Banca de DEFESA: TAUANE DOS SANTOS ROCHA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : TAUANE DOS SANTOS ROCHA
DATE: 25/07/2023
TIME: 10:00
LOCAL: SALA DE PÓS-GRADUAÇÃO - PPGQB
TITLE:

PROTEIN AGGREGATION AS A CONSEQUENCE OF NON-ENZYMATIC GLYCATION: THERAPEUTIC INTERVENTION THROUGH THE USE OF PHENOLIC COMPOUNDS

KEY WORDS:

piceatannol; oxidative stress; carbonyl stress; glycation.

PAGES: 75
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUMMARY:

Glycation, also known as the Maillard reaction, is a non-enzymatic reaction that occurs in the biological environment or in foods between sugars and nucleophilic groups of biomolecules. One of the consequences of glycation is the formation of AGEs that can permanently alter the structure and function of proteins through post-translational modifications that can lead to misfolding, accumulation and finally the formation of amyloid aggregates. These aggregates, in turn, are related to neurodegenerative diseases including Alzheimer's, Prion and Parkinson's. the percentage of glycated blood used to assess the average serum glucose concentration over the last 3 to 4 months. Hemoglobin is a heme protein whose main function is to provide oxygen to various body tissues, glycation, however, can compromise its main function. The objective of this study was to evaluate the progression of hemoglobin (Hb) glycation through the formation of advanced glycation end products (AGEs), structural changes in the Soret band, and the formation of Hb aggregates, from the interaction with the thioflavin T probe (ThT) and with the Congo red probe, considering using glucose and fructose as the reducing sugars in the glycation process of Hb. From these studies, it is possible to notice that such alterations occurred in such a way that it was possible to perceive that the system in which hemoglobin was glycated by fructose presented greater formation of AGEs, since fructose is a much more reactive reducing sugar than glucose. And in the system treated with piceathanol, it was possible to observe its protective effect on hemoglobin from alterations caused by reducing sugars in its structure, as well as an AGE inhibition capacity of 88.99% at a concentration of 300 µM. The present study aims to evaluate a pure phenolic compound as an inhibitor of hemoglobin glycation and maintenance of its native structure.

BANKING MEMBERS:
Externo(a) à Instituição - IARA BARROS VALENTIM
Interno(a) - 1006306 - JADRIANE DE ALMEIDA XAVIER
Interno(a) - 1613338 - JOSUE CARINHANHA CALDAS SANTOS
Interno(a) - 2343745 - JULIO COSME SANTOS DA SILVA
Presidente - 2120103 - MARILIA OLIVEIRA FONSECA GOULART