Banca de DEFESA: MARIA THALILLIAN SANTOS FIGUEIREDO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARIA THALILLIAN SANTOS FIGUEIREDO
DATE: 17/04/2024
TIME: 14:00
LOCAL: Auditório do CRAD, Campus de Arapiraca, UFAL
TITLE:

Genomic annotation of the repetitive fraction of Syagrus coronata obtained through Genome Skimming assembly

KEY WORDS:

Ouricuri; repetitive DNA; genome lenght.

PAGES: 50
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitotecnia
SUMMARY:

Plant genomes vary considerably in size and composition, with repetitive DNA being the most crucial factor in diversity, accounting for up to 90% of the genome and, of which the repetitive DNA consists of satellite and transposable elements. Syagrus coronata is an endemic palm tree from Brazil belonging to the Arecaceae family. Its geographic distribution includes the Caatinga and Atlantic Forest regions. Currently, there are studies of chloroplast and mitochondrial genomes, as well as microsatellite markers of the nuclear genome. However, the dynamics and evolution of the nuclear genome, including genome size and repetitive DNA composition, are not well understood. This study aims to estimate the genome size and characterize repetitive sequences in S. coronata. The genome size was estimated using flow cytometry and k-mer approaches. Repetitive sequences were analyzed using paired-end reads through RepeatExplorer pipelines, and the species Syagrus weddelliana and Cocos nucifera were included for genomic comparison. The genome size was estimated to be 2,27±0,026 Gbp/1C using flow cytometry and estimates using k-mer showed 1.2 Gb. The repetitive analysis showed that repetitive sequences represent 58,43 % of S. coronata genome, being the LTR retrotransposons were most abundant (48.71 %). Among the LTR retrotransposons, the Angela was the most abundate, wich 25.66 %. Other repetitive elements such as transposons, ribosomal DNA, and satellite sequences exhibited lower levels of repetition. The satellites exhibited high diversity in number and monomer length; however, they made a minor contribution to the genome size. We conclude that S. coronata has a genome with moderately repetitive DNA sequences, with LTR retrotransposons being the most abundant.

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