PPGPP PROGRAMA DE PÓS-GRADUAÇÃO EM PROTEÇÃO DE PLANTAS CAMPUS DE ENGENHARIA E CIÊNCIAS AGRÁRIAS Téléphone/Extension: 99963-8987
Dissertation/Thèse

Clique aqui para acessar os arquivos diretamente da Biblioteca Digital de Teses e Dissertações da UFAL

2024
Thèses
1
  • ESTER LETICIA DA SILVA AMARAL
  • RESISTANCE OF CREOLE MAIZE VARIETIES TO Sitophilus zeamais Mots., 1855 (COLEOPTERA: CURCULIONIDAE).

  • Leader : MARIANA OLIVEIRA BREDA
  • MEMBRES DE LA BANQUE :
  • JOÃO GOMES DA COSTA
  • MARIANA OLIVEIRA BREDA
  • ROSEANE CRISTINA PREDES TRINDADE
  • Data: 16 févr. 2024


  • Afficher le Résumé
  • The mayze crop (Zea mays L.) is of great importance in rural settlements and in different forms of social organization of production, including family, indigenous and quilombola agriculture, considered maintainers and guardians of the variability and natural biodiversity of cultivated crops with the maintenance and multiplication of creole seeds, characterized by local varieties, cultivated through generations. Among the phytosanitary problems faced in the storage of mayze seeds, the corn weevil, Sitophilus zeamais Mots., 1855 (Coleoptera: Curculionidae), is considered a key pest of corn in all producing regions, causing several quantitative and qualitative damage. Thus, the evaluation of the resistance of creole mayze varieties to S. zeamais attack was carried out through host preference bioassays, susceptibility index (SI), instantaneous population growth rate (ri), weight loss (g), in addition to determining moisture (%) and ash (%) contents. The results obtained demonstrated greater host preference for the creole variety Palha Roxa and lower preference of S. zeamais for the commercial hybrid Caatingueiro. The state of use of the corn grains in the bioassays, (i) unattacked and intact grains, (ii) unattacked and broken grains, (iii) attacked and intact or (iv) attacked and broken grains also influenced the choice of S. zeamais. In general, susceptibility indices ranged from 0.01 for the Caatingueiro variety to 0.034 for the Jabotão creole variety, corroborating the instantaneous population growth rates, varying between 0.026 for the commercial hybrid Caatingueiro and 0.046 for the Palha Roxa creole variety. The Caatingueiro variety also showed lower weight loss (35.98g), while Palha Roxa (188.76g) showed significantly greater weight loss, in addition to high moisture (%) and ash (%) content.

2
  • JOSÉ CARLOS SANTOS SILVA
  • Monitoring of insect pests and technical feasibility studies for the production of soybean seeds in the state of Alagoas

  • Leader : ELIO CESAR GUZZO
  • MEMBRES DE LA BANQUE :
  • ELIO CESAR GUZZO
  • PAULO DE ALBUQUERQUE SILVA
  • ROSEANE CRISTINA PREDES TRINDADE
  • Data: 28 mars 2024


  • Afficher le Résumé
  • Soybean is the main product of Brazilian agribusiness, mainly used to obtain grains, oil and bran. In recent years, the SEALBA region has stood out in the production of this grain. In Alagoas, the crop has been occupying the idle agricultural areas left by the recent crisis in the sugar and alcohol sector, with the prospect of an increase in production to supply seeds to the main traditional production areas of the country, due to its different climate regime. However, the technical viability of soybean seed production in the state has never been assessed, and phytosanitary problems represent an important demand recently highlighted by the state's production chains. Thus, the present project proposes to investigate the main species of insect pests and diseases associated with the production of soybean seeds, as well as the sanitary parameters and the physiological and biochemical quality of soybean seeds produced in the edaphoclimatic conditions of the state of Alagoas.

Thèses
1
  • YOLANDA DE MELO DE OLIVEIRA
  • Transmission, incidence and molecular characterization of Begomovirus associated with Phaseolus lunatus in the Northeast region.

  • Leader : IRAILDES PEREIRA ASSUNCAO
  • MEMBRES DE LA BANQUE :
  • FREDERICO MONTEIRO FEIJÓ
  • GILDEMBERG AMORIM LEAL JUNIOR
  • IRAILDES PEREIRA ASSUNCAO
  • MILENY DOS SANTOS DE SOUZA
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • Data: 26 févr. 2024


  • Afficher le Résumé
  • The genus Phaseolus belongs to the Fabaceae family, originating in the Americas, having around 55 species. Among these species, common bean (Phaseolus vulgaris) and lima bean (Phaseolus lunatus) are the most economically important and cultivated in Brazil. Beans play an important role in human nutrition, being a cheaper source of protein compared to meat, eggs and fish. The genus Begomovirus (family Geminiviridae) is among the most harmful pathogens that infect these crops, being considered a limiting factor in producing areas. In Brazil, the most economically important viruses that infect these hosts are Bean golden mosaic virus (BGMV) and Macroptilium yellow spot virus (MaYSV). Begomoviruses are transmitted by a complex of cryptic species of Bemisia tabaci, causing direct and indirect damage, limiting the production of this legume. In this context, the present study aims to identify, at the species level, the Begomoviruses that naturally infect P. vulgaris and P. lunatus collected in the Northeast region and to determine host attractiveness and virus transmission using Bemisia tabaci, Middle East-Asia Minor (MEAM1). Leaf samples of P. vulgaris and P. lunatus were collected in the states of Alagoas, Sergipe and Pernambuco, in Northeastern Brazil. Total DNA was extracted and used as a template for PCR using specific primers for BGMV and MaYSV, followed by amplification of viral genomes by rolling circle. These genomes will be commercially cloned and sequenced by primer walking. To correctly attribute taxonomy to the new isolates, paired comparisons of the complete genome with other previously reported begomoviruses and phylogenetic analysis will be used. A colony of B. tabaci will be established, which will be used in transmission and attractiveness tests with noncultivated plants. For attractiveness and transmission tests, the following species will be used: Senna occidentalis, Blainvillea latifolia, Macroptilium lathyroides, Hyptis suaveolens, Chenopodium album, Desmodium tortuosum, Phaseolus lunatus and Solanum lycopersicum. Heavily infested cabbages will be placed between the plants for a week, the infestation will be accounted for by counting the number of eggs, nymphs and adults present on the leaflets, other parameters will also be recorded, including the growth of sooty mold, the damage caused to the leaves and the nymph colonization. For the transmission test, whiteflies will be fed on a Macroptolium sp. plant, infected with begomovirus, for a period of 24 hours (acquisition access period) and then placed to feed on each species of plant used in the study, individually for 48 hours (period of access to inoculation). Initially, the presence of symptoms will be evaluated and, 30 days after infection, leaf samples will be collected for total DNA extraction and confirmation by PCR with species-specific primers.

2
  • EUZANYR GOMES DA SILVA
  • DIVERSITY AND MYCOTOXIGENIC POTENTIAL OF SPECIES OF
    FUSARIUM FUJIKUROI COMPLEX ASSOCIATED WITH BANANA CROWN ROT

  • Leader : KAMILA CÂMARA CORREIA
  • MEMBRES DE LA BANQUE :
  • CAMILA PRIMIERI NICOLLI
  • AILTON REIS
  • IRAILDES PEREIRA ASSUNCAO
  • KAMILA CÂMARA CORREIA
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • Data: 27 févr. 2024


  • Afficher le Résumé
  • Banana (Musa spp.), is one of the most explored fruits in Brazil and in the world and is
    consumed worldwide due to its nutritional importance. However, its commercialization
    is seriously affected by post-harvest rots that cause considerable damage and losses. The
    disease is associated with fungal complexes, especially as Fusarium is one of the most
    important fungal genera of plant pathogens that affect the cultivation of a wide range of
    crops and produce various mycotoxins. Several species have already been reported as
    pathogenic of crown rot, among these species are within the Fusarium fujikuroi species
    complex (FFSC) which are major producers of mycotoxins, mainly fumonisins (FBs) and
    of great clinical importance. Thus, the objective of the study is to characterize the FFSC
    species with a molecular approach of isolates of banana crown rot, collected in shops and
    processing units in the Cariri region of Ceará, to identify their pathogenicity and to
    evaluate their production potential. of mycotoxins through identification of FUM genes
    using specific primers in PCR analysis. To identify the Fusarium species, obtained from
    crown rot were subjected to DNA extraction and sequenced for two gene regions (TEF
    and RPB2). After identification, the isolates were tested for the presence of genes
    involved in the production of fumonisins using specific primes (FUM53F/FUM53F and
    VERT1/VERT2) and then the pathogenicity and aggressiveness of the isolates were
    evaluated. Phylogenetic analyzes characterized 64 isolates belonging to the FFSC,
    showing that the most frequent species were F. annulatum, F. musae and F. sacchari,
    after F. verticillioides and F. pseudocircinatum. This is the first report of the F. annulatum
    species associated with crown rot in the world. All isolates were analyzed for the presence
    of the FUM1 and FUM8 genes. Nine isolates were positive for the genes, the species F.
    verticillioides, F. musae and F. sacchari showed the presence of the FUM genes, having
    the ability to produce fumosinin. All species were pathogenic, except for F. verticillioides.
    There were significant differences in relation to the aggressiveness of the isolates, six
    isolates were more aggressive identified as F. musae, F. annulatum and F. sacchari. This
    study brings new contributions on the diversity of etiological agents associated with
    crown rot in the Cariri region of cearense, FFSC members, and the ability of these species
    to produce fumonisins. Considering the relevance that banana is one of the fruits most
    present in the diet of the Brazilian population, our results contribute with new knowledge
    for the future evaluation of quantification of mycotoxins in banana fruits in Brazil. Since,
    this study indicates a potential threat to the quality of Brazilian banana.

3
  • JACKELINE LAURENTINO DA SILVA
  • SUSCEPTIBILITY OF Colletotrichum SPECIES ASSOCIATED WITH PASSION FRUIT ANTHRACNOSE USING CHEMICAL AND BIOLOGICAL CONTROL.

  • Leader : IRAILDES PEREIRA ASSUNCAO
  • MEMBRES DE LA BANQUE :
  • EDNA PEIXOTO DA ROCHA AMORIM
  • IRAILDES PEREIRA ASSUNCAO
  • JULIANA PAIVA CARNAUBA
  • MARIA DE FATIMA SILVA MUNIZ
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • Data: 28 févr. 2024


  • Afficher le Résumé
  • Passion fruit (Passiflora spp.) is one of the main tropical fruits grown in Brazil, in addition to being the world's leading producer of passion fruit. Anthracnose, caused by species of the genus Colletotrichum, is one of the main factors contributing to the reduction of production. Knowing the importance of this pathogen for culture and the great diversity of species of this fungal genus, it is essential to develop appropriate techniques to control the disease. Therefore, the objectives of this study were to evaluate in vitro and in passion fruit seedlings the effects of the fungicides Difenoconazole, Pyraclostrobin, Flutriafol and Azoxystrobin + Difenoconazole, Trichoderma harzianum and Bacillus spp. on Colletotrichum spp. In the in vitro evaluation of the sensitivity of Colletotrichum spp. to fungicides, the pathogens were inoculated in BDA culture medium supplemented with the concentrations established for each fungicide. In the in vivo evaluations, healthy seedlings of passion fruit from the Cerrado were used in preventive and curative treatments. Evaluating Trichoderma in the pairing test, filter paper discs moistened in the suspension of Colletotrichum spores (2x106 conidia/mL) were used and placed on the edge of Petri dishes containing synthetic Potato-Dextrose-Agar (BDA – Kasvi) culture medium. After 72 hours, the same procedure was performed using Trichodermil SC 1306 ® (T. harzianum), at concentrations of 0.5; 1.0 and 1.5 L/ha, placing the moistened paper disc in the opposite direction to the phytopathogens.  The control was composed only of the plate with the phytopathogens. To evaluate the action of metabolites, plugs containing structures of each Colletotrichum species were placed in the center of Petri dishes, separately. After 48 hours, Trichoderma harzianum plugs were placed on plates containing synthetic PDA medium. The bases of the plates containing antagonist and phytopathogens were superimposed, joined and sealed. As a control, a base containing a mycelium disk containing the structures of the phytopathogens and the other containing only one disk of BDA culture medium was superimposed. For the antagonism test using Bacillus spp, pathogen plugs and bacterial growth were inoculated. To verify the difference between the treatments tested in chemical and biological control, the mycelial growth index (MCI) and the percentage of mycelial growth inhibition (ICP) were estimated. In general, the fungicide pyraclostrobin was the most efficient both in vitro and in vivo in the control of Colletotrichum spp. In addition, T. harzianum is a promising species for the reduction of anthracnose of anthracnose-associated species in passion fruit crops. The isolates of B. subtilis (R14 and IN937b) obtained the best results in all Colletotrichum species tested.

4
  • JUTAHY JORGE ELIAS
  •  

     
     
     
     
  • Leader : HENRIQUE FONSECA GOULART
  • MEMBRES DE LA BANQUE :
  • ANTONIO EUZEBIO GOULART SANTANA
  • HENRIQUE FONSECA GOULART
  • JAKELINE MARIA DOS SANTOS
  • ROSEANE CRISTINA PREDES TRINDADE
  • VANDERSON BARBOSA BERNARDO
  • Data: 28 févr. 2024


  • Afficher le Résumé
  •  

     
     
     
     
5
  • ALINE MARQUES MELO
  • BEHAVIOR OF Phaseolus lunatus to Macroptilium yellow spot virus GENOTYPES AND GENETIC DIVERSITY OF BEGOMOVIRUS IN Macroptilium lathyroides
  • Leader : GAUS SILVESTRE DE ANDRADE LIMA
  • MEMBRES DE LA BANQUE :
  • GAUS SILVESTRE DE ANDRADE LIMA
  • GILDEMBERG AMORIM LEAL JUNIOR
  • MARIOTE DOS SANTOS BRITO NETTO
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • MAYRA MACHADO DE MEDEIROS FERRO
  • ROBERTO RAMOS SOBRINHO
  • Data: 29 févr. 2024


  • Afficher le Résumé
  • Begomoviruses (family Geminiviridae) are an important genus of viruses that infect cultivated plants in the most diverse regions of the planet, causing significant economic losses in crop fields. In addition to being found associated with cultivated hosts, these viruses are also associated with a wide range of wild/weed hosts, and may serve as alternative hosts or as a source of inoculum for epidemics in commercial crops. In this context, the present study aims to evaluate the response of different fava bean genotypes to infections caused by the begomovirus Macroptilium yellow spot virus (MaYS) and the vector Bemisia tabaci (Middle East-Asia Minor) and to determine the genetic variability of begomovirus infecting Macroptilium lathyroides. In order to evaluate the variability and genetic structure of begomovirus populations infecting M. lathyroides, leaf samples showing symptoms indicative of begomovirus infection were collected in different municipalities of the State of Alagoas in 2019 and 2020. Total DNA was extracted from each sample and served as a template for rolling circle amplification (RCA), then cloned into plasmid vectors and completely sequenced. A total of 39 clones were obtained (29 DNA-A and 10 DNA-B). Paired DNA-A sequence comparisons showed that all isolates belong to a single species: Macroptilium yellow vein virus (MaYVV). Phylogenetic analyzes showed the division of MaYVV isolates into four subpopulations, which showed low values of nucleotide variability. Recombination analyzes showed evidence of intraspecific recombination events in MaYVV isolates The results obtained suggest that the low genetic variability observed for the isolates may have been generated by possible genetic bottlenecks and that the strong geographical structure of the population shows that there is little interaction between the subpopulations and that they may continue to differentiate. To evaluate the response of different lima bean genotypes to infections caused by the MaYSV begomovirus and the B. tabaci (Middle East-Asia Minor) vector, assays will be carried out to evaluate the different responses of MaYSV infection to lima bean genotypes, to to identify a possible resistance of genotypes to the virus. In addition to tests of attractiveness of the vector B. tabaci to the different fava bean genotypes, to determine a possible preference of the whitefly in relation to the different genotypes. With this work we will be able to obtain important results that may have effects on the longevity of disease management strategies.

     

     

6
  • KEVISON ROMULO DA SILVA FRANÇA
  • Identification of Lasiodiplodia spp. Associated With Crown Rot in Banana Tree in the North and Northeast Regions of Brazil.

  • Leader : IRAILDES PEREIRA ASSUNCAO
  • MEMBRES DE LA BANQUE :
  • FREDERICO MONTEIRO FEIJÓ
  • GILDEMBERG AMORIM LEAL JUNIOR
  • IRAILDES PEREIRA ASSUNCAO
  • JAQUELINE FIGUEREDO DE OLIVEIRA COSTA
  • MAYRA MACHADO DE MEDEIROS FERRO
  • Data: 29 févr. 2024


  • Afficher le Résumé
  • largest cultivated area and, together with the North, accounts for approximately 47% of national production. Although it is a crop of great socio-economic importance, its productivity has been limited by the occurrence of various phytosanitary problems. Crown rot, one of the main post-harvest diseases of banana fruit, causes significant losses during storage, transportation, and marketing. Infections occur during harvest, but symptoms appear in the post-harvest period, during the physiological ripening of the fruit. Different fungi are associated with this disease, which may vary according to geographical region, especially species of the genus Lasiodiplodia. In this context, this study aims to identify Lasiodiplodia species associated with crown rot in banana-growing areas in the North and Northeast of Brazil. In the first chapter, 114 pathogenic isolates of Lasiodiplodia were obtained from commercial plantations in the states of Piauí, Ceará, Rio Grande do Norte, Paraíba, Pernambuco, Alagoas, Sergipe, Bahia, Acre and Pará. Based on morphological, molecular, and phylogenetic analyses, it was possible to identify six species of Lasiodiplodia associated with crown rot disease: L. hormozganensis, L. laeliocattleyae, L. iraniensis, L. pseudotheobromae, L. brasiliensis and L. theobromae. In the second chapter, the sensitivity profile of the six species identified to fungicides with different active ingredients was evaluated. The efficacy of the fungicides in inhibiting mycelial growth varied between species and according to the active ingredient evaluated. Fipronil + Thiophanate-Methyl + Pyraclostrobin, Thiophanate-Methyl and Chlorothalonil showed the highest inhibition values, followed by Difenoconazole, Pyraclostrobin and Azoxystrobin+Difenoconazole.

7
  • VALDEMIR ALBUQUERQUE DA SILVA JÚNIOR
  • DIFFERENTIAL ADAPTABILITY BETWEEN ISOLATES OF bean golden mosaic virus AND Macroptilium yellow spot virus IN LEGUMINOUS HOSTS, AND EVALUATION OF THE LETHAL EFFECT OF ETHANOLIC EXTRACTS OF Annona muricata and A. squamosa ON ADULTS OF Bemisia tabaci

  • Leader : SARAH JACQUELINE CAVALCANTI DA SILVA
  • MEMBRES DE LA BANQUE :
  • AYANE FERNANDA FERREIRA QUADROS
  • ADRIANO MARCIO FREIRE SILVA
  • GAUS SILVESTRE DE ANDRADE LIMA
  • LEONARDO DA FONSECA BARBOSA
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • Data: 29 févr. 2024


  • Afficher le Résumé
  • Among the various phytosanitary problems affecting bean crops, begomoviruses stand out as one of the main concerns. Although several species have been observed naturally infecting these hosts, two viruses in particular, bean golden mosaic virus (BGMV), assume significant economic importance in Brazil. The genus Begomovirus (family Geminiviridae) is characterized by its single-stranded circular DNA genome, encapsulated in geminate quasi-icosahedral particles. They are transmitted in a persistent-circulative manner by insects of the Bemisia spp. complex and have the ability to infect a wide variety of dicotyledonous plants. Macroptilium yellow spot virus (MaYSV), a begomovirus that initially originated in uncultivated legumes, has emerged as a significant pathogen for common beans in the Northeast region of Brazil. The incidence of these begomoviruses in bean crops can cause serious economic losses due to reductions in productivity and grain quality. Therefore, it is crucial to implement integrated pest and disease management measures to mitigate the impacts of these viruses. The aim of this study was to evaluate the ability of infectious clones of BGMV and MaYSV to cause systemic infection in common beans, fava beans, and Macroptilium lathyroides. For this purpose, single and mixed infection assays of the isolates were conducted, as well as transmission assays by the whitefly B. tabaci MEAM1. Based on the results, it was observed that BGMV demonstrated better aptitude in causing systemic infection in all three host plants in both assays. Additionally, the lethal effect of ethanolic extracts of Annona muricata (soursop) and A. squamosa on adult whiteflies was evaluated. The results indicated that both extracts were toxic to the insect, with the soursop extract being more toxic compared to the custard apple extract in all bioassays.

