Banca de DEFESA: YOLANDA DE MELO DE OLIVEIRA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : YOLANDA DE MELO DE OLIVEIRA
DATE: 26/02/2024
TIME: 14:00
LOCAL: Sala de aula do prédio da pós-graduação
TITLE:

Transmission, incidence and molecular characterization of Begomovirus associated with Phaseolus lunatus in the Northeast region.

KEY WORDS:

Geminiviridae, Fabaceae, whitefly.

PAGES: 60
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitossanidade
SPECIALTY: Fitopatologia
SUMMARY:

The genus Phaseolus belongs to the Fabaceae family, originating in the Americas, having around 55 species. Among these species, common bean (Phaseolus vulgaris) and lima bean (Phaseolus lunatus) are the most economically important and cultivated in Brazil. Beans play an important role in human nutrition, being a cheaper source of protein compared to meat, eggs and fish. The genus Begomovirus (family Geminiviridae) is among the most harmful pathogens that infect these crops, being considered a limiting factor in producing areas. In Brazil, the most economically important viruses that infect these hosts are Bean golden mosaic virus (BGMV) and Macroptilium yellow spot virus (MaYSV). Begomoviruses are transmitted by a complex of cryptic species of Bemisia tabaci, causing direct and indirect damage, limiting the production of this legume. In this context, the present study aims to identify, at the species level, the Begomoviruses that naturally infect P. vulgaris and P. lunatus collected in the Northeast region and to determine host attractiveness and virus transmission using Bemisia tabaci, Middle East-Asia Minor (MEAM1). Leaf samples of P. vulgaris and P. lunatus were collected in the states of Alagoas, Sergipe and Pernambuco, in Northeastern Brazil. Total DNA was extracted and used as a template for PCR using specific primers for BGMV and MaYSV, followed by amplification of viral genomes by rolling circle. These genomes will be commercially cloned and sequenced by primer walking. To correctly attribute taxonomy to the new isolates, paired comparisons of the complete genome with other previously reported begomoviruses and phylogenetic analysis will be used. A colony of B. tabaci will be established, which will be used in transmission and attractiveness tests with noncultivated plants. For attractiveness and transmission tests, the following species will be used: Senna occidentalis, Blainvillea latifolia, Macroptilium lathyroides, Hyptis suaveolens, Chenopodium album, Desmodium tortuosum, Phaseolus lunatus and Solanum lycopersicum. Heavily infested cabbages will be placed between the plants for a week, the infestation will be accounted for by counting the number of eggs, nymphs and adults present on the leaflets, other parameters will also be recorded, including the growth of sooty mold, the damage caused to the leaves and the nymph colonization. For the transmission test, whiteflies will be fed on a Macroptolium sp. plant, infected with begomovirus, for a period of 24 hours (acquisition access period) and then placed to feed on each species of plant used in the study, individually for 48 hours (period of access to inoculation). Initially, the presence of symptoms will be evaluated and, 30 days after infection, leaf samples will be collected for total DNA extraction and confirmation by PCR with species-specific primers.

COMMITTEE MEMBERS:
Presidente - 1546023 - IRAILDES PEREIRA ASSUNCAO
Interno(a) - 1790557 - GILDEMBERG AMORIM LEAL JUNIOR
Interno(a) - 2149632 - SARAH JACQUELINE CAVALCANTI DA SILVA
Externo(a) ao Programa - 3283002 - MILENY DOS SANTOS DE SOUZA - UFALExterno(a) à Instituição - FREDERICO MONTEIRO FEIJÓ - UFAL