Banca de DEFESA: FLAVIA ADAIS ROCHA DOS SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : FLAVIA ADAIS ROCHA DOS SANTOS
DATE: 28/03/2022
TIME: 14:00
LOCAL: Google Meet
TITLE:

Antioxidant, antiglycant and antidiabetic potential of ethanolic extract from seeds of
Passiflora and picetanol in vitro

KEY WORDS:

Antioxidant, Antiglicant, Passion Fruit, Seed. waste

PAGES: 60
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Orgânica
SPECIALTY: Química dos Produtos Naturais
SUMMARY:

The residues generated by fruit growing, such as seeds, are rich in compounds of high nutritional value and
therapeutic. This study investigated the antioxidant, antidiabetic and antiglycant potential in vitro of extracts
ethanol content from seeds of Passiflora edulis from Alagoas (EPED1), P. edulis from Bahia (EPED2) and P. cincinnata
(EPCIN), an important by-product of the juice industry, along with one of its chemical constituents, the
piceatannol (PIC). Ten compounds were identified in the EESM, namely: piceatanol, astringin, scirpusin B,
scircpusin A and Isookanine-7-o-glycoside, naringenin-7-o-glycoside, tyrosine, phenylalanine and derivatives I and II of
of quadranguloside saponin. The extract IC50 values for the DPPH• and HOCl sequestration assays
showed the high antioxidant potential of all extracts and PIC when compared to quercetin (QCT).
Among all extracts, EPED1 exhibited the highest activity for α-amylase (IC50 of 32.1 ± 2.7 µg mL-1) and inhibition of
human recombinant enzyme DPP-4 (IC50 of 71.1 ± 2.6 µg mL-1), positive controls were acarbose (ACB, 0.40
± 0.05 µg mL-1) and sitagliptin (STG, 0.005 ± 0.001 µg mL-1), respectively. PIC stood out for inhibition of α-
glucosidase with IC50 of 76.0 ± 1.9 µg mL-1, the positive control, ACB had 251.6 ± 4.5 µg mL-1. The extracts and PIC
inhibited the formation of advanced glycation end products (AGE) in a dose-dependent manner through the method
of bovine serum albumin (BSA) glycation by reducing sugars or methylglyoxal (MGO). Furthermore, the training
of β-amyloid fibrils decreased as the sample concentration increased, reaching 100% inhibition
greater than 300 µg mL-1, except for EPCIN. This suggests that EPED1, EPED2 and PIC can prevent glycation. O
cell viability assay showed that EPED1 had no effect on normal bronchial epithelial cells (BEAS-2B)
and alpha mouse liver (AML-12) up to 100 and 50 µg mL-1, respectively. Lower extract concentrations
(10 µg mL-1) were cytotoxic to non-malignant mammary epithelial cells (MCF-10A). It was found that EPED1 and
PIC are able to protect cultured human cells from oxidative stress caused by the carcinogen NNKOAc
at 100 µM. This investigation collected some in vitro evidence of the potential use of P. edulis seeds as
an alternative for the treatment of diabetes, although more research is still needed.

BANKING MEMBERS:
Interno - 1119939 - ANTONIO EUZEBIO GOULART SANTANA
Externa à Instituição - IARA BARROS VALENTIM - IFAL
Interna - 1006306 - JADRIANE DE ALMEIDA XAVIER DOS SANTOS
Interno - 1613338 - JOSUE CARINHANHA CALDAS SANTOS
Presidente - 2120103 - MARILIA OLIVEIRA FONSECA GOULART