|
Thèses |
|
1
|
-
IARA MARIA DOS SANTOS COSTA
-
GENETIC DIVERSITY OF BEAN BEAN-ASSOCIATED BEGOMOVIRUS CULTIVATED IN A FAMILY PRODUCTION SYSTEM IN ALAGOAS
-
Leader : SARAH JACQUELINE CAVALCANTI DA SILVA
-
MEMBRES DE LA BANQUE :
-
GILDEMBERG AMORIM LEAL JUNIOR
-
MAYRA MACHADO DE MEDEIROS FERRO
-
ROBERTO RAMOS SOBRINHO
-
SARAH JACQUELINE CAVALCANTI DA SILVA
-
Data: 16 févr. 2023
-
-
Afficher le Résumé
-
Beans are a staple food of fundamental importance for food and nutritional security as a source of protein, in addition to income for small producers in Alagoas. Practically all of the bean production in the State of Alagoas comes from family farming, which it uses for its own consumption and sells its surplus, supplying the demands of the domestic market. Production is based on the cultivation of common bean (P. vulgaris) and broad bean (P. lunatus), and to a lesser extent, cowpea (Vigna unguiculata) and pigeonpea (Cajanus cajan). The productivity of these crops is considered low, mainly due to the low adoption of technology and the occurrence of phytosanitary problems, with emphasis on viruses that belong to the genus Begomovirus (Family Geminiviridae). These have limited production and reduced grain quality in bean crops grown in all areas of Brazil. Several begomoviruses have been reported naturally infecting cultivated and non-cultivated legumes, but Bean golden mosaic virus (BGMV) and Macroptilium yellow spot virus (MaYSV) are the most economically important in the country. These species occur in Alagoas since 2005 in bean and broad bean crops, and non-cultivated plants such as Macroptilium spp., with MaYSV being the prevalent virus. In this context, the objective of this research is to determine the genetic diversity of begomoviruses associated with P. lunatus in a family production system in Alagoas. Leaf samples of P. lunatuss were collected in different areas of family cultivation in the state of Alagoas. The total DNA of each sample was extracted and used, initially, as a template for detection of begomoviruses, using degenerate primers. Subsequently, the detection of the genomic components DNA-A and DNA-A of BGMV and MaYSV was performed using species-specific primers. Samples that tested negative for BGMV and MaYSV begomoviruses had their genomic components amplified via RCA, cleaved with restriction enzymes, ligated into the pBluescript KS+ vector and cloned into Escherichia coli DH10B. Clones containing recombinant vectors were selected for primer walking sequencing. 2 DNA-B clones were obtained and subjected to whole-genome pairwise analysis with otherpreviously reported begomoviruses. These were more closely related to the species BGMV BR:Pai8:11 from Palmeira dos Índios - AL, with 95.72% nucleotide identity. However, it was not possible to correctly attribute taxonomy to the new isolates, due to the absence of DNA-A. The phylogenetic relationship between the characterized isolates was determined by reconstructing Bayesian inference phylogenetic trees for the DNA-A and DNA-B genomic component dataset. Possible recombination events and sites between the begomoviruses found and others stored in databases will also be determined.
|
|
2
|
-
JESSICA MARIANA SILVA COSTA
-
SOURSOUP SEED EMULSIONABLE EXTRACT: POTENTIALITY FOR THE CONTROL OF PLANOCOCCUS CITRI (RISSO, 1813) (HEMIPTERA: PSEUDOCOCCIDAE) IN THE FIELD
-
Leader : ROSEANE CRISTINA PREDES TRINDADE
-
MEMBRES DE LA BANQUE :
-
ROSEANE CRISTINA PREDES TRINDADE
-
MARIANA OLIVEIRA BREDA
-
JOSE DAILSON SILVA DE OLIVEIRA
-
Data: 23 févr. 2023
-
-
Afficher le Résumé
-
The mealybug, Planococcus citri (RISSO, 1813) (Hemiptera: Coccidae), has a range of hosts, attacking several crops of agricultural and ornamental importance. It is a pest that is found in tropical, subtropical and temperate regions and for its control, there are few insecticides registered for all affected crops. For sugar apple, Annona squamosa L. (Annonaceae), control methods are non-existent. Thus, research focused on control strategies, including applicability in the field with plant extracts, is a very promising area because it has the advantage of not causing damage to humans, the environment, non-target organisms and not selecting resistant populations. Plant extracts, from species of the Anonnaceae family, have been shown in several studies to be a promising alternative for the control of agricultural pests, including showing selectivity to natural enemies. Thus, the objective of this work was to evaluate the toxicity of the emulsifiable extract of soursop seeds, Annona muricata L. (Annnaceae), on the mealy cochineal, in sugar apple fruits, Annona squamosa L. (Anonnaceae), under laboratory and field conditions. with the Verdinha and Crioula varieties. For that, the experiments were carried out in the sugar apple orchard, Annona squamosa L. (Annonaceae), in the experimental area of the Campus of Engineering and Agricultural Sciences of the Federal University of Alagoas. For the formulation of the emulsifiable extract, different amounts of Span® and Tween®, distilled water and ethanol extract of soursop were used. For lethal and residual effect tests, adult females of P. citri were used. Through contact, the lethal concentrations LC50 and LC99 (0.8% and 2.6%) were estimated, using only the LC99 to evaluate the insecticidal action of the emulsifiable extract. The toxicity of the extract in the laboratory caused mortality of 94.71%, while in the field in the Verdinha and Crioula varieties it was 100% and 97.03%, respectively. The residual effect in the laboratory was more effective with 2 hours of exposure with a mortality of 61.39% at the end of the evaluation. For the Verdinha variety, the best exposure time was 24 hours with a mortality of 87.03%, for the Crioula variety the extract was more promising with 48 hours causing a mortality of 70.76%. The emulsifiable formulation has a lethal and residual effect on adult P.citri females. The morphological characteristic of the Verdinha variety provided an increase in the efficiency of the extract on mealybugs.