2023
Thèses
1
  • IARA MARIA DOS SANTOS COSTA
  • GENETIC DIVERSITY OF BEAN BEAN-ASSOCIATED BEGOMOVIRUS CULTIVATED IN A FAMILY PRODUCTION SYSTEM IN ALAGOAS

  • Leader : SARAH JACQUELINE CAVALCANTI DA SILVA
  • MEMBRES DE LA BANQUE :
  • GILDEMBERG AMORIM LEAL JUNIOR
  • MAYRA MACHADO DE MEDEIROS FERRO
  • ROBERTO RAMOS SOBRINHO
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • Data: 16 févr. 2023


  • Afficher le Résumé
  • Beans are a staple food of fundamental importance for food and nutritional security as a
    source of protein, in addition to income for small producers in Alagoas. Practically all of
    the bean production in the State of Alagoas comes from family farming, which it uses for
    its own consumption and sells its surplus, supplying the demands of the domestic market.
    Production is based on the cultivation of common bean (P. vulgaris) and broad bean (P.
    lunatus), and to a lesser extent, cowpea (Vigna unguiculata) and pigeonpea (Cajanus
    cajan). The productivity of these crops is considered low, mainly due to the low adoption
    of technology and the occurrence of phytosanitary problems, with emphasis on viruses
    that belong to the genus Begomovirus (Family Geminiviridae). These have limited
    production and reduced grain quality in bean crops grown in all areas of Brazil. Several
    begomoviruses have been reported naturally infecting cultivated and non-cultivated
    legumes, but Bean golden mosaic virus (BGMV) and Macroptilium yellow spot virus
    (MaYSV) are the most economically important in the country. These species occur in
    Alagoas since 2005 in bean and broad bean crops, and non-cultivated plants such as
    Macroptilium spp., with MaYSV being the prevalent virus. In this context, the objective
    of this research is to determine the genetic diversity of begomoviruses associated with P.
    lunatus in a family production system in Alagoas. Leaf samples of P. lunatuss were
    collected in different areas of family cultivation in the state of Alagoas. The total DNA
    of each sample was extracted and used, initially, as a template for detection of
    begomoviruses, using degenerate primers. Subsequently, the detection of the genomic components DNA-A and DNA-A of BGMV and MaYSV was performed using species-specific primers. Samples that tested negative for BGMV and MaYSV begomoviruses had their genomic components amplified via RCA, cleaved with restriction enzymes, ligated into the pBluescript KS+ vector and cloned into Escherichia coli DH10B. Clones containing recombinant vectors were selected for primer walking sequencing. 2 DNA-B clones were obtained and subjected to whole-genome pairwise analysis with otherpreviously reported begomoviruses. These were more closely related to the species BGMV BR:Pai8:11 from Palmeira dos Índios - AL, with 95.72% nucleotide identity. However, it was not possible to correctly attribute taxonomy to the new isolates, due to the absence of DNA-A. The phylogenetic relationship between the characterized isolates was determined by reconstructing Bayesian inference phylogenetic trees for the DNA-A and DNA-B genomic component dataset. Possible recombination events and sites between the begomoviruses found and others stored in databases will also be determined.

2
  • JESSICA MARIANA SILVA COSTA
  • SOURSOUP SEED EMULSIONABLE EXTRACT: POTENTIALITY FOR THE CONTROL OF PLANOCOCCUS CITRI (RISSO, 1813) (HEMIPTERA: PSEUDOCOCCIDAE) IN THE FIELD
  • Leader : ROSEANE CRISTINA PREDES TRINDADE
  • MEMBRES DE LA BANQUE :
  • ROSEANE CRISTINA PREDES TRINDADE
  • MARIANA OLIVEIRA BREDA
  • JOSE DAILSON SILVA DE OLIVEIRA
  • Data: 23 févr. 2023


  • Afficher le Résumé
  • The mealybug, Planococcus citri (RISSO, 1813) (Hemiptera: Coccidae), has a range of hosts, attacking several crops of agricultural and ornamental importance. It is a pest that is found in tropical, subtropical and temperate regions and for its control, there are few insecticides registered for all affected crops. For sugar apple, Annona squamosa L. (Annonaceae), control methods are non-existent. Thus, research focused on control strategies, including applicability in the field with plant extracts, is a very promising area because it has the advantage of not causing damage to humans, the environment, non-target organisms and not selecting resistant populations. Plant extracts, from species of the Anonnaceae family, have been shown in several studies to be a promising alternative for the control of agricultural pests, including showing selectivity to natural enemies. Thus, the objective of this work was to evaluate the toxicity of the emulsifiable extract of soursop seeds, Annona muricata L. (Annnaceae), on the mealy cochineal, in sugar apple fruits, Annona squamosa L. (Anonnaceae), under laboratory and field conditions. with the Verdinha and Crioula varieties. For that, the experiments were carried out in the sugar apple orchard, Annona squamosa L. (Annonaceae), in the experimental area of the Campus of Engineering and Agricultural Sciences of the Federal University of Alagoas. For the formulation of the emulsifiable extract, different amounts of Span® and Tween®, distilled water and ethanol extract of soursop were used. For lethal and residual effect tests, adult females of P. citri were used. Through contact, the lethal concentrations LC50 and LC99 (0.8% and 2.6%) were estimated, using only the LC99 to evaluate the insecticidal action of the emulsifiable extract. The toxicity of the extract in the laboratory caused mortality of 94.71%, while in the field in the Verdinha and Crioula varieties it was 100% and 97.03%, respectively. The residual effect in the laboratory was more effective with 2 hours of exposure with a mortality of 61.39% at the end of the evaluation. For the Verdinha variety, the best exposure time was 24 hours with a mortality of 87.03%, for the Crioula variety the extract was more promising with 48 hours causing a mortality of 70.76%. The emulsifiable formulation has a lethal and residual effect on adult P.citri females. The morphological characteristic of the Verdinha variety provided an increase in the efficiency of the extract on mealybugs.

3
  • MARIA ISABEL GOMES DOS SANTOS
  • RESISTANCE OF YELLOW PASSION FRUIT (Passiflora edulis) GENOTYPES TO ANTHRACNOSE

  • Leader : GILDEMBERG AMORIM LEAL JUNIOR
  • MEMBRES DE LA BANQUE :
  • GILDEMBERG AMORIM LEAL JUNIOR
  • JAQUELINE FIGUEREDO DE OLIVEIRA COSTA
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • Data: 27 févr. 2023


  • Afficher le Résumé
  • The genus Passiflora (family Passifloraceae) occurs mainly in tropical regions. Brazil is considered the most important center of diversity of the genus and the largest producer of passion fruit. Phytosanitary problems limit the expansion of the crop and the maintenance of crops in the same area for a prolonged period. Anthracnose is one of the fungal diseases that threaten the passion fruit production sector. Caused by fungal species of the genus Colletotrichum spp. and in the passion fruit crop, genetic resistance is the best alternative for disease control. However, there is a need to identify the sources of resistance and characterize the accessions for indication in crosses for the development of new varieties. The research aimed to identify accessions of yellow passion fruit (Passiflora edulis) with resistance to anthracnose in the germplasm bank of EMBRAPA Mandioca e Fruticultura Tropical. Colletotrichum spp. were isolated from plants with symptoms of anthracnose in crops from Alagoas and Bahia. The isolates were evaluated for aggressiveness on passion fruit cultivated by farmers. Four isolates were inoculated into 16 passion fruit accessions. When the plants reached 60 days after germination, with 5 to 6 leaves, they were inoculated with a mycelium disc in a lesion made with a sterilized needle on the adaxial surface. The incidence of anthracnose was determined based on the appearance of the first lesions, measuring the diameter of the lesion, the first reading being taken 2 days after inoculation. The collected data were submitted to analysis of variance (ANOVA), and the averages were compared using the Scott-Knott test, at a 5% significance level, using the SISVAR program. Questionnaires were applied to passion fruit producers in Alagoas, in order to analyze the real situation they face for the production and marketing of passion fruit. It was possible to see that family farming in Alagoas is strong and that passion fruit is a well-regarded product for commercialization. However, for producers, improvements are needed in the context in which they live, such as training courses and education in the field of quality. The Gigante Amarelo and HRI30 P.A genotypes were considered less susceptible and more susceptible, respectively, to anthracnose, taking into account that the Gigante Amarelo is an improved variety released as resistant in the market, being one of the most used by passion fruit producers in Alagoas.

4
  • ROSINEIDE DOS SANTOS COSTA
  • Resistance of banana cultivars Musa spp. (Zingiberales: Musaceae) to the mite Raoiella indica Hirst (Acari: Tenuipalpidae)
     
     
     
     
     
  • Leader : EDMILSON SANTOS SILVA
  • MEMBRES DE LA BANQUE :
  • EDMILSON SANTOS SILVA
  • ELIO CESAR GUZZO
  • MARCIA DANIELA DOS SANTOS
  • MARIA DE FATIMA SILVA MUNIZ
  • MÉRCIA ELIAS DUARTE
  • Data: 28 févr. 2023


  • Afficher le Résumé
  • The mite Raoiella indica Hirst, 1924 (Acari: Tenuipalpidae) was originally reported in Brazil in 2009 causing negative impacts on several plant species, among the most important are the families Arecaceae and Musaceae. In Brazil, there are few studies investigating banana tree varieties resistant to the red palm mite. Thus, the present study aims to test six banana cultivars to determine the survival of R. indica and to verify the possible resistance of the cultivars to this mite. At the Laboratory of Entomology and Acarology /LEA at UFAL, Campus Arapiraca, leaf discs of six banana cultivars ('Grand Naine', 'Maçã', 'Prata Anã', 'Prata Catarina' 'Prata Gorutuba' and 'Thap Maeo') (Musa sp.) and coconut Cocos nucifera L. (Arecaceae) (control) were infested with five couples of R. indica and kept in B.O.D, at 25 ± 2 ºC, 57% ± 5 RH and 12h photophase. The evaluations took place daily with verification of the number of live mites and deposited eggs, number of larvae, nymphs and emerged adults. With these data it was possible to calculate the average survival of adults and of females and males separately, in addition to egg viability and survival of immature forms. The design was completely randomized with seven treatments (six banana cultivars + coconut as control) and 10 replications. The results were subjected to analysis of variance and their means compared by the Scott-Knott test using the statistical program AgroEstat ver. 1.1. The maximum longevity of R. indica was observed in the coconut tree at 44 days and at 41 days in the banana cultivar 'Prata Catarina'. The lowest maximum longevity rates were observed in 'Grand Naine' (26 days) and 'Prata Anã' (33 days). Regarding the sex of the mite, regardless of the evaluated cultivar, the females had greater longevity and survived until the 44th day of evaluation. Total oviposition was higher in the control (13.16 eggs) and in 'Thap Maeo' (1.86 eggs), but the latter did not differ from 'Maçã' (1.74%). The lowest oviposition averages occurred in 'Prata Anã' (0.43%) and 'Grand Naine' (0.05%), differing between themselves and between the other banana cultivars and the control. The oviposition period ranged from 21.9 days in the coconut tree to 2.1 days in the 'Grand Naine' cultivar. Considering the viability of the entire development period (egg-larvae-nymph-adult) of R. indica, there was a significant difference between the coconut palm and among all banana cultivars. The survival of the R. indica mite was higher in the 'Prata Catarina' cultivar and in the 'Thap Maeo' cultivar there was the longest oviposition period and the highest average number of eggs. In the 'Grand 'Naine' and 'Maçã' cultivars, the immature forms did not reach the adult stage. The 'Grand Naine' cultivar can be considered resistant to R. indica attack.

     

     

5
  • GESSICA JACIRA TRINDADE DE SOUZA
  • SPECIES OF COLLETOTRICHUM ASSOCIATED WITH BANANA IN THE STATE OF ALAGOAS
  • Leader : IRAILDES PEREIRA ASSUNCAO
  • MEMBRES DE LA BANQUE :
  • IRAILDES PEREIRA ASSUNCAO
  • GILDEMBERG AMORIM LEAL JUNIOR
  • MARIA DE FATIMA SILVA MUNIZ
  • MARIOTE DOS SANTOS BRITO NETTO
  • Data: 28 févr. 2023


  • Afficher le Résumé
  • Brazil annually produces about 6.7 million tons of bananas, the cultivation of banana trees (Musa spp) in the country moved R$ 9.9 million reais in the year 2021, being established, therefore, as an activity of great social importance and economic for the country. The northeast region represents 40% of the Brazilian areas cultivated with bananas and the State of Alagoas plays an active part in this production. However, the average yield of banana production in Alagoas is two tons below the national range, data resulting from limitations generated by diseases in the post-harvest of fruits, such as anthracnose, which is caused by phytopathogens of the genus Colletotrichum, whose infection it is characterized by small lesions that coalesce and form large necrotic areas in the fruit's epidermis, reducing their commercial shelf life by stimulating the early ripening of the fruit, generating losses of up to 40% of production. Therefore, in view of the relevance of investigations regarding the Colletotrichum species associated with banana anthracnose, this study aimed to characterize the distribution, prevalence and resistance of Colletotrichum isolates in the State of Alagoas, based on physiological, morphocultural, pathogenic characteristics and molecular properties of these species. The results obtained here indicate that banana anthracnose in the state of Alagoas is associated with the species Colletotrichum siamense and Colletotrichum musae¸ and the fungicide difenoconazole inhibits up to 85% of the mycelial growth of C.musae.

6
  • GESSIANE MARIA DA SILVA SANTOS
  • COMPONENTS OF SAPROPHYIC ADAPTABILITY OF ISOLATES OF Rhizoctonia solani AG-4 HGI ASSOCIATED WITH BRASSICA HORTALITISTS IN BRAZIL

  • Leader : KAMILA CÂMARA CORREIA
  • MEMBRES DE LA BANQUE :
  • IRAILDES PEREIRA ASSUNCAO
  • KAMILA CÂMARA CORREIA
  • MARISSONIA DE ARAUJO NORONHA
  • Data: 8 mars 2023


  • Afficher le Résumé
  • Rhizoctoniosis is an important disease of vegetable brassicas in Brazil, caused mainly by Rhizoctonia solani AG-4 HGI. Still, so far, there are no studies on the saprophytic adaptability components of the isolates. Therefore, the objective of this dissertation was to compare the saprophytic adaptability of the isolates of R. solani AG-4 HGI associated with vegetable brassicas in Brazil concerning the sensitivity of mycelial growth to different temperatures, pH levels, water potential and salinity, and fungicides. In all experiments, 42 isolates of R. solani obtained from vegetable brassicas with rhizoctoniosis symptoms and belonging to the AG-4 HGI anastomosis group were used. The isolates differed in sensitivity at temperatures of 15, 25 and 35 °C, as well as at pH levels 5, 7, and 9, water potential of -0.8, -1.6, and -3.2 MPa and salinity of 1, 2, and 4%. Most isolates showed greater growth at 25 °C and less at 15 °C. Using Scott-Knott cluster analysis, five groups of isolates were found at 15°C and 25°C, and eight groups at 35°C. Most isolates showed lower growth at pH 9, but 38.1% of isolates were not affected by pH. Six, five, and four groups of isolates were found at pH 5, 7, and 9, respectively. All isolates reduced the growth rate with decreasing water potential, and eight groups of isolates were found at -0.8 MPa and -3.2 MPa, and seven groups at -1.6 MPa. Most isolates reduced growth with increasing salinity level, with five groups of isolates found at 1% and 2% salinity, and four groups at 4% salinity. Most isolates showed sensitivity to fludioxonil, pencycuron and methyl thiophanate fungicides, but with different levels of growth inhibition. Means of inhibition were 99.94%, 91.59% and 12.01% for fludioxonil, pencycuron and methyl thiophanate, respectively. The growth inhibition of 54.8% of the isolates was high and similar with fludioxonil and pencycuron. Only one group of isolates was found when fludioxonil was used, two groups with pencycuron and 11 groups with methyl thiophanate. Multivariate analysis separated the 42 isolates into five groups. The R. solani AG-4 HGI isolates, despite belonging to the same anastomosis group, are separated into different similarity groups based on saprophytic adaptability, indicating the need for this variability to be considered in the development of management strategies for rhizoctoniosis in vegetable brassicas in Brazil.

7
  • RAFAEL DE ALMEIDA LEITE
  • SUSCEPTIBILITY OF DWARF COCONUT PALM CULTIVARS TO THE RED PALM MITE.

  • Leader : ELIO CESAR GUZZO
  • MEMBRES DE LA BANQUE :
  • ELIO CESAR GUZZO
  • EDMILSON SANTOS SILVA
  • MARIANA OLIVEIRA BREDA
  • ANIBAL RAMADAN OLIVEIRA
  • ADENIR VIEIRA TEODORO
  • Data: 28 juin 2023


  • Afficher le Résumé
  • The red palm mite Raoiella indica Hirst, 1924 (Acari: Tenuipalpidae) is an important pest of the coconut palm Cocos nucifera L. (Arecaceae) and has caused problems in coconut production due to its ease of dispersion and insufficient registered products to control it. Research has continued since its arrival on the American continent, aiming to control the mite through chemical, biological, alternative methods and through resistant plants. Resistant plants are a great alternative to reduce problems caused by pests. Therefore, the objective of this work was to evaluate the performance of R. indica in six dwarf coconut cultivars, in order to identify sources of resistance of these cultivars to the mite. The performance of R. indica was evaluated on the cultivars Green Dwarf from Brazil from Jiqui (AVeBrJ), Red Dwarf from Cameroon (AVC), Red Dwarf from Malaysia (AVM), Red Dwarf from Gramame (AVG), Yellow Dwarf from Gramame (AAG) and Yellow Dwarf from Malaysia (AAM), through confinement and free-choice tests, evaluating longevity, pre-oviposition and oviposition periods, fertility, fecundity, viability of life stages and choice for feeding and oviposition. The AVeBrJ cultivar was the most resistant to R. indica, providing the lowest adult survival, oviposition period, number of eggs, egg viability, fecundity and fertility, in addition to being less preferred in relation to AVM and AAM in the free-choice test. The resistance of this cultivar is probably related to some chemical factor.

