Banca de DEFESA: CLEDSON BARROS DE SOUZA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : CLEDSON BARROS DE SOUZA
DATE: 29/11/2022
TIME: 15:00
LOCAL: Google Meet
TITLE:

Production, parcial isolation and characterization of laccases, caseinases e collagenases from Amphobotrys ricini
obtained by solid state fermentation.

KEY WORDS:

Amphobotrys ricini; Laccase; Discoloration; Caseinase; Collagenase

PAGES: 112
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUMMARY:

Amphobotrys ricini is a phytopathogenic fungus that infects the plant species known as castor bean. It is known that the fungus produces enzymes of biotechnological importance to colonize plants. Given the little knowledge about their secretion products, it is important to investigate their ability to produce proteases and ligninases. Objectives: To produce, partially isolate and characterize proteases and ligninases produced by A. ricini through solid-state cultures and propose applications for the enzymes obtained. Methodology: The fungus was cultivated on different agro-industrial residues and co-products, to define the best conditions for the production of proteases and ligninases, and from them, the enzymes were extracted and subjected to isolation techniques. The ligninases obtained had their dye discoloration potential measured and the proteases were subjected to activity tests on casein, collagen and milk coagulation. Results and Discussion: Among investigated ligninases, only laccases were found. The fungus was able to promote discoloration in microbiological medium containing bromophenol blue. Laccases were partially isolated by salt precipitation and ion exchange chromatography. Among the activities presented by the proteases, it was possible to confirm activity on casein, collagen and the ability to clot milk. The enzymes were partially purified by extraction in a biphasic aqueous system. Conclusions: The fungus is a producer of laccases capable of bleaching dyes, its caseinases can be applied in the manufacture of dairy products and the presence of collagenolytic activity was confirmed, which is of great importance in the food and pharmaceutical industry.

BANKING MEMBERS:
Presidente - 1811274 - HUGO JUAREZ VIEIRA PEREIRA
Interno(a) - 2089586 - FRANCIS SOARES GOMES
Externo(a) ao Programa - 036.711.126-89 - JOSE MARIA RODRIGUES DA LUZ - UFAL
Externo(a) ao Programa - 1653558 - LUCIANO APARECIDO MEIRELES GRILLO
Externo(a) ao Programa - 2024367 - MELISSA FONTES LANDELL
Externo(a) ao Programa - 1653165 - VALTER ALVINO DA SILVA