INTERACTIONS BETWEEN Rhynchophorus palmarum LINNAEUS, 1758 (COLEOPTERA: CURCULIONIDAE) AND Billaea rhynchophorae BLANCHARD, 1937 (DIPTERA: TACHINIDAE)
Arecaceae, Biological Control, Volatile Organic Compounds, Chemical Ecology.
Rhynchophorus palmarum Linnaeus, 1758 (Coleoptera: Curculionidae) is the main pest of arecaceae in the Neotropical region, occurring in several Brazilian states, attacking, in addition to palm trees, several cultures of economic importance. In coconut and oil palm, it causes direct and indirect damage, leading to reduced productivity. Considering that there is no chemical product registered to combat this pest, the most efficient control methods are cultural, behavioral and biological. Parasitoid flies of the genus Billaea Robineau-Desvoidy, 1830 (Diptera: Tachinidae) have been used to control pests of large crops in Latin America, and in Brazil the natural parasitism of Billaea spp. in oil palm plantations infested with R. palmarum, but there are no studies on the chemical ecology of these natural enemies. Thus, the present work aimed to identify Volatile Organic Compounds (VOCs) involved in the pest-parasitoid interaction, as well as the cuticular compounds of B. rhynchophorae Blanchard, 1937. The pest and the parasitoid were collected in oil palm plantations in the state of Bahia, transported to the Natural Resources Research Laboratory (LPqRN) and packed in BOD. The collection of volatiles of R. palmarum larvae and oil palm stem were obtained through the dynamic headspace technique, cuticular extractions of different parts of B. rhynchophorae were also performed using solvent. The headspace and cuticular extracts were analyzed and the compounds identified by means of Gas Chromatography coupled to the Flame Ionization Detector (GC-FID) and Gas Chromatography coupled to Mass Spectrometry (GC-MS). Chemical analyzes revealed the presence of 17 compounds in the aeration extracts and seven (7) in the cuticular extracts. In the electroantenography bioassays, three compounds present in the headspace were active for B. rhynchophorae females. The compounds identified in the cuticular extracts are similar between the analyzed parts and belong to the hydrocarbon class.