Banca de DEFESA: JACKELINE LAURENTINO DA SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : JACKELINE LAURENTINO DA SILVA
DATE: 28/02/2024
TIME: 09:00
LOCAL: Mini-Auditório da Clínica Fitossanitária - CECA
TITLE:

SUSCEPTIBILITY OF Colletotrichum SPECIES ASSOCIATED WITH PASSION FRUIT ANTHRACNOSE USING CHEMICAL AND BIOLOGICAL CONTROL.

KEY WORDS:

Fungitoxic; Biofungicides; Passiflora.

PAGES: 60
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitossanidade
SPECIALTY: Fitopatologia
SUMMARY:

Passion fruit (Passiflora spp.) is one of the main tropical fruits grown in Brazil, in addition to being the world's leading producer of passion fruit. Anthracnose, caused by species of the genus Colletotrichum, is one of the main factors contributing to the reduction of production. Knowing the importance of this pathogen for culture and the great diversity of species of this fungal genus, it is essential to develop appropriate techniques to control the disease. Therefore, the objectives of this study were to evaluate in vitro and in passion fruit seedlings the effects of the fungicides Difenoconazole, Pyraclostrobin, Flutriafol and Azoxystrobin + Difenoconazole, Trichoderma harzianum and Bacillus spp. on Colletotrichum spp. In the in vitro evaluation of the sensitivity of Colletotrichum spp. to fungicides, the pathogens were inoculated in BDA culture medium supplemented with the concentrations established for each fungicide. In the in vivo evaluations, healthy seedlings of passion fruit from the Cerrado were used in preventive and curative treatments. Evaluating Trichoderma in the pairing test, filter paper discs moistened in the suspension of Colletotrichum spores (2x10⁶ conidia/mL) were used and placed on the edge of Petri dishes containing synthetic Potato-Dextrose-Agar (BDA – Kasvi) culture medium. After 72 hours, the same procedure was performed using Trichodermil SC 1306 ® (T. harzianum), at concentrations of 0.5; 1.0 and 1.5 L/ha, placing the moistened paper disc in the opposite direction to the phytopathogens.  The control was composed only of the plate with the phytopathogens. To evaluate the action of metabolites, plugs containing structures of each Colletotrichum species were placed in the center of Petri dishes, separately. After 48 hours, Trichoderma harzianum plugs were placed on plates containing synthetic PDA medium. The bases of the plates containing antagonist and phytopathogens were superimposed, joined and sealed. As a control, a base containing a mycelium disk containing the structures of the phytopathogens and the other containing only one disk of BDA culture medium was superimposed. For the antagonism test using Bacillus spp, pathogen plugs and bacterial growth were inoculated. To verify the difference between the treatments tested in chemical and biological control, the mycelial growth index (MCI) and the percentage of mycelial growth inhibition (ICP) were estimated. In general, the fungicide pyraclostrobin was the most efficient both in vitro and in vivo in the control of Colletotrichum spp. In addition, T. harzianum is a promising species for the reduction of anthracnose of anthracnose-associated species in passion fruit crops. The isolates of B. subtilis (R14 and IN937b) obtained the best results in all Colletotrichum species tested.

COMMITTEE MEMBERS:
Interno(a) - 1546023 - IRAILDES PEREIRA ASSUNCAO
Interno(a) - 2149632 - SARAH JACQUELINE CAVALCANTI DA SILVA
Externo(a) ao Programa - 1119045 - EDNA PEIXOTO DA ROCHA AMORIM - UFALExterno(a) ao Programa - 1121083 - MARIA DE FATIMA SILVA MUNIZ - UFALExterno(a) à Instituição - JULIANA PAIVA CARNAUBA - IFAL