Banca de QUALIFICAÇÃO: TAMARA TAIS DOS SANTOS

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : TAMARA TAIS DOS SANTOS
DATE: 26/04/2024
TIME: 08:00
LOCAL: Google meet
TITLE:

STUDY OF VOLATILE ORGANIC COMPOUNDS OF MURRAYA PANICULATA (L. Jack) INVOLVED IN THE ATTRACTION OF CITRI DIAPORIN (KUWAYAMA, 1908)

KEY WORDS:

 Psyllid; Huanglongbing; Citrus; Nonanal

PAGES: 50
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUMMARY:

Citrus farming is an important agricultural activity for Brazil, which is among the five largest citrus producers in the world, together with China, the European Union (EU), the United States and Mexico (USDA, 2022; ETENE,2022) . Among the various pests that affect this crop, the psyllid Diaphorina citri Kuwayama (Hemiptera: Psyllidae) stands out; insect that sucks phloem sap and is a vector of the bacterium Candidatus liberibacter that causes the citrus disease known as Huanglongbing (HLB) (Grafton-Cardwell et al., 2013). Diaphorina citri has a variety of hosts belonging to the genera Citrus and Murraya, Murraya paniculata (L.) Jacq is the preferred host. Our objective with this study is to identify the volatile organic compounds involved in the attraction of D. citri to the host plant belonging to the Rutaceae family. To obtain the volatile constituents of the leaves and flowers of Murraya paniculata, the dynamic headspace technique was used, for which aeration systems were set up using polyester plastic bags (Wyda®). The volatile organic compounds produced were collected by placing the plant inside a previously cleaned plastic bag. Pure air was introduced into this environment and removed with vacuum pumps, passing through a glass column containing an adsorbent polymer (Porapack, 50 mg, Supelco, USA) at the end of the system to adsorb volatile organic compounds. The aeration time was 8 hours during the day, and adsorbed organic compounds were removed by passing 500 µL of HPLC-grade hexene (Merck, Darmstadt, Germany). To obtain the essential oil, the steam distillation method was used. The extracts obtained were stored in a freezer at -80 °C for subsequent analysis. Extracts were analyzed by GC/FID and GC/MS. Compound identification was performed by analyzing the mass spectra of the compounds and comparing them with the data listed in the NIST GC-MS library and the Kovats retention index (KI). Among the compounds, it was possible to identifyCaryophyllene as the major compound for leaf extracts and leaf oil, while nonanal and linalool were the major compounds for flowers. Work continues to generate active compounds and attractive baits for field testing

COMMITTEE MEMBERS:
Presidente - 1119939 - ANTONIO EUZEBIO GOULART SANTANA
Externo(a) à Instituição - ARIANE MORGANA LEAL SOARES
Externo(a) à Instituição - JOÃO GOMES DA COSTA
Interno(a) - 1294906 - RUTH RUFINO DO NASCIMENTO