Banca de QUALIFICAÇÃO: BARBARA RAYSSA CORREIA DOS SANTOS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : BARBARA RAYSSA CORREIA DOS SANTOS
DATE: 22/07/2021
TIME: 09:00
LOCAL: Virtual
TITLE:

Association of IL6 T15A (rs13306435) and INOS -1173 C>T (rs9282799) polymorphisms with susceptibility to infection and progression of dengue.

KEY WORDS:

Dengue. Interleukin 6. Nitric oxide. Genetic Polymorphism.

PAGES: 78
BIG AREA: Ciências Biológicas
AREA: Genética
SUMMARY:

Background: Dengue is a public health problem. Millions of cases are reported
worldwide. The infection with dengue virus has a variety of signs and symptoms
including asymptomatic and symptomatic cases. The immune system it is involved in
the host's response to infections as well as in the pathogenesis of diseases, including
dengue. Cytokines and nitric oxide are components of the immune system.
Interleukin 6 is a cytokine that plays an important role in linking the innate immune
response to the acquired immune response. iNOS is an enzyme responsible for
inhibiting viral replication through the production of nitric oxide. Objective: To
investigate the relationship of polymorphisms in the inducible nitric oxide (-1173 C /
T) and interleukin 6 (T15A) genes with dengue infection and disease progression.
Methodology: It was a case-control study with patients infected with dengue virus
and healthy controls of the population. The genotypes of the iNOS (-1173 C / T) and
cytokine IL-6 (T15A) genes were determined by the real-time polymerase chain
reaction (qPCR), using the allelic discrimination method. The analyzes of the correlations between genotypic and allele frequencies concerning susceptibility to infection were obtained using the SNPstats and BioEstat 5.3 software. Results: The study was composed of a total of 179 individuals, 27 composing the dengue fever group, 33 to the dengue hemorrhagic fever group, and 119 composing the control group. For the T15A polymorphism of the IL-6 gene, no difference was found in the llelic and genotypic distribution between the groups. For the -1173 polymorphism of the iNOS gene, no difference was found in the allelic and genotypic distribution between the groups. Conclusion: No association was found between the polymorphism of IL-6 T15A (rs13306435) and iNOS -1173C / T (rs9282799) with dengue infection.

BANKING MEMBERS:
Presidente - 1867374 - ELAINE VIRGINIA MARTINS DE SOUZA FIGUEIREDO
Interna - 2269479 - CAROLINNE DE SALES MARQUES
Externa à Instituição - ALINE CRISTINE PEREIRA E SILVA