|
|
3
|
-
MARIA ISABEL GOMES DOS SANTOS
-
RESISTANCE OF YELLOW PASSION FRUIT (Passiflora edulis) GENOTYPES TO ANTHRACNOSE
-
Leader : GILDEMBERG AMORIM LEAL JUNIOR
-
MEMBRES DE LA BANQUE :
-
GILDEMBERG AMORIM LEAL JUNIOR
-
JAQUELINE FIGUEREDO DE OLIVEIRA COSTA
-
SARAH JACQUELINE CAVALCANTI DA SILVA
-
Data: 27 févr. 2023
-
-
Afficher le Résumé
-
The genus Passiflora (family Passifloraceae) occurs mainly in tropical regions. Brazil is considered the most important center of diversity of the genus and the largest producer of passion fruit. Phytosanitary problems limit the expansion of the crop and the maintenance of crops in the same area for a prolonged period. Anthracnose is one of the fungal diseases that threaten the passion fruit production sector. Caused by fungal species of the genus Colletotrichum spp. and in the passion fruit crop, genetic resistance is the best alternative for disease control. However, there is a need to identify the sources of resistance and characterize the accessions for indication in crosses for the development of new varieties. The research aimed to identify accessions of yellow passion fruit (Passiflora edulis) with resistance to anthracnose in the germplasm bank of EMBRAPA Mandioca e Fruticultura Tropical. Colletotrichum spp. were isolated from plants with symptoms of anthracnose in crops from Alagoas and Bahia. The isolates were evaluated for aggressiveness on passion fruit cultivated by farmers. Four isolates were inoculated into 16 passion fruit accessions. When the plants reached 60 days after germination, with 5 to 6 leaves, they were inoculated with a mycelium disc in a lesion made with a sterilized needle on the adaxial surface. The incidence of anthracnose was determined based on the appearance of the first lesions, measuring the diameter of the lesion, the first reading being taken 2 days after inoculation. The collected data were submitted to analysis of variance (ANOVA), and the averages were compared using the Scott-Knott test, at a 5% significance level, using the SISVAR program. Questionnaires were applied to passion fruit producers in Alagoas, in order to analyze the real situation they face for the production and marketing of passion fruit. It was possible to see that family farming in Alagoas is strong and that passion fruit is a well-regarded product for commercialization. However, for producers, improvements are needed in the context in which they live, such as training courses and education in the field of quality. The Gigante Amarelo and HRI30 P.A genotypes were considered less susceptible and more susceptible, respectively, to anthracnose, taking into account that the Gigante Amarelo is an improved variety released as resistant in the market, being one of the most used by passion fruit producers in Alagoas.
|
|
4
|
-
ROSINEIDE DOS SANTOS COSTA
-
Resistance of banana cultivars Musa spp. (Zingiberales: Musaceae) to the mite Raoiella indica Hirst (Acari: Tenuipalpidae)
-
Leader : EDMILSON SANTOS SILVA
-
MEMBRES DE LA BANQUE :
-
EDMILSON SANTOS SILVA
-
ELIO CESAR GUZZO
-
MARCIA DANIELA DOS SANTOS
-
MARIA DE FATIMA SILVA MUNIZ
-
MÉRCIA ELIAS DUARTE
-
Data: 28 févr. 2023
-
-
Afficher le Résumé
-
The mite Raoiella indica Hirst, 1924 (Acari: Tenuipalpidae) was originally reported in Brazil in 2009 causing negative impacts on several plant species, among the most important are the families Arecaceae and Musaceae. In Brazil, there are few studies investigating banana tree varieties resistant to the red palm mite. Thus, the present study aims to test six banana cultivars to determine the survival of R. indica and to verify the possible resistance of the cultivars to this mite. At the Laboratory of Entomology and Acarology /LEA at UFAL, Campus Arapiraca, leaf discs of six banana cultivars ('Grand Naine', 'Maçã', 'Prata Anã', 'Prata Catarina' 'Prata Gorutuba' and 'Thap Maeo') (Musa sp.) and coconut Cocos nucifera L. (Arecaceae) (control) were infested with five couples of R. indica and kept in B.O.D, at 25 ± 2 ºC, 57% ± 5 RH and 12h photophase. The evaluations took place daily with verification of the number of live mites and deposited eggs, number of larvae, nymphs and emerged adults. With these data it was possible to calculate the average survival of adults and of females and males separately, in addition to egg viability and survival of immature forms. The design was completely randomized with seven treatments (six banana cultivars + coconut as control) and 10 replications. The results were subjected to analysis of variance and their means compared by the Scott-Knott test using the statistical program AgroEstat ver. 1.1. The maximum longevity of R. indica was observed in the coconut tree at 44 days and at 41 days in the banana cultivar 'Prata Catarina'. The lowest maximum longevity rates were observed in 'Grand Naine' (26 days) and 'Prata Anã' (33 days). Regarding the sex of the mite, regardless of the evaluated cultivar, the females had greater longevity and survived until the 44th day of evaluation. Total oviposition was higher in the control (13.16 eggs) and in 'Thap Maeo' (1.86 eggs), but the latter did not differ from 'Maçã' (1.74%). The lowest oviposition averages occurred in 'Prata Anã' (0.43%) and 'Grand Naine' (0.05%), differing between themselves and between the other banana cultivars and the control. The oviposition period ranged from 21.9 days in the coconut tree to 2.1 days in the 'Grand Naine' cultivar. Considering the viability of the entire development period (egg-larvae-nymph-adult) of R. indica, there was a significant difference between the coconut palm and among all banana cultivars. The survival of the R. indica mite was higher in the 'Prata Catarina' cultivar and in the 'Thap Maeo' cultivar there was the longest oviposition period and the highest average number of eggs. In the 'Grand 'Naine' and 'Maçã' cultivars, the immature forms did not reach the adult stage. The 'Grand Naine' cultivar can be considered resistant to R. indica attack.
|
|
5
|
-
GESSICA JACIRA TRINDADE DE SOUZA
-
SPECIES OF COLLETOTRICHUM ASSOCIATED WITH BANANA IN THE STATE OF ALAGOAS
-
Leader : IRAILDES PEREIRA ASSUNCAO
-
MEMBRES DE LA BANQUE :
-
IRAILDES PEREIRA ASSUNCAO
-
GILDEMBERG AMORIM LEAL JUNIOR
-
MARIA DE FATIMA SILVA MUNIZ
-
MARIOTE DOS SANTOS BRITO NETTO
-
Data: 28 févr. 2023
-
-
Afficher le Résumé
-
Brazil annually produces about 6.7 million tons of bananas, the cultivation of banana trees (Musa spp) in the country moved R$ 9.9 million reais in the year 2021, being established, therefore, as an activity of great social importance and economic for the country. The northeast region represents 40% of the Brazilian areas cultivated with bananas and the State of Alagoas plays an active part in this production. However, the average yield of banana production in Alagoas is two tons below the national range, data resulting from limitations generated by diseases in the post-harvest of fruits, such as anthracnose, which is caused by phytopathogens of the genus Colletotrichum, whose infection it is characterized by small lesions that coalesce and form large necrotic areas in the fruit's epidermis, reducing their commercial shelf life by stimulating the early ripening of the fruit, generating losses of up to 40% of production. Therefore, in view of the relevance of investigations regarding the Colletotrichum species associated with banana anthracnose, this study aimed to characterize the distribution, prevalence and resistance of Colletotrichum isolates in the State of Alagoas, based on physiological, morphocultural, pathogenic characteristics and molecular properties of these species. The results obtained here indicate that banana anthracnose in the state of Alagoas is associated with the species Colletotrichum siamense and Colletotrichum musae¸ and the fungicide difenoconazole inhibits up to 85% of the mycelial growth of C.musae.
