Banca de QUALIFICAÇÃO: ANA LUCIA MENDES DA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ANA LUCIA MENDES DA SILVA
DATE: 15/12/2021
TIME: 09:00
LOCAL: Videoconferência
TITLE:
Uvaol effects on trophoblast cells in the chorioamnionitis induced by group B streptococcus

KEY WORDS:
Placenta; Inflammation; Choriomanionite; Group B streptococcus; Uvaol

PAGES: 89
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUBÁREA: Citologia e Biologia Celular
SUMMARY:
serious complications during pregnancy, such as maternal sepsis, chorioamnionitis,
prematurity, neonatal sepsis and neurodevelopmental disorders. GBS-induced
chorioamnionitis leads to changes in trophoblastic cells, with the production of
inflammatory mediators that can modify the way they interact within the placental
microenvironment, especially with endothelial cells, causing the modification of the
placental vascular structure in pregnant women due to production abnormal
angiogenic mediators. There is no prophylaxis for the deleterious effects caused by
GBS infection. Knowing that maternal nutrition directly influences pregnancy, we
evaluated whether uvaol, a triterpene present in olive oil, could be used preventively,
using different in vitro models. Objective: To investigate whether uvaol prevents
cellular changes caused by GBS incubation in vitro. Methodology: The HTR-8SV/neo
cell line and chorionic villi explants were pre-treated with uvaol and exposed to a non
lethal concentration of inactivated GBS (106 CFU), confirmed by MTT and labeling by
annexin V and propidium iodide. HTR-8SV/neo cells were evaluated for their
biochemical composition by Raman spectroscopy; morphology using phalloidin
fluorescein labeling; production of mitochondrial reactive oxygen species; cytokine
production by flow cytometry; phagocytosis by Giemsa staining; migration by in vitro
wound assay; endothelial invasion by 3D coculture with Ea.hy926 cells; and production
of angiogenic factors by flow cytometry. Placental explants were evaluated by MTT
and their production of angiogenic factors by flow cytometry. Results: Despite not
altering the viability of HTR-8SV/neo cells, GBS modified the cytoskeleton and
biochemical composition. GBS increased the production of IL-1β (p<0.05) and IFN-γ
(p<0.001) and oxidative stress, in addition to promoting phagocytosis. Cell migration
and invasion were reduced after incubation with GBS. Exposure to GBS led to
increased production of CXCL-8 (p<0.01) and IL-6 (p<0.05) in the 3D endothelial
invasion model, while uvaol prevented this increase (both p<0.05). With respect to
placental explants, the same effect was observed. Treatment with Uvaol proved to be
effective in preventing most of the changes caused by incubation with GBS, with an
important prophylactic potential. Conclusions: Even at a non-lethal concentration, the
presence of inactivated GBS caused inflammation, reduced trophoblast motility and
increased production of CXCL-8 and IL-6, factors that participate in vascular
dysregulation observed in several diseases and that can be triggered by placental GBS
infection. Due to its protective effect, it is possible that Uvaol is an alternative to prevent
the harmful effects caused by GBS in the maternal-fetal interface. 

BANKING MEMBERS:
Presidente - 2151027 - ALEXANDRE URBAN BORBELY
Interno - 2579081 - DANIEL LEITE GOES GITAI
Interna - 2270384 - MARIA DANIELMA DOS SANTOS REIS
Notícia cadastrada em: 06/12/2021 12:19
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