STUDY OF THE SEMIOCHEMICALS INVOLVED IN THE INTRASPECIFIC INTERACTION OF Euchroma gigantea
(L., 1758) (COLEOPTERA: BUPRESTIDAE) IN Pachira aquatica AUBL (MALVACEAE)
Buprestidae; Behavioral control; IPM; Pheromone; Antenna; Morphology; SEM. Munguba; VOCs
The beetle Euchroma gigantea (Linnaeus) (Coleoptera: Buprestidae) is a Neotropical species occurring in hot, dry, or humid climates. It is considered a pest that damages the support system of trees in the Malvaceae family, where the larvae feed on the cortical and subcortical tissues of the plant, causing its death. To date, there is no record of any control method registered with the Ministry of Agriculture, Livestock and Supply for the management of E. gigantea, making pheromones a useful tool for its control. This work aimed to identify the sex or aggregation pheromone of the species E. gigantea, to morphologically characterize, by scanning electron microscopy, the antennae of males and females, and to analyze the profile of volatile organic compounds present in leaves and branches of P. aquatica. Adult insects were manually collected from P. aquatica, and volatiles emitted by males and females were obtained by dynamic headspace and solvent extraction, while VOCs from leaves and branches of the plant were collected exclusively by dynamic headspace. Chemical analyses revealed the presence of 12 compounds in the extracts obtained by dynamic headspace, and 13 compounds identified in the solvent extraction. Electrophysiological studies indicated that four compounds (nonanal, decanal, 1-decyne, and 1,2- benzenedicarboxylate (2-methylpropyl)) showed biological activity against the beetle. Bioassays demonstrated that baits containing these compounds significantly attracted insects. Morphological characterization of the antennae revealed three types of sensilla in males and females: trichodeous, chaetic, and basiconic. Analysis of VOCs from leaves and branches of P. aquatica allowed the identification of 15 compounds, among which two compounds showed biological activity in males, corresponding to α-pinene and cis-ocimene.