Banca de DEFESA: MARIA APARECIDA DOS SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARIA APARECIDA DOS SANTOS
DATE: 16/09/2021
TIME: 09:00
LOCAL: Plataforma Online Google Meet
TITLE:

ASSEMBLY, ANNOTATION OF PLASTIDIAL AND MITOCHONDRIAL GENOMES OF Stylosanthes seabrana and Stylosanthes guianensis

KEY WORDS:

Allopolyploidy. Chloroplast. Organelle Genome. Mitochondria. Stylosanthes.

PAGES: 49
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitotecnia
SUMMARY:

The genus Stylosanthes Sw. is highly diverse and polymorphic, many species are present in regions of low rainfall, grow in calcium-poor soils, are able to fix nitrogen in the soil, and are of great importance in the formation of pastures and also in the recovery of degraded areas. Despite its economic importance, the genus Stylosanthes still presents little genetic characterization and its taxonomy and systematic complexity makes the delimitation and identification of species difficult. Organellar genomes, such as those of chloroplast and mitochondria, can be very useful in resolving phylogenetic relationships in groups with complex systematics. Due to their uniparental, mainly maternal inheritance in angiosperms. The objective of this work was to obtain and analyze comparatively the complete Plastome and Mitogenome of two species of the genus Stylosanthes: S. seabrana, S. guianensis in order to increase the knowledge about the evolutionary relationships and genetic improvement of the genus. The genomes of each species were assembled using NOVOPlasty v. 3.8.3 software, using as reference the plastidial and mitochondrial genome of Stylosanthes hamata. After assembly, with the help of Geneious v. 9.1.8 software, the genomes were analyzed and compared. The circular plastidial genome for Stylosanthes seabrana showed a total size of 156,499 bp, and a total of 125 genes were identified. In turn, the plastidial genome of Stylosanthes guianensis showed a total size of 156,785 bp, being identified a total of 127 genes. The mitochondrial circular genomes obtained for the two species presented a length of 468,896 bp in S. guianensis, being identified 55 genes and in S.seabrana of 523,870 bp, being identified 57 genes. The plastid and mitochondrial genomes of the species of the genus Stylosanthes analyzed in this study proved to be highly conserved and can be used for further phylogenetic studies and contribute to the genetic improvement programs of these species.

BANKING MEMBERS:
Presidente - 3073207 - ANDRE SECO MARQUES DA SILVA
Interno - 2380775 - CICERO CARLOS DE SOUZA ALMEIDA
Externo à Instituição - LUIS GUSTAVO RODRIGUES SOUZA - UFPE