Banca de DEFESA: JENIFFER MCLAINE DUARTE DE FREITAS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JENIFFER MCLAINE DUARTE DE FREITAS
DATE: 28/07/2021
TIME: 08:00
LOCAL: https://meet.google.com/wcz-cavp-uog
TITLE:

PREPARATION AND CHARACTERIZATION OF MICROCAPSULES CONTAINING GREEN-BROWN PROPOLIS EXTRACT FROM THE UNIÃO DOS PALMARES-ALAGOAS

KEY WORDS:

Greenish-brown Propolis; Microcapsules; Spray-Dryer; Phenolic compounds; Antioxidant activity; Antibacterial Activity.

PAGES: 72
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

Propolis is a resinous substance of complex composition, formed by gummy and balsamic material, collected by bees (Apis mellifera) from different types of trees. Propolis has been the object of pharmacological studies due to its antibacterial, antifungal, antiviral, antiinflammatory, hepatoprotective, antioxidant, antitumor and immunomodulatory properties. Microencapsulation allows preventing the loss of substances by oxidation or volatilization, in addition to controlling the release of aromas, loss of nutrients and preserving the color and flavor of the encapsulated agent. In this work, the composition, chemical and biological properties and microencapsulation using Spray Dryer of greenish-brown propolis from União dos Palmares-Alagoas were studied for the first time. The objective of this work was to prepare and characterize microcapsules with greenish-brown propolis extract and evaluate the multiple constituents present, these analyzed by analytical methods such as UV-vis, UFLC-DAD-UV, GCMS, FTIR, SEM, Thermal Analysis , content of flavonoids and total phenols, antioxidant activity and antibacterial activity. The greenish-brown propolis extract and the microcapsules containing greenish-brown propolis extract from Alagoas presented a total flavonoid content of 5.15% and 1.13%, total phenols of 14.16 and 4.81% in in terms of gallic acid, total phenols of 16.53% and 6.00% in terms of catechin, antioxidant activity with IC50 values of 13.77 µg/mL and 60.80 µg/mL, respectively. Ultra-Efficiency Liquid Chromatography (UFLC-DAD-UV-Vis) identified the presence of chlorogenic acid, caffeic acid, ferulic acid, p-coumaric acid, naringenin, luteolin and kaempferol. The SEM and FTIR data showed encapsulation of phenolic compounds from the greenish-brown propolis from Alagoas and the thermal analysis showed the thermostability of the studied extracts. The dichloromethane fractions enriched with flavonoids from the crude extract and microcapsules demonstrated antibacterial activity against Staphylococcus aureus (ATCC 33591) and Providencia rettgeri (ATCC 29944). The data from this work demonstrate the potentials and biological properties of the greenish-brown propolis from Alagoas, as well as its spray-dryer solid products. 

BANKING MEMBERS:
Externo à Instituição - ABEL BARBOSA LIRA NETO - UNEAL
Externa ao Programa - 1120536 - GISELDA MACENA LIRA
Presidente - 1488396 - TICIANO GOMES DO NASCIMENTO