Banca de QUALIFICAÇÃO: FLAVIA ADAIS ROCHA DOS SANTOS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : FLAVIA ADAIS ROCHA DOS SANTOS
DATE: 11/03/2021
TIME: 14:00
LOCAL: Google Meet
TITLE:

Revisiting Passiflora: new activities and uses for a highly relevant genre

KEY WORDS:

Antioxidant, Antiglicante, Passion fruit, Seed.

PAGES: 60
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Orgânica
SPECIALTY: Química dos Produtos Naturais
SUMMARY:

The residues generated by fruit growing, such as seeds, are rich in compounds of high nutritional and therapeutic value. Among them, we highlight the antioxidant compounds, responsible for neutralizing reactive species of oxygen and nitrogen produced by cells and that can cause damage to biomolecules. On the other hand, nucleophilic groups of amino acids, lipids and DNA can react with carbonyl groups of reducing sugars and generate advanced glycation products, also called AGEs, which are associated with the development of several diseases, such as diabetes and Alzheimer's. Therefore, the search for antioxidant and anti-glycolic compounds is relevant. Thus, the present work aims to investigate the antioxidant potential against species of biological importance, as well as anti-gluing of ethanolic extracts of passion fruit seeds, in different stages of maturation and of different species, aiming at their standardization, adding economic value to the by-product of the industries of fruit pulps. For this, samples of organic passion fruit seed, obtained in the fruit pulp industry located in the municipality of Marechal Deodoro and other sources, were subjected to extraction by Soxhlet with hexane, followed by extraction with ethanol. The ethanolic extract of the seeds (EESM) was characterized using high performance liquid chromatography with a diode array detector coupled to the mass spectrometer. The EESM was subjected to the determination of the total phenol content by the Folin-Ciocaulteu method; to the evaluation of the antioxidant activity through the uptake of hypochlorous acid, to the evaluation of the antioxidant activity by uptake of the radical superoxide anion, to the evaluation of the oxidative burst in activated neutrophils, to the evaluation of the lipid peroxidation through the use of lecithin unilamellar liposomes soybean and the evaluation of anti-glycant activity by quantifying free amino groups using fluorescamine. In addition, an electrochemical study was carried out to evaluate the application of passion fruit extract, as a redox mediator, by the presence of catecholic groups. Yields of 17.30% and 9.29% were obtained for hexane and ethanolic extracts, respectively. Ten compounds were identified in the EMES, namely: piceatanol, astringine, scirpusin B, scircpusin A and Isookanin-7-o-glycoside, naringenin-7-o-glycoside, tyrosine, phenylalanine and derivatives I and II of quadranguloside saponin. For the antioxidant tests, the MESE showed 322.1 ± 4.4 mg of EAG / g of dry extract, offered about 78% protection against lipid peroxidation for 30 min, presenting an IC50 of 1.68 ± 0.28 μg mL-1 in the hypochlorous acid assay, very close to that obtained for the positive control quercetin 0.33 ± 0.30 μg mL-1. For the evaluation of the uptake of the superoxide anion radical, the MESE showed about 40% inhibition for the concentration of 200 μg mL-1. The voltamograms obtained from the EESM after modifying the electrode with multi-walled carbon nanotubes (MWCNT) presented a reversible or almost reversible system, initiated with the oxidation of an electroactive species in the solution, with consequent increase in current. It was also possible to observe the electropolymerization of the electroactive species on the surface of the electrode modified with MWCNT. The results obtained so far reveal the antioxidant potential vis-à-vis biologically important and anti-glycant species acting in the intermediate stage of the reaction. There is also potential for the bioelectrochemical use of passion fruit extract.

BANKING MEMBERS:
Presidente - 2120103 - MARILIA OLIVEIRA FONSECA GOULART
Interno - 1613338 - JOSUE CARINHANHA CALDAS SANTOS
Interno - 1119939 - ANTONIO EUZEBIO GOULART SANTANA
Externa ao Programa - 1006306 - JADRIANE DE ALMEIDA XAVIER
Externa à Instituição - IARA BARROS DE OLIVEIRA - IFAL