Banca de DEFESA: MARCILIANO DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARCILIANO DA SILVA
DATE: 10/05/2023
TIME: 08:00
LOCAL: Videoconferência
TITLE:

Use of zebrafish as a model system in the ecotoxicological evaluation of a surface river with socioeconomic importance for the metropolitan region of Maceió - AL

KEY WORDS:

fish, active biomonitoring, biomarkers, water quality, contaminants

PAGES: 73
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

There is a growing number of studies that point to the effects of anthropogenic activities on the environment, especially in aquatic ecosystems. The consumption demands of the world's population have negatively affected water resources, due to the reception of personal hygiene products, agricultural products, domestic and industrial effluents in bodies of water. In this study, the zebrafish (Danio rerio) model system was used to evaluate the environmental quality of a watershed in the metropolitan region of Maceió, in northeastern Brazil. Water samples were collected according to specific protocols in three different locations (P1, P2 and P3), between the source and the mouth of the Pratagy River, with different degrees of anthropization and in two periods, in March and June 2022. The samples were destined for the determination of the physical-chemical parameters of the water and of different classes of contaminants, as well as for the embryo-larval toxicity test with zebrafish (ZELT), during 144 hours, with the analysis of lethal and non-lethal biomarkers , cardiotoxicity and neurotoxicity. Contaminants of different classes were detected in the analyzed samples, some of them above the established limit, others prohibited for use in a large part of the globe, including Brazil, such as Dichlorodiphenyltrichloroethane (4.4 DDT), banned for decades. Regarding the mortality rate, only a significant increase was observed in the P3 of the first collection. The evaluation of the number of spontaneous movements, a biomarker of neurotoxicity, showed a difference between P3 and the negative control in the first collection and P2 and control in the second sampling. The data set evaluated corroborate the negative influence of anthropic activities practiced around the Pratagy river basin and that these can affect the survival and physiology of aquatic organisms, thus impacting this aquatic ecosystem.

 

BANKING MEMBERS:
Presidente - 1265581 - LAZARO WENDER OLIVEIRA DE JESUS
Interno(a) - 1544255 - TACIANA KRAMER DE OLIVEIRA PINTO
Interno(a) - 3140619 - VANESSA DORO ABDALLAH KOZLOWISKI
Externo(a) ao Programa - 1105255 - JERUSA MARIA DE OLIVEIRA AMORIM
Externo(a) à Instituição - DENIS MOLEDO DE SOUZA ABESSA - UNESP
Externo(a) à Instituição - THIAGO LOPES ROCHA - UFG