Development of a colorimetric and fluorimetric kit to identify acid phosphatase in fluids from sexual crimes
Crimes against women; sexual crimes; diagnostic kit; semen; colorimetric molecular markers.
In recent years, sexual crimes against women have increased exponentially, with more than 250,000 cases since 2017. In the preliminary analysis of biological fluids found at the crime scene, rustic methods are still used to identify the presence of semen, which consumes a greater amount of time to solve these crimes. In order to meet this demand, this project aims to develop a colorimetric kit for the identification of semen in biological fluids. This project will be carried out in collaboration with the State University of Maringá, the Official Forensics Department of Alagoas and the Federal Police of Pernambuco, enabling the integration between the university and other public bodies. Colorimetric probes applied to paper-based analytical devices will be synthesized in order to indicate the presence of semen through an indicator system, developed from the reaction with the acid phosphatase enzyme in forensic biological samples from sexual crimes, allowing for qualitative analysis. and semiquantitative acid phosphatase. The innovations achieved in this project involve the development of a new method for semen detection, from a portable and simple device, allowing the achievement of reliable, fast, selective and safe results, which will serve as additional evidence for the elucidation of crimes of a sexual nature against women, children and adolescents.