Banca de DEFESA: TIAGO SILVA LIMA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : TIAGO SILVA LIMA
DATE: 28/02/2023
TIME: 13:00
LOCAL: Campus de Engenharia e Ciências Agrárias - Prédio da Pós-Graduação Sala 04
TITLE:

IDENTIFICATION OF Colletotrichum spp. ASSOCIATED WITH ANTHRACNOSIS IN HELICONIA AND ASSESSMENT OF SENSITIVITY TO FUNGICIDES.

KEY WORDS:

chemical control,

multi-locus phylogeny

Heliconiaceae

PAGES: 63
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitossanidade
SPECIALTY: Fitopatologia
SUMMARY:

Diseases are an important obstacle faced by heliconia producers, causing significant losses. The anthracnose disease, for example, is caused by fungi of the genus Colletotrichum, responsible for causing damage due to the difficult control, since the symptoms make the flower stems unsuitable for commercialization. Considered as an important plant pathogen, the genus Colletotrichum has a wide diversity of species, being responsible for causing a variety of diseases in several economic crops and ornamental plants, making it difficult to control this disease. The understanding and knowledge of which species of Colletotrichum are occurring in heliconia, as well as the infectious behavior of these species in different hosts and the effect of different fungicides on them is important for the development of more efficient control strategies. However, identification studies of this genus in heliconias are still scarce. Therefore, the objective of this work was to identify species of the genus Colletotrichum associated with heliconias through the use of molecular tools and morphocultural characteristics, as well as to evaluate the effect of different fungicides on mycelial growth and conidia germination. Fungal isolates were obtained from heliconia plants with anthracnose symptoms, in the states of Alagoas, Ceará and Pernambuco. These isolates will be characterized through multi-locus phylogenetic analysis and mold-cultural characters of the species. In vitro tests with the fungicides (Azoxistrobina + difenoconazol), (Clorotalonil), (Fluxapiroxade + Piraclostrobina) and (Difenoconazole) will be carried out to evaluate the inhibition of mycelial growth by the method of incorporation of fungicides in the PDA culture medium and the inhibition of conidia germination using excavated slides containing water with fungicide. Thirty-two isolates were obtained so far from flowers, leaves, petioles and bracts showing typical symptoms of anthracnose. The initial analysis of the partial sequences of the GAPDH gene was performed with seventeen isolates of Colletotrichum, the sequences were compared with the accesses of the GenBank database using the BLASTn algorithm, together with the construction of Bayesian Inference trees, it was possible to group the isolates in four complexes, two isolated in the C. boninense complex, twelve in the C. gloeosporioides complex, two in the C. magnum complex and one in the C. gigasporum complex. All isolates were able to develop symptoms on inoculation with wounds, inducing depressed, waterlogged, light brown, and variable sized necrotic lesions on leaves. With the study it is expected to know the diversity of species of Colletotrichum associated with the heliconia culture, obtaining relevant information about the pathosystem for the development of adequate strategies for the control of the disease.

BANKING MEMBERS:
Externo(a) ao Programa - 1119045 - EDNA PEIXOTO DA ROCHA AMORIM
Presidente - 1546023 - IRAILDES PEREIRA ASSUNCAO
Externo(a) à Instituição - JULIANA PAIVA CARNAUBA - IFAL
Externo(a) ao Programa - 1121083 - MARIA DE FATIMA SILVA MUNIZ
Interno(a) - 2149632 - SARAH JACQUELINE CAVALCANTI DA SILVA