8
  • LILIAN RENATA ALVES FARIAS
  • ECPROTOCOL FOR TOXICOLOGICAL ANALYSIS OF BOTANICAL INSECTICIDES: EFFECTS OF THE MICROENCAPSULATED FORMULATION OF THE ETHANOL EXTRACT OF Annona muricata L. (ANNONACEAE) ON NILE TILAPIA
  • Leader : ROSEANE CRISTINA PREDES TRINDADE
  • MEMBRES DE LA BANQUE :
  • ROSEANE CRISTINA PREDES TRINDADE
  • MARIANA OLIVEIRA BREDA
  • JERUSA MARIA DE OLIVEIRA AMORIM
  • Data: 9 août 2023


  • Afficher le Résumé
  • Toxicological studies have become more and more frequent due to the growing concern about the effects on the environment and on non-target organisms. This increased attention is also related to the increased adoption of alternatives, such as the use of botanical insecticides in agriculture. This concern is accentuated by the absence of specific legislation that establishes parameters for the use of these products, which implies the lack of defined toxicological limits. In view of this, the objective was to evaluate possible toxic effects of the microencapsulated formulation of ethanolic extract of Annona muricata L (Annonaceae), a product with a patent already granted (BR102018008313-9), on fingerlings of Nile tilapia, Oreochromis niloticus (Linnaeus, 1758) (Cichliformes: Cichlidae). Acute and chronic toxicity tests were performed, with tilapia fingerlings approximately 30 days old with measuring on average between 1.5 and 3.0 cm, which were exposed to concentrations to increasing concentrations 0.125; 0.250; 0.500; 1.0 and 2.0 g/L of the formulation for 24 and 96 hours. As well as biochemical parameters with antioxidant enzymes. Based on the mortality results, the concentration values that kill 50% of the evaluated population (CL50), obtained by Probit analysis by SAS, resulting in 0.229 and 0.249 g/ L in 24 and 96 hours, respectively. The enzymatic activity of superoxide dismutase (SOD) and glutathione S-transferase (GST) was quantified in the liver and gills and acetylcholinesterase (AChE) was quantified in the brain of O. niloticus. ANOVA statistical analysis showed differences only for SOD and GST activities in the liver. Despite that, when individuals were exposed to the two highest concentrations 1.0 and 2.0 g/L, they showed mortality rates of 93.33 and 100%, respectively, after 24 hours of exposure. Mortality likely stemmed from disturbance of the animal and alterations to its natural habitat. This is because the results of enzymatic activity and Acetyl analysis were positive when compared to the estimated LC50 value. Therefore, since toxicology is a weakness in the promotion of alternative products, more studies are needed in the area of natural products, such as extracts and oils.

9
  • THAYNNARA PAULA DOS SANTOS LIRA
  • PROSPECTING RESISTANCE TO Colletotrichum truncatum IN THE GENETIC DIVERSITY OF THE CREOLE VARIETY OF Phaseolus lunatus

  • Leader : GILDEMBERG AMORIM LEAL JUNIOR
  • MEMBRES DE LA BANQUE :
  • GILDEMBERG AMORIM LEAL JUNIOR
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • MARIA DE FATIMA SILVA MUNIZ
  • Data: 18 août 2023


  • Afficher le Résumé
  • The cultivation of Fava bean (Phaseolus lunatus L.) is typically conducted by family farmers and represents a crop of significant economic value in the Northeast region. However, substantial losses in the crop have been common due to the action of phytopathogenic fungi. Fungi of the Colletotrichum genus cause anthracnose, a disease that affects various plant species, including Fava bean. Genetic resistance presents itself as a sustainable and low-cost alternative for disease control. However, there is a need to identify sources of resistance and characterize them in order to perform crosses and develop new varieties. The research aimed to identify the ethnovarieties of Fava bean present in the municipality of São Sebastião/AL, ascertain whether the producers possess ancestral knowledge and emotional connections with the cultivated varieties, and evaluate resistance to anthracnose using the Colletotrichum truncatum (ICT12) isolate. Predefined questionnaires were used to interview Fava bean producers in the region to record cultivation conditions and the history of collected materials. Thirty-seven seed samples were acquired, identified, and stored at the Phytopathological Clinic of the Campus of Engineering and Agricultural Sciences (CECA) of UFAL. The varieties were subjected to pathogenicity tests in a controlled environment and on detached leaves through inoculation of the isolate on the upper surface of the leaves. Among the interviewed producers, the majority (45.4%) had over 30 years of cultivation experience. Additionally, 63.3% stated that they obtained the seeds from their ancestors. Regarding cultivation practices, 54.4% of the producers usually intercrop Fava beans with corn and store the seeds in plastic bottles (PET). The use of fertilizer is the primary management method for 90.9% of the respondents. Agriculture is the sole source of income for 81.8% of them, and they do not require assistance in cultivating Fava beans. In terms of consumption and commercialization, 63.6% of the producers stated that they consume the Fava beans they produce, while 36.3% not only consume but also sell them. In the in vivo and in vitro evaluations, the PLI and PLGB varieties showed high resistance, while the PLLS5 variety demonstrated high susceptibility. The producers possess ancestral knowledge and emotional connections with the cultivated varieties of Fava bean, which show great promise for studies on genetic resistance to pathogens.

Thèses
1
  • EDIVÂNIA MATIAS DA SILVA
  •  

    LETHAL AND SUBLETAL EFFECTS OF NAPHTOQUINONE ANALOGS AGAINST Mononychellus tanajoa (Bondar, 1938) (ACARI: TETRANYCHIDAE), PEST PRESENT IN CASSU (Manihot esculenta Crantz).

     

  • Leader : EDMILSON SANTOS SILVA
  • MEMBRES DE LA BANQUE :
  • ADEILDO JUNIOR DE OLIVEIRA
  • EDMILSON SANTOS SILVA
  • JANDIR CRUZ SANTOS
  • MÉRCIA ELIAS DUARTE
  • ROSEANE CRISTINA PREDES TRINDADE
  • Data: 16 févr. 2023


  • Afficher le Résumé
  • The cassava green mite, Mononychellus tanajoa (Bondar 1938) (Acari: Tetranychidae), is of great importance and can cause considerable losses in cassava crops. The control of the green mite, M. tanajoa is difficult, since until now there are no acaricides registered for this pest in the cassava crop. Natural and synthetic naphthoquinone compounds stand out for having varied biological activities, including acaricides. Therefore, the present work aimed to analyze the lethal and sublethal effects related to the repellent effect, instantaneous population growth rate and residual effect of natural and synthetic naphthoquinone compounds on the green mite, M. tanajoa in the cassava crop. Lethal concentrations of naphthoquinones were estimated by Probit analysis. To evaluate the repellent effect, cassava leaf discs were sprayed with water + 2%DMSO (control) or with the treatments (CLs 5, 25, 50, 75, 90 and 95) of the nine naphthoquinone compounds. Then, they were placed in a Petri dish and connected by a coverslip, with the treated leaf disc on one side and the control on the other. In the center of the coverslip 10 females of M. tanajoa were released and after 24 hours the number of females in each disc was evaluated, 15 repetitions were performed per treatment. For the instantaneous population growth rate (ri), cassava leaf discs were immersed with the compounds (CLs 5, 25, 50, 75, 90 and 95) and the control. Five females were transferred per disc and ten replicates were used per treatment. After 10 days, the total number of mites was counted. To evaluate the residual effect, cassava seedlings were sprayed with the LC95 of the compounds. After 2 h of application and during the periods of 1, 2, 4, 8 and 16 days after application, leaf discs were removed, placed in Petri dishes and transferred 10 females of M. tanajoa, the mortality assessment was 72 h after the application. lockdown. Among the naphthoquinones, the derivative 2-(4-((5-Methyloxazol-3-yl)-methylsulfonyl)-phenylamino)-naphthalene-1,4-dione) (C7) was the most toxic, followed by ((4 -Pyridin-3-yl)-methylsulfonyl)-phenylamino)-naphthalene-1,4-dione (C6), and by 2-bromo-naphthalene-1,4-dione (C4), with LC50 values (IC 95%) of 3.08; 5.29 and 6.33 µL/mL and LC90 (95% CI) 16.21; 24.53 and 34.31 µL/mL, respectively. Therefore, the following order of acaricidal activity was observed against the green mite, M. Tanajoa C7 > C6 > C4 > C2 > C3 > C1 > C8 > C9 > C5 for both LC50 and LC90. The viability of M. tanajoa eggs was affected after the application of Naphthoquinones (C1-C9). Based on LC50 and LC90 values 2-(4-((5-Methyloxazol-3-yl)-methylsulfonyl)-phenylamino)-naphthalene-1,4-dione) (C7) was the most toxic, followed by ( (4-Pyridin-3-yl)-methylsulfonyl)-phenylamino)-naphthalene-1,4-dione (C6), 2-bromo-naphthalene-1,4-dione (C4) and by (N'-(1, 4-Dihydro-1,4-dioxonaphthalen-2-yl)-isonicotinoylhydrazide) (C8). For these compounds, interference in the viability of eggs was observed, as well as which were the smallest amounts of the product to cause 50% and 90% of mortality in the population with estimated LC50 values of 5.77, 6.15, 6.62 and 8.16 µg/mL, and LC 90 with values of 23.77; 18.41; 20.38 and 31.55, respectively. Therefore, the following order of acaricidal activity was observed in eggs of the green mite, M. Tanajoa C7 > C6 > C4 > C8 > C9 > C5 > C1 > C2 > C3. Our studies showed that C7, C6 and C4 showed efficient results regarding the toxicity of eggs and adults of M. tanajoa. The structure-activity relationships between the most promising compounds show that the presence of sulfonamide-type substituents linked to position 2 of the quinone nucleus (C7 and C6) allowed an increase in activity by 5.5 and 3.2 times, respectively, compared to 1,4-naphthoquinone itself (C1). On the other hand, the inclusion of the bromine atom at position 2 of 1,4-naphthoquinone was able to increase the activity by 2.7 times compared to C1. In addition, C8 (N'-(1,4-Dihydro-1,4-dioxonaphthalen-2-yl)-isonicotinoylhydrazide) and C9 (N-Isonicotinoylhydrazone-[2-hydroxy-3-(3-methyl-2-butenyl) )]-1,4-naphthoquinone) showed that the change of the hydroxyl group or one of the carbonyl groups of the basic nucleus of naphthoquinones (C2 and C3 compounds) by a polytrogenated group, be it a 2-N-acylhydrazide (C8) or a 1-N-acylhydrazone (C9) contributed to increase the toxicity of the compounds in the eggs in relation to the adult phase of M. Tanajoa, with the presence of the 2-N-acylhydrazone group being more efficient than 1-N-acylhydrazone. The ADME in silico properties of naphthoquinone compounds showed potential to develop as good candidates for acaricides, following Lipinski's rule of five. Repellent effect was observed for natural naphthoquinones, lausone (C2) and lapachol (C3) only from CLs 90 and 95 respectively. However, for C8 and C9 naphthoquinones synthesized from natural compounds, repellency was observed from LC25. In relation to the other synthetic naphthoquinones 1,4 Naphthoquinone (C1), 2-bromo-1,4-naphthoquinone (C4), 2, 3-Dichloro-1, 4-naphthoquinone (C5), 4-Pyridin-3-Il) (Methylsulfonyl) Phenylamino) Naphthalene 1,4-Dione (C6) and 2-(4-((5-Methyloxazol-3-yl) Methylsulfonyl) Phenylamino) Naphthalene-1,4-Dione) (C7) repellent effect was observed M. tanajoa when using contractions referring to CLs 75, 90 and 95. The instantaneous population rate for all compounds decreased with increasing concentrations. Compounds C5 in LC 90 and 95 and compound C7 in LC 95 were able to stabilize the population of M. tanajoa (ri=0). None of the natural compounds C2 (lausone) and C3 (lapachol) tested caused the instantaneous rate of population growth to be negative, however, there was a proportional reduction in the reproduction of mites, with increasing concentrations used. However, compounds C8 and C9 that were synthesized from lausanne and lapachol, respectively, were able to negatively destabilize the population of M. tanajoa. In the residual effect, it was observed that only C3 ([2-Hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthatoquinone - Lapachol) showed moderate residual persistence (mortality ≥ 33% up to 16 days after the application of the products) and the naphthoquinone compounds C1, C2, C4, C5, C6, C7, C8 and C9 show slight persistence (mortality ≥ 27% up to 8 days after the application of the compounds). Naphthoquinones may have potential for the development of acaricides. However, further studies should be carried out to assess the expense and efficiency of these naphthoquinones on a wide variety of pests in the field.

     

     

     

     

2
  • MARIA JUSSARA DOS SANTOS DA SILVA
  • Characterization and alternative control of Fusarium species

    associated with Damping-off in coriander

  • Leader : GAUS SILVESTRE DE ANDRADE LIMA
  • MEMBRES DE LA BANQUE :
  • EDNA PEIXOTO DA ROCHA AMORIM
  • GAUS SILVESTRE DE ANDRADE LIMA
  • JAQUELINE FIGUEREDO DE OLIVEIRA COSTA
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • WELLINGTON COSTA DA SILVA
  • Data: 17 févr. 2023


  • Afficher le Résumé
  • Coriander is a vegetable that has its cultivation consolidated among rural
    producers in northeastern Brazil. However, the incidence of disease
    caused mainly by pathogens transmitted by the soil, becomes one of the
    limiting factors for production. Some problems caused by these
    phytopathogens is the reduction of the stand, due to the damping off of
    seedlings. There are still no concrete studies on the specific cause of the
    disease in culture. However, knowing the etiological agent is essential for
    the development of control methods. Therefore, the objective of this work
    was to identify and characterize phytopathogenic agents associated with
    damping-off of coriander seedlings in the states of Alagoas, Pernambuco
    and Ceará, and to evaluate their in vitro sensitivity to essential oils of
    clove (Eugenia caryophyllus), cinnamon (Cinnamomum cassia),
    citronella (Cymbopogon winterianus) and melaleuca (Melaleuca
    alternifolia). Soils were collected in different coriander producing areas,
    taken to the laboratory where they were distributed in 300 vases with a
    capacity of 250mL and identified according to the municipality of
    collection. To obtain the isolates, six coriander seeds of the Verdão
    cultivar (Isla seeds LTDA) were sown in each pot. Seed germination
    occurred seven days after planting and, 10 days after germination, the
    seedlings showed symptoms of wilting followed by damping off, thus
    performing the indirect isolation of pathogens. In the test to assess
    sensitivity F. fabacearum and F. falciforme, the experimental design was
    completely randomized, in a 4x6 factorial scheme (4 essential oils x 6
    concentrations), with five replications, with the experimental unit
    consisting of a Petri dish. Treatments were generated by combining
    concentrations (0; 12.2; 25; 50; 100 and 200 μL mL-1) of essential oils.
    The plates were inoculated with the pathogens for seven days at 25 ± 2
    °C. To verify the difference between treatments, the mycelial growth
    velocity index (IVCM) was calculated. Using morphocultural and
    molecular analyzes with specific primers for the RPB2 and TEF genes, a 

    total of thirty-eight Fusarium isolates were obtained. Of these, thirty
    isolates clustered in the F. oxysporum complex (one isolate of F. elaeidis;
    one isolate of F. gossypinum and twenty-eight isolates of F. fabacearum)
    and eight isolates in the F. solani complex, together with the species F.
    sickle cell. In the sensitivity test, cinnamon essential oil was the one that
    showed the best result from the concentration of 50 μL mL- with
    fungitoxic action on mycelial growth for the species F. fabacearum and F.
    falciforme when compared to the other oils. Based on this, this is the first
    report of F. fabacearum, F. gossypinum and F. elaidis causing damping
    off in coriander seedlings in the world and cinnamon essential oil shows
    promise for the alternative control of damping off in coriander crops.

3
  • PAULO HENRIQUE TAVARES SANTOS FARIAS
  • Resistance evaluation in accessions of the complex Saccharum spp. to the small borer Diatraea saccharalis (Fab. 1794) (Lepidoptera: Crambidae)

  • Leader : ELIO CESAR GUZZO
  • MEMBRES DE LA BANQUE :
  • ELIO CESAR GUZZO
  • MARIANA OLIVEIRA BREDA
  • ALICE MARIA NASCIMENTO DE ARAUJO
  • ALDOMARIO SANTO NEGRISOLI JUNIOR
  • JOÃO GOMES DA COSTA
  • Data: 24 févr. 2023


  • Afficher le Résumé
  • The search for renewable energies has transformed the current agricultural scenario and, as a result, much emphasis is given to the sugarcane crop, which, in practically all its phenology, is infested by borers, which are key pests for the culture. Thus, researches that corroborate with varieties resistant to these insects become of fundamental importance for their management in production systems. The present study aims to characterize genotypes of the Saccharum spp. and biotic stresses caused by borers. Therefore, we evaluated the intensity of infestation of the giant borer Telchin licus (Drury, 1773) (Lepidoptera: Castniidae) in a commercial area in São Miguel dos Campos, AL during the 2021 and 2022 harvest, analyzing the total amount of internodes and the number of brocade internodes. The larval antiobiosis of D. saccharalis (Fab. 1794) (Lepidoptera: Crambidae) was evaluated from an experimental planting of the genotypes in a greenhouse at the Research Execution Unit of Embrapa Tabuleiros Costeiros (UEP de Rio Largo, AL). In the laboratory, two experiments were carried out: the biology and construction of the fertility life table of D. saccharalis, fed with an artificial diet, produced from the studied sugarcane genotypes and the biology of its parasitoid Cotesia flavipes Cameron (Hymenoptera: Braconnidae), from the parasitism of caterpillars fed the aforementioned diets.

4
  • TACIANA FERREIRA DOS SANTOS
  • M
    MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF FUNGAL SPECIES ASSOCIATED WITH PLANTS OF THE ORCHIDACEAE FAMILY
  • Leader : IRAILDES PEREIRA ASSUNCAO
  • MEMBRES DE LA BANQUE :
  • ANA FRANCISCA TIBÚRCIA AMORIM FERREIRA E FERREIRA
  • IRAILDES PEREIRA ASSUNCAO
  • JAQUELINE FIGUEREDO DE OLIVEIRA COSTA
  • MARIA DE FATIMA SILVA MUNIZ
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • Data: 24 févr. 2023


  • Afficher le Résumé
  • Floriculture is one of the fastest growing agribusiness segments in Brazil, especially the tropical flower market, which is favored by the climatic conditions of the different regions of the country, with emphasis on the northeast region. Within this sector, the production of orchids represents a type of trade in increasing expansion in the national market and this is due to the diversity, beauty of its flowers and its easy cultivation. However, despite the progress, orchid culture has been limited by several factors, among which is the occurrence of diseases, especially those of fungal origin. Knowing the etiological agent is essential for the success, durability and implementation of control measures. Therefore, the objective of the present work was to identify species of fungi associated with orchids through morphocultural and phylogenetic characteristics. The isolates were obtained from native orchids or from commercial plantations showing typical symptoms of diseases with fungal etiology. Subsequently, morphological and cultural studies and pathogenicity tests were carried out in asymptomatic plants belonging to different genera of the Orchidaceae family, followed by DNA extraction and amplification of partial sequences of the glyceraldehyde3-phosphate dehydrogenase (GAPDH) genes. , translation elongation factor 1-α (TEF-1α) and second major RNA polymerase II subunit (RPB2). A total of 80 isolates were obtained, 57 belonging to the genus Colletotrichum, 12 to Fusarium and 11 to pestalotioid fungi. Of these, 11 isolates of Colletotrichum, 5 of Fusarium and 5 of pestalotioid fungi were destined for phylogenetic studies. Colletotrichum cultures showed mycelium varying between shades of gray, yellow and white, with hyaline, non-septate, straight-cylindrical conidia and rounded ends. The Fusarium isolates produced mycelium in shades of white, pink or purple, with hyaline macro and microconidia. While the pestalotioid fungi formed purely white colonies or with an orange reverse, cottony and conidia presenting 2 to 3 apical appendages and a basal appendage. The observation of the morphological and cultural characteristics, together with the phylogenetic analysis of the GAPDH gene, could group the Colletotrichum isolates into the complexes C. gloeosporioides, C. orquidearum, C. gigasporum and C. dracaenophilum; The Fusarium isolates were grouped with species of the F. incarnatum–equiseti complex, F. fujikuroi and F. oxysporum, based on the phylogeny of the partial sequences of the TEF-1α and RPB2 genes. While the pestalotioid fungi clustered with Neopestalotiopsis and Pseudopestalotiopsis species through TEF-1α phylogenetic analysis.

