Banca de DEFESA: LAURA MAÍSA SOUZA DOS SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LAURA MAÍSA SOUZA DOS SANTOS
DATE: 12/03/2021
TIME: 09:00
LOCAL: Instituto de Física (remotamente)
TITLE:

Biocompatible Photoresinsfor Microfabricationof Three-Dimensional Cell Scarffolds

KEY WORDS:

Biocompatible photoresin. Nanoparticles. cellular scaffolds. Atomic Force Microscopy. Two-Photon Polymerization.

PAGES: 118
BIG AREA: Ciências Exatas e da Terra
AREA: Física
SUMMARY:

The additive technique by Two-Photon Polymerization (P2F) has become an excellent microfabrication method among the many existing processes, as it allows the construction of three-dimensional microstructures with high spatial submicrometer resolution, with high geometric complexity and minimal use of photosensitive material. This powerful tool has attracted the interest of several areas of the science, including the area of tissue engineering with application in the manufacture of three-dimensional scaffold for cell culture. These microenvironments are designed to mimic environments in vivo and express their most important characteristics, enabling the study of the cellular behavior and the treatment of diseases with drug testing. Photosensitive resins are the raw material for the construction of scaffolds and, although there is a wide availability of these materials for biological studies, there is a great concern in making photoresist compounds biocompatible and with reduced cytotoxicity. In this work, it was demonstrated by the trypan blue exclusion method that the photoresist synthesized based on PentaerythritolTriacrylate (PETA) and Irgacure 369 with incorporation of semiconductor nanoparticles of CdSe/CdS and titanium dioxide (TiO₂) are biocompatible. In special, the sample with TiO₂ showed low cytotoxicity and high rate of cell proliferation that remained higher than the control group for 48 h. The biomechanical properties of human osteoblasts of the MG-63 strain in direct contact with the surface of the film made of photosensitive resin and groove-type scaffolds, in different geometric designs, were analyzed by an atomic force microscope. Osteoblasts grown on circular microgroove scaffolds exhibited significantly higher Young’s modulus compared to cells sown on the film, a result that was attributed to the reorganization of the cytoskeleton influenced by the geometry and curved topography of the cellular microenvironment. It is important to note that, as it is an innovative and pioneering line of research at the Federal University of Alagoas (UFAL), this is the first dissertation developed on 3D microfabrication via Two Photon Polymerization in the Graduate Program in Physics (PPGF) at UFAL. Therefore, this dissertation was elaborated with several relevant details of the technique, so that the results and studies discussed here can collaborate for the continuity and consolidation of this very challenging and incredible line of research at PPGF.

BANKING MEMBERS:
Presidente - 2181152 - EDUARDO JORGE DA SILVA FONSECA
Externo ao Programa - 1298235 - EMILIANO DE OLIVEIRA BARRETO
Interno - 1282899 - GLAUBER JOSE FERREIRA TOMAZ DA SILVA