Banca de QUALIFICAÇÃO: MAYARA OLIVEIRA DE LIMA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : MAYARA OLIVEIRA DE LIMA
DATE: 31/08/2023
TIME: 13:30
LOCAL: Sala 4 do programa de pós-graduação em Proteção de Plantas
TITLE:

INCIDENCE AND CHARACTERIZATION OF BADNAVIRUS CAUSING BANANA STREAK DISEASE IN NORTHEAST BRAZIL

KEY WORDS:

Banana; BSOLV, BSGFV, Caulimoviridae, Musa spp., - genetic diversity

PAGES: 49
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitossanidade
SPECIALTY: Fitopatologia
SUMMARY:

Brazil is the fourth largest producer of bananas in the world, with emphasis on the Northeast Region, which contributes with about 40% of national production. However, the productivity of the crop in the country is considered low, being attributed mainly to phytosanitary problems. The main form of banana crop propagation is by mass micropropagation, which contributes to the accumulation of pathogens, especially viruses. Banana streak is one of the most important viruses of the crop, caused by a complex of nine different species of Banana streak virus (BSV) [genus Badnavirus, family Caulimoviridae], which are also transmitted by mealybugs (Pseudococcidae). Although the disease has been present in Brazil for years, studies on the diversity and variability of badnaviruses occurring in banana trees are scarce. Thus, this work aimed to determine the incidence and characterization of badnaviruses that infect banana trees in northeastern Brazil and to obtain the complete genome through high-throughput sequencing using the Illumna platform. Universal primers for the genus Badnavirus and species-specific primers for detection of BSOLV, BSGFV and BSIMV were tested. As a result, a high incidence of badnavirus infection was observed in all Northeastern states where samples were analyzed, but BSIMV infection was not detected. Amplification of the circular genome was obtained through amplification via RCA and its products sent for sequencing at LAcTAD (UNICAMP).

BANKING MEMBERS:
Presidente - 2149632 - SARAH JACQUELINE CAVALCANTI DA SILVA
Interno(a) - 1790557 - GILDEMBERG AMORIM LEAL JUNIOR
Externo(a) ao Programa - 3339094 - MAYRA MACHADO DE MEDEIROS FERRO