Banca de QUALIFICAÇÃO: EUZANYR GOMES DA SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : EUZANYR GOMES DA SILVA
DATE: 30/08/2023
TIME: 09:00
LOCAL: Google meet
TITLE:

DIVERSITY AND MYCOTOXIGENIC POTENTIAL OF SPECIES OF
FUSARIUM FUJIKUROI COMPLEX ASSOCIATED WITH BANANA CROWN ROT

KEY WORDS:

aggressiveness; fumosinin; post harvest diseases;

PAGES: 55
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitossanidade
SPECIALTY: Fitopatologia
SUMMARY:

Banana (Musa spp.), is one of the most explored fruits in Brazil and in the world and is
consumed worldwide due to its nutritional importance. However, its commercialization
is seriously affected by post-harvest rots that cause considerable damage and losses. The
disease is associated with fungal complexes, especially as Fusarium is one of the most
important fungal genera of plant pathogens that affect the cultivation of a wide range of
crops and produce various mycotoxins. Several species have already been reported as
pathogenic of crown rot, among these species are within the Fusarium fujikuroi species
complex (FFSC) which are major producers of mycotoxins, mainly fumonisins (FBs) and
of great clinical importance. Thus, the objective of the study is to characterize the FFSC
species with a molecular approach of isolates of banana crown rot, collected in shops and
processing units in the Cariri region of Ceará, to identify their pathogenicity and to
evaluate their production potential. of mycotoxins through identification of FUM genes
using specific primers in PCR analysis. To identify the Fusarium species, obtained from
crown rot were subjected to DNA extraction and sequenced for two gene regions (TEF
and RPB2). After identification, the isolates were tested for the presence of genes
involved in the production of fumonisins using specific primes (FUM53F/FUM53F and
VERT1/VERT2) and then the pathogenicity and aggressiveness of the isolates were
evaluated. Phylogenetic analyzes characterized 64 isolates belonging to the FFSC,
showing that the most frequent species were F. annulatum, F. musae and F. sacchari,
after F. verticillioides and F. pseudocircinatum. This is the first report of the F. annulatum
species associated with crown rot in the world. All isolates were analyzed for the presence
of the FUM1 and FUM8 genes. Nine isolates were positive for the genes, the species F.
verticillioides, F. musae and F. sacchari showed the presence of the FUM genes, having
the ability to produce fumosinin. All species were pathogenic, except for F. verticillioides.
There were significant differences in relation to the aggressiveness of the isolates, six
isolates were more aggressive identified as F. musae, F. annulatum and F. sacchari. This
study brings new contributions on the diversity of etiological agents associated with
crown rot in the Cariri region of cearense, FFSC members, and the ability of these species
to produce fumonisins. Considering the relevance that banana is one of the fruits most
present in the diet of the Brazilian population, our results contribute with new knowledge
for the future evaluation of quantification of mycotoxins in banana fruits in Brazil. Since,
this study indicates a potential threat to the quality of Brazilian banana.

BANKING MEMBERS:
Interno(a) - 054.821.814-56 - KAMILA CÂMARA CORREIA - UFRPE
Interno(a) - 2149632 - SARAH JACQUELINE CAVALCANTI DA SILVA
Externo(a) ao Programa - 3252041 - MARIOTE DOS SANTOS BRITO NETTO
Externo(a) à Instituição - SAMI JORGE MICHEREFF - UFCA
Externo(a) à Instituição - Willie Anderson dos Santos Vieira - UnB