5
  • TIAGO SILVA LIMA
  • IDENTIFICATION OF Colletotrichum spp. ASSOCIATED WITH ANTHRACNOSIS IN HELICONIA AND ASSESSMENT OF SENSITIVITY TO FUNGICIDES.

  • Leader : IRAILDES PEREIRA ASSUNCAO
  • MEMBRES DE LA BANQUE :
  • IRAILDES PEREIRA ASSUNCAO
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • EDNA PEIXOTO DA ROCHA AMORIM
  • MARIA DE FATIMA SILVA MUNIZ
  • JULIANA PAIVA CARNAUBA
  • Data: 24 févr. 2023


  • Afficher le Résumé
  • Diseases are an important obstacle faced by heliconia producers, causing significant losses. The anthracnose disease, for example, is caused by fungi of the genus Colletotrichum, responsible for causing damage due to the difficult control, since the symptoms make the flower stems unsuitable for commercialization. Considered as an important plant pathogen, the genus Colletotrichum has a wide diversity of species, being responsible for causing a variety of diseases in several economic crops and ornamental plants, making it difficult to control this disease. The understanding and knowledge of which species of Colletotrichum are occurring in heliconia, as well as the infectious behavior of these species in different hosts and the effect of different fungicides on them is important for the development of more efficient control strategies. However, identification studies of this genus in heliconias are still scarce. Therefore, the objective of this work was to identify species of the genus Colletotrichum associated with heliconias through the use of molecular tools and morphocultural characteristics, as well as to evaluate the effect of different fungicides on mycelial growth and conidia germination. Fungal isolates were obtained from heliconia plants with anthracnose symptoms, in the states of Alagoas, Ceará and Pernambuco. These isolates will be characterized through multi-locus phylogenetic analysis and mold-cultural characters of the species. In vitro tests with the fungicides (Azoxistrobina + difenoconazol), (Clorotalonil), (Fluxapiroxade + Piraclostrobina) and (Difenoconazole) will be carried out to evaluate the inhibition of mycelial growth by the method of incorporation of fungicides in the PDA culture medium and the inhibition of conidia germination using excavated slides containing water with fungicide. Thirty-two isolates were obtained so far from flowers, leaves, petioles and bracts showing typical symptoms of anthracnose. The initial analysis of the partial sequences of the GAPDH gene was performed with seventeen isolates of Colletotrichum, the sequences were compared with the accesses of the GenBank database using the BLASTn algorithm, together with the construction of Bayesian Inference trees, it was possible to group the isolates in four complexes, two isolated in the C. boninense complex, twelve in the C. gloeosporioides complex, two in the C. magnum complex and one in the C. gigasporum complex. All isolates were able to develop symptoms on inoculation with wounds, inducing depressed, waterlogged, light brown, and variable sized necrotic lesions on leaves. With the study it is expected to know the diversity of species of Colletotrichum associated with the heliconia culture, obtaining relevant information about the pathosystem for the development of adequate strategies for the control of the disease.

6
  • JOSÉ TOMÁZ FERREIRA NUNES
  • ENDOPHYTIC ISOLATES IN GENOTYPES OF Phaseolus lunatus L.

  • Leader : GILDEMBERG AMORIM LEAL JUNIOR
  • MEMBRES DE LA BANQUE :
  • EDNA PEIXOTO DA ROCHA AMORIM
  • GILDEMBERG AMORIM LEAL JUNIOR
  • JOÃO MANOEL DA SILVA
  • MAYRA MACHADO DE MEDEIROS FERRO
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • Data: 27 févr. 2023


  • Afficher le Résumé
  • Even with some inconsistency among the authors about the concept that best characterizes an endophytic, the most usual is that endophytic fungi are those that live, in at least part of their life cycle, inside plant tissues, without apparently causing any harm to their hosts. These definitions began to be discussed with the mycologist De Bary in 1866, but further studies of these organisms did not begin until about a hundred years later, around 1970. Currently, many aspects of endophytic fungi are well established, but there is still a vast field to be explored. Understanding this complex relationship between endophytic and host is fundamental so that, possibly, we can use these organisms in our favor in different fields of science, such as, for example, in the biological control of anthracnose in fava beans. The objectives proposed here aimed to contribute to the advancement of knowledge about the interaction between the lima bean crop and its endophytic fungi, providing opportunities for future biotechnological applications of endophytic fungi in a perspective of developing products and biotechnological processes of interest to agriculture. In the present study, cultivable fungi isolates, which are likely to be beneficial for plant growth, health, and productivity, will be identified, and whether these endophytes vary between plant organs and broad bean genotypes from an Andean and Mesoamerican material, also contrasting for resistance to anthracnose. The study will gather basic information on the occurrence and diversity of endophytic fungal species in new and old leaves, flowers, and fruits of fava genotypes. The isolation and identification of cultivable endophytes may contribute to future studies of the function and application of endophytic fungi in resistance to biotic and abiotic stresses. (AZAD and KAMINSKY, 2016; ABDEL-MONAIN et al., 2014; BERG et al., 2014). Aiding in the development of new, efficient, and sustainable strategies to improve the quality and productivity of crops, while reducing the application of harmful chemicals. In article I, “Diversity of endophytic fungi in Phaseolus lunatus”, the composition and evolution of endophytic fungi during the vegetative and reproductive phases of the host in distinct and contrasting gene pools regarding resistance to anthracnose is presented. Endophytes were isolated from asymptomatic without disease symptoms, namely: new leaves, old leaves, flowers, and pods. With the morphocultural and molecular characterization, the isolates were described until their respective genera. And in Article II, “Prevalence of Colletotrichum ssp. endophytic in broad bean”, a phylogenetic evaluation of endophytic fungi of the genus Colletotrichum is described, and the use of different genomic regions is proposed to evaluate the genetic variability of fungi of this genus.

7
  • JOAIS JOSÉ DA SILVA
  • RESISTANCE INDUCTION BY INOCULATION OF THE FUNGUS Cunninghamella elegans AND INFLUENCE OF NITROGEN FERTILIZATION ON RESISTANCE OF Phaseoulus lunatus To Colletotrichum truncatum

  • Leader : GILDEMBERG AMORIM LEAL JUNIOR
  • MEMBRES DE LA BANQUE :
  • JAMILLY ALVES DE BARROS
  • GILDEMBERG AMORIM LEAL JUNIOR
  • JAQUELINE FIGUEREDO DE OLIVEIRA COSTA
  • JULIO ALVES CARDOSO FILHO
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • Data: 28 févr. 2023


  • Afficher le Résumé
  • The fava beans, or fava beans, originates in Guatemala in Central America, being cultivated mostly by small producers. It is the second legume of greatest socioeconomic importance due to its food properties considered beneficial to human health, being consumed in the form of ripe or green grains. However, diseases such as anthracnose (Colletotrichum spp.) can cause a considerable reduction in production, which causes both direct and indirect damages during the biological cycle of the crop. Most of the time, phytosanitary chemicals are used for its control. And the use of phytosanitary chemicals, provides adverse effects such as the development of resistant populations, outbreaks of secondary diseases, water and soil contamination, negative impact on biodiversity. Another problem is nutritional imbalance. Mineral nutrients can affect plant growth and production, as they are responsible for specific functions in metabolism. And an adequate and balanced nutritional fertilization of nutrients can increase the resistance of plants to diseases. The nutrient most required by plants is nitrogen, which must be associated with proteins and lipids. The objectives of this work are to evaluate the effect of resistance induction by inoculation of the fungus C. elegans, in comparison with applications of commercial chitosan and Ecolife® on the severity of anthracnose in fava beans. And to evaluate the influence of nitrogen fertilization on the resistance of P. lunatus to C. truncatum. Experiments were carried out with the application of the inducers via soil and via leaf. And two experiments, one to evaluate the effect of the dose and the other to evaluate the effect of the source of nitrogen fertilization on the severity of anthracnose in fava beans. Data will be submitted to analysis of variance (ANOVA), and when significant will be submitted to comparison of means by the Scott-Knott test at 5% error probability, with the statistical analysis program SISVAR.

8
  • ELMADÃ PEREIRA GONZAGA
  • STUDY OF THE SEXUAL PHEROMONE OF Thyrinteina arnobia (STOLL, 1782) (LEPIDOPTERA: GEOMETRIDAE) IN EUCALYPTUS

  • Leader : MARIANA OLIVEIRA BREDA
  • MEMBRES DE LA BANQUE :
  • ALICE MARIA NASCIMENTO DE ARAUJO
  • HENRIQUE FONSECA GOULART
  • ISIS TORRES SOUZA
  • JAKELINE MARIA DOS SANTOS
  • MARIANA OLIVEIRA BREDA
  • ROSEANE CRISTINA PREDES TRINDADE
  • Data: 28 févr. 2023


  • Afficher le Résumé
  • The genus Eucalyptus has about 600 species, adapted to the most varied climates and soils. In Brazil, around 7.47 million hectares are occupied by eucalyptus forest stands. With the advance of eucalyptus cultivation in Brazil, the incidence of population outbreaks of insects has been favored. Among the insect pests of the crop, the defoliator caterpillar Thyrinteina arnobia (Stoll, 1782) (Lepidoptera: Geometridae) stands out, cited as the most important lepidopteran for eucalyptus cultivation in the country. In the state of Alagoas, the presence of T. arnobia was found to cause significant damage. Due to the large extension of the eucalyptus forest stands and the height of the trees, the chemical control of T. arnobia becomes economically unsustainable. As a result, new control methods have been proposed, including behavioral control, through the study of the sexual pheromone of T. arnobia. Therefore, it is necessary to understand the mechanisms involved in the chemical ecology of the species, from the sexual pheromone release process, through the perception of these compounds, excitation and response to pheromonal substances. Thus, the present study aims to: (i) provide basic and complementary information on the sexual behavior of T. arnobia, based on the observation of the calling behavior; (ii) carry out the study of antennal sensilla in males and females of T. arnobia, through scanning electron microscopy (SEM); (iii) isolate and identify possible pheromonal substances from extracts of abdominal glands of T. arnobia females, using gas chromatography with flame ionization detector (GC-FID); (iv) and verify the bioactivity of the extracts, through electroantenography coupled to gas chromatography (EAG-GC). Regarding calling behavior, gland exposure occurred after the 2nd hour of scotophase. The calling behavior was characterized by the exposure of the pheromone gland of the females of T. arnobia, the dissemination of the sexual pheromone, the perception of the pheromone by the males and the approach of the males to the emitting source of the pheromone. In total, six types of sensilla were identified in antennae of females and males of T. arnobia, characterized in trichoid, squamiform, caetic, basiconic, celoconic and styloconic. The antennae of female and male T. arnobia have different structural morphology of sensilla. Antennae of both sexes have Böhm's bristles at the base of the scape. The trichoid sensilla are the most abundant, mainly in the antennae of T. arnobia males, indicating that they can respond to stimuli from chemical substances. In the chromatographic profile of the extract of abdominal glands in T. arnobia females from Alagoas, it was possible to previously identify 19 possible compounds. Regarding the bioactivity of pheromonal substances from extracts of abdominal glands of T. arnobia females, it was observed that the electroantennary responses of male antennae submitted to the pheromonal extract of T. arnobia were significant when compared to the hexane stimulus (control).

9
  • MARIA HELLOÁ COSTA DE OLIVEIRA
  • NEXT GENERATION SEQUENCING IN DETERMINING THE DIVERSITY OF BADNAVIRUS THAT INFECT TROPICAL FLOWERS IN THE NORTHEAST REGION

  • Leader : SARAH JACQUELINE CAVALCANTI DA SILVA
  • MEMBRES DE LA BANQUE :
  • GAUS SILVESTRE DE ANDRADE LIMA
  • GILDEMBERG AMORIM LEAL JUNIOR
  • JOÃO MANOEL DA SILVA
  • MAYRA MACHADO DE MEDEIROS FERRO
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • Data: 31 juil. 2023


  • Afficher le Résumé
  • One of the most promising segments of the national agribusiness is floriculture, with an emerging market of profitability all over the world. Tropical flowers are attacked by different pathogens, including badnaviruses. Badnaviruses (Family Caulimoviridae) consist of a group of viruses with a double circular DNA genome, encapsulated in non-enveloped bacilliform particles, and represented mainly by mealybug strands. Diseases dangerous to the world by serious viruses are in serious genera in tropical crops important in tropical ones, including Brazil. To characterize badnavirus species that occur in tropical plants of the Cannaceae, Heliconiaceae, and Zingiberaceae families, they were collected in the poles that produce tropical plants in Alagoas, Pernambuco and Ceará. Subjected to badnavirus detection via PCR) using the universal pink primers for the genus, twelve samples (“Cost. by virus in the invalid virus infection. Once identified as infected with the virus in the episomal form, the RCA products were selected and will be sequenced by NGS. How SDT sequences and genome comparisons sequences will be approved and viral for sequence analysis and SDT genome comparisons for species demarcation. To confirm the presence of infections identified by the NGS and ensure the safety of the method, design PCR primers for each virus designed for full signatures for the full genome via primer walking sequencing.

10
  • CAMILA ALEXANDRE CAVALCANTE DE ALMEIDA
  • BASES FOR THE IMPLEMENTATION OF BIOLOGICAL CONTROL OF Thyrinteina arnobia (Stoll, 1782) (LEPIDOPTERA: GEOMETRIDAE)

  • Leader : MARIANA OLIVEIRA BREDA
  • MEMBRES DE LA BANQUE :
  • ALICE MARIA NASCIMENTO DE ARAUJO
  • ELIO CESAR GUZZO
  • HENRIQUE FONSECA GOULART
  • JAKELINE MARIA DOS SANTOS
  • MARIANA OLIVEIRA BREDA
  • VANDERSON BARBOSA BERNARDO
  • Data: 20 déc. 2023


  • Afficher le Résumé
  • Among the pest insects associated with eucalyptus culture, the brown eucalyptus caterpillar, Thyrinteina arnobia (Stoll, 1782) (Lepidoptera: Geometridae), derived from native myrtaceous trees, such as guava (Psidium guajava L.), is considered the main defoliating species of the genus Eucalyptus in the country. In this sense, different methods that fit the premises of the Integrated Management of Forest Pests (IPM- Forest) have been the focus of research, including the use of biological control, through the conservation or multiplication and release of natural enemies. The gregarious endoparasitoid Palmistichus elaeisis La Salle (Hymenoptera: Eulophidae) is considered a promising agent in the control of defoliating lepidoptera, having generalist habits. For complete elucidation of the interactions that permeate the use of parasitoids in biological control programs, the understanding of the host search process is fundamental. In view of the above, the objective of this study was to carry out survey studies of natural enemies of spontaneous occurrence of T. arnobia in forest stands of Eucalyptus spp., in addition to the evaluation of the chemical ecology in the use of the endoparasitoid P. elaeisis, aiming at directing the bases for the planning and implementation of the conservative and augmentative biological control of T. arnobia in the state of Alagoas. It was possible to register the spontaneous occurrence of the natural enemies, Winthemia sp. (Diptera: Tachinidae), Charops sp. (Hymenoptera: Ichneumonidae, Campopleiginae), Glyptapanteles sp. (Hymenoptera: Braconidae) and the predator Podisus sp. (Hemiptera: Pentatomidae) associated with T. arnobia in Eucalyptus sp. in the state of Alagoas. Compounds found in pupae of T. arnobia, indicated as 4-Hexen-1-ol, acetate; 2,3,5,6-Tetramethylpyrazine; 2-Nonanone and 1-Undecene present bioactivity in processes of attraction of the parasitoid P. elaisis. The results obtained contribute to the consolidation of the Integrated Management of Forest Pests (IPM- Forest).

2022
Thèses
1
  • LARISSE ARAUJO DE ABREU
  • OCCURRENCE OF Colletotrichum spp. ASSOCIATED WITH mistletoe IN THE STATE OF ALAGOAS
  • Leader : GAUS SILVESTRE DE ANDRADE LIMA
  • MEMBRES DE LA BANQUE :
  • GAUS SILVESTRE DE ANDRADE LIMA
  • JAQUELINE FIGUEREDO DE OLIVEIRA COSTA
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • Data: 10 mars 2022


  • Afficher le Résumé
  • Parasitic plants complete their life cycle associated with the host, and may be hemiparasites or holoparasites. Many parasitic plants belong to the Loranthaceae family, order Santalales, and are popularly known as mistletoes, which use a specialized organ, known as a haustorium, to remove water and mineral salts from their hosts. The mistletoes can be affected by diseases such as anthracnose, caused by species of the genus Colletotrichum. The study of Colletotrichum species that infect parasitic plants can be used as alternatives for the biological control of these plants. However, few studies have been conducted focusing on this theme. Given the above, the objective of the present study was to identify the species of the genus Colletotrichum in parasitic plants in the municipalities of Maceió and Rio Largo, Alagoas. Ten isolates of Colletotrichum were obtained from parasitic plants of the genera Psittacanthus and Struthanthus. For cultural characterization, the colony color and the mycelial growth of the isolates were evaluated at 25ºC in four different culture media, namely BDA, SNA, cornmeal and oat. For morphological characterization, the length and width of conidia were measured. Pathogenicity tests were performed on leaves detached from the respective hosts, and all isolates proved to be pathogenic.

2
  • CESAR GONCALVES DOS SANTOS
  • INTERACTIONS BETWEEN Rhynchophorus palmarum LINNAEUS, 1758 (COLEOPTERA: CURCULIONIDAE) AND Billaea rhynchophorae BLANCHARD, 1937 (DIPTERA: TACHINIDAE)

  • Leader : ANTONIO EUZEBIO GOULART SANTANA
  • MEMBRES DE LA BANQUE :
  • ANTONIO EUZEBIO GOULART SANTANA
  • ELIO CESAR GUZZO
  • NIVIA DA SILVA DIAS PINI
  • Data: 28 mars 2022


  • Afficher le Résumé
  • Rhynchophorus palmarum Linnaeus, 1758 (Coleoptera: Curculionidae) is the main pest of arecaceae in the Neotropical region, occurring in several Brazilian states, attacking, in addition to palm trees, several cultures of economic importance. In coconut and oil palm, it causes direct and indirect damage, leading to reduced productivity. Considering that there is no chemical product registered to combat this pest, the most efficient control methods are cultural, behavioral and biological.  Parasitoid flies of the genus Billaea Robineau-Desvoidy, 1830 (Diptera: Tachinidae) have been used to control pests of large crops in Latin America, and in Brazil the natural parasitism of Billaea spp. in oil palm plantations infested with R. palmarum, but there are no studies on the chemical ecology of these natural enemies. Thus, the present work aimed to identify Volatile Organic Compounds (VOCs) involved in the pest-parasitoid interaction, as well as the cuticular compounds of B. rhynchophorae Blanchard, 1937. The pest and the parasitoid were collected in oil palm plantations in the state of Bahia, transported to the Natural Resources Research Laboratory (LPqRN) and packed in BOD. The collection of volatiles of R. palmarum larvae and oil palm stem were obtained through the dynamic headspace technique, cuticular extractions of different parts of B. rhynchophorae were also performed using solvent. The headspace and cuticular extracts were analyzed and the compounds identified by means of Gas Chromatography coupled to the Flame Ionization Detector (GC-FID) and Gas Chromatography coupled to Mass Spectrometry (GC-MS). Chemical analyzes revealed the presence of 17 compounds in the aeration extracts and seven (7) in the cuticular extracts. In the electroantenography bioassays, three compounds present in the headspace were active for B. rhynchophorae females. The compounds identified in the cuticular extracts are similar between the analyzed parts and belong to the hydrocarbon class.

