Development of electrochemical sensors for electroanalysis of homocysteine (HCys) via platforms using nanostructured electrodes and electrodes modified with molecular imprinted polymers.
Molecularly imprinted polymers, chemically modified electrodes, homocysteine.
The determination of the homocysteine (HCys) levels into the cellular and intracellular tissues is an important diagnosis
technique, which can predict health risks by identifying illnesses like leukemia, Alzheimer, and cardiac and metabolic
problems. In this context, the enhancement of more selective and sensible analytical methods has a big relevance for
the detection of this chemical. Given this, our work aims the development of electroanalytical strategies whose are
based on different platforms for the separation and quantification of HCys, where we want to build an approach based
on magnetic molecular imprinted polymers (mag-MIP), as well nanostructured platforms for the determination of HCys
via electrochemical techniques. The features of the selected materials were described by Fourier transformed infrared
spectroscopy, field-emission scanning electron microscopy and, vibrating sample magnetometry for the mag-MIP,
which demonstrated good indications on their composition, morphology, and chemical and physical properties. Other
than that, we aim to use cyclic voltammetry to investigate the homocysteine electrochemical behavior on the modified
electrodes with MIPs and on the nanostructured electrodes by using real samples. This way, the work accomplished so
far shows the development of well succeeded methodologies into the initial steps of the electrochemical sensors, relevant to medical and clinical analysis when it comes to separation and quantification of HCys.