Banca de DEFESA: MARCONE GOMES DOS SANTOS ALCANTARA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARCONE GOMES DOS SANTOS ALCANTARA
DATE: 01/03/2023
TIME: 09:30
LOCAL: SALA DE PÓS-GRADUAÇÃO - PPGQB
TITLE:

Development of a colorimetric and fluorimetric kit to identify acid phosphatase in fluids from sexual crimes

KEY WORDS:

Crimes against women; sexual crimes; diagnostic kit; semen; colorimetric molecular markers.

PAGES: 70
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUMMARY:

In recent years, sexual crimes against women have been increasing exponentially, with more than 250,000 cases since 2017. The search for biological fluids at the crime scene can be crucial in the preliminary analysis, however, the methods currently available demonstrate it if impractical and outdated, which consumes time until the solution of these crimes. In order to change this reality, this project aims to develop a colorimetric and fluorescent kit for identifying semen in biological fluids, based on a phosphorylation reaction using the agent diethyl chlorophosphate followed by the dealkylation of the ethyl groups using the agent Iodine-TMSi, aims at the synthesis of colorimetric and fluorescent probes derived from Resorufin and azo dyes. The detection mechanism will be based on the enzymatic reaction of acid phosphatase with the probes, resulting in a color change and fluorescence emission when in contact with forensic biological samples. The synthetic route used was effective for the synthesis of the proposed probes, with variable yields from 60% to 65%. The first synthesized probe, derived from the resorufin dye, showed promising results in preliminary tests with the acid phosphatase enzyme, acting in a colorimetric way and emitting fluorescence at a wavelength of 580 nm at pH 8, acting through an off-on mechanism, the probe 3 was also able to identify real semen samples through a colorimetric change. The innovations achieved in this project involve the development of a new method for detecting semen, using a portable and simple-to-use device, allowing reliable, fast, selective and safe results to be obtained, which will serve as additional evidence for the elucidation of crimes of a sexual nature against women, children and adolescents.

BANKING MEMBERS:
Interno(a) - 1997465 - DANIELA SANTOS ANUNCIACAO
Interno(a) - 2089941 - DIMAS JOSE DA PAZ LIMA
Interno(a) - 1006306 - JADRIANE DE ALMEIDA XAVIER
Presidente - 1613338 - JOSUE CARINHANHA CALDAS SANTOS
Externo(a) à Instituição - MAREK HENRYQUE FERREIRA EKERT