|
|
6
|
-
GESSIANE MARIA DA SILVA SANTOS
-
COMPONENTS OF SAPROPHYIC ADAPTABILITY OF ISOLATES OF Rhizoctonia solani AG-4 HGI ASSOCIATED WITH BRASSICA HORTALITISTS IN BRAZIL
-
Leader : KAMILA CÂMARA CORREIA
-
MEMBRES DE LA BANQUE :
-
IRAILDES PEREIRA ASSUNCAO
-
KAMILA CÂMARA CORREIA
-
MARISSONIA DE ARAUJO NORONHA
-
Data: 8 mars 2023
-
-
Afficher le Résumé
-
Rhizoctoniosis is an important disease of vegetable brassicas in Brazil, caused mainly by Rhizoctonia solani AG-4 HGI. Still, so far, there are no studies on the saprophytic adaptability components of the isolates. Therefore, the objective of this dissertation was to compare the saprophytic adaptability of the isolates of R. solani AG-4 HGI associated with vegetable brassicas in Brazil concerning the sensitivity of mycelial growth to different temperatures, pH levels, water potential and salinity, and fungicides. In all experiments, 42 isolates of R. solani obtained from vegetable brassicas with rhizoctoniosis symptoms and belonging to the AG-4 HGI anastomosis group were used. The isolates differed in sensitivity at temperatures of 15, 25 and 35 °C, as well as at pH levels 5, 7, and 9, water potential of -0.8, -1.6, and -3.2 MPa and salinity of 1, 2, and 4%. Most isolates showed greater growth at 25 °C and less at 15 °C. Using Scott-Knott cluster analysis, five groups of isolates were found at 15°C and 25°C, and eight groups at 35°C. Most isolates showed lower growth at pH 9, but 38.1% of isolates were not affected by pH. Six, five, and four groups of isolates were found at pH 5, 7, and 9, respectively. All isolates reduced the growth rate with decreasing water potential, and eight groups of isolates were found at -0.8 MPa and -3.2 MPa, and seven groups at -1.6 MPa. Most isolates reduced growth with increasing salinity level, with five groups of isolates found at 1% and 2% salinity, and four groups at 4% salinity. Most isolates showed sensitivity to fludioxonil, pencycuron and methyl thiophanate fungicides, but with different levels of growth inhibition. Means of inhibition were 99.94%, 91.59% and 12.01% for fludioxonil, pencycuron and methyl thiophanate, respectively. The growth inhibition of 54.8% of the isolates was high and similar with fludioxonil and pencycuron. Only one group of isolates was found when fludioxonil was used, two groups with pencycuron and 11 groups with methyl thiophanate. Multivariate analysis separated the 42 isolates into five groups. The R. solani AG-4 HGI isolates, despite belonging to the same anastomosis group, are separated into different similarity groups based on saprophytic adaptability, indicating the need for this variability to be considered in the development of management strategies for rhizoctoniosis in vegetable brassicas in Brazil.
|
|
7
|
-
RAFAEL DE ALMEIDA LEITE
-
SUSCEPTIBILITY OF DWARF COCONUT PALM CULTIVARS TO THE RED PALM MITE.
-
Leader : ELIO CESAR GUZZO
-
MEMBRES DE LA BANQUE :
-
ELIO CESAR GUZZO
-
EDMILSON SANTOS SILVA
-
MARIANA OLIVEIRA BREDA
-
ANIBAL RAMADAN OLIVEIRA
-
ADENIR VIEIRA TEODORO
-
Data: 28 juin 2023
-
-
Afficher le Résumé
-
The red palm mite Raoiella indica Hirst, 1924 (Acari: Tenuipalpidae) is an important pest of the coconut palm Cocos nucifera L. (Arecaceae) and has caused problems in coconut production due to its ease of dispersion and insufficient registered products to control it. Research has continued since its arrival on the American continent, aiming to control the mite through chemical, biological, alternative methods and through resistant plants. Resistant plants are a great alternative to reduce problems caused by pests. Therefore, the objective of this work was to evaluate the performance of R. indica in six dwarf coconut cultivars, in order to identify sources of resistance of these cultivars to the mite. The performance of R. indica was evaluated on the cultivars Green Dwarf from Brazil from Jiqui (AVeBrJ), Red Dwarf from Cameroon (AVC), Red Dwarf from Malaysia (AVM), Red Dwarf from Gramame (AVG), Yellow Dwarf from Gramame (AAG) and Yellow Dwarf from Malaysia (AAM), through confinement and free-choice tests, evaluating longevity, pre-oviposition and oviposition periods, fertility, fecundity, viability of life stages and choice for feeding and oviposition. The AVeBrJ cultivar was the most resistant to R. indica, providing the lowest adult survival, oviposition period, number of eggs, egg viability, fecundity and fertility, in addition to being less preferred in relation to AVM and AAM in the free-choice test. The resistance of this cultivar is probably related to some chemical factor.
|
|
8
|
-
LILIAN RENATA ALVES FARIAS
-
ECPROTOCOL FOR TOXICOLOGICAL ANALYSIS OF BOTANICAL INSECTICIDES: EFFECTS OF THE MICROENCAPSULATED FORMULATION OF THE ETHANOL EXTRACT OF Annona muricata L. (ANNONACEAE) ON NILE TILAPIA
-
Leader : ROSEANE CRISTINA PREDES TRINDADE
-
MEMBRES DE LA BANQUE :
-
ROSEANE CRISTINA PREDES TRINDADE
-
MARIANA OLIVEIRA BREDA
-
JERUSA MARIA DE OLIVEIRA AMORIM
-
Data: 9 août 2023
-
-
Afficher le Résumé
-
Toxicological studies have become more and more frequent due to the growing concern about the effects on the environment and on non-target organisms. This increased attention is also related to the increased adoption of alternatives, such as the use of botanical insecticides in agriculture. This concern is accentuated by the absence of specific legislation that establishes parameters for the use of these products, which implies the lack of defined toxicological limits. In view of this, the objective was to evaluate possible toxic effects of the microencapsulated formulation of ethanolic extract of Annona muricata L (Annonaceae), a product with a patent already granted (BR102018008313-9), on fingerlings of Nile tilapia, Oreochromis niloticus (Linnaeus, 1758) (Cichliformes: Cichlidae). Acute and chronic toxicity tests were performed, with tilapia fingerlings approximately 30 days old with measuring on average between 1.5 and 3.0 cm, which were exposed to concentrations to increasing concentrations 0.125; 0.250; 0.500; 1.0 and 2.0 g/L of the formulation for 24 and 96 hours. As well as biochemical parameters with antioxidant enzymes. Based on the mortality results, the concentration values that kill 50% of the evaluated population (CL50), obtained by Probit analysis by SAS, resulting in 0.229 and 0.249 g/ L in 24 and 96 hours, respectively. The enzymatic activity of superoxide dismutase (SOD) and glutathione S-transferase (GST) was quantified in the liver and gills and acetylcholinesterase (AChE) was quantified in the brain of O. niloticus. ANOVA statistical analysis showed differences only for SOD and GST activities in the liver. Despite that, when individuals were exposed to the two highest concentrations 1.0 and 2.0 g/L, they showed mortality rates of 93.33 and 100%, respectively, after 24 hours of exposure. Mortality likely stemmed from disturbance of the animal and alterations to its natural habitat. This is because the results of enzymatic activity and Acetyl analysis were positive when compared to the estimated LC50 value. Therefore, since toxicology is a weakness in the promotion of alternative products, more studies are needed in the area of natural products, such as extracts and oils.
