Banca de DEFESA: DENISE MACEDO DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : DENISE MACEDO DA SILVA
DATE: 01/12/2022
TIME: 14:00
LOCAL: Virtual
TITLE:

ASSOCIATION OF POLYMORPHISM OF THE TLR4 896 A>G (rs4986790) AND iNOS -1173 C>T (rs9282799) GENES WITH CERVICAL CANCER IN HPV-INFECTED WOMEN IN A SAMPLE OF THE POPULATION OF ALAGOAS, BRAZIL: A CASE-CONTROL AND META-ANALYSIS STUDY 

KEY WORDS:

Polymorphism. Cervical cancer. HPV. Toll-Like Receptor 4.Nitric Oxide SynthaseType II. Alagoas. 

PAGES: 135
BIG AREA: Ciências da Saúde
AREA: Enfermagem
SUMMARY:

Cervical canceris one of the leading causes of death among women in the world, representing the fourth most frequent and fourth leading cause ofcancer death in the female population worldwide.This malignant neoplasm mainly affects socioeconomically vulnerable women 

and approximately 90% of cases are associated with high-risk HPV infection.In addition, immunological and genetic factors such as Single NucleotidePolymorphisms (SNPs) present in host genes may play animportant role in virus clearanceor cervical cancer susceptibility, such as SNPs in Toll-Like Receptor 4 genes (TLR4) and Inducible Nitric Oxide Synthase (iNOS).Thus, the present study aimed to evaluatethe association of TRL4 896 A>G (rs4986790) and iNOS -1173 C>T (rs9282799) gene polymorphisms with cervical cancer in women infected with HPV in a sample of the populationof Alagoas, Brazil.Thisis a case-control study, where 99 samples were genotyped for the TRL4 896 A>G gene (72 healthycontrols, 27 cases of HPV+ cervical cancer) and 120 samples for iNOS -1173 C>T (82 healthy controls, 38 cases of HPV+ cervical cancer) using the real-time Polymerase Chain Reaction (PCR) technique.Additionally, a systematic review and meta-analysis of the TLR4 896 A>G (rs4986790) polymorphism was performed.In the case-controlstudy, the results referring to SNP 896A>G in the TLR4 gene showed a statistically significant difference when comparing allele and genotypic frequencies, wherethe 896G allele (p=0.01; OR with 95% CI=5.87 [1.41-24.40]) and 896A/G genotype were associated with increased risk of cervical cancer (p=0.01; OR with 95% CI=6.57 [1.51-28.56]).This demonstrates tha twhen comparingthe 896A/A homozygotewiththe 896A/G heterozygote the presence of a G allele was sufficient to increase the risk.The sampling power was 100% and the population was in agreement with the Hardy-Weinberg equilibrium (p=0.63).The resultsof the meta-analysisof the TLR4 896 A>G (rs4986790) polymorphism did not demonstrate significant associations, differing from the results found in thepopulation of Alagoas.This fact can be explained by the scarcity of association studies between the SNP TLR4 896 A>G (rs4986790) and cervical cancer, as well as thelow sample sizeofthestudiesincluded in the meta-analysis.In the overall analysis of the association between the polymorphic variant -1173C>T of iNOS and cervical cancer, no association was found.The rare genotype -1173T/T was notidentified in any groupand the heterozygote -1173T/C had a low frequency in this population.The distribution of genotypic frequencies in the populationwas in accordance with the Hardy-Weinberg equilibrium (p=0.88).It is concluded that the G allele in the SNP TLR4 896 A>G (rs4986790) represents a risk factor for cervical cancer in the population of Alagoas.In the meta-analysis with other populations, no associations were found.The iNOS -1173 C>T SNP wasnotassociatedwith cervical cancer in thispopulation.However, the results regarding the polymorphismof the iNOS -1173 C>T gene are inconclusive due to the limited sample size, which presented a low sampling power.Studies with a larger sample number can elucidate the influenceof this polymorphism on the cervical carcinogenesis process, since the number of current studies is limited.  

BANKING MEMBERS:
Interno(a) - 1867374 - ELAINE VIRGINIA MARTINS DE SOUZA FIGUEIREDO
Externo(a) ao Programa - 1046888 - MARCIO BEZERRA SANTOS
Externo(a) à Instituição - ANA CAROLINE MELO DOS SANTOS