Banca de QUALIFICAÇÃO: JOYCE CAMILA BARBOSA DA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : JOYCE CAMILA BARBOSA DA SILVA
DATE: 15/07/2022
TIME: 14:00
LOCAL: Sala virtual (https://meet.google.com/xhr-rmgo-kwr)
TITLE:

ISOLATION AND IDENTIFICATION OF CONTAMINATING FILAMENTAL FUNGI IN OPEN TREATMENT SYSTEM BY MICROALGAE

KEY WORDS:

whey, microalgae, fungus, bioremediation

PAGES: 36
BIG AREA: Engenharias
AREA: Engenharia Ambiental
SUBÁREA: Saneamento Básico/Esgotos/Reator Anaeróbico
SUMMARY:

Inadequate disposal of effluents from the manufacturing units of dairy industries can degrade the environment due to the high content of pollutants present in whey. In this way, it is necessary to pre-treat this effluent before being released into a water body, however, the physical-chemical treatments usually applied to this type of effluent are highly expensive, making it unfeasible for small and medium-sized industries. In this context, biological processes are highlighted due to the simplicity of their application and economic viability. Compared to traditional biological treatment strategies, the use of microalgae has advantages such as greater nutrient consumption capacity, on the other hand, there are some technical difficulties that prevent them from industrializing because the harvesting of microalgae cells is an economically challenging step due to to their small size, motility, negatively charged surfaces, and low cell densities. In view of this, the symbiotic relationship between algae and fungi is highlighted because this association facilitates the harvesting of algae by copelleting them into fungal pellets. Therefore, the present work aims to isolate and characterize filamentous fungi that contaminate the whey treatment process by microalgae of the species Tetradesmus obliquus LCE-01 in an open system in order to understand this dynamic. For the quantification and isolation of filamentous fungi, the serial dilution technique will be used in a laminar flow hood for spreading plating (Drigalsky loop), in YPDA (Yeast Peptone Dextrose) media (10 g/L of yeast extract, 20 g /L dextrose, 20 g/L peptone and 20 g/L agar) at pH 5.6; Nutrient Agar (1 g/L Meat Extract, 2 g/L Yeast Extract, 5 g/L Peptone, 5 g/L Sodium Chloride and 20 g/L Agar) at pH 6.8; Sabourard Agar (40 g/L dextrose, 10 g/L peptone, 20 g/L agar) at pH 5.6; Potato dextrose agar (PDA – Potato dextrose agar) (infusion water of 250 g of potato, 20 g/L of dextrose, 15 g/L of agar) at pH 5.6. It is expected to obtain fungal isolates that have symbiosis with Tetradesmus obliquus and increase the laboratory's culture bank for future biotechnological applications.

BANKING MEMBERS:
Presidente - 3081569 - CARLOS EDUARDO DE FARIAS SILVA
Externa ao Programa - 1296115 - ALBANISE ENIDE DA SILVA
Externa ao Programa - 1456420 - RENATA MARIA ROSAS GARCIA ALMEIDA