|
|
9
|
-
THAYNNARA PAULA DOS SANTOS LIRA
-
PROSPECTING RESISTANCE TO Colletotrichum truncatum IN THE GENETIC DIVERSITY OF THE CREOLE VARIETY OF Phaseolus lunatus
-
Leader : GILDEMBERG AMORIM LEAL JUNIOR
-
MEMBRES DE LA BANQUE :
-
GILDEMBERG AMORIM LEAL JUNIOR
-
SARAH JACQUELINE CAVALCANTI DA SILVA
-
MARIA DE FATIMA SILVA MUNIZ
-
Data: 18 août 2023
-
-
Afficher le Résumé
-
The cultivation of Fava bean (Phaseolus lunatus L.) is typically conducted by family farmers and represents a crop of significant economic value in the Northeast region. However, substantial losses in the crop have been common due to the action of phytopathogenic fungi. Fungi of the Colletotrichum genus cause anthracnose, a disease that affects various plant species, including Fava bean. Genetic resistance presents itself as a sustainable and low-cost alternative for disease control. However, there is a need to identify sources of resistance and characterize them in order to perform crosses and develop new varieties. The research aimed to identify the ethnovarieties of Fava bean present in the municipality of São Sebastião/AL, ascertain whether the producers possess ancestral knowledge and emotional connections with the cultivated varieties, and evaluate resistance to anthracnose using the Colletotrichum truncatum (ICT12) isolate. Predefined questionnaires were used to interview Fava bean producers in the region to record cultivation conditions and the history of collected materials. Thirty-seven seed samples were acquired, identified, and stored at the Phytopathological Clinic of the Campus of Engineering and Agricultural Sciences (CECA) of UFAL. The varieties were subjected to pathogenicity tests in a controlled environment and on detached leaves through inoculation of the isolate on the upper surface of the leaves. Among the interviewed producers, the majority (45.4%) had over 30 years of cultivation experience. Additionally, 63.3% stated that they obtained the seeds from their ancestors. Regarding cultivation practices, 54.4% of the producers usually intercrop Fava beans with corn and store the seeds in plastic bottles (PET). The use of fertilizer is the primary management method for 90.9% of the respondents. Agriculture is the sole source of income for 81.8% of them, and they do not require assistance in cultivating Fava beans. In terms of consumption and commercialization, 63.6% of the producers stated that they consume the Fava beans they produce, while 36.3% not only consume but also sell them. In the in vivo and in vitro evaluations, the PLI and PLGB varieties showed high resistance, while the PLLS5 variety demonstrated high susceptibility. The producers possess ancestral knowledge and emotional connections with the cultivated varieties of Fava bean, which show great promise for studies on genetic resistance to pathogens.
|
|
|
Thèses |
|
1
|
-
EDIVÂNIA MATIAS DA SILVA
-
LETHAL AND SUBLETAL EFFECTS OF NAPHTOQUINONE ANALOGS AGAINST Mononychellus tanajoa (Bondar, 1938) (ACARI: TETRANYCHIDAE), PEST PRESENT IN CASSU (Manihot esculenta Crantz).
-
Leader : EDMILSON SANTOS SILVA
-
MEMBRES DE LA BANQUE :
-
ADEILDO JUNIOR DE OLIVEIRA
-
EDMILSON SANTOS SILVA
-
JANDIR CRUZ SANTOS
-
MÉRCIA ELIAS DUARTE
-
ROSEANE CRISTINA PREDES TRINDADE
-
Data: 16 févr. 2023
-
-
Afficher le Résumé
-
The cassava green mite, Mononychellus tanajoa (Bondar 1938) (Acari: Tetranychidae), is of great importance and can cause considerable losses in cassava crops. The control of the green mite, M. tanajoa is difficult, since until now there are no acaricides registered for this pest in the cassava crop. Natural and synthetic naphthoquinone compounds stand out for having varied biological activities, including acaricides. Therefore, the present work aimed to analyze the lethal and sublethal effects related to the repellent effect, instantaneous population growth rate and residual effect of natural and synthetic naphthoquinone compounds on the green mite, M. tanajoa in the cassava crop. Lethal concentrations of naphthoquinones were estimated by Probit analysis. To evaluate the repellent effect, cassava leaf discs were sprayed with water + 2%DMSO (control) or with the treatments (CLs 5, 25, 50, 75, 90 and 95) of the nine naphthoquinone compounds. Then, they were placed in a Petri dish and connected by a coverslip, with the treated leaf disc on one side and the control on the other. In the center of the coverslip 10 females of M. tanajoa were released and after 24 hours the number of females in each disc was evaluated, 15 repetitions were performed per treatment. For the instantaneous population growth rate (ri), cassava leaf discs were immersed with the compounds (CLs 5, 25, 50, 75, 90 and 95) and the control. Five females were transferred per disc and ten replicates were used per treatment. After 10 days, the total number of mites was counted. To evaluate the residual effect, cassava seedlings were sprayed with the LC95 of the compounds. After 2 h of application and during the periods of 1, 2, 4, 8 and 16 days after application, leaf discs were removed, placed in Petri dishes and transferred 10 females of M. tanajoa, the mortality assessment was 72 h after the application. lockdown. Among the naphthoquinones, the derivative 2-(4-((5-Methyloxazol-3-yl)-methylsulfonyl)-phenylamino)-naphthalene-1,4-dione) (C7) was the most toxic, followed by ((4 -Pyridin-3-yl)-methylsulfonyl)-phenylamino)-naphthalene-1,4-dione (C6), and by 2-bromo-naphthalene-1,4-dione (C4), with LC50 values (IC 95%) of 3.08; 5.29 and 6.33 µL/mL and LC90 (95% CI) 16.21; 24.53 and 34.31 µL/mL, respectively. Therefore, the following order of acaricidal activity was observed against the green mite, M. Tanajoa C7 > C6 > C4 > C2 > C3 > C1 > C8 > C9 > C5 for both LC50 and LC90. The viability of M. tanajoa eggs was affected after the application of Naphthoquinones (C1-C9). Based on LC50 and LC90 values 2-(4-((5-Methyloxazol-3-yl)-methylsulfonyl)-phenylamino)-naphthalene-1,4-dione) (C7) was the most toxic, followed by ( (4-Pyridin-3-yl)-methylsulfonyl)-phenylamino)-naphthalene-1,4-dione (C6), 2-bromo-naphthalene-1,4-dione (C4) and by (N'-(1, 4-Dihydro-1,4-dioxonaphthalen-2-yl)-isonicotinoylhydrazide) (C8). For these compounds, interference in the viability of eggs was observed, as well as which were the smallest amounts of the product to cause 50% and 90% of mortality in the population with estimated LC50 values of 5.77, 6.15, 6.62 and 8.16 µg/mL, and LC 90 with values of 23.77; 18.41; 20.38 and 31.55, respectively. Therefore, the following order of acaricidal activity was observed in eggs of the