Banca de QUALIFICAÇÃO: MARIA HELLOÁ COSTA DE OLIVEIRA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : MARIA HELLOÁ COSTA DE OLIVEIRA
DATE: 29/08/2022
TIME: 14:00
LOCAL: Sala 4 do Programa de Pós-Graduação do CECA/UFAL
TITLE:

NEXT GENERATION SEQUENCING IN DETERMINING THE DIVERSITY OF BADNAVIRUS THAT INFECT TROPICAL FLOWERS IN THE NORTHEAST REGION

KEY WORDS:

Caulimoviridae, Zingiberales, NGS sequencing

PAGES: 54
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitossanidade
SPECIALTY: Fitopatologia
SUMMARY:

One of the most promising segments of the national agribusiness is floriculture, with an emerging market of profitability all over the world. Tropical flowers are attacked by different pathogens, including badnaviruses. Badnaviruses (Family Caulimoviridae) consist of a group of viruses with a double circular DNA genome, encapsulated in non-enveloped bacilliform particles, and represented mainly by mealybug strands. Diseases dangerous to the world by serious viruses are in serious genera in tropical crops important in tropical ones, including Brazil. To characterize badnavirus species that occur in tropical plants of the Cannaceae, Heliconiaceae, and Zingiberaceae families, they were collected in the poles that produce tropical plants in Alagoas, Pernambuco and Ceará. Subjected to badnavirus detection via PCR) using the universal pink primers for the genus, twelve samples (“Cost. by virus in the invalid virus infection. Once identified as infected with the virus in the episomal form, the RCA products were selected and will be sequenced by NGS. How SDT sequences and genome comparisons sequences will be approved and viral for sequence analysis and SDT genome comparisons for species demarcation. To confirm the presence of infections identified by the NGS and ensure the safety of the method, design PCR primers for each virus designed for full signatures for the full genome via primer walking sequencing.

BANKING MEMBERS:
Presidente - 2149632 - SARAH JACQUELINE CAVALCANTI DA SILVA
Interno - 1790557 - GILDEMBERG AMORIM LEAL JUNIOR
Externa ao Programa - 073.335.824-18 - MAYRA MACHADO DE MEDEIROS FERRO - UFAL