Banca de DEFESA: ELVIS MAX XAVIER DE LIMA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ELVIS MAX XAVIER DE LIMA
DATE: 14/09/2021
TIME: 09:30
LOCAL: Google Meet
TITLE:

Development of Paper-Based Analytical Device (µPAD) for Colorimetric Detection and Quantification of L-Tyrosine

KEY WORDS:

Colorimetry; µPAD; L-Tyrosine; Food supplements;

PAGES: 52
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUMMARY:

L-Tyrosine is an essential amino acid, a precursor for the formation of neurotransmitters such as dopamine and norepinephrine. Its intake is essential for the maintenance of these neurotransmitters and the reduction of muscle fatigue and stress. So, several companies have been developing food supplements to assist in the L-Tyrosine diet. Currently, the quality control of this type of supplement is done through robust techniques such as HPLC and this ends up making the analysis process more expensive and requiring specialized labor. To achieve this process cheaper and easier to perform, this work aims the development of a low-cost paper-based microfluidic device (µPAD) that can perform the determination of L-Tyrosine in samples of dietary supplements. The µPADs were coupled to fluorescence and colorimetric detection. It was observed that the system has better analytical signal at pH = 12. Among the three types of layout covered in this work, the one with circular detection zones and 2 mm thick hydrophobic barriers around them obtained the best response. The influence of the RGB color channels was also studied in this work and it was observed that for the system that used fluorescence as detection technique, channel B performed better than the other channels while by colorimetry channel G had the best analytical signal.

BANKING MEMBERS:
Externo à Instituição - BRUNO GABRIEL LUCCA - UFMS
Presidente - 1127609 - DIOGENES MENESES DOS SANTOS
Interna - 426640 - FABIANE CAXICO DE ABREU GALDINO