Banca de QUALIFICAÇÃO: THIAGO PINA GOES DE ARAUJO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : THIAGO PINA GOES DE ARAUJO
DATE: 19/08/2022
TIME: 09:00
LOCAL: https://meet.google.com/grj-pzpw-csu (video confeência)
TITLE:

ANTITUMOR ACTIVITY OF PIROLHENUS EXTRACTS ON HUMAN GLIOBLASTOMA CELLS (GBM02), IN VITRO

KEY WORDS:

Anti-tumor activity. Glioblastoma multiforme. Pyrolysis

PAGES: 72
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

 

Glioblastoma (GBM) is an extremely aggressive type of cancer that originates in the Central Nervous System (CNS) associated with high rates of morbidity and mortality in adults over 45 years of age. The gold standard treatment consists of tumor resection, followed by radiotherapy and adjuvant chemotherapy. Even after treatment, the short median survival is only 15 months, which does not contribute to a good prognosis of cure for affected patients. Thus, it is necessary to search for new substances with antitumor activity for GBM cancer cells. The pyroligneous extract (EP) comes from “pyrolysis”, a process that uses plant biomass in the thermal decomposition in the absence of oxygen, which has bioactive metabolites of important pharmacological interest, mainly antitumor. Therefore, the present study evaluated the in vitro antitumor activity of plant extracts from pyrolysis on human glioblastoma cells (GBM02). To evaluate the effects of extracts on GBM02 and blood mononuclear cell viability, the MTT colorimetric assay was used. Then, the selectivity of the derivatives was calculated through the ratio between cytotoxic concentrations for 50% of monocytes and lymphocytes (CC50) and inhibitory for 50% of GBM02 (CI50). Subsequently, the anti-migratory effect on GBM02 after the treatments was observed by the scratch assay. The morphological analyzes of GBM02 were carried out by means of optical microscopy. The results were statistically analyzed by one-way ANOVA followed by Dunnett's post-test, compared to the control group. All extracts showed a cytotoxic effect on GBM02 cell culture in MTT assays, among these, 4 (Cassava 550, Ouriricuri 700, Casca de Coco and Jurema Roxa) showed a higher selectivity index to glioblastoma than blood mononuclear cells. Morphological changes were also seen in the treatments with the 4 pyrolysis extracts. There are indications that the phenolic compounds present in greater amounts in the pyrolysis extracts are responsible for the morphological changes and cytotoxic effect observed in the experiments. Based on the potential of pyroligneous extracts to promote structural changes in GBM02 cell culture in vitro, pyrolysis extracts present themselves as great candidates for new research in search of drugs with pharmacological activity in the treatment of glioblastomas and it is necessary to carry out further investigations. additional tests in order to better elucidate the mechanisms of action of the extracts on the effects presented in GBM02.

BANKING MEMBERS:
Presidente - 1358530 - MAGNA SUZANA ALEXANDRE MOREIRA
Interna - 2272670 - ALINE CAVALCANTI DE QUEIROZ
Externa à Instituição - ANDRESSA LETICIA LOPES DA SILVA