green mite, M. Tanajoa C7 > C6 > C4 > C8 > C9 > C5 > C1 > C2 > C3. Our studies showed that C7, C6 and C4 showed efficient results regarding the toxicity of eggs and adults of M. tanajoa. The structure-activity relationships between the most promising compounds show that the presence of sulfonamide-type substituents linked to position 2 of the quinone nucleus (C7 and C6) allowed an increase in activity by 5.5 and 3.2 times, respectively, compared to 1,4-naphthoquinone itself (C1). On the other hand, the inclusion of the bromine atom at position 2 of 1,4-naphthoquinone was able to increase the activity by 2.7 times compared to C1. In addition, C8 (N'-(1,4-Dihydro-1,4-dioxonaphthalen-2-yl)-isonicotinoylhydrazide) and C9 (N-Isonicotinoylhydrazone-[2-hydroxy-3-(3-methyl-2-butenyl) )]-1,4-naphthoquinone) showed that the change of the hydroxyl group or one of the carbonyl groups of the basic nucleus of naphthoquinones (C2 and C3 compounds) by a polytrogenated group, be it a 2-N-acylhydrazide (C8) or a 1-N-acylhydrazone (C9) contributed to increase the toxicity of the compounds in the eggs in relation to the adult phase of M. Tanajoa, with the presence of the 2-N-acylhydrazone group being more efficient than 1-N-acylhydrazone. The ADME in silico properties of naphthoquinone compounds showed potential to develop as good candidates for acaricides, following Lipinski's rule of five. Repellent effect was observed for natural naphthoquinones, lausone (C2) and lapachol (C3) only from CLs 90 and 95 respectively. However, for C8 and C9 naphthoquinones synthesized from natural compounds, repellency was observed from LC25. In relation to the other synthetic naphthoquinones 1,4 Naphthoquinone (C1), 2-bromo-1,4-naphthoquinone (C4), 2, 3-Dichloro-1, 4-naphthoquinone (C5), 4-Pyridin-3-Il) (Methylsulfonyl) Phenylamino) Naphthalene 1,4-Dione (C6) and 2-(4-((5-Methyloxazol-3-yl) Methylsulfonyl) Phenylamino) Naphthalene-1,4-Dione) (C7) repellent effect was observed M. tanajoa when using contractions referring to CLs 75, 90 and 95. The instantaneous population rate for all compounds decreased with increasing concentrations. Compounds C5 in LC 90 and 95 and compound C7 in LC 95 were able to stabilize the population of M. tanajoa (ri=0). None of the natural compounds C2 (lausone) and C3 (lapachol) tested caused the instantaneous rate of population growth to be negative, however, there was a proportional reduction in the reproduction of mites, with increasing concentrations used. However, compounds C8 and C9 that were synthesized from lausanne and lapachol, respectively, were able to negatively destabilize the population of M. tanajoa. In the residual effect, it was observed that only C3 ([2-Hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthatoquinone - Lapachol) showed moderate residual persistence (mortality ≥ 33% up to 16 days after the application of the products) and the naphthoquinone compounds C1, C2, C4, C5, C6, C7, C8 and C9 show slight persistence (mortality ≥ 27% up to 8 days after the application of the compounds). Naphthoquinones may have potential for the development of acaricides. However, further studies should be carried out to assess the expense and efficiency of these naphthoquinones on a wide variety of pests in the field.
|
|
2
|
-
MARIA JUSSARA DOS SANTOS DA SILVA
-
Characterization and alternative control of Fusarium species
associated with Damping-off in coriander
-
Leader : GAUS SILVESTRE DE ANDRADE LIMA
-
MEMBRES DE LA BANQUE :
-
EDNA PEIXOTO DA ROCHA AMORIM
-
GAUS SILVESTRE DE ANDRADE LIMA
-
JAQUELINE FIGUEREDO DE OLIVEIRA COSTA
-
SARAH JACQUELINE CAVALCANTI DA SILVA
-
WELLINGTON COSTA DA SILVA
-
Data: 17 févr. 2023
-
-
Afficher le Résumé
-
Coriander is a vegetable that has its cultivation consolidated among rural producers in northeastern Brazil. However, the incidence of disease caused mainly by pathogens transmitted by the soil, becomes one of the limiting factors for production. Some problems caused by these phytopathogens is the reduction of the stand, due to the damping off of seedlings. There are still no concrete studies on the specific cause of the disease in culture. However, knowing the etiological agent is essential for the development of control methods. Therefore, the objective of this work was to identify and characterize phytopathogenic agents associated with damping-off of coriander seedlings in the states of Alagoas, Pernambuco and Ceará, and to evaluate their in vitro sensitivity to essential oils of clove (Eugenia caryophyllus), cinnamon (Cinnamomum cassia), citronella (Cymbopogon winterianus) and melaleuca (Melaleuca alternifolia). Soils were collected in different coriander producing areas, taken to the laboratory where they were distributed in 300 vases with a capacity of 250mL and identified according to the municipality of collection. To obtain the isolates, six coriander seeds of the Verdão cultivar (Isla seeds LTDA) were sown in each pot. Seed germination occurred seven days after planting and, 10 days after germination, the seedlings showed symptoms of wilting followed by damping off, thus performing the indirect isolation of pathogens. In the test to assess sensitivity F. fabacearum and F. falciforme, the experimental design was completely randomized, in a 4x6 factorial scheme (4 essential oils x 6 concentrations), with five replications, with the experimental unit consisting of a Petri dish. Treatments were generated by combining concentrations (0; 12.2; 25; 50; 100 and 200 μL mL-1) of essential oils. The plates were inoculated with the pathogens for seven days at 25 ± 2 °C. To verify the difference between treatments, the mycelial growth velocity index (IVCM) was calculated. Using morphocultural and molecular analyzes with specific primers for the RPB2 and TEF genes, a
total of thirty-eight Fusarium isolates were obtained. Of these, thirty isolates clustered in the F. oxysporum complex (one isolate of F. elaeidis; one isolate of F. gossypinum and twenty-eight isolates of F. fabacearum) and eight isolates in the F. solani complex, together with the species F. sickle cell. In the sensitivity test, cinnamon essential oil was the one that showed the best result from the concentration of 50 μL mL- with fungitoxic action on mycelial growth for the species F. fabacearum and F. falciforme when compared to the other oils. Based on this, this is the first report of F. fabacearum, F. gossypinum and F. elaidis causing damping off in coriander seedlings in the world and cinnamon essential oil shows promise for the alternative control of damping off in coriander crops.