3
  • ALEX BÉU SANTOS
  • Activity and determination of parameters for the use of the pheromone of Opsiphanes invirae (Lepidoptera:Nymphalidae) as a control strategy in field
  • Leader : HENRIQUE FONSECA GOULART
  • MEMBRES DE LA BANQUE :
  • ANTONIO EUZEBIO GOULART SANTANA
  • GAUS SILVESTRE DE ANDRADE LIMA
  • GILSON SANCHES CHIA
  • HENRIQUE FONSECA GOULART
  • JOSEANI CASTRO DA SILVA
  • ROBERTO RAMOS SOBRINHO
  • Data: 29 août 2022


  • Afficher le Résumé
  • Opsiphanes invirae (Hübner, 1818) (Lepidoptera: Nymphalidae) is a pest that occurs
    throughout Brazil and, in its young phase, causes defoliation in maples, which causes a
    reduction in productivity. Pará, where the largest oil palm plantations (Elaeis guineensis
    Jacquin, Arecaceae) in the country are concentrated, is one of the states most affected
    by the pest attack. Due to the damage caused by the conventional practice of pest
    control, it is necessary to seek less harmful forms of control. Thus, the objective of this
    work was to prove the activity of the O. invirae pheromone and determine parameters
    for its use in the control of the pest, in the field, from the use of traps for monitoring and
    population control. It was carried out at the Natural Resources Research Laboratory
    (LPqRN), which belongs to the Campus of Engineering and Agricultural Sciences
    (CECA) of the Federal University of Alagoas (UFAL), in the city of Maceió, state of
    Alagoas, and on the farms of the company Agropalma, area of commercial oil palm
    cultivation, located in the municipality of Thailand, Pará. The study of the volatiles
    present in the sugarcane molasses obtained by SPME/GC/MS was carried out in the
    laboratory, which resulted in 21 compounds present in the sugarcane molasses, of which
    2 were confirmed by the standards commercials. The confirmed compounds, acetoin
    and Z9:14Ac and the one suggested in chromatograms 3-methyl-1-butanol, are efficient
    attractants for insects of the lepidopteran order. For the field experiment, traps made
    with plastic bags were used, containing, inside, sugarcane molasses as a food attractant
    and the pheromone to monitor the capture of insects. Two containers with the attractants
    were used, in one, a rubber set type, the pheromone was placed, and in the other, a pet-
    type bottle with a capacity of 500 mL with small perforations in its upper part, holding
    about 100 ml of the food attractant, which will have in its composition 50% sugarcane
    molasses and 50% water. The two containers were fixed inside the bag by 15 and 25 cm
    wires. These traps were 80 cm high by 55 cm long and were fixed to the plants, with an
    opening of eight centimeters, for the entry of insects. Field tests allow us to conclude
    that treatment one, containing the male pheromone mixture of Opsiphanes invirae, (E)-
    β-farnesene); (E)-nerolidol and (Z)-7-heptadecene, showed greater efficiency in
    capturing both male and female insects, which proves that the use of this formulation in
    combination with sugarcane molasses can significantly contribute to reduce the
    population density of the pest in oil palm plantations.

4
  • ALEXSANDRO GONÇALVES PACHECO
  • ATTRACTIVE RESPONSE OF DIFFERENT SYNTHETIC PHEROMONES AND KAYROMONES ON Rhynchophorus palmarum L. (1764) (COLEOPTERA: CURCULIONIDAE) AND Metamasius Hemipeterus L. (1764) (COLEOPTERA: CURCULIONIDAE)

  • Leader : HENRIQUE FONSECA GOULART
  • MEMBRES DE LA BANQUE :
  • ALDOMARIO SANTO NEGRISOLI JUNIOR
  • ANTONIO EUZEBIO GOULART SANTANA
  • HENRIQUE FONSECA GOULART
  • JAKELINE MARIA DOS SANTOS
  • ROSEANE CRISTINA PREDES TRINDADE
  • Data: 29 août 2022


  • Afficher le Résumé
  • Numerous species of palm trees are plants of great economic value, being among the main sources of non-timber forest resources, with several uses and generating employment and income for several farmers. The incidence of pests in palm trees is a limiting factor for exploration, accounting significantly for the general depletion of the crop and also for the reduction in productivity.This situation is aggravated by the occurrence of pests such as Rhynchophorus palmarum L. (Coleoptera: Curculionidae) and Metamasius hemipterus L. (1764) (Coleoptera: Curculionidae), the damage to the crop is caused by the larvae that make galleries in the apical meristem of the palm trees, causing the reduced tillering and opening of holes can serve as a gateway for phytopathogens, in addition they can also be vectors of diseases that can lead to plant death. Semiochemicals can alter the behavior of insects, thus allowing the development of sustainable pest control strategies. To date, there is no information in the literature or in the Ministério da Agricultura, Pecuária e Abastecimento (MAPA) on a potentially efficient insecticide for the management of R. palmarum and M. hemipterus. Currently, control with the use of traps with attractants has been the most efficient form of control to reduce the populations of these insect pests. Therefore, the objective of this work was to analyze the efficiency of synthetic pheromones and kairomones in alphatometry and electroantenography tests on the attractiveness of R.palmarum and M.hemipterus as a measure of behavioral control. The work was carried out in the Laboratório de Pesquisas em Recursos Naturais (LPqRN)from the Institute of Chemistry and Biotechnology of the Universidade Federal de Alagoas – Campus A.C. Simões. It was concluded that males and females of R.palmarum present electroantenographic responses to the compound acetoin, being more attractive in males than females. Males and females of R.palmarum and M. hemipterus showed a better response to the Meta 1 compound, when compared to the compounds, Meta 2, Rincophorol and Coconut oil.
Thèses
1
  • DÉBORA CRISTINA DA SILVA
  • FUNGI CAUSING POST-HARVEST ROT IN BANANA AND ANTHRACNOSIS SEVERITY IN PROCESSING UNITS IN CARIRI CEARENSE

  • Leader : KAMILA CÂMARA CORREIA
  • MEMBRES DE LA BANQUE :
  • ANA PAULA OLIVEIRA DE BARROS
  • ALICE MARIA GONÇALVES SANTOS
  • GAUS SILVESTRE DE ANDRADE LIMA
  • KAMILA CÂMARA CORREIA
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • Data: 24 févr. 2022


  • Afficher le Résumé
  • Crown rot and anthracnose are important banana diseases worldwide. Currently, there is little information about the etiology of crown rot, and there is a need to accurately identify its causal agents for the development of efficient management measures. For anthracnose, several studies on the etiology and epidemiology are available. However, the behavior of this disease in the post-harvest phases of the fruit is not known. Thus, the present work aimed to identify the species of Nigrospora associated with crown rot and monitor the severity of anthracnose in bananas collected in fruit processing stages in Cariri, Ceará. To identify the species of Nigrosporas, isolates obtained from the crown of bananas with symptoms of rot, and with morphological characteristics similar to the genus, were submitted to the process of DNA extraction and sequencing of the ITS region and the TUB2 and EF1-α genes. After identification, the pathogenicity and aggressiveness of the isolates were evaluated. Based on morphological and genetic analysis, the isolates obtained belong to the species N. hainensis and N. lacticolonia. All isolates induced necrotic lesions in the inoculated fruits. This is the first report of a genetically characterized Nigrospora species associated with postharvest rot in bananas. To assess the severity of anthracnose, bananas were collected in three stages of post-harvest processing in two periods of the year. The bananas were placed in a humid chamber until the appearance of symptoms and the severity of the disease was evaluated with the aid of a diagrammatic scale. There was no significant difference between the levels of anthracnose severity between samples within the same period of the year. On the other hand, there was a significant difference in the severity levels between the periods of the year and between the post-harvest processing stages. In most situations, the severity of anthracnose was higher in the wet season. However, no pattern was found in the levels of disease severity in the different stages of processing. In Cariri Ceará, N. lacticolonia is the Nigrospora species most frequently associated with crown rot and the severity of anthracnose in the post-harvest processing stages is associated with the routines adopted in banana production areas.

2
  • EDSON FERREIRA DE LIMA
  • Bioecological aspects of Zagreus bimaculosus (Mulsant) (Coleoptera: Coccinellidae) on scale and flour mealybugs.
  • Leader : ROSEANE CRISTINA PREDES TRINDADE
  • MEMBRES DE LA BANQUE :
  • ANTONIO EUZEBIO GOULART SANTANA
  • DOUGLAS RAFAEL E SILVA BARBOSA
  • EDMILSON SANTOS SILVA
  • Glaucilane dos Santos Cruz
  • MAURICIO SILVA DE LIMA
  • Data: 25 févr. 2022


  • Afficher le Résumé
  • Coccinellids are an important group of insects that are known as ladybugs. Its varied size, of different colors and stains on its elytra, whether traces or macules. The body has a more or less oval shape with a convex dorsal surface and a flat ventral surface, as they have the ability to feed on a wide range of herbivorous insects such as aphids, mealybugs, psyllids, thrips and lepidopteran larvae, they are excellent biological control agents. of various pests in various agroecosystems. Among the various pests that affect crops, mealybugs belonging to the Diaspididae and Pseudococcidae families, popularly known as scale and mealybugs, have stood out for the fact that they attack a wide range of cultivated plants, which has brought numerous losses to producers. Considering the potential that coccinellids have as biological controllers of this group of insects, the main objective of this work was to compare the biology and predatory capacity of Z. bimaculosus, feeding on Diaspis echinocacti and Planococcus citri, as well as to test the predator's viability in the fourth-instar and adult larval stages on mealybugs F. dasylirii (Cockerell) (Hemiptera: Pseudococcidae) and P. citri (Risso) (Hemiptera: Pseudococcidae). For this, the comparative biology of Z. bimaculosus feeding on two different preys, D. echinocacti scale mealybug and P. citri mealy mealybug, was performed. The results showed differences in some biological variables, such as the development time being shorter for those fed with D. echinocacti. In the second experiment, the predation rate and the functional response of larvae and adults of Z. bimaculosus were analyzed against two phases (nymphs and adults) of mealybugs, F. dasylirii and P. citri, in both experiments, both larvae and Z. bimaculosus adults showed a type II functional response, about handling time, this time was shorter for adults when compared to larvae. The results of this work showed that Z. bimaculosus presents itself as a potential controller of Diaspididae and Pseudococcidae mealybugs, since it can complete its cycle and reproduce with these types of prey, indicating that it is an ideal food for this predator. The functional responses indicate that Z. bimaculosus is an efficient predator for the control of these mealybug species.

3
  • RENATA PEREIRA DE LIMA
  • LIFE STORY OF Tetranychus bastosi TUTTLE, BAKER & SALES, 1977 (ACARI: TETRANYCHIDAE) IN ALAGOAS STATE

  • Leader : MARIANA OLIVEIRA BREDA
  • MEMBRES DE LA BANQUE :
  • MARIANA OLIVEIRA BREDA
  • ELIO CESAR GUZZO
  • MAURICIO SILVA DE LIMA
  • ALICE MARIA NASCIMENTO DE ARAUJO
  • JOSE VARGAS DE OLIVEIRA
  • Data: 25 févr. 2022


  • Afficher le Résumé
  • Tetranychus bastosi (Tuttle, Baker & Sales), 1977 (Acari: Tetranychidae) was described in the state of Ceará and until now the occurrence of this species is restricted to Brazil. It is a phytophagous mite registered in more than 36 plant hosts, including species of agricultural importance. Thus, the objectives of this study were to report the first record of the occurrence of T. bastosi in the state of Alagoas, in addition to evaluating the life history of T. bastosi in different hosts. In Alagoas, T. bastosi was reported for the first time, infesting plants of Turnera subulata L. and Ipomoea batatas L., causing significant damage. The population growth of T. bastosi showed positive values of 0.35 ± 0.02 and 0.21 ± 0.09 for I. batatas and T. subulata, respectively, with significant differences between them. To the study of the life history of T. bastosi, the parameters duration and survival of immature stages, longevity and fecundity were evaluated. From the results, fertility life tables were prepared for three plant species: Morus rubra L. considered the first host for this mite in Brazil; I. batatas, first reported host in the state of Alagoas; and Psidium guajava L. a host reported for the Northeast of Brazil. Instantaneous rates of growth (ri), food preference and oviposition were also evaluated for T. bastosi. The incubation period of T. bastosi eggs was longer on M. rubra (5.9 days) followed by I. batatas (4.0 days) and P. guajava (4.0 days). The longest larval period was observed on P. guajava (5.7 days). The longest durations of the protonymph and deutonymph stages were observed in P guajava (3 days) and M. rubra (2.3 days), respectively. The longest period from egg to adult of T. bastosi was observed on I. batatas (30 days). The highest longevity of T. bastosi was observed on the hosts I. batatas and M. rubra (39.8 and 30.2 days, respectively). The mite T. bastosi showed the highest net rate (Ro) and longest mean duration of a generation (T) on M. rubra (13.0 and 21.3 days), but the highest innate capacity for population increase (rm) and finite rate of increase (λ) were observed on I. batatas (0.15 and 1.16). The host preference tests indicated that there was a preference of T. bastosi for P. guajava in all of the analyzed periods and bioassays, however, no preference was observed for oviposition among the hosts. The instantaneous rate of growth (ri) of T. bastosi showed positive values of 0.3509 ± 0.01 and 0.275 ± 0.012 for I. batatas and M. rubra, respectively, however a negative rate of -0,082± 0,02 was observed for the host P. guajava.

4
  • ANDERSON BRUNO ANACLETO DE ANDRADE
  • Chemical ecology of Arecaceae defoliating pests: Coraliomela brunnea Thumberg, (Coleoptera: Chrysomelidae) and Brassolis sophorae Linnaeus (Lepidoptera: Nymphalidae)

  • Leader : ANTONIO EUZEBIO GOULART SANTANA
  • MEMBRES DE LA BANQUE :
  • ANTONIO EUZEBIO GOULART SANTANA
  • ELIO CESAR GUZZO
  • ROSEANE CRISTINA PREDES TRINDADE
  • MARILIA OLIVEIRA FONSECA GOULART
  • RICARDO SALES TINOCO
  • Data: 16 août 2022


  • Afficher le Résumé
  • The palm trees have economic and ornamental importance. Its cultivation is usually attacked by several pests, including defoliating insects, such as the caterpillar Brassolis sophorae and the beetle Coraliomela brunnea. The monitoring and development of control methods are essential in the search for integrated management of these insect pests. The present work aims to investigate the semiochemicals associated with B. sophorae and C. brunnea to control and monitor these pests of Arecaceae. Through the description of morphological and behavioral characteristics, analysis of volatiles emitted by coconut palm leaves, identification of compounds associated with insect body parts with the possible pheromone function and testing of these compounds through bioassays and verification of attractive activity. In the morphological characteristics, we observed that in the antennae of C. brunnea the sensory structures are trichoid sensilla, with no difference between the sexes. In addition, the females of C. brunnea have a longer and wider body, and the fifth urosternite is longer than males, allowing sex differentiation in adults. In B. sophorae antennae, the sensilla are of three types, trichoid, chaetic and coeloconic, with no difference between sexes. In the hindwings of males of B. sophorae, there is the presence of androconia. This morphological structure serves as sexual dimorphism and may be associated with the release of the male-specific coumaran compound, which has bioactivity for females. The volatile compounds of green leaves emitted by coconut leaflets are four compounds, being n-hexanal and (Z)-3-Hexen-1-ol active for males of C. brunnea. The compounds present in parts of the body of C. brunnea are similar in quantity between sexes, with long-chain alkanes as the majority. The parts extracts showed two active compounds for males and females, and the male leg extract was attractive for C. brunnea females.

5
  • THIAGO ALVES PIMENTA
  • EFFECT OF CLOVE ESSENTIAL OIL AND ITS MAJORITY COMPOUND ON THE BEHAVIOR, BIOLOGY AND NUTRITION OF Sitophilus zeamais L. AND Callosobruchus maculatus
  • Leader : ROSEANE CRISTINA PREDES TRINDADE
  • MEMBRES DE LA BANQUE :
  • JAKELINE MARIA DOS SANTOS
  • MARIANA OLIVEIRA BREDA
  • MAURICIO SILVA DE LIMA
  • ROSEANE CRISTINA PREDES TRINDADE
  • THYAGO FERNANDO LISBOA RIBEIRO
  • Data: 17 août 2022


  • Afficher le Résumé
  • The culture of corn and beans stand out as the most produced and exported grains in Brazil, playing both social and economic importance, present in human and animal food due to its rich nutritional value. With the population increase, there was an increase in demand, highlighting the importance of grain storage, measures are necessary to minimize losses during this period, due to pests and diseases that can reduce their quality and commercial value. Thus, the present study aimed to evaluate the bioactivity of clove essential oil and its mode of action in the management of stored grain pests. The research was developed in the Laboratory of Alternative Pest Control-LECAP, in the Laboratory of Physiology of Insects-UFRPE and in the Laboratory of Research in Chemical Ecology -UFAL, Campus Maceió/AL. By means of chromatography, eugenol was identified as the major compound of clove oil, both the essential oil and its major compound showed effect by contact, fumigation and repellency on Sitophilus zeamais and Callosobruchus maculatus at different lethal concentrations. In addition to the major compound at different lethal concentrations, they promoted changes in the nutritional parameters of S. zeamais.

     

6
  • GEORGIA DE SOUZA PEIXINHO
  • CHARACTERIZATION OF Colletotrichum spp. ASSOCIATED WITH ANTHRACNOSIS IN TROPICAL FLOWERS IN NORTHEAST BRAZIL AND USE OF ESSENTIAL OILS IN DISEASE CONTROL

  • Leader : EDNA PEIXOTO DA ROCHA AMORIM
  • MEMBRES DE LA BANQUE :
  • EDNA PEIXOTO DA ROCHA AMORIM
  • JAQUELINE FIGUEREDO DE OLIVEIRA COSTA
  • JULIANA PAIVA CARNAUBA
  • MARIA DE FATIMA SILVA MUNIZ
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • Data: 23 août 2022


  • Afficher le Résumé
  • Anthracnose is the main disease caused by the species of this genus and occurs on a wide range of hosts. In species of the tropical flowers the disease causes serious problems in productivity, mainly in tropical and subtropical regions. Although more species of the genus have been reported causing anthracnose in tropical flowers in different parts of the world, little is known about the etiology of the disease in Brazil. Therefore, the objective of this study wereto identify species of the genus Colletotrichum through molecular and morphocultural characterization associated with different species of tropical flowers, to evaluate the cross-pathogenicity of the different species of Colletotrichum identified and to test different essential oils to control the disease. For molecular characterization, the genomic DNA of the isolates were used by the CTAB 2% protocol with modifications. The partial sequence of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was amplified using the GDF/GDR primers, for a preliminary identification of the isolates and evaluation of the haplotypic diversity of the species. Subsequently, representatives of each species, based on haplotypes, were amplified with another gene, β-tubulin (TUB2), and the ITS-rDNA region. The cultural characterization consisted of obtaining the mycelial growth of the colonies, through daily measurement in two diametrically opposite directions and observation of the color of the colonies evaluated after seven days. Morphological characterization was based on the shape and size of 50 conidia and appressoria. Length and width measurements were obtained through images captured by a digital camera attached to an optical microscope with a 400x magnification, using Cellsenses Standard software. The mycelial growth of Colletotrichum spp. in Petri dishes containing PDA medium supplemented with the following chemicals: mancozeb (240g); methyl thiophanate (45 g.L-1) and essential oils: lemongrass, citronella and mint essential oil (0.75; 1.5; 2.25 and 3%) and ADE for all controls. The selected in vitro treatments were used in the in vivo experiment, applied 72 and 48 hours before inoculation of the pathogen (resistance inducer) and 72 and 48 hours after inoculation (dressing), for the first experiment. In the second experiment, citronella and mint oils (0.25; 0.5; 0.75 and 1%) and methyl thiophanate (45 gL-1) were used, separately, 72 and 48 hours before inoculation (inducing resistance) and 72 and 48 hours after inoculation (dressing). The inflorescences were submitted to inoculation with the pathogen, through the spraying method (106 con./mL). After 4 days, the disease index and severity and incidence were determined. The data were submitted to analysis of variance (ANOVA), and when there were significant differences, the averages of the treatments were compared by the Scott-Knott Test at a 5% probability level, using the SISVAR program. The compounds present in the essential oils were determined. It was possible to identify five species within the C. gloesporioides and C. orchidearum complexes. The essential oils of citronella, lemongrass and mint reduced the severity of the disease.

