Banca de DEFESA: KEILANE CRUZ FRANCA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : KEILANE CRUZ FRANCA
DATE: 01/04/2022
TIME: 14:00
LOCAL: Google Meet
TITLE:

Evaluation of the effect of extract, protein fraction and lectin from Genipa americana bark on the fruit
borer of Cerconota anonella (Lepidoptera: Depressaridae)

KEY WORDS:

Annona; fruit borer; natural insecticides; lectin; integrated management.

PAGES: 80
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUMMARY:

The fruit borer, Cerconota anonella (Sepp., 1830), is a species belonging to the order Lepidoptera: Depressaridae,
and considered one of the most serious pests of plants from genus Annona. The significant damages caused to the
fruits are seen by the rotting of the pulp, in addition the attacked part is hardened and blackened, which reduces its
commercial value. In order to control this pest, methods such as bagging the unripe fruits using perforated bags are
used, but it is impracticable due to the high demand for labor. In addition, insecticides are used, however the high
toxicity of the compounds used on the environment and non-target organisms have led to an increase in the search 

for natural products free of toxicity and effective in controlling insect pests. Lectins - proteins or glycoproteins that
recognize carbohydrates and bind cells - isolated from plants have shown insecticidal activity and for this reason have
been extensively studied. The jenipapeiro (Genipa americana L.), belonging to the family Rubiaceae, originates from
Central America and is currently distributed in the tropical regions of several countries in America, Asia and Africa.
There are no records that this plant species is a target of Cerconota anonella, the present study aims to evaluate the
insecticidal activity of the extract, protein fraction and lectin extracted from Genipa americana bark against the fruit
borer. Therefore, it is intended to isolate and purify a lectin from the jenipapeiro bark, firstly preparing the crude
extract in Tris-HCl 50 mM, pH 8.0, from the bark of the plant, followed by saline precipitation with ammonium sulfate.
Subsequently, the fraction with the highest lectin activity will be subjected to liquid chromatography in a gel filtration
column. Once isolated, the insecticidal activity of the lectin against Cerconota anonella will be evaluated in behavioral
bioassays under laboratory conditions.

BANKING MEMBERS:
Presidente - 2089586 - FRANCIS SOARES GOMES
Interno - 1811274 - HUGO JUAREZ VIEIRA PEREIRA
Externa à Instituição - JANAÍNA KÍVIA ALVES LIMA
Externo ao Programa - 1653558 - LUCIANO APARECIDO MEIRELES GRILLO