential oil is the anethole, the major component, to which pharmacological activities are mentioned. Cutaneous mycoses are clinical manifestations caused by a group of fungi called dermatophytes that affect humans and animals. Despite the various therapeutic options, the side effects and the resistance to antifungals are a problem for treatment, thus requiring the search for new antifungal agents. Despite its extensive use in phytotherapy and commercial, there are few studies dealing with pharmacognostic analysis of I. verum, like those of his activity on dermatophytes. Thus, the aim of this work was to make the quality control of dried fruits of I. verum and evaluate the antimycotic capacity of essential oil (IVEO) on dermatophytes. For the method, a sample of star anise was acquired in a herbal shop of the city of Maceió/AL and the quality control of HD was done according to the Brazilian Pharmacopoeia 6th edition (2019), being performed: botanical identification (macro- and microscopy), determination of foreign matter, total ash, yield and chemical profile of volatiles. Purity tests were performed in triplicate and expressed by mean and standard deviation (M±SD). For antifungal activity, six clinical samples of dermatophytes were taken: Microsporum canis (1), Trichophyton rubrum (1), Trichophyton tonsurans (1), Trichophyton mentagrophytes (3). The search consisted by microdilution in broth, whose concentrations of IVEO were from 10,000 μg/mL to 78.1μg/mL and the results were expressed by the Minimum Inhibitory Concentration (MIC) and from this, that of the Minimum Fungicidal Concentration (MFC). The identity analysis showed that the samples met the macroscopic parameters of the HD. However, microscopy showed morphological differences in fruits that did not mature, indicating in them, absence of specialized structures for oil storage. The foreign matter yield (1.6%±0.32) and total ash (3.0%±0.10) were in accordance with the monograph of the specie. The percentage of the chemical marker anethole was in accordance with the monograph (>99%), however, the volatile yield was below the expected (6.25% w/v). The MIC/MFC for the samples were from 10,000 to 78.1μg/mL (MIC) and 10,000 to 625 ± μg/mL (MFC). The results of this search show that HD met the quality requirements proposed by the pharmacopoeia and ratifies the potential and activity of IVEO in fungi due to anethole.

The term "propolis" is meant for resinous material composed of a set of natural substances, which are not collected directly from different parts and exudates from plants where salivary sections of bees, wax and pollen are added for training of the final product. Some phytochemical investigations using Alagoas red propolis (PVA) allow the identification of several classes of compounds in their composition, but are not yet well known in their volatile composition. To this end, the project aims to extract essential oil from red propolis (OEPV), physically and chemically characterize a chemical composition of extracts, ethanolic extract of red propolis (EEPV), ethanolic extract of red propolis sludge (EEBPV) Red Propolis Aqueous (EAPV) and OEPV from the state of Alagoas, evaluated as their biological activities. It performed the extraction of OEPV by hydrodistillation of red propolis, in its crude form, and the preparation of extracts (EEPV, EEBPV and EAPV), besides evaluating the quality of propolis comparing the results of the extracts against the essential oil. As the methodologies used were the physicochemical research, ash, collected, flavonoids and total phenolics), antioxidant activity, chromatographic and phytochemical profile analysis by GC-MS (Mass Spectrometry Gas Chromatography), Headspace extraction and UPLC- DAD (Ultra Performance Liquid Chromatography coupled to a Diodes Array Detector) and evaluation of antibacterial activity against Staphlococcus aureus ATCC 25923, Staphlococcus epidermidis CCCD-S010, Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 14942. It was observed in the results found in the physicochemical analysis that the EEPV, EEBPV, EAPV and OEPV, are within the standards recommended by the ABPM, being found respective values of 216.1, 272.93, 188.37 and 713.95 mg. EAG.g-1 for phenols and 25.3, 20.1 and 27.3 mg EQ.g-1 for total flavonoids, however OEPV showed no flavonoid concentration. The antioxidant activity of the extracts showed IC50 values of 11.88 μg/mL for EEPV, 13.81 μg/mL for EEBPV, 18.81 μg/mL for EAPV and 64.20 μg/mL for OEPV. GC-MS and Headspace analyzes showed the presence of major phenolic compounds such as anethole and metileugenol. UPLC-DAD showed the presence of liquiritigenin, isoliquiritigenin, formononetin and bolusanthol in EEPV, EEBPV and EAPV. In the bacterial analysis the EEPV and EEBPV extracts showed no activity against the strains tested, while the EAPV and OEPV presented activities and respective values against S. aureus (MIC 250 and 4500 μg/mL), S. epidermidis (MIC 500 and 2250 μg/mL), P. aeruginosa (MIC 1000 and 4500 μg/mL) and E. coli (MIC 500 and 4500 μg/mL). Thus, it was possible to conclude that the unprecedented results are promising and offer a basis to broaden the scope of the research with red propolis, observing in the extracts physicochemical characteristics, chromatographic and phytochemical profiles, with characteristics of a good quality red propolis.

Periodontal diseases are pathologies that cause progressive destruction of supporting tissues and dental protection in susceptible individuals. Several chemical agents have been used as adjuvants to inhibit the formation of oral biofilm and prevent the progression of periodontal disease. This study radiographically evaluated the action of Brazilian Red Propolis (BRP) in the progression of ligature-induced periodontal disease in rats. The ethanolic extract of BRP from Alagoas State was characterized by UPLC-DAD and tested for cytotoxicity in J774.A1 macrophages. Forty-two Wistar rats, divided into groups (n = 6): Group C (without treatment); Group G (Gel - pure natrosol gel application); CHX Group (Chlorhexidine - 2% chlorhexidine gel); Group PV10 (BRP gel at 10 mg / ml); Group PV15 (BRP gel at 15 mg / ml); Group PV10 + CHX (BRP 10 mg / ml + 2% chlorhexidine gel); Group PV15 + CHX (BRP 15 mg / ml + 2% chlorhexidine gel) were used in the study. The gels were applied daily, since the insertion of the ligature, for a period of 15 days. After the experimental period, the animals were euthanized and radiographic analyzes were performed using cone beam computed tomography to measure periodontal bone support. The values obtained were analyzed by variance (ANOVA) followed by Tukey's post-hoc. The results obtained indicated the presence of anti-inflammatory and immunomodulatory flavonoids, such as liquiritigenin, daidzein, formononetin, isoliquiritigenin, pinobanksin, biochanin A and pinocembrin. The cytotoxicity test demonstrated BRP was non-toxic in concentrations up to 10 µg / ml. The tomographic evaluation showed significantly less bone loss (p <0.05) in the groups that used BRP in relation to the control and vehicle, whereas there was no statistically significant difference between the experimental groups. In conclusion, the application of gel with BRP from Alagoas demonstrated a protective effect against bone resorption due to experimental periodontitis similar to chlorhexidine, being a potential therapeutic agent in the management of periodontal disease.