7
  • LÍVIA FRANCYNE GOMES CHAVES
  • Begomovirus species infecting Cnidoscolus urens and study of Cnidoscolus mosaic leaf deformation virus transmission by seed
  • Leader : SARAH JACQUELINE CAVALCANTI DA SILVA
  • MEMBRES DE LA BANQUE :
  • GAUS SILVESTRE DE ANDRADE LIMA
  • JOÃO GOMES DA COSTA
  • MARIA DE FATIMA SILVA MUNIZ
  • ROBERTO RAMOS SOBRINHO
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • Data: 26 août 2022


  • Afficher le Résumé
  • Species of the genus Begomovirus (family Geminiviridae) cause damage to economically important crops around the world. However, wild/non-cultured hosts play a relevant epidemiological role, acting as a viral reservoir. These viruses are transmitted by a complex of cryptic species of the whitefly Bemisia tabaci, but, recently, transmissibility via seeds has been observed. Interestingly, Cnidoscolus urens plants are observed in the field with typical symptoms of begomovirus infection even in the apparent absence of the vector. In this context, the aim of the present study was to molecularly characterize new begomoviruses infecting C. urens, as well as to study the possible transmission of viruses by seeds. Leaf samples showing symptoms indicative of begomovirus infection were collected in the states of Pernambuco and Rio Grande do Norte, between 2019 and 2020. Total DNA was extracted from cansanção plants and later used as a template for rolling circle amplification (RCA), followed by cloning and sequencing by primer walking. In Paripueira, Alagoas, leaves, flowers and seeds of symptomatic plants were collected, as well as seedlings from infected seeds, and tested for Cnidoscolus mosaic leaf deformation virus (CnMLDV), using species-specific primers. A total of six clones of the DNA-A genomic component and two DNA-B clones were obtained. The DNA-A of the isolate from Paulista - PE shared the highest percentage of nucleotide identity (79.9%) with the Tomato common mosaic virus (ToCmMV; accession number KC706579), while the DNA-A from the isolate from Cabo de Santo Agostinho – PE obtained the highest percentage of nucleotide identity (88.9%) with CnMLDV (NC038982). The DNA-A component of the isolate from Goianinha - RN also shared the highest percentage of nucleotide identity (82.8%) with CnMLDV (NC038982). Based on the classification criteria for the genus Begomovirus, we report three new members infecting C. urens, and the names Begomovirus paulitiensis, Begomovirus caboniensis and Begomovirus goianiensis, respectively, are proposed for these viruses, adopting the binomial system. The results of detection of CnMLDV in plant parts revealed that 62% of the sepals, 79% of the androceum and 83% of the flowers, petals and gynoecium were infected. More than 90% of the seeds collected from C. urens infected with CnMLDV were positive. CnMLDV was also identified from integument, endosperm and embryo. However, no evidence of transmission of CnMLDV from seeds to seedlings was observed. Thus, suggesting that transmissibility via seeds is not a general property of the CnMLDV begomovirus.

8
  • ANA CAROLINE DE MELO BASTOS MORAIS
  • INDUCTION OF RESISTANCE TO Colletotrichum truncatum IN Phaseolus lunatus BY NON-PATHOGENIC ISOLATE OF Fusarium spp. and Rhizobium spp.

  • Leader : GILDEMBERG AMORIM LEAL JUNIOR
  • MEMBRES DE LA BANQUE :
  • EDNA PEIXOTO DA ROCHA AMORIM
  • GILDEMBERG AMORIM LEAL JUNIOR
  • MARIA DE FATIMA SILVA MUNIZ
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • TAMARA RODRIGUES DOS SANTOS
  • Data: 31 août 2022


  • Afficher le Résumé
  • The resistance induction process is the previous activation of the plant's defense mechanisms by biotic or abiotic agents. Induced defense processes can be of two types: acquired systemic resistance (SAR) or induced systemic resistance (ISR). ISR is activated by a microorganism from the rhizosphere and isolates of Fusarium oxysporum and Rhizobium etli have already been characterized as resistance inducers in several cultures. This work aimed to verify the effect of Fusarium spp. and Rhizobium spp. obtained from Phaseolus lunatus in the activation of the defense response against the causal agent of anthracnose, Colletotrichum truncatum. The experiment began with obtaining isolates of Fusarium spp. non-pathogenic and Rhizobium spp., after that the morphological characterization was carried out, the evaluation of the direct antibiosis of Fusarium spp. and Rhizobium spp. in relation to Colletotrichum truncatum. The selection of material for analysis of resistance enzymes was made from production experiments (weight of fresh and dry matter of shoot and root and evaluation of resistance), then the analysis of peroxidase and phenylalanine ammonia-lyase enzymes was performed. The results were submitted to analysis of variance at 5% probability and the Scott-Knott test (p = 5%). Isolates of Fusarium spp. FF 11.1 and FF 3 and the isolate B. Nelita from Rhizobium spp. were able to reduce disease severity and induce resistance.

2021
Thèses
1
  • ALESKA BATISTA DA SILVA
  • PLASTER AS AN ESSENTIAL OIL DISPERSING AGENT IN STORED GRAIN PEST CONTROL
  • Leader : ROSEANE CRISTINA PREDES TRINDADE
  • MEMBRES DE LA BANQUE :
  • ROSEANE CRISTINA PREDES TRINDADE
  • MARIANA OLIVEIRA BREDA
  • SOLANGE MARIA DE FRANÇA
  • Data: 30 mars 2021


  • Afficher le Résumé
  • The stored grain pests, depending on the population level, can cause total deterioration of the products. The control of these pest insects is usually done through purge or fumigation, but studies using secondary compounds obtained from plants, mainly with essential oils and vegetable powders, have expanded, due to the proven efficiency in research of these against stored grain pests. However, its use in practice is still a challenge for research, as it is volatile, it disperses very quickly, losing its effect with great ease. Thus, the objective of this work was to develop a natural dispersant based on plaster to facilitate the application in practice of the essential oil of Mentha piperita in the control of adults of Sitophilus zeamais Mots. 1763 (Coleoptera: Curculionidae). Each dispersant was prepared with a mixture of 8.5 g of plaster in 5.5 ml of water, being molded in aluminum forms of 0.3 x 0.2 x 0.1 cm. The evaluations of the efficiency of the plaster dispersant were made by the following tests: of fumigation in glass containers of 1.3 L of capacity with 20 adults of S. sitophilus, up to 15 days of age, not sexed, being evaluated with 48h at percentage of mortality; and repellency, in arenas made up of three linearly interconnected glass containers, with 20 g of corn kernels being added to the end containers, constituting the container without treatment (control) on one side and on the other side, the treatments and, in the central container, 16 non-sexed adults of S. zeamais up to 15 days of age were released. After 48 h, the insects contained in each container were quantified, for the evaluation of repellency using the formula: I.R. = 2G / (G + P). The treatments and, in the central container, 16 non-sexed adults of S. zeamais up to 15 days old were released. After 48 h, the insects contained in each container were quantified, for the evaluation of repellency using the formula: I.R. = 2G / (G + P). The persistence of the fumigant and repellent effects of essential oil and plaster dispersant was also evaluated. In the fumigation test, with essential oil and plaster dispersant, the LC50 was estimated at 23.78; 34.32 and CL99 of 54.27; 79.28 μL 40g of grains, respectively. For the contact test with essential oil only, the sublethal and lethal concentrations were (CL50) 25.84 and (CL99) 48.40 μL 40g of grains, respectively. For the contact test with essential oil only, the sublethal and lethal concentrations were (CL50) 25.84 and (CL99) 48.40 μL 40g of grains, respectively. In the repellency test, only the concentration of 26 μL was repellent for both the essential oil and the dispersant. In the assessment of persistence, the repellent and fumigant effect of essential oil and plaster dispersant were 15 and 24 days, and 21 and 30 days, respectively. Thus, it is concluded that the plaster dispersant proved to be quite promising, as it has a fumigant and repellent effect against S. zeamais, and contributed to a greater persistence when compared to the application of oil without the dispersant. This result is innovative in the area of alternative control with stored grain pests.

2
  • ROMÁRIO GUIMARÂES VERÇOSA DE ARAÚJO
  • INDUCTION OF RESISTANCE TO Colletotrichum truncatum IN Phaseolus lunatus BY NITROGEN-FIXING BACTERIA

  • Leader : GILDEMBERG AMORIM LEAL JUNIOR
  • MEMBRES DE LA BANQUE :
  • GILDEMBERG AMORIM LEAL JUNIOR
  • MARIA DE FATIMA SILVA MUNIZ
  • TANIA MARTA CARVALHO DOS SANTOS
  • Data: 6 juil. 2021


  • Afficher le Résumé
  • Plant defense mechanisms are activated when challenged with the causative agent of the disease. Induced defense processes can be of two types: acquired systemic resistance (SAR) and induced systemic resistance (ISR). SAR is activated by biotic and abiotic factors and ISR is activated by microorganisms present in the rhizosphere. Nitrogen-fixing bacteria are symbionts of the roots that supply nitrogen to legumes. The interaction involves the suppression of the plant's defense system, but there are reports of effects associated with the induction of resistance to some pathogens. Thus, the objective was to evaluate the contribution of symbiosis in the resistance of P. lunatus to anthracnose. Species of nitrogen-fixing bacteria were isolated from nodules obtained from spontaneous infection of 19 P. lunatus genotypes. The isolated bacteria were morphologically characterized. Two rhizobial strains were selected, one with high nodule potential (CECA R19) and one with low nodule capacity (CECA R01). The bacterial isolates were inoculated into 4 broad bean genotypes with the following characteristics: F1CGG (high nodulation rate and resistant to anthracnose); RTKC (high nodulation rate and susceptible to anthracnose); PCGG (low nodulation rate and resistant to anthracnose); FFG (low nodulation rate and susceptible to anthracnose) and compared with the control treatment and the treatment where mineral nitrogen was supplied (corresponding to 50 kg ha-1 of N). It was found that plants inoculated with isolates CECA R01, CECA R19 and plants fertilized with mineral nitrogen showed less severity of anthracnose symptoms at 3 and 5 days after inoculation, in addition to presenting higher biomass and dry matter of the aerial part when compared to the control treatment. At the end of the evaluation, the plants fertilized with mineral nitrogen and those inoculated with the strains CECA R01 CECA R19 were classified as moderately resistant and the plants of the control treatment as moderately susceptible.

3
  • DIEGO JORGE DA SILVA
  • Bioecological aspects of Billaea rhynchophorae (Blanchard, 1937) (Diptera: Tachinidae), aiming at establishing a rearing method in laboratory

  • Leader : ELIO CESAR GUZZO
  • MEMBRES DE LA BANQUE :
  • ELIO CESAR GUZZO
  • MAURICIO SILVA DE LIMA
  • ALDOMARIO SANTO NEGRISOLI JUNIOR
  • Data: 26 juil. 2021


  • Afficher le Résumé
  • Coconut (Cocos nucifera L.) is a crop of great economic importance due to the consumption of fruits and derivatives, but it is currently being attacked by several pests, mainly by Rhynchophorus palmarum L., 1764 (Coleoptera: Curculionidae), which causes direct and indirect damage. One form of control is the use of traps with the species' pheromone, contributing to the decline of the population in the field. New studies with parasitoid insects of the genus Billaea (Robineau-Desvoidy, 1830) (Diptera: Tachinidae) may contribute to the control of R. palmarum and other beetles. The objective of this work was to evaluate different food sources on the survival and parasitism of B. rhynchophorae in R. palmarum larvae. The collections of larvae and pupae of the parasitoid and flowering plants were carried out in oil palm plantations in the municipalities of Jaguaripe and Taperoá, both located in the state of Bahia, and sent to the laboratory. To assess the longevity of B. rhynchophorae, two trials were carried out: in the first, I) sugarcane stalks; II) chamomile Matricaria chamomilla L. (Asteraceae) flowers; III) palm Adonidia merrillii Becc. (Arecaceae) flowers and in the second, I) 20% honey; II) 33% sucrose solution; III) 3g of bee pollen. As for the natural parasitism, flies aged between 24 and 48 hours were used, to which larvae of R. palmarum were offered, and the viability of planidia was also evaluated through the following ages of the flies 8, 10, 12 and 14 days old. The experiment of the influence of food sources was carried out in a completely randomized design. In all experiments, the means obtained in the different treatments were submitted to analysis of variance and, when necessary, compared to each other by Tukey's test at 5% significance, using the statistical program SASM-Agri. All plants collected in oil palms were identified, among them Ageratum conyzoides and A. uliginosa are two species belonging to the Asteraceae family, the same family as chamomile, M. chamomilla, the species used in this work as a food source for the parasitoids. However, in the first trial it was found that sugarcane differed statistically from palm flower and chamomile flower. In the second experiment, it could be verified that pollen and 20% honey differed from 33% sucrose. In the evaluation of natural parasitism, none of the treatments (palm flower, chamomile flower and sugarcane) provided sexual maturation of the flies, and planidia viability in the different days of age of the parasitoid. Therefore, the food sources sugarcane, 20% honey and bee pollen favored a greater mean longevity of B. rhynchophorae, however, sugarcane, palm flower and chamomile flower did not contribute to sexual maturation, as well as the viability of planidia in the different days of evaluation.

4
  • TAMARA TAIS DOS SANTOS
  • Biological aspects of the parasitoid Billaea rhynchophorae (Blanchard, 1937) (Diptera: Tachinidae)in its host Rhynchophorus palmarum (L., 1758) (Coleoptera: Curculionidae)

  • Leader : ELIO CESAR GUZZO
  • MEMBRES DE LA BANQUE :
  • ALDOMARIO SANTO NEGRISOLI JUNIOR
  • ELIO CESAR GUZZO
  • MAURICIO SILVA DE LIMA
  • Data: 27 juil. 2021


  • Afficher le Résumé
  • The species Rhynchophorus palmarum (L., 1758) (Coleoptera: Curculionidae), occurring in almost the entire tropical region of the planet, is considered the main pest of coconut (Cocos nucifera L.) and oil palm (Elaeis guineensis Jacq.). Tachinids are very important entomophagous dipterans in the parasitism of borers in America, comprising several species cataloged as parasitoids, which are used for the biological control of harmful insects. Although the first detection of parasitoid flies of the genus Billaea on R. palmarum occurred more than 20 years ago, to date, there is no detailed information about its biology. The aim of this study was to understand the biological aspects of Billaea rhynchophorae (Blanchard, 1937) (Diptera: Tachinidae), to help develop strategies that can be used to control R. palmarum and possibly other related pests. The parasitoids were collected in oil palm plantations, their naturally occurring environment. The collections were carried out in September/2019, December/2019 and November/2020 in Jaguaripe and Taperoá, in the south of the state of Bahia. The cocoons of R. palmarum with the presence of larvae and pupae of B. rhynchophorae, as well as larvae and pupae (live and dead) possibly parasitized and adults of the host were transported to the Entomology Laboratory of Embrapa Tabuleiros Costeiros, Unidade de Execução de Pesquisa e Desenvolvimento de Rio Largo, AL, where the rest of the work was conducted. According to the emergence of the parasitoids, the flies were distributed in wooden cages and fed with the nectarous plant chamomile (Matricaria chamomilla L). The following parameters were evaluated: parasitism index, pupal viability, pupal duration, weight, length and diameter of puparia, sex ratio, longevity of adult insects, number and length of planidia. The means were submitted to analysis of variance and compared by Tukey test at 5% probability, using the SASM–Agri program. The percentage of parasitism of B. rhynchophorae in September/2019 differed statistically from the other collections carried out, with parasitism peaks of more than 60% in December/2019 and November/2020. In laboratory, the mean viability of pupae brought from the field was 41.83%, with mean pupal duration of 45.54 ± 0.27. The sex ratio of the parasitoid flies was 0.59, with a mean longevity of 8.61 ± 0.18 days, with no significant difference between the longevity of males and females. With this study, it was possible to know some of the biological aspects of the parasitoid in the laboratory, serving as a subsidy for carrying out studies aimed at its conservation or introduction into agricultural systems, as a biological control strategy for R. palmarum.

5
  • ALVERLAN DA SILVA ARAÚJO
  • Host status of weed species to Pratylenchus coffeae

  • Leader : MARIA DE FATIMA SILVA MUNIZ
  • MEMBRES DE LA BANQUE :
  • MARIA DE FATIMA SILVA MUNIZ
  • EDNA PEIXOTO DA ROCHA AMORIM
  • MARCELO DE MENEZES CRUZ
  • JAQUELINE FIGUEREDO DE OLIVEIRA COSTA
  • Data: 27 juil. 2021


  • Afficher le Résumé
  • Among the phytosanitary problems that affect the yam (Dioscorea sp.) crop in Brazil, dry rot disease stands out as the most important, affecting commercial rhizophores and seeds, having as causal agents the nematodes Scutellonema bradys, Pratylenchus coffeae and P. brachyurus. The objective of the present work was to evaluate the reaction of weed species to P. coffeae. Two trials were carried out in a greenhouse in a completely randomized design, with 11 treatments, represented by 10 weed species and the control (Dioscorea sp.) and eight replications. Plants were inoculated with 2,000 nematode specimens. After 60 days of inoculation, nematode populations were evaluated in roots and soil. The inoculum viability could be proven in both experiments from the control reproduction factor (Dioscorea sp.) (FR=0.710 and 3.09 respectively). Ricinus communis and Macroptilium lathyroides had similar behavior, with RF< 1 in the first experiment, RF> 1 in the second. The species Chenopodium album, Amaranthus sp., Panicum maximum, Eleusine indica, Senna occidentalis, Emilia coccinea, Euphorbia hyssopifolia and Richardia brasiliensis, differed statistically from the control for the evaluated variables, showing FR<1. It is inferred that the species R. communis and M. lathyroides have great potential to act as important reservoirs and sources of inoculum for P. coffeae. There are no records in the literature of the species M. lathyroides as a host to P. coffeae

6
  • JOICE KESSIA BARBOSA DOS SANTOS
  • IDENTIFICATION OF SEMIOCHEMISTICS INVOLVED IN THE COMMUNICATION OF THE URBAN PEST Euchroma gigantea (Linnaeus 1758) (COLEOPTERA: BUPESTIDAE) IN Pachira aquatica Aublet (1775) (BOMBACACEAE)

  • Leader : HENRIQUE FONSECA GOULART
  • MEMBRES DE LA BANQUE :
  • HENRIQUE FONSECA GOULART
  • JOÃO GOMES DA COSTA
  • ROSEANE CRISTINA PREDES TRINDADE
  • Data: 27 juil. 2021


  • Afficher le Résumé
  • The great biological diversity of trees in cities contributes ecologically and
    aesthetically to make the urban environment more pleasant, performing
    a significant role in improving the living conditions of the city. At the
    However, the quality of the tree cluster can be negatively affected by
    insect attack at various stages of tree development. One
    of the main pests that attack the mungba and paineiras is the Euchroma
    gigantea L. 1758 (Coleoptera: Buprestidae), known as metallic beetle
    or South American giant buprestid, due to its size, is one of the
    arthropods that attack the bombshells. In E. gigantae attacks there is no
    information on the regulation of insects through the action of agents of
    biological control. To date, there are none in the literature or in the
    Ministry of Agriculture, Livestock and Supply (MAPA) information on
    a potentially effective insecticide for the management of E. gigantea in
    urban areas. Currently, only cultural control has been effective for
    reduction of insect pest populations, which consists of manual collection of
    adult insects and there are no reports on the action of natural control agents
    regulating the population of this pest. Given this, the work aims to
    identify volatile compounds present in males and females of Euchroma
    gigantea (Linnaeus, 1758) (Coleoptera: Buprestidae) collected from trees of
    Pachira aquatica Aubl. (1775) to obtain pheromones to be used
    in your control. The work will be developed at the Research Laboratory in
    Natural Resources (LPqRN) of the Institute of Chemistry and Biotechnology of
    Federal University of Alagoas- – A.C. Simões Campus (9°33'88”S;
    35°46'94"O) with the support of the Municipal Secretariat for the Environment of Belo
    Horizonte (SMMABH), Belo Horizonte City Hall (PBH) and the Foundation of
    Municipal and Zoobotany Parks (FPMZ) of the State of Minas Gerais.

