Banca de DEFESA: IARA MARIA DOS SANTOS COSTA LUNA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : IARA MARIA DOS SANTOS COSTA LUNA
DATE: 16/02/2023
TIME: 14:00
LOCAL: Sala 4 do Programa de Pós-Graduação do CECA/UFAL
TITLE:

GENETIC DIVERSITY OF BEAN BEAN-ASSOCIATED BEGOMOVIRUS CULTIVATED IN A FAMILY PRODUCTION SYSTEM IN ALAGOAS

KEY WORDS:

Geminiviridae, Genetic variability, Phaseolus lunatus

PAGES: 41
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitossanidade
SPECIALTY: Fitopatologia
SUMMARY:

Beans are a staple food of fundamental importance for food and nutritional security as a
source of protein, in addition to income for small producers in Alagoas. Practically all of
the bean production in the State of Alagoas comes from family farming, which it uses for
its own consumption and sells its surplus, supplying the demands of the domestic market.
Production is based on the cultivation of common bean (P. vulgaris) and broad bean (P.
lunatus), and to a lesser extent, cowpea (Vigna unguiculata) and pigeonpea (Cajanus
cajan). The productivity of these crops is considered low, mainly due to the low adoption
of technology and the occurrence of phytosanitary problems, with emphasis on viruses
that belong to the genus Begomovirus (Family Geminiviridae). These have limited
production and reduced grain quality in bean crops grown in all areas of Brazil. Several
begomoviruses have been reported naturally infecting cultivated and non-cultivated
legumes, but Bean golden mosaic virus (BGMV) and Macroptilium yellow spot virus
(MaYSV) are the most economically important in the country. These species occur in
Alagoas since 2005 in bean and broad bean crops, and non-cultivated plants such as
Macroptilium spp., with MaYSV being the prevalent virus. In this context, the objective
of this research is to determine the genetic diversity of begomoviruses associated with P.
lunatus in a family production system in Alagoas. Leaf samples of P. lunatuss were
collected in different areas of family cultivation in the state of Alagoas. The total DNA
of each sample was extracted and used, initially, as a template for detection of
begomoviruses, using degenerate primers. Subsequently, the detection of the genomic components DNA-A and DNA-A of BGMV and MaYSV was performed using species-specific primers. Samples that tested negative for BGMV and MaYSV begomoviruses had their genomic components amplified via RCA, cleaved with restriction enzymes, ligated into the pBluescript KS+ vector and cloned into Escherichia coli DH10B. Clones containing recombinant vectors were selected for primer walking sequencing. 2 DNA-B clones were obtained and subjected to whole-genome pairwise analysis with otherpreviously reported begomoviruses. These were more closely related to the species BGMV BR:Pai8:11 from Palmeira dos Índios - AL, with 95.72% nucleotide identity. However, it was not possible to correctly attribute taxonomy to the new isolates, due to the absence of DNA-A. The phylogenetic relationship between the characterized isolates was determined by reconstructing Bayesian inference phylogenetic trees for the DNA-A and DNA-B genomic component dataset. Possible recombination events and sites between the begomoviruses found and others stored in databases will also be determined.

BANKING MEMBERS:
Interno(a) - 1790557 - GILDEMBERG AMORIM LEAL JUNIOR
Interno(a) - 1407179 - ROBERTO RAMOS SOBRINHO
Interno(a) - 2149632 - SARAH JACQUELINE CAVALCANTI DA SILVA
Externo(a) ao Programa - 073.335.824-18 - MAYRA MACHADO DE MEDEIROS FERRO - IFPI