|
|
3
|
-
PAULO HENRIQUE TAVARES SANTOS FARIAS
-
Resistance evaluation in accessions of the complex Saccharum spp. to the small borer Diatraea saccharalis (Fab. 1794) (Lepidoptera: Crambidae)
-
Leader : ELIO CESAR GUZZO
-
MEMBRES DE LA BANQUE :
-
ELIO CESAR GUZZO
-
MARIANA OLIVEIRA BREDA
-
ALICE MARIA NASCIMENTO DE ARAUJO
-
ALDOMARIO SANTO NEGRISOLI JUNIOR
-
JOÃO GOMES DA COSTA
-
Data: 24 févr. 2023
-
-
Afficher le Résumé
-
The search for renewable energies has transformed the current agricultural scenario and, as a result, much emphasis is given to the sugarcane crop, which, in practically all its phenology, is infested by borers, which are key pests for the culture. Thus, researches that corroborate with varieties resistant to these insects become of fundamental importance for their management in production systems. The present study aims to characterize genotypes of the Saccharum spp. and biotic stresses caused by borers. Therefore, we evaluated the intensity of infestation of the giant borer Telchin licus (Drury, 1773) (Lepidoptera: Castniidae) in a commercial area in São Miguel dos Campos, AL during the 2021 and 2022 harvest, analyzing the total amount of internodes and the number of brocade internodes. The larval antiobiosis of D. saccharalis (Fab. 1794) (Lepidoptera: Crambidae) was evaluated from an experimental planting of the genotypes in a greenhouse at the Research Execution Unit of Embrapa Tabuleiros Costeiros (UEP de Rio Largo, AL). In the laboratory, two experiments were carried out: the biology and construction of the fertility life table of D. saccharalis, fed with an artificial diet, produced from the studied sugarcane genotypes and the biology of its parasitoid Cotesia flavipes Cameron (Hymenoptera: Braconnidae), from the parasitism of caterpillars fed the aforementioned diets.
|
|
4
|
-
TACIANA FERREIRA DOS SANTOS
-
M
MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF FUNGAL SPECIES ASSOCIATED WITH PLANTS OF THE ORCHIDACEAE FAMILY
-
Leader : IRAILDES PEREIRA ASSUNCAO
-
MEMBRES DE LA BANQUE :
-
ANA FRANCISCA TIBÚRCIA AMORIM FERREIRA E FERREIRA
-
IRAILDES PEREIRA ASSUNCAO
-
JAQUELINE FIGUEREDO DE OLIVEIRA COSTA
-
MARIA DE FATIMA SILVA MUNIZ
-
SARAH JACQUELINE CAVALCANTI DA SILVA
-
Data: 24 févr. 2023
-
-
Afficher le Résumé
-
Floriculture is one of the fastest growing agribusiness segments in Brazil, especially the tropical flower market, which is favored by the climatic conditions of the different regions of the country, with emphasis on the northeast region. Within this sector, the production of orchids represents a type of trade in increasing expansion in the national market and this is due to the diversity, beauty of its flowers and its easy cultivation. However, despite the progress, orchid culture has been limited by several factors, among which is the occurrence of diseases, especially those of fungal origin. Knowing the etiological agent is essential for the success, durability and implementation of control measures. Therefore, the objective of the present work was to identify species of fungi associated with orchids through morphocultural and phylogenetic characteristics. The isolates were obtained from native orchids or from commercial plantations showing typical symptoms of diseases with fungal etiology. Subsequently, morphological and cultural studies and pathogenicity tests were carried out in asymptomatic plants belonging to different genera of the Orchidaceae family, followed by DNA extraction and amplification of partial sequences of the glyceraldehyde3-phosphate dehydrogenase (GAPDH) genes. , translation elongation factor 1-α (TEF-1α) and second major RNA polymerase II subunit (RPB2). A total of 80 isolates were obtained, 57 belonging to the genus Colletotrichum, 12 to Fusarium and 11 to pestalotioid fungi. Of these, 11 isolates of Colletotrichum, 5 of Fusarium and 5 of pestalotioid fungi were destined for phylogenetic studies. Colletotrichum cultures showed mycelium varying between shades of gray, yellow and white, with hyaline, non-septate, straight-cylindrical conidia and rounded ends. The Fusarium isolates produced mycelium in shades of white, pink or purple, with hyaline macro and microconidia. While the pestalotioid fungi formed purely white colonies or with an orange reverse, cottony and conidia presenting 2 to 3 apical appendages and a basal appendage. The observation of the morphological and cultural characteristics, together with the phylogenetic analysis of the GAPDH gene, could group the Colletotrichum isolates into the complexes C. gloeosporioides, C. orquidearum, C. gigasporum and C. dracaenophilum; The Fusarium isolates were grouped with species of the F. incarnatum–equiseti complex, F. fujikuroi and F. oxysporum, based on the phylogeny of the partial sequences of the TEF-1α and RPB2 genes. While the pestalotioid fungi clustered with Neopestalotiopsis and Pseudopestalotiopsis species through TEF-1α phylogenetic analysis.
|
|
5
|
-
TIAGO SILVA LIMA
-
IDENTIFICATION OF Colletotrichum spp. ASSOCIATED WITH ANTHRACNOSIS IN HELICONIA AND ASSESSMENT OF SENSITIVITY TO FUNGICIDES.
-
Leader : IRAILDES PEREIRA ASSUNCAO
-
MEMBRES DE LA BANQUE :
-
IRAILDES PEREIRA ASSUNCAO
-
SARAH JACQUELINE CAVALCANTI DA SILVA
-
EDNA PEIXOTO DA ROCHA AMORIM
-
MARIA DE FATIMA SILVA MUNIZ
-
JULIANA PAIVA CARNAUBA
-
Data: 24 févr. 2023
-
-
Afficher le Résumé
-
Diseases are an important obstacle faced by heliconia producers, causing significant losses. The anthracnose disease, for example, is caused by fungi of the genus Colletotrichum, responsible for causing damage due to the difficult control, since the symptoms make the flower stems unsuitable for commercialization. Considered as an important plant pathogen, the genus Colletotrichum has a wide diversity of species, being responsible for causing a variety of diseases in several economic crops and ornamental plants, making it difficult to control this disease. The understanding and knowledge of which species of Colletotrichum are occurring in heliconia, as well as the infectious behavior of these species in different hosts and the effect of different fungicides on them is important for the development of more efficient control strategies. However, identification studies of this genus in heliconias are still scarce. Therefore, the objective of this work was to identify species of the genus Colletotrichum associated with heliconias through the use of molecular tools and morphocultural characteristics, as well as to evaluate the effect of different fungicides on mycelial growth and conidia germination. Fungal isolates were obtained from heliconia plants with anthracnose symptoms, in the states of Alagoas, Ceará and Pernambuco. These isolates will be characterized through multi-locus phylogenetic analysis and mold-cultural characters of the species. In vitro tests with the fungicides (Azoxistrobina + difenoconazol), (Clorotalonil), (Fluxapiroxade + Piraclostrobina) and (Difenoconazole) will be carried out to evaluate the inhibition of mycelial growth by the method of incorporation of fungicides in the PDA culture medium and the inhibition of conidia germination using excavated slides containing water with fungicide. Thirty-two isolates were obtained so far from flowers, leaves, petioles and bracts showing typical symptoms of anthracnose. The initial analysis of the partial sequences of the GAPDH gene was performed with seventeen isolates of Colletotrichum, the sequences were compared with the accesses of the GenBank database using the BLASTn algorithm, together with the construction of Bayesian Inference trees, it was possible to group the isolates in four complexes, two isolated in the C. boninense complex, twelve in the C. gloeosporioides complex, two in the C. magnum complex and one in the C. gigasporum complex. All isolates were able to develop symptoms on inoculation with wounds, inducing depressed, waterlogged, light brown, and variable sized necrotic lesions on leaves. With the study it is expected to know the diversity of species of Colletotrichum associated with the heliconia culture, obtaining relevant information about the pathosystem for the development of adequate strategies for the control of the disease.
|
|
6
|
-
JOSÉ TOMÁZ FERREIRA NUNES
-
ENDOPHYTIC ISOLATES IN GENOTYPES OF Phaseolus lunatus L.
