Banca de DEFESA: CARMELITA BASTOS MENDES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CARMELITA BASTOS MENDES
DATE: 11/11/2022
TIME: 14:00
LOCAL: ICBS - Sala 19
TITLE:

Thyroid hormones as possible co-regulators of aquaporins 1 and 4 in human gliomas: a pilot study using a bioinformatics approach.

KEY WORDS:

glioma, AQP1, AQP4, biomarker, gene expression, correlation analysis, bioinformatics 

PAGES: 110
BIG AREA: Ciências Biológicas
AREA: Fisiologia
SUBÁREA: Fisiologia de Órgãos e Sistemas
SPECIALTY: Neurofisiologia
SUMMARY:

In 2016 the World Health Organization (WHO) included molecular markers (genotypic expression) as important predictive factors for diagnostic of brain tumors. In the brain, the expression and localization of water channel transporters, aquaporins (AQPs), are substantially modified in gliomas during tumorigenesis, cell migration, edema formation and resolution. Therefore, we hypothesized that molecular changes associated with AQP1 and AQP4 in the brain may be potential anticancer therapeutic targets. Our research group, for instance, has already shown a modulating action of triiodothyronine (T3), the biologically active form of thyroid hormone, on the AQP4 expression in the developing nervous system and on GBM cells. To test it, a bioinformatic analysis from publicly available data from international consortia was carried out. Here, we used RNA-seq as an experimental strategy and identified the differential expression of AQP1 and AQP4 transcripts numbers in gliomas tissue if compared to normal brain tissues. Indeed,  the AQPs genes were overexpressed in glioma patients. Among gliomas subtypes, AQP1 and AQP4 were overexpressed in astrocytoma (low grade-glioma) and classical (high grade-glioma). The overall survival analysis demonstrated both AQP genes can be used as a prognostic factor for patients with low grade-glioma, confirming the results of previous studies and reinforcing their clinical value. We also observed a correlation between the expression of genes involved in tyrosine and thyroid hormone pathways and AQPs. Namely: PNMT, ALDH1A3, AOC2, HGDATP1B1, ADCY5, PLCB4, ITPR1, ATP1A3, LRP2, HDAC1, MED24, MTOR and ACTB1 (Spearman’s coefficient = ≥ 0.20 and p-value = ≤ 0.05). These molecular pathways and AQP1 and AQP4 genes may be used to study new anti-tumor drugs and the molecular diagnosis of gliomas because showing potential as diagnostic and therapeutic biomarkers for malignant gliomas.

BANKING MEMBERS:
Presidente - 1121304 - ADRIANA XIMENES DA SILVA
Interno - 1298235 - EMILIANO DE OLIVEIRA BARRETO
Externa à Instituição - MYKAELLA ANDRADE DE ARAUJO - NENHUMA