Banca de QUALIFICAÇÃO: MAIARA INGRID CAVALCANTE QUEIROZ

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : MAIARA INGRID CAVALCANTE QUEIROZ
DATE: 17/08/2023
TIME: 08:00
LOCAL: Sala de reuniões do IQB
TITLE:

Chronic exposure of fishermen to a polluted environment (Lagunar Mundaú-Manguába Complex - CELMM) leads to oxidative stress and functional changes in blood cells

KEY WORDS:

Environmental contamination; Mercury; Blood cells.

PAGES: 114
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUMMARY:

Mercury is a toxic metal that can cause environmental damage, as well as human exposure to these contaminants can lead to oxidative stress that leads to several comorbidities. In the state of Alagoas, Mundaú Lagoon (which is part of the Mundaú-Manguaba Estuarine Complex) is an important source of fish and molluscs for the population, but according to recent data from our group, the contamination of these metals has promoted damage to blood cells, with emphasis on Hg. Therefore, the aim of this work is to evaluate the redox state, functional and structural alterations of blood cells of people who live around the CELMM, comparing with people who do not have contact with such an environment. Another point evaluated was that in the literature it is known that cardiovascular diseases are major death inducers worldwide, however studies that assess whether metals, such as Hg, have an additive effect on such pathologies are scarce. Knowing that atherosclerosis is a disease caused by the deposition of cholesterol in the arteries and that it can lead to acute myocardial infarction, we used an animal model, genetically modified, that has atherosclerosis, the LDLr - / - mice. Therefore, in this second part of our work, we evaluated this model, treating the animals with mercury chloride (30 days). In the study with humans so far, we have evaluated blood cells (60 fishermen and 65 controls), where higher levels of Hg in whole blood were observed; in erythrocytes there is a decrease in oxygen binding capacity, structural alteration and in the redox system. Also, the lymphocytes showed an exacerbated production in the production of O2 •- and H2O2. Furthermore, regarding the second part of our study, in the LDLr - / - animals treated with HgCl2, the erythrocytes had a decrease in oxygen uptake; change in white blood cells, increase in phagocytic activity, accumulation of Hg in the organs, as well as alteration in kidney and liver function, and even greater release of cytokines. In isolated liver mitochondria, a decrease in respiration, loss of membrane potential and an increase in H2O2 generation were found. Finally, our results, so far, show that, regardless of the model used, Hg contamination leads to systemic damage in the organism.

BANKING MEMBERS:
Externo(a) ao Programa - 2151027 - ALEXANDRE URBAN BORBELY
Interno(a) - 2022362 - ANA CATARINA REZENDE LEITE
Interno(a) - 1869296 - CINTYA D'ANGELES DO ESPIRITO SANTO BARBOSA
Externo(a) à Instituição - HELENA COUTINHO FRANCO DE OLIVEIRA