Banca de QUALIFICAÇÃO: DENISE DE SANTANA SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : DENISE DE SANTANA SILVA
DATE: 25/03/2024
TIME: 09:00
LOCAL: CECA-UFAL
TITLE:

CHARACTERIZATION AND COMPARATIVE EPIDEMIOLOGY OF Colletotrichum SPECIES ASSOCIATED WITH ANTHRACNOSE IN Barbados cherry

KEY WORDS:

Barbados cherry; diversity; phylogeny; fungicide.

PAGES: 57
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitossanidade
SPECIALTY: Fitopatologia
SUMMARY:

Anthracnose is the most important disease in the cultivation of the acerola tree (Malpighia emarginata DC.), in almost all the states of the Northeast. The highest incidence of the disease is during the rainy season, which makes it an obstacle to the expansion of the crop. The disease is caused by several species of the genus Colletotrichum, the symptoms being lesions on leaves, branches, stems, flower buds, flowers and fruit. The species C. gloeosporioides is reported to be the etiological agent associated with anthracnose and the most widespread in acerola. The aim of this research was to identify Colletotrichum species on acerola through molecular and morphocultural characterization, cross-pathogenicity, as well as to evaluate the influence of temperature on the intensity of the disease and the effect of fungicides on the mycelial growth of these species. Isolates of Colletotrichum species were obtained from leaves and fruit showing typical symptoms of the disease, collected from plantations in the states of Alagoas, Bahia, Ceará, Paraíba, Pernambuco, Piauí, Rio Grande do Norte and Sergipe. A total of one hundred and eighty-five isolates representing these geographical regions were obtained. These isolates were subjected to PCR with an intergenic spacer between the DNA lyase (APN2) and MAT1-2-1 (ApMAT) locus genes, in order to identify which isolates amplified for the C. gloeosporioides complex. Of the total number of isolates, thirty-four were sequenced for the ApMAT region and a further six for the glyceraldehyde-3 phosphate dehydrogenase (GAPDH) gene. Subsequently, phylogenetic analyses were based on multilocus - chitin synthase (CHS-1), β-tubulin (TUB2), calmodulin (CAL), glutamine synthetase (GS) and the ITS-rDNA region. Cultural characterization was carried out by measuring the mycelial growth of the isolates at 25°C and aspects of the colonies. Morphological characterization was carried out by measuring conidia and appressoria. Multilocus sequence analysis identified three complexes of the genus Colletotrichum: gloeosporioides with the species C. theobromicola and C. chrysophilum; boninense complex with the species C. karstii and the acutatum complex. Cross-pathogenicity was carried out on varieties of acerola (Okinawa and Sertaneja), guava (cv. Paluma) and mango (Tommy Atkins). An experiment was carried out on the influence of temperatures of 10, 15, 20, 25, 30 and 35°C on mycelial growth in glass and the inhibition of mycelial growth by three commercial fungicide formulations: difenoconazole, azoxystrobin + difenoconazole and methyl thiophanate.

COMMITTEE MEMBERS:
Presidente - 1369412 - GAUS SILVESTRE DE ANDRADE LIMA
Interno(a) - 1065779 - ADRIANO MARCIO FREIRE SILVA
Interno(a) - 2149632 - SARAH JACQUELINE CAVALCANTI DA SILVA
Externo(a) ao Programa - 3339094 - MAYRA MACHADO DE MEDEIROS FERRO - UFALExterno(a) à Instituição - FREDERICO MONTEIRO FEIJÓ - UFAL