7
  • EMANUELLE ALMEIDA DA COSTA
  • SURVEY OF THE OCCURRENCE OF MISTLETOE IN THE MATA ALAGOANA
    MICROREGION

  • Leader : GAUS SILVESTRE DE ANDRADE LIMA
  • MEMBRES DE LA BANQUE :
  • GAUS SILVESTRE DE ANDRADE LIMA
  • GILDEMBERG AMORIM LEAL JUNIOR
  • RENAN CANTALICE DE SOUZA
  • Data: 30 août 2021


  • Afficher le Résumé
  • Parasitic plants are defined as organisms that necessarily need to be associated with a host to
    ensure its life cycle. They can be classified according to their level of nutritional dependence and can
    be considered hemiparasites, holoparasites and achlorophylls. Some hemiparasites are represented
    by the botanical families Loranthaceae, Santalaceae and Viscaceae, found parasitizing trees and
    shrubs, extracting water and mineral salts from their hosts through their modified roots called
    haustoria. Given the above, this study aims to survey the occurrence of mistletoe present in the
    State of Alagoas and their respective hosts, identifying the botanical species based on morphological
    and molecular characterization. Exsiccates of Lorantaceae, Santalaceae and Viscaceae deposited in
    the MAC herbarium of the Environmental Institute of Alagoas – IMA, from collection expeditions
    carried out in the territory of Alagoas, were analyzed. And carrying out collections in the microregion
    Mata Alagoana. Total DNA was individually extracted from each plant sample, used as a template for
    PCR amplification of the ITS region sequence and sequenced. It was possible to identify three species
    of Struthanthus parasitizing different hosts, as well as the species Passovia pyrifolia and
    Psittacanthus dichroos belonging to the Loranthaceae family. For the Viscaceae family, two species
    of the genus Phoradendron were identified with a common host. Keywords: Hemiparasite.
    Morphological characterization. Molecular characterization

8
  • ELIANE DOS SANTOS
  • CHEMICAL CHARACTERIZATION OF COMPOUNDS
    INVOLVED IN THE ATTRACTIVENESS OF Bephratelloides
    pomorum (FABRICIUS, 1808) (HYMENOPTERA:
    EURYTOMYIDAE): A NEW STRATEGY FOR MIP

  • Leader : ANTONIO EUZEBIO GOULART SANTANA
  • MEMBRES DE LA BANQUE :
  • ANTONIO EUZEBIO GOULART SANTANA
  • JOÃO GOMES DA COSTA
  • ROSEANE CRISTINA PREDES TRINDADE
  • Data: 31 août 2021


  • Afficher le Résumé
  • The soursop, Annona muricata L. is a tropical annonacea, originating
    in Central America and northern South America. Due to the growth of
    soursop cultivation, problems with pests that infest different plant
    structures can cause great damage to the crop. Among these pests
    stands out the seed borer, Bephratelloides pomorum (Hymenoptera:
    Eurytomyidae), which has become one of the most important species
    in the soursop crop due to the severity of the damage it can cause.
    Being mulltivoltino and endophytic, this insect pest is difficult to
    control. In the search for an efficient control of this pest and the need
    to develop an efficient control, the interest of studying semiochemicals
    in the hope of creating an efficient strategy to monitor and control this
    pest in soursop orchards. The aim of this study was to determine
    substances involved in attractiveness among individuals of the species
    B. pomorum. Insects were collected from infested soursop fruits. These
    fruits were obtained from commercial orchards in the municipalities of
    Limoeiro de Anadia, Arapiraca and Maragogi in the state of Alagoas.
    The fruits were sent to the Laboratory of Research in Natural
    Resources (LPqRN) of the Federal University of Alagoas (UFAL) and
    kept in PVC cages, surrounded by a screen and sexed as adults. The
    collection of volatiles from the adult insects of B. pomorum was
    carried out using the methods of solvent extraction from the surface of
    the insect&#39;s body, extraction by maceration of the abdomen and
    extraction by dynamic head space of the insects. The analysis of the
    extracts by GC-DIC, GC-EAG and GC-EM led to the identification of
    the present compounds. The analysis of the extract obtained by
    dynamic headspace of females of B. pomorum led to the identification
    of 12 compounds. The electroantenography test indicated that the
    compounds 2,4-tert-butylphenol, 1-hexadecene and 2,6,10,14-
    tetramethylhexadecane were electrophysiologically active for males of 

    B. pomorum. The chromatographic analyzes indicated the presence of
    12 compounds in the dynamic head space extracts of females of B.
    pomorum, the compounds, Phytol and 2,6,10,14-
    tetramethylhexadecane were confirmed by the use of standards and
    electroantenographic studies showed that males of B. pomorum
    showed positive responses to the compound 2,6,10,14-
    tetramethylhexadecane present in the extracts.

9
  • GESSYCA THAYS DOS SANTOS SILVA
  • DYNAMICS OF FOOD SUPPLEMENTATION IN THE REPRODUCTIVE FITNESS OF Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae)
  • Leader : MARIANA OLIVEIRA BREDA
  • MEMBRES DE LA BANQUE :
  • ELIO CESAR GUZZO
  • JAKELINE MARIA DOS SANTOS
  • MARIANA OLIVEIRA BREDA
  • ROSEANE CRISTINA PREDES TRINDADE
  • WENDEL JOSÉ TELES PONTES
  • Data: 31 août 2021


  • Afficher le Résumé
  • Predators are considered the first line of defense for plants against phytophagous insects. In this group, those popularly known as ladybugs (Coleoptera: Coccinellidae) stand out, most of which are efficient predators. For this and other reasons, ladybugs are widely used in the biological control of various pests such as aphids (Aphididae), whiteflies (Aleyrodidae), scale insects (Ortheziidae, Margarodidae, Diaspididae, Coccidae, Pseudococcidae) and psyllids (Psyllidae), eggs of lepidoptera (Lepidoptera) and mites (Acari). Many species of ladybugs consume the same food in the larval and adult stages, but the food regularly consumed by adults is not always nutritionally adequate to ensure reproduction, egg maturation or larval development. Thus, the embryonic and post-embryonic development, as well as the longevity, fecundity and fertility of adults depend on the quantity and quality of food consumed by these predators. Factors such as the quantity and quality of food can affect the development, longevity and reproductive potential of insects. One of the ways to meet the nutritional requirements and improve the performance of these predators in creations developed in the laboratory can be the simultaneous offer of the prey combined with alternative foods, because foods rich in carbohydrates and proteins such as honey and pollen, respectively, can favor survival and reproductive performance of predatory ladybugs. In this way, studies on the creation, production, release and use of biological control agents are fundamental structuring steps in the generation of knowledge that produce science and technological innovations that allow the adoption and implementation of ecologically correct pest management programs that ensure levels sustainability and food security objectives in family farming production regimes or in agricultural systems with large-scale production. The fact that the predators  Cryptolaemus montrouzieri are cited as occurring naturally in areas cultivated with forage cactus and other crops with a strong tradition in northeastern Brazil contributed to the study of the potential of these predators as a control agent for scale insects and aphids. to produce technical-scientific knowledge that can be used in pest management programs for both the state of Alagoas and the Northeast region. Thus, the objective of this dissertation is to evaluate how food supplementation contributes to the improvement in the reproductive performance of adults of  C. montrouzieri, coccinellids with potential to be used as biological control agent.

10
  • JOCKELINY MAYARA CAMARA DOS SANTOS
  • SPECIES OF Colletotrichum ASSOCIATED WITH ANTHRACNOSIS

    IN ATEMOIA IN NORTHEASTERN FROM BRAZIL

  • Leader : GAUS SILVESTRE DE ANDRADE LIMA
  • MEMBRES DE LA BANQUE :
  • GAUS SILVESTRE DE ANDRADE LIMA
  • MARIOTE DOS SANTOS BRITO NETTO
  • WELLINGTON COSTA DA SILVA
  • Data: 31 août 2021


  • Afficher le Résumé
  • Therefore, the objective of the study was to identify Colletotrichum
    species associated with atemoya in the Northeast region of Brazil,
    based on multi-locus phylogeny analyzes and morphocultural
    characterization, as a way of contributing to a better understanding of
    the etiology of anthracnose disease in this culture. To obtain the
    fungal isolates of Colletotrichum, leaves and branches of atemoya
    with symptoms of anthracnose were collected in commercial
    plantations in the municipalities of Vitória da Conquista (Bahia),
    Palmeira dos Índios and Rio Largo (Alagoas), located in the Northeast
    region of Brazil. . The initial analysis of the partial sequences of the
    GAPDH gene was carried out with nineteen Colletotrichum isolates
    from leaves and branches of atemolina with typical symptoms of
    anthracnose. Seventeen isolates were grouped in the gloeosporioides
    complex and two in the Boninense complex. To confirm the species
    identified in the analysis with the GAPDH gene, eleven isolates were
    selected and submitted to phylogenetic analyzes concatenated with
    the genes (GAPDH and TUB2) and ITS region of the rDNA. The
    species C. theobromicola, C. siamense, C. fructicola and C. karstii are
    associated with atemoya anthracnose in the Northeast region of
    Brazil. The species C. fructicola and C. karstii, and C. siamense and
    C. Karstii induced the largest lesions in vitro and in vivo, respectively.

2020
Thèses
1
  • CAMILA ALEXANDRE CAVALCANTE DE ALMEIDA
  • ESTUDO FEROMONAL, PERFIL CUTICULAR E PREFERÊNCIA ALIMENTAR DA LAGARTA PARDA DO EUCALIPTO, Thyrinteina arnobia (Stoll, 1782) (LEPIDOPTERA: GEOMETRIDAE) EM DIFERENTES HOSPEDEIROS NATIVOS E EXÓTICOS

  • Leader : MARIANA OLIVEIRA BREDA
  • MEMBRES DE LA BANQUE :
  • JAKELINE MARIA DOS SANTOS
  • JOÃO GOMES DA COSTA
  • MAURICIO SILVA DE LIMA
  • Data: 14 févr. 2020


  • Afficher le Résumé
  • Com a consolidação da eucaliptocultura no Brasil, o ataque de insetos fitófagos vem adquirindo relevante importância econômica, podendo gerar perdas significativas de produção. Dentre eles, destaca-se a lagarta parda do eucalipto, Thyrinteina arnobia (Stoll, 1782) (Lepidoptera: Geometridae), considerada como a principal praga desfolhadora de Eucalyptus spp. no país. Assim, o presente estudo teve como objetivo a verificação dos processos envolvidos na interação hospedeira, a partir da caracterização do perfil cuticular e preferência alimentar de T. arnobia sobre diferentes hospedeiros nativos e exóticos, além da prospecção de substâncias feromonais de T. arnobia através do isolamento e identificação estrutural, afim de contribuir como componente básico para o desenvolvimento do Manejo Integrado de Pragas Florestais (MIP Floresta). Em testes de consumo alimentar, sem chance de escolha, observou-se maior consumo do clone VE 41 e do hospedeiro nativo goiaba (Psidium guajava L.). A avaliação do consumo alimentar, com chance de escolha, utilizando clones de E. urograndis e goiaba, não mostrou diferença significativa o que pode comprovar adaptação de T. arnobia tanto ao seu hospedeiro nativo quanto exótico. Em teste utilizando os diferentes clones de E. urograndis, o clone I 144 mostrou uma tendência de menor preferência alimentar, sendo cons umido inicialmente somente após 48h. Em perfil químico cuticular, comparando os Índices de Kovats (IK) de galhos e lagartas em goiaba e E. urograndis, foi possível observar um total de 9 compostos em comum entre galhos de goiaba e lagartas de T. arnobia e apenas 1 composto em comum quando comparada ao perfil químico dos galhos de E. urograndis. Para o estudo feromonal, os extratos cuticulares de asas e pernas de fêmeas e machos de T. arnobia não mostraram-se biologicamente ativo frente a antenas de machos. Em extratos de glândulas abdominais de fêmeas de T. arnobia foram verificados 12 compostos químicos em cromatografia gasosa acoplada a Espectometria de massas (CG-EM), sendo 9,12-octadecadienol chlorido e ácido hexadecanoico (ácido palmítico) os componentes majoritários, obtendo resposta significativa quando colocados frente a antenas de machos, via método de eletroantenografia do tipo “puff”. Os resultados obtidos são pioneiros para o desenvolvimento de estratégias de controle comportamental no Manejo Integrado de Pragas Florestais (MIP Florestas) no estado de Alagoas.

2
  • MAYARA OLIVEIRA DE LIMA
  • Detection of Sugarcane bacilliform Guadaloupe A virus using Nanopore Sequencing

  • Leader : SARAH JACQUELINE CAVALCANTI DA SILVA
  • MEMBRES DE LA BANQUE :
  • SARAH JACQUELINE CAVALCANTI DA SILVA
  • GAUS SILVESTRE DE ANDRADE LIMA
  • CICERO CARLOS DE SOUZA ALMEIDA
  • Data: 19 févr. 2020


  • Afficher le Résumé
  • Brazil is the largest producer and exporter of sugar in the world. For this purpose, the
    germplasm bank of the Genetic Improvement Program for Sugarcane (PMGCA / CECA
    / UFAL) has been essential for the development of dozens of varieties that occupy more
    60% of the country&#39;s cultivated area. In 2015, studies showed the presence of different
    species of the genus Badnavirus (family Caulimoviridae), including Sugarcane
    bacilliform AL virus (SCBALV) and probable new species, occurring in sugarcane
    genotypes from this germplasm bank, based on amplification via PCR. However, this
    technique is not able to distinguish viral sequences occurring in endogenous or episomal
    form, thus, it may not be reflecting the real diversity of badnavirus populations
    occurring in the area. Analysis using Nanopore sequencing has become a routine
    practice to detect and estimate the diversity of plant virus species. Therefore, the
    objective of the present study was to detect and characterize species of badnavirus that
    occur in sugarcane genotypes of the germplasm bank of the PMGCA / CECA / UFAL
    through sequencing by Nanopore. Total DNA was extracted from leaf samples of
    different sugarcane genotypes and used as a template for amplification via RCA. To
    detect only badnaviruses occurring in episomal form in the host plant, RCA products
    were subjected to PCR using universal primers for the genus. The positive products for
    badnavirus were sequenced using the MinION platform and performed paired and
    phylogenetic sequences. To confirm the presence of infection by the identified viral
    species, PCR amplification was performed using the species-application primers. It was
    possible to identify endogenous species of badnavirus infecting sugarcane and from
    Nanopore sequencing the presence of the Sugarcane bacilliform Guadaloupe A virus
    (SCBGAV) was identified.

3
  • JACKELINE LAURENTINO DA SILVA
  • Identificação de espécies de Colletotrichum associadas à antracnose de maracujazeiro (Passiflora edulis f. flavicarpa) em Alagoas.

  • Leader : IRAILDES PEREIRA ASSUNCAO
  • MEMBRES DE LA BANQUE :
  • FREDERICO MONTEIRO FEIJÓ
  • JAQUELINE FIGUEREDO DE OLIVEIRA COSTA
  • MARIA DE FATIMA SILVA MUNIZ
  • Data: 20 févr. 2020


  • Afficher le Résumé
  • O gênero Colletotrichum é considerado cosmopolita e tem sido um dos patógenos fúngicos mais importantes do mundo. Dentre as patologias que afetam a cultura do maracujazeiro, a antracnose é a principal doença acarretando grandes prejuízos socioeconômicos no nordeste brasileiro, no entanto, pouco se sabe sobre a etiologia da doença no Brasil. Portanto, os objetivos deste estudo foram identificar espécies do gênero Colletotrichum que infectam folhas de maracujazeiro, utilizando análises filogenéticas multi-locus associadas a características morfoculturais, bem como avaliar a virulência em gama de hospedeiros. Isolados do gênero Colletotrichum obtidos de folhas de maracujazeiro apresentado sintomas típicos da doença, foram coletados nos municípios de Coruripe, Quebrangulo e Maragogi. Os isolados patogênicos foram identificados com base nas sequências dos genes GAPDH, TUB2, CHS e região ITS-rDNA. Para caracterização cultural das espécies identificadas foram realizadas avaliações da taxa de crescimento micelial a 25 °C e aspecto das colônias. Na caracterização morfológica foram mensurados o comprimento e a largura de 50 conídios e apressórios. As análises filogenéticas multi-locus associadas a características morfoculturais de dessezete isolados revelaram seis espécies de Colletotrichum pertencentes a quatro complexos distintos: C. fructicola, C. theobromicola, C. tropicale (Complexo gloeosporioiodes), C. plurivorum (C. orchidearum), C. brevisporum (C. magnum), Colletotrichum sp. (C. boninense). Este é o primeiro relato de C. tropicale, C. fructicola e C. theobromicola no mundo e de C. brevisporum e C. plurivorum no Brasil. Todas as espécies de Colletotrichum foram patogênicas a gama de hospedeiros inoculadas (manga, maracujá, banana, mamão e goiaba), com excessão das espécies C. brevisporum, Colletotrichum sp. e C. plurivorum ao fruto de goiaba.

4
  • JACKELINE LAURENTINO DA SILVA
  • Identificação de espécies de Colletotrichum associadas à antracnose de maracujazeiro (Passiflora edulis f. flavicarpa) em Alagoas.

  • Leader : IRAILDES PEREIRA ASSUNCAO
  • MEMBRES DE LA BANQUE :
  • FREDERICO MONTEIRO FEIJÓ
  • IRAILDES PEREIRA ASSUNCAO
  • MARIA DE FATIMA SILVA MUNIZ
  • Data: 20 févr. 2020


  • Afficher le Résumé
  • O gênero Colletotrichum é considerado cosmopolita e tem sido um dos patógenos fúngicos mais importantes do mundo. Dentre as patologias que afetam a cultura do maracujazeiro, a antracnose é a principal doença acarretando grandes prejuízos socioeconômicos no nordeste brasileiro, no entanto, pouco se sabe sobre a etiologia da doença no Brasil. Portanto, os objetivos deste estudo foram identificar espécies do gênero Colletotrichum que infectam folhas de maracujazeiro, utilizando análises filogenéticas multi-locus associadas a características morfoculturais, bem como avaliar a virulência em gama de hospedeiros. Isolados do gênero Colletotrichum obtidos de folhas de maracujazeiro apresentado sintomas típicos da doença, foram coletados nos municípios de Coruripe, Quebrangulo e Maragogi. Os isolados patogênicos foram identificados com base nas sequências dos genes GAPDH, TUB2, CHS e região ITS-rDNA. Para caracterização cultural das espécies identificadas foram realizadas avaliações da taxa de crescimento micelial a 25 °C e aspecto das colônias. Na caracterização morfológica foram mensurados o comprimento e a largura de 50 conídios e apressórios. As análises filogenéticas multi-locus associadas a características morfoculturais de dessezete isolados revelaram seis espécies de Colletotrichum pertencentes a quatro complexos distintos: C. fructicola, C. theobromicola, C. tropicale (Complexo gloeosporioiodes), C. plurivorum (C. orchidearum), C. brevisporum (C. magnum), Colletotrichum sp. (C. boninense). Este é o primeiro relato de C. tropicale, C. fructicola e C. theobromicola no mundo e de C. brevisporum e C. plurivorum no Brasil. Todas as espécies de Colletotrichum foram patogênicas a gama de hospedeiros inoculadas (manga, maracujá, banana, mamão e goiaba), com excessão das espécies C. brevisporum, Colletotrichum sp. e C. plurivorum ao fruto de goiaba.

