Banca de QUALIFICAÇÃO: MIKAELE MONIK RODRIGUES INACIO DA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : MIKAELE MONIK RODRIGUES INACIO DA SILVA
DATE: 21/03/2024
TIME: 10:00
LOCAL: PPGCS
TITLE:

ANALYSIS OF THE INFLUENCE OF POLYMORPHISMS IN THE TLR1, TLR4, TLR7, TLR8, TLR9 AND TIRAP GENES ON THE SEVERITY OF PLASMODIUM VIVAX INFECTIONS

KEY WORDS:

malaria, genetic variability, SNPs, Toll Like Receptor, TIR Domain Containing Adaptor Protein.

PAGES: 45
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

Malaria is a widespread infectious disease caused by protozoa of the genus Plasmodium and transmitted to the host by the bite of the female mosquito of the genus Anopheles. Malaria caused by P. vivax is characterized by mild initial symptoms and occurs after the onset of the mosquito bite within a period of 7 to 10 days. During the immune response to P. vivax infection, pro-inflammatory cytokines are released into the bloodstream of the host. The release of pro-inflammatory cytokines at the beginning of the disease is important for the control and elimination of the parasite. Toll-like receptors (TLRs) are transmembrane proteins present in epithelial, endothelial, natural killer, monocyte, macrophage, neutrophil, and dendritic cells. TLRs induce the production of pro-inflammatory cytokines by activating the NF-κB transcription factor in individuals infected with different infectious agents, including P. vivax. The main objective of this study is to assess whether the rs4833095, rs1927911, rs179008, rs3764880, rs352140, rs8177374 polymorphisms in the TLR1, TLR4, TLR7, TLR8, TLR9, and TIRAP genes of the immune system are associated with the clinical manifestations of malaria caused by P. vivax in a population from the Amazon region of Brazil. The study consisted of samples collected in the Amazon region from 216 individuals infected with P. vivax from the state of Pará. The patients were diagnosed using the blood smear microscopy technique and the levels of parasitemia and gametocythemia were estimated. The clinical symptoms of the individuals were assessed by the doctors and the patients were treated according to the recommendations of the Ministry of Health. The DNA samples were genotyped by allelic discrimination using the real-time PCR technique for TLR polymorphisms. The individuals were genotyped using a validated set of 48 ancestry-informative molecular markers for genomic control in the statistical analyses. The allele and genotype frequencies of the polymorphisms investigated will be estimated by direct counting, and the deviation of these frequencies from the Hardy-Weinberg equilibrium will be investigated by the Chi-square test. A principal component analysis will be used to group the symptoms presented by the patients into a clinical severity index. The genotypes of the TLRs will be analyzed for their association with the levels of parasitemia, gametocytaemia, and the clinical index by Logistic Regressions and Generalized Linear Models. Other statistical tests may be carried out depending on the distribution of the variables in the sample.

COMMITTEE MEMBERS:
Externo(a) à Instituição - LUCIANA TOVO RODRIGUES - UFPel
Interno(a) - 2119250 - MULLER RIBEIRO ANDRADE
Presidente - ***.650.590-** - VINICIUS DE ALBUQUERQUE SORTICA - UFRGS