-
Leader : GILDEMBERG AMORIM LEAL JUNIOR
-
MEMBRES DE LA BANQUE :
-
EDNA PEIXOTO DA ROCHA AMORIM
-
GILDEMBERG AMORIM LEAL JUNIOR
-
JOÃO MANOEL DA SILVA
-
MAYRA MACHADO DE MEDEIROS FERRO
-
SARAH JACQUELINE CAVALCANTI DA SILVA
-
Data: 27 févr. 2023
-
-
Afficher le Résumé
-
Even with some inconsistency among the authors about the concept that best characterizes an endophytic, the most usual is that endophytic fungi are those that live, in at least part of their life cycle, inside plant tissues, without apparently causing any harm to their hosts. These definitions began to be discussed with the mycologist De Bary in 1866, but further studies of these organisms did not begin until about a hundred years later, around 1970. Currently, many aspects of endophytic fungi are well established, but there is still a vast field to be explored. Understanding this complex relationship between endophytic and host is fundamental so that, possibly, we can use these organisms in our favor in different fields of science, such as, for example, in the biological control of anthracnose in fava beans. The objectives proposed here aimed to contribute to the advancement of knowledge about the interaction between the lima bean crop and its endophytic fungi, providing opportunities for future biotechnological applications of endophytic fungi in a perspective of developing products and biotechnological processes of interest to agriculture. In the present study, cultivable fungi isolates, which are likely to be beneficial for plant growth, health, and productivity, will be identified, and whether these endophytes vary between plant organs and broad bean genotypes from an Andean and Mesoamerican material, also contrasting for resistance to anthracnose. The study will gather basic information on the occurrence and diversity of endophytic fungal species in new and old leaves, flowers, and fruits of fava genotypes. The isolation and identification of cultivable endophytes may contribute to future studies of the function and application of endophytic fungi in resistance to biotic and abiotic stresses. (AZAD and KAMINSKY, 2016; ABDEL-MONAIN et al., 2014; BERG et al., 2014). Aiding in the development of new, efficient, and sustainable strategies to improve the quality and productivity of crops, while reducing the application of harmful chemicals. In article I, “Diversity of endophytic fungi in Phaseolus lunatus”, the composition and evolution of endophytic fungi during the vegetative and reproductive phases of the host in distinct and contrasting gene pools regarding resistance to anthracnose is presented. Endophytes were isolated from asymptomatic without disease symptoms, namely: new leaves, old leaves, flowers, and pods. With the morphocultural and molecular characterization, the isolates were described until their respective genera. And in Article II, “Prevalence of Colletotrichum ssp. endophytic in broad bean”, a phylogenetic evaluation of endophytic fungi of the genus Colletotrichum is described, and the use of different genomic regions is proposed to evaluate the genetic variability of fungi of this genus.
|
|
7
|
-
JOAIS JOSÉ DA SILVA
-
RESISTANCE INDUCTION BY INOCULATION OF THE FUNGUS Cunninghamella elegans AND INFLUENCE OF NITROGEN FERTILIZATION ON RESISTANCE OF Phaseoulus lunatus To Colletotrichum truncatum
-
Leader : GILDEMBERG AMORIM LEAL JUNIOR
-
MEMBRES DE LA BANQUE :
-
JAMILLY ALVES DE BARROS
-
GILDEMBERG AMORIM LEAL JUNIOR
-
JAQUELINE FIGUEREDO DE OLIVEIRA COSTA
-
JULIO ALVES CARDOSO FILHO
-
SARAH JACQUELINE CAVALCANTI DA SILVA
-
Data: 28 févr. 2023
-
-
Afficher le Résumé
-
The fava beans, or fava beans, originates in Guatemala in Central America, being cultivated mostly by small producers. It is the second legume of greatest socioeconomic importance due to its food properties considered beneficial to human health, being consumed in the form of ripe or green grains. However, diseases such as anthracnose (Colletotrichum spp.) can cause a considerable reduction in production, which causes both direct and indirect damages during the biological cycle of the crop. Most of the time, phytosanitary chemicals are used for its control. And the use of phytosanitary chemicals, provides adverse effects such as the development of resistant populations, outbreaks of secondary diseases, water and soil contamination, negative impact on biodiversity. Another problem is nutritional imbalance. Mineral nutrients can affect plant growth and production, as they are responsible for specific functions in metabolism. And an adequate and balanced nutritional fertilization of nutrients can increase the resistance of plants to diseases. The nutrient most required by plants is nitrogen, which must be associated with proteins and lipids. The objectives of this work are to evaluate the effect of resistance induction by inoculation of the fungus C. elegans, in comparison with applications of commercial chitosan and Ecolife® on the severity of anthracnose in fava beans. And to evaluate the influence of nitrogen fertilization on the resistance of P. lunatus to C. truncatum. Experiments were carried out with the application of the inducers via soil and via leaf. And two experiments, one to evaluate the effect of the dose and the other to evaluate the effect of the source of nitrogen fertilization on the severity of anthracnose in fava beans. Data will be submitted to analysis of variance (ANOVA), and when significant will be submitted to comparison of means by the Scott-Knott test at 5% error probability, with the statistical analysis program SISVAR.
