Banca de QUALIFICAÇÃO: KEILANE CRUZ FRANCA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : KEILANE CRUZ FRANCA
DATE: 01/10/2021
TIME: 14:00
LOCAL: Google Meet
TITLE:

Evaluation of insecticidal activity of Genipa americana bark on the fruit borer Cerconota anonella

KEY WORDS:

Fruit borer; lectin; integrated management.

PAGES: 40
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUMMARY:

The fruit borer, Cerconota anonella (Sepp., 1830), is a species belonging to the order Lepidoptera: Oecophoridae, and
considered one of the most serious pests of soursop, of the genus Annona. The significant damages caused to the
fruits are seen by the rotting of the pulp, in addition the attacked part is hardened and blackened, which reduces its
commercial value. In order to control this pest, methods such as bagging the unripe fruits using perforated bags are
used, but it is impracticable due to the high demand for labor. In addition, insecticides are used, however the high
toxicity of the compounds used on the environment and non-target organisms have led to an increase in the search for
natural products free of toxicity and effective in controlling insect pests. Lectins - proteins or glycoproteins that recognize
carbohydrates and bind cells - isolated from plants have shown insecticidal activity and for this reason have been
extensively studied. The jenipapeiro (Genipa americana L.), belonging to the family Rubiaceae, originates from Central
America and is currently distributed in the tropical regions of several countries in America, Asia and Africa. There are
no records that this plant species is a target of Cerconota anonella, the present study aims to evaluate the insecticidal
activity of the lectin extracted from Genipa americana bark against the fruit borer. Therefore, it is intended to isolate and
purify a lectin from the jenipapeiro bark, firstly preparing the crude extract in Tris-HCl 50 mM, pH 8.0, from the bark of
the plant, followed by saline precipitation with ammonium sulfate. Subsequently, the fraction with the highest lectin
activity will be subjected to liquid chromatography in a gel filtration column, followed by electrophoresis (SDS-PAGE).
Once isolated, the insecticidal activity of the lectin against Cerconota anonella will be evaluated in behavioral bioassays
under laboratory conditions.

BANKING MEMBERS:
Presidente - 2089586 - FRANCIS SOARES GOMES
Interno - 1811274 - HUGO JUAREZ VIEIRA PEREIRA
Interna - 1294906 - RUTH RUFINO DO NASCIMENTO