Banca de QUALIFICAÇÃO: MARTA MARIA OLIVEIRA DOS SANTOS

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : MARTA MARIA OLIVEIRA DOS SANTOS
DATE: 31/08/2022
TIME: 14:00
LOCAL: Sala de reuniões do IQB
TITLE:

Production of fermentable sugars for second generation ethanol generation using endoglucanases from yeasts and filamentous fungi.

KEY WORDS:

Enzymes, Box-Benhken, Purification, Biotechnological applications.

PAGES: 91
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUMMARY:

Currently, the world is facedwiththeprospectof a significantincrease in demand for ethanol. Thus, to avoidthelimitofsupplyorcompetition for the use ofland for energygeneration, it is necessary to invest in thedevelopmentofsecondgenerationtechnologies for theproductionofethanol. Thiscanoccurthroughlignocellulosicbiomass, material abundant in agro-industrialresidues. In search ofalternatives for production, the use ofbiomass for theproductionofenzymes (in solidstatefermentation) wasidealizedandlatertheoptimizationofthecatalyticconditions to improvetheactivityoftheisolatedandcharacterizedenzymes. Thepresentstudyaimed to reporttheproductionofendoglucanasebythesolidstatefermentationtechniquebytheactionofthefilamentousfungusPenicilliumroqueforti ATCC 10110 usinglignoulosicresidues as substrate sugar canebagasse (BCA), coconuthusk (FC), pineapplecrown (CA) andwheatbran (FT). Thecharacterizationoftheenzymeproducedwascarried out in termsofthermostabilityandstabilityatdifferentpHsand its activityagainsttheadditionofmetalsandorganicsolventsand, finally, the application oftheenzymecontained in theenzymaticcrudeextractproducedwas in thesaccharificationofsugarcanelignocellulosicresidues (BCA), coconuthusk (CC), wheatbran (FT), cocoafruithusk(CFC) andcocoaseedhusk (CSC), applyingscanningelectronmicroscopytechnique to confirmthesaccharificationefficiency. TheBox-Behnken experimental design wasalsoapplied to optimizefactorsthatinfluencethesaccharificationprocess, namely, substrate (SB), incubation time (T) andenzymeconcentration (EC). Themodelfoundpresented as optimalconditionsat6hours, andmaximumlevelsof SB (15%) and EC (440 U/mL), andthe experimental responseobtainedwas 253.19 mg/g reducing sugar. Thisstudy shows thatthecrudeenzymaticextractofPenicilliumroqueforti ATCC 10110 containingendoglucanasecanbeapplied in industries.

BANKING MEMBERS:
Externo à Instituição - NADABE DOS SANTOS REIS
Interno - 2089586 - FRANCIS SOARES GOMES
Presidente - 1811274 - HUGO JUAREZ VIEIRA PEREIRA
Externo à Instituição - MARCELO FRANCO - UESC-BA
Externa à Instituição - SONIA SALGUEIRO MACHADO - UFAL