5
  • LIDIA RAFAELE ALMEIDA DA SILVA
  • DIVERSIDADE DE ÁCAROS PREDADORES COM ÊNFASE EM PHYTOSEIIDAE NA VEGETAÇÃO DO BIOMA CAATINGA DE ALAGOAS

  • Leader : EDMILSON SANTOS SILVA
  • MEMBRES DE LA BANQUE :
  • EDMILSON SANTOS SILVA
  • ELIO CESAR GUZZO
  • MÉRCIA ELIAS DUARTE
  • Data: 21 févr. 2020


  • Afficher le Résumé
  • Os ácaros são artrópodes quelicerados que compõe o segundo maior grupo depois dos insetos. São diversos e especializados em hábitos alimentares como fitófagos, micófagos, detritívoros e predadores. Dentre eles, os ácaros predadores são importantes agentes no controle natural de microartrópodes e ocorrem em diferentes ecossistemas. Atualmente, estes são utilizados em cultivos agrícolas, especialmente a família Phytoseiidae, para o controle biológico de pragas, como ácaros fitófagos, tripes e mosca-branca. Apesar da sua importância como agente de regulação biótica, ainda não se conhece, em alguns Biomas brasileiros como na Caatinga, o número de espécies que representar a riqueza e diversidade dos mesmos. Desta forma, o presente trabalho teve por objetivo conhecer a fauna de ácaros predadores em vegetação do Bioma Caatinga de Alagoas. Foram realizadas coletas no período de janeiro a agosto de 2019, no município de Olho D’água das Flores – AL, em um fragmento de mata remanescente do Bioma Caatinga, bem como em uma área de cultivo de palma forrageira (Nopalea cochenillifera (L.) Salm-Dyck). As cinco espécies de planta da vegetação natural selecionadas foram: catingueira (Poincianella pyramidalis [Tul.] L.P. Queiroz), juazeiro (Ziziphus joazeiro Martius), Marmeleiro (Croton blanchetianus Baill), Pereiro (Aspidosperma pyrifolium Mart.) e Braúna-do-Sertão (Schinopsis brasiliensis Engl.). A extração dos ácaros foi realizada com a observação de cada folha, utilizando-se microscópio estereoscópico. Os ácaros coletados foram montados em lâmina de microscopia e identificados utilizando chaves dicotômicas especializadas. Um total de 1922 ácaros foram encontrados distribuídos em dez famílias e uma sub-ordem, sendo seis (6) famílias de predadores (Bdellidae, Camerobiidae, Cunaxidae, Iolinidae, Phytoseiidae e Tydeidae,), com a identificação de 16 gêneros e 14 espécies. Phytoseiidae foi a família com maior riqueza de espécies (Amblyseius aerialis Muma 1955, Euseius alatus De Leon, 1966, Euseius concordis Chant 1959, Iphiseiodes zuluagai Denmark e Muma 1972, Typhlodromalus peregrinus Muma 1955, Transeius bellotti Moraes e Mesa 1998 e Neoparaphytoseius sp. n.), sendo T. bellotti e I. zuluagai as espécies mais abundantes e frequentes durante todo o período de estudo. Dentre os predadores encontrados foi descrita uma nova espécie de Neoparaphytoseius Chant e McMurtry 2003, classificado como Phytoseiidae. Ressalta-se que esta é a primeira espécie descrita para o Bioma Caatinga.

6
  • ISABELLE CRISTINA SANTOS MAGALHÃES
  • EXTRATO AQUOSO DE FOLHAS DE PINHEIRA NO MANEJO DA CASCA-PRETA-DO-INHAME

  • Leader : MARIA DE FATIMA SILVA MUNIZ
  • MEMBRES DE LA BANQUE :
  • EDNA PEIXOTO DA ROCHA AMORIM
  • MARIA DE FATIMA SILVA MUNIZ
  • MARYLIA GABRIELLA SILVA COSTA
  • Data: 28 févr. 2020


  • Afficher le Résumé
  • O inhame (Dioscorea spp.) é a quarta tuberosa mais produzida mundialmente e sua produção vem aumentando a cada ano, devido a sua excelente aceitação para a comercialização no mercado interno e externo, por obter uma gama de utilidades. Entre os problemas fitossanitários que contribuem para a baixa produtividade da cultura do inhame no Brasil, destaca-se a casca-preta causada por Scutellonema bradys, Pratylenchus coffeae e P. brachyurus, que incidem diretamente sobre os rizóforos, ocasionando a necrose nos tecidos. O presente trabalho teve por objetivo avaliar o efeito nematicida do extrato aquoso obtido de folhas de Annona squamosa no manejo da casca-preta-do-inhame em casa de vegetação. As plantas de inhame foram inoculadas 30 dias após o plantio, com suspensão de 1.000 espécimes dos nematoides S. bradys e Pratylenchus sp., com predomínio da primeira espécie, os quais foram aplicados ao solo.  Os experimentos foram conduzidos com delineamento inteiramente casualizado, em esquema fatorial 5 x 2 + 2, com sete repetições, sendo utilizadas cinco concentrações de extrato de pinha (1,0; 2,0; 3,0; 4,0 e 5,0 %), dois períodos de aplicação (30 e 60 dias após a inoculação), mais o controle negativo (água) e o controle positivo (Benfurocarbe Pottente®), onde a aplicação do extrato foi equivalente a 100 mL por planta nos períodos determinados. Seis meses após o plantio foi avaliado a população de nematoides no solo e raízes, e o fator de reprodução. Não houve diferença significativa para os períodos de aplicação do extrato em ambos os experimentos.  As análises de regressão foram melhor representadas pelo modelo raiz-quadrático e em todos os tratamentos houve redução da população dos nematoides, principalmente a partir da concentração de 3%. O extrato aquoso de folhas de A. squamosa apresentou efeito nematicida, mostrando-se eficiente no manejo das populações de S. bradys e Pratylenchus sp. 

7
  • TELLIANE SANTOS SALGUEIRO SILVA
  • Interação entre Cotesia flavipes (Cameron, 1891) (Hymenoptera: Braconidae) e Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) no parasitismo de Diatraea saccharalis (Fabr., 1974) (Lepidoptera: Crambidae).

  • Leader : ELIO CESAR GUZZO
  • MEMBRES DE LA BANQUE :
  • ELIO CESAR GUZZO
  • GERALDO PEREIRA DE ARRUDA FILHO
  • JUAN PABLO MOLINA ACEVEDO
  • Data: 28 févr. 2020


  • Afficher le Résumé
  • Nematoides entomopatogênicos (NEPs) são considerados organismos benéficos, porém, eles podem afetar insetos que também são benéficos, como os parasitoides. O objetivo deste trabalho foi avaliar a interação entre o endoparasitoide larval Cotesia flavipes (Cameron, 1891) (Hymenoptera: Braconidae) e o nematoide Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae), no controle da broca da cana-de-açúcar Diatraea saccharalis (Fabricius, 1974) (Lepidoptera: Crambidae), contribuindo com a tomada de decisão para o uso do controle biológico no manejo integrado de pragas (MIP) por parte dos produtores de cana-de-açúcar. Lagartas de D. saccharalis foram oferecidas para fêmeas de C. flavipes para parasitismo. Posteriormente, a cada 3 dias, foram inoculados NEPs. Após a mortalidade (que acontecia após o segundo dia de inoculação), esperou-se 4 dias para se fazer a dissecação de D. saccharalis. Os resultados mostram que NEPs são capazes de matar em 48 horas lagartas de D. saccharalis, matando também as fases de pupa e adulto do mesmo inseto. Os resultados ainda mostram que NEPs invadem o corpo de C. flavipes ainda dentro de D. saccharalis, impedindo assim o desenvolvimento do endoparasitoide. Pode se concluir com essa pesquisa que existe competição intraguilda entre os agentes de controle biológico, porem os mesmos podem ser usados no MIP, respeitando-se um período de carência entre a aplicação deles. Após a liberação do endoparasitoide C. flavipes, deve ser aguardado 9 a 12 dias para aplicação de NEPs, assim o desenvolvimento da mesma não será prejudicado aproveitando em campo a eficiência dos dois inimigos naturais.

8
  • FERNANDA EMANUELLE MENDONCA DE MORAIS
  • SELETIVIDADE DOS EXTRATOS ORGÂNICOS DE Annona muricata L. E Annona squamosa L.  AO PREDADOR Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae)

  • Leader : ROSEANE CRISTINA PREDES TRINDADE
  • MEMBRES DE LA BANQUE :
  • ROSEANE CRISTINA PREDES TRINDADE
  • MAURICIO SILVA DE LIMA
  • WENDEL JOSÉ TELES PONTES
  • Data: 28 févr. 2020


  • Afficher le Résumé
  • O controle biológico de insetos atua de forma equilibrada com o meio ambiente e mostra bastante eficiência quando combinado com outros métodos de controle. Estudos de seletividade de inseticidas botânicos sobre inimigos naturais são imprescindíveis para associar o controle biológico com o controle alternativo dentro do Manejo Integrado de Pragas (MIP). Dentre os agentes de controle biológico, do grupo predadores merecem destaque as espécies da família Coccinellidae, pois são conhecidos pela voracidade e por seu hábito alimentar generalista. Nesse contexto, o objetivo deste trabalho foi avaliar a seletividade dos extratos de Annona muricata L. e Annona squamosa L. sobre o predador Cryptolaemus montrouzieri Mulsant, 1853 (Coleoptera: Coccinellidae). Para isso, foram realizados bioensaios com 7 tratamentos: o extrato etanólico de graviola (EEG 0,23 e 1,19%)e extrato etanólico de pinha(EEP 0,39 e 5,47%), o produto formulado a base de acetogenina Anosom® e o produto comercial Decis 25 EC® ambos testadosna dose recomendada pelo fabricante, além de uma testemunha. Os produtos testados foram classificados seguindo os padrões de seletividade do Grupo de Trabalho Internacional com Organismos Benéficos e Pesticidas da International Organization of Biological Control (IOBC). Na ação dos extratos por contato o EEG 0,23 e 1,19%, e o EEP 0,39%foram considerados inócuos as larvas de quarto ínstar e aos adultos do predador, diferente do tratamento com Decis 25 EC® (tratamento controle positivo) que foi nocivo ao predador. Para o efeito residual todos os tratamentos, exceto o Decis 25 EC®, foram inócuos a larvas e adultos de C. montrouzieri. Quanto a seletividade dos extratos por ingestão o EEG 0,23 e 1,19% e o EEP 0,39%foram classificados como inócuo as larvas e adultos do predador, assemelhando-se ao tratamento com o Anosom®, diferente do EEP 5,47% que causou mortalidade em 30% das larvas de quarto instar e 40% dos adultos tratados, sendo classificado como moderadamente nocivo. O EEG foi o único tratamento classificado como inócuo para as duas fases de desenvolvimento avaliadas, nos três bioensaios realizados, apresentando-se como uma forma de controle alternativo compatível com o agente de controle biológico de C. montrouzieri.

9
  • YOLANDA DE MELO DE OLIVEIRA
  • DIVERSITY OF THE SPECIES COMPLEX Ralstonia solanacearum IN THE STATE

    OF ALAGOAS, BRAZIL

  • Leader : ADRIANO MARCIO FREIRE SILVA
  • MEMBRES DE LA BANQUE :
  • ADRIANO MARCIO FREIRE SILVA
  • MARIA DE FATIMA SILVA MUNIZ
  • GREECY MIRIAN RODRIGUES ALBUQUERQUE
  • Data: 28 févr. 2020


  • Afficher le Résumé
  • The bacterial wilt caused by species of Ralstonia solanacearum complex is considered one of
    the most destructive diseases in world agriculture, due to its wide geographical distribution,
    extensive range of hosts and difficult control. The use of resistant varieties, mainly rootstocks,
    is considered an efficient measure to control of bacterial wilt. However, the breakdown of
    resistance occurs frequently due to the high genetic and phenotypic variability found within
    the R. solanacearum species complex. Therefore, the genetic diversity of Ralstonia
    solanacearum (phylotype II) (55.3%) and R. pseudosolanacearum (phylotype I) (44.7%) were
    analyzed in a total of 103 isolates, obtained from symptomatic plants of banana, eggplant,
    pepper and tomato in the Agreste and Eastern mesoregions of the state of Alagoas, Brazil.
    Based on genotyping using REP markers, it was possible to observe the presence of 11 clonal
    lines among the 103 isolates analyzed, 22 of which were selected for sequencing of the egl
    gene. R. solanacearum prevailed in the Agreste mesoregion, where only isolates of sequevar
    IIA-6/35 were detected, which did not amplify the 220bp band specific to sequevar 6 by
    Mmx-PCR. R. pseudosolanacearum prevailed (53.9%) in the East mesoregion, with
    sequevars I-17 and I-18 being characterized. It is worth noting that R. solanacearum was also
    significantly found (46.1%) in this mesoregion, with the sequevars IIA-36, IIA-41, IIA-53
    being identified. This is the first work that reports the diversity of the R. solanacearum
    species complex in the state of Alagoas, Northeast region, Brazil.

10
  • CECILIA HERNANDEZ RAMIREZ
  • EFEITO DE PRODUTOS BIOLÓGICOS SOBRE NEMATOIDES CAUSADORES DA CASCA-PRETA-DO-INHAME

     

  • Leader : MARIA DE FATIMA SILVA MUNIZ
  • MEMBRES DE LA BANQUE :
  • EDNA PEIXOTO DA ROCHA AMORIM
  • JAQUELINE FIGUEREDO DE OLIVEIRA COSTA
  • MARIA DE FATIMA SILVA MUNIZ
  • Data: 11 sept. 2020


  • Afficher le Résumé
  • O inhame (Dioscorea spp.) é uma hortaliça cultivada, principalmente, em estados da região nordeste do Brasil. Uma das principais doenças que afetam a cultura é a casca-preta causada pelos fitonematoides Scutellonema bradys, Pratylenchus brachyurus e P. coffeae provocando necrose nos tecidos dos rizóforos, sendo necessário o uso de práticas que reduzam as perdas pelos patógenos. O presente trabalho teve por objetivos avaliar em ensaios in vitro e in vivo o efeito nematicida de produtos biológicos comerciais sobre os nematoides causadores da doença. Em placas de Kline foram testados Trichodermil® (Trichoderma harzianum) nas concentrações de 1,5 e 2,0 L produto/200 L de água, Presence® (Bacillus spp.), 100 e 150 g/100 L, Quartzo® (Bacillus spp.) e Bio-Ax® (Carbono orgânico), 5L e 7L/100L, além da testemunha, sobre S. bradys e Pratylenchus sp. Em casa de vegetação, rizóforos-sementes sadios foram cultivados em solo esterilizado e aos trinta dias as plantas foram inoculadas com uma suspensão de 1.000 espécimes de uma população mista formada por S. bradys e P. coffeae. Após 30 dias da inoculação foram aplicados ao solo os produtos Presence® 150 g/100L; Trichodermil® 2 L /200L; Bio Ax® 7 L/100L. Três meses após o plantio foram avaliados a brotação dos rizóforos e no quinto mês, a densidade populacional dos nematoides. A brotação dos rizóforos-semente foi de 100% em todos os tratamentos. Presence® e Trichodermil® causaram 89% e 61% de mortalidade em S. bradys respectivamente, nas maiores concentrações testadas. Em espécimes de Pratylenchus sp., destacou-se Bio-Ax®, apresentando 51% e 45% de mortalidade na maior e menor concentração, respectivamente. Em casa de vegetação, Presence® e Bio Ax® mostraram-se mais efetivos na redução da densidade populacional dos nematoides, comparados à testemunha.

11
  • MIGUEL ANGEL MARTINEZ GUTIERREZ
  • (cis)-JASMONA COMO INDUTOR DE MECANISMO DE DEFESA VEGETAL DE  Vigna unguiculata (L.) Walp. (Fabaceae) PARA RESISTÊNCIA A Aphis craccivora Koch 1854 (HEMIPTERA: APHIDIDAE).

  • Leader : HENRIQUE FONSECA GOULART
  • MEMBRES DE LA BANQUE :
  • GAUS SILVESTRE DE ANDRADE LIMA
  • HENRIQUE FONSECA GOULART
  • JOÃO GOMES DA COSTA
  • Data: 7 oct. 2020


  • Afficher le Résumé
  • O feijão caupi, Vigna unguiculata (L.) Walp. (Fabaceae), é uma leguminosa importante na dieta humana. Um  dos fatores limitantes para sua produção é o pulgão preto, Aphis craccivora  Koch 1854 (Hemíptera: Aphididae) , responsável pela transmissão do vírus do mosaico (Cowpea aphid borne mosaic virus, CABMV). Umas das ferramentas no manejo integrado dessa praga é o uso de variedades resistentes, semioquímicos e indutores de resistência, que são um método ambientalmente adequado para os agricultores. O projeto  tem como objetivo  estudar a função do (Z)-jamosne  na ativação do volátil da planta  mecanismo de defesa do feijão caupi para resistir aos ataques do pulgão preto. Para tanto, trabalhamos com 3 genótipos de feijão-caupi (Vita 7, , BRS-Guariba e BR19 Gurgueia) e também verificar se o uso desta ferramenta causa alguma diferença de produtividade da planta. O trabalho será realizado com a plantação, a criação de pulgão no laboratório, a avaliação da susceptibilidade de cultivares, aplicação de cis-jasmona para testes antibioses, recolhendo compostos  voláteis antes e após da infestação, realizar bioensaios de comportamento com o olfatometro de quatro braços  em relação ao pulgão preto e finalmente tornar a identificação dos produtos voláteis, utilizando cromatógrafo gasoso acoplado ao espectrômetro de massas (EM) e Cromatografia Gasosa acoplada a eletroantenografia (CG-EAG). Atualmente foram descobertas as propriedades semioquímicas da (Z)-jasmona (BIRKETT et al., 2000; BRUCE et al., 2003) .Este produto, além de ter revelado propriedades repelentes para alguns pulgões de clima temperado, também se mostrou atrativo aos seus inimigos naturais, notadamente joaninhas e parasitoides (BIRKETT et al., 2000) ativando desta forma o terceiro nível trófico, responsável pelo controle biológico dos fitófagos. Trabalhos atuais têm revelado o papel da Z-jasmona no controle de insetos praga (DEWHIRST et al., 2012; VIEIRA et al., 2013) Os resultados esperados neste projeto são: o cultivo de BR19 Gurguéia (comercial) junto com o Vita 7 que são considerados variedades suscetíveis nesta investigação, a indução de compostos orgânicos voláteis liberados pelas variedades Vita 7 e BR19 Gurgueia após a aplicação de (Z)- jasmona é esperada com ação de repelência em o pulgão preto A. craccivora significativamente repelente, além de identificar a atração de seus inimigos naturais.

SIGAA | NTI - Núcleo de Tecnologia da Informação - (82) 3214-1015 | Copyright © 2006-2024 - UFAL - sig-app-2.srv2inst1 29/03/2024 04:08