|
|
8
|
-
ELMADÃ PEREIRA GONZAGA
-
STUDY OF THE SEXUAL PHEROMONE OF Thyrinteina arnobia (STOLL, 1782) (LEPIDOPTERA: GEOMETRIDAE) IN EUCALYPTUS
-
Leader : MARIANA OLIVEIRA BREDA
-
MEMBRES DE LA BANQUE :
-
ALICE MARIA NASCIMENTO DE ARAUJO
-
HENRIQUE FONSECA GOULART
-
ISIS TORRES SOUZA
-
JAKELINE MARIA DOS SANTOS
-
MARIANA OLIVEIRA BREDA
-
ROSEANE CRISTINA PREDES TRINDADE
-
Data: 28 févr. 2023
-
-
Afficher le Résumé
-
The genus Eucalyptus has about 600 species, adapted to the most varied climates and soils. In Brazil, around 7.47 million hectares are occupied by eucalyptus forest stands. With the advance of eucalyptus cultivation in Brazil, the incidence of population outbreaks of insects has been favored. Among the insect pests of the crop, the defoliator caterpillar Thyrinteina arnobia (Stoll, 1782) (Lepidoptera: Geometridae) stands out, cited as the most important lepidopteran for eucalyptus cultivation in the country. In the state of Alagoas, the presence of T. arnobia was found to cause significant damage. Due to the large extension of the eucalyptus forest stands and the height of the trees, the chemical control of T. arnobia becomes economically unsustainable. As a result, new control methods have been proposed, including behavioral control, through the study of the sexual pheromone of T. arnobia. Therefore, it is necessary to understand the mechanisms involved in the chemical ecology of the species, from the sexual pheromone release process, through the perception of these compounds, excitation and response to pheromonal substances. Thus, the present study aims to: (i) provide basic and complementary information on the sexual behavior of T. arnobia, based on the observation of the calling behavior; (ii) carry out the study of antennal sensilla in males and females of T. arnobia, through scanning electron microscopy (SEM); (iii) isolate and identify possible pheromonal substances from extracts of abdominal glands of T. arnobia females, using gas chromatography with flame ionization detector (GC-FID); (iv) and verify the bioactivity of the extracts, through electroantenography coupled to gas chromatography (EAG-GC). Regarding calling behavior, gland exposure occurred after the 2nd hour of scotophase. The calling behavior was characterized by the exposure of the pheromone gland of the females of T. arnobia, the dissemination of the sexual pheromone, the perception of the pheromone by the males and the approach of the males to the emitting source of the pheromone. In total, six types of sensilla were identified in antennae of females and males of T. arnobia, characterized in trichoid, squamiform, caetic, basiconic, celoconic and styloconic. The antennae of female and male T. arnobia have different structural morphology of sensilla. Antennae of both sexes have Böhm's bristles at the base of the scape. The trichoid sensilla are the most abundant, mainly in the antennae of T. arnobia males, indicating that they can respond to stimuli from chemical substances. In the chromatographic profile of the extract of abdominal glands in T. arnobia females from Alagoas, it was possible to previously identify 19 possible compounds. Regarding the bioactivity of pheromonal substances from extracts of abdominal glands of T. arnobia females, it was observed that the electroantennary responses of male antennae submitted to the pheromonal extract of T. arnobia were significant when compared to the hexane stimulus (control).
|
|
9
|
-
MARIA HELLOÁ COSTA DE OLIVEIRA
-
NEXT GENERATION SEQUENCING IN DETERMINING THE DIVERSITY OF BADNAVIRUS THAT INFECT TROPICAL FLOWERS IN THE NORTHEAST REGION
-
Leader : SARAH JACQUELINE CAVALCANTI DA SILVA
-
MEMBRES DE LA BANQUE :
-
GAUS SILVESTRE DE ANDRADE LIMA
-
GILDEMBERG AMORIM LEAL JUNIOR
-
JOÃO MANOEL DA SILVA
-
MAYRA MACHADO DE MEDEIROS FERRO
-
SARAH JACQUELINE CAVALCANTI DA SILVA
-
Data: 31 juil. 2023
-
-
Afficher le Résumé
-
One of the most promising segments of the national agribusiness is floriculture, with an emerging market of profitability all over the world. Tropical flowers are attacked by different pathogens, including badnaviruses. Badnaviruses (Family Caulimoviridae) consist of a group of viruses with a double circular DNA genome, encapsulated in non-enveloped bacilliform particles, and represented mainly by mealybug strands. Diseases dangerous to the world by serious viruses are in serious genera in tropical crops important in tropical ones, including Brazil. To characterize badnavirus species that occur in tropical plants of the Cannaceae, Heliconiaceae, and Zingiberaceae families, they were collected in the poles that produce tropical plants in Alagoas, Pernambuco and Ceará. Subjected to badnavirus detection via PCR) using the universal pink primers for the genus, twelve samples (“Cost. by virus in the invalid virus infection. Once identified as infected with the virus in the episomal form, the RCA products were selected and will be sequenced by NGS. How SDT sequences and genome comparisons sequences will be approved and viral for sequence analysis and SDT genome comparisons for species demarcation. To confirm the presence of infections identified by the NGS and ensure the safety of the method, design PCR primers for each virus designed for full signatures for the full genome via primer walking sequencing.
|
|
10
|
-
CAMILA ALEXANDRE CAVALCANTE DE ALMEIDA
-
BASES FOR THE IMPLEMENTATION OF BIOLOGICAL CONTROL OF Thyrinteina arnobia (Stoll, 1782) (LEPIDOPTERA: GEOMETRIDAE)
-
Leader : MARIANA OLIVEIRA BREDA
-
MEMBRES DE LA BANQUE :
-
ALICE MARIA NASCIMENTO DE ARAUJO
-
ELIO CESAR GUZZO
-
HENRIQUE FONSECA GOULART
-
JAKELINE MARIA DOS SANTOS
-
MARIANA OLIVEIRA BREDA
-
VANDERSON BARBOSA BERNARDO
-
Data: 20 déc. 2023
-
-
Afficher le Résumé
-
Among the pest insects associated with eucalyptus culture, the brown eucalyptus caterpillar, Thyrinteina arnobia (Stoll, 1782) (Lepidoptera: Geometridae), derived from native myrtaceous trees, such as guava (Psidium guajava L.), is considered the main defoliating species of the genus Eucalyptus in the country. In this sense, different methods that fit the premises of the Integrated Management of Forest Pests (IPM- Forest) have been the focus of research, including the use of biological control, through the conservation or multiplication and release of natural enemies. The gregarious endoparasitoid Palmistichus elaeisis La Salle (Hymenoptera: Eulophidae) is considered a promising agent in the control of defoliating lepidoptera, having generalist habits. For complete elucidation of the interactions that permeate the use of parasitoids in biological control programs, the understanding of the host search process is fundamental. In view of the above, the objective of this study was to carry out survey studies of natural enemies of spontaneous occurrence of T. arnobia in forest stands of Eucalyptus spp., in addition to the evaluation of the chemical ecology in the use of the endoparasitoid P. elaeisis, aiming at directing the bases for the planning and implementation of the conservative and augmentative biological control of T. arnobia in the state of Alagoas. It was possible to register the spontaneous occurrence of the natural enemies, Winthemia sp. (Diptera: Tachinidae), Charops sp. (Hymenoptera: Ichneumonidae, Campopleiginae), Glyptapanteles sp. (Hymenoptera: Braconidae) and the predator Podisus sp. (Hemiptera: Pentatomidae) associated with T. arnobia in Eucalyptus sp. in the state of Alagoas. Compounds found in pupae of T. arnobia, indicated as 4-Hexen-1-ol, acetate; 2,3,5,6-Tetramethylpyrazine; 2-Nonanone and 1-Undecene present bioactivity in processes of attraction of the parasitoid P. elaisis. The results obtained contribute to the consolidation of the Integrated Management of Forest Pests (IPM- Forest).
|
|