Banca de DEFESA: MAISA DE ARAÚJO COSTA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : MAISA DE ARAÚJO COSTA
DATE: 28/04/2023
TIME: 14:00
LOCAL: ICBS - Sala 19
TITLE:

N-formyl-methion yl-leucyl-phenylalanine plays a neuroprotective and anticonvulsant role in the Status Epilepticus model

KEY WORDS:

N-formyl peptide receptor, N-formyl-methionyl-leucyl- phenylalanine, status epilepticus, hippocampus, brain, epilepsy, neurodegeneration, neuronal death.

PAGES: 50
BIG AREA: Ciências Biológicas
AREA: Fisiologia
SUBÁREA: Fisiologia de Órgãos e Sistemas
SPECIALTY: Neurofisiologia
SUMMARY:

Status epilepticus (SE) is described as continuous and self-sustaining seizures, which trigger hippocampal neurodegeneration, inflammation, and gliosis. N- formyl peptide receptor (FPR) has been associated with inflammatory processes. N-formyl-methionyl-leucyl-phenylalanine (fMLP) peptide plays an anti-inflammatory role, mediated by activation of G-protein-coupled FPR. Here, we evaluated the influence of fMLP peptides on the behavior of limbic seizures, memory consolidation, and hippocampal neurodegeneration process. Male Wistar rats (Rattus norvegicus) received microinjections of pilocarpine in the hippocampus (H-PILO, 1.2mg/μL, 1μL) followed by fMLP (1mg/mL, 1μL) or vehicle (VEH, saline 0.9%, 1μL). During the 90 minutes of SE, epileptic seizures
were analyzed according to Racine’s Scale. After 24 hours of SE, memory impairment was assessed by the inhibitory avoidance test and the neurodegeneration process was evaluated in hippocampal areas. There was no
change in latency and number of wet dog shakes (WDS) after administration of fMLP. Our results showed that the intrahippocampal infusion of fMLP was able to reduce the severity of seizures, as well as the number of limbic seizures. In addition, fMLP infusion protected memory dysfunction followed by SE. Finally, the intrahippocampal administration of fMLP attenuated the process of neurodegeneration in both hippocampi. Taken together our data suggest a new insight into the functional role of fMLP peptides, with important implications for their potential use as a therapeutic agent for the treatment of brain disorders, such as epilepsy.

BANKING MEMBERS:
Presidente - 1974414 - OLAGIDE WAGNER DE CASTRO
Interno(a) - 586.597.658-34 - CLAUDIO TORRES DE MIRANDA - UFAL
Interno(a) - 2033893 - MARCELO DUZZIONI
Externo(a) ao Programa - 1547952 - EURICA ADELIA NOGUEIRA RIBEIRO
Externo(a) à Instituição - EUCLIDES MAURÍCIO TRINDADE FILHO - CESMAC