Banca de DEFESA: ALEYRES BISPO CHAGAS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ALEYRES BISPO CHAGAS
DATE: 17/09/2021
TIME: 14:00
LOCAL: Plataforma Online: Google Meet
TITLE:

Study of biological activity in Enterolobium Contortisiliquum (Vell.) Morong seeds using the biospeckle technique

KEY WORDS:

Seeds. Tamboril. Scarification. Degraded areas restoration.

PAGES: 60
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitotecnia
SUMMARY:

The biospeckle laser technique is based on an optical interference phenomenon that happens when a coherent light beam falls on the material surface, where there is activity of biological origin. Thus, the objective of this work was to characterize the metabolic activity through the biospeckle technique, correlating it with the seed imbibition curve after chemical and mechanical scarification and at different times. For this, newly collected E. contortisiliquum seeds were subjected to five different treatments, namely Control, H2SO4(PA) at 5 minutes, H2SO4(PA) at 10 minutes, H2SO4(PA) at 15 minutes and Mechanical Scarification, and exposed at soak times of 0, 8, and 72 hours. The experiment was carried out in a completely randomized design and all treatments consisted of five replicates consisting of 100 seeds each. The seeds were weighed on an analytical balance with a precision of 0.001 g before and after imbibition and an imbibition curve was established. For the analysis of the metabolic activity of seeds by the biospeckle technique, newly collected E. contortisiliquum seeds were used and submitted to the same five treatments described above, and soaking time of 0, 4 and 72 hours, with five replicates of 57 seeds. The TCAPTURE, COMMAND PROMPT, STDM programs were used, and among the lines that form the Y axis of the matrix, the line 100 was established as the standard line for the study of changes occurring in a region of the sample surface. The analysis of the speckle patterns temporal evolution was performed using a THSP image, followed by the calculation of the MCO. Chemical scarification treatments with concentrated sulfuric acid proved to be efficient in overcoming seed tegumentary dormancy, since increasing imbibition occurred with times of 0, 8 and 72 hours. The seeds imbibition resulted in the primary root emission and integument disruption at the base of the seed, close to the hilum; the primary root showed a pivoting shape, light yellow in color and with few simple hairs. Soon after, from 48 to 72 hours, the tegument detachment occurred, leaving the fleshy cotyledons free, characterizing a phanerocotyledonary epigeal germination, with the control treatment exception. The hypocotyl is elongated and straight, with a light grayish-green coloration. The epicotyl is grayish-green yellow in color. The eophylls are composed, opposite, bipinnate, and have a dark yellow grayish-green color. The development of uniform seedlings, with the control treatment exception, which had little water absorption by the seeds. They are also evaluated using the biospeckle technique where the calculated descriptors are defined and mathematically formalized, by measuring the homogeneity or uniformity of the texture. This results in higher values when the intensities are uniform and the Inertia (MI) was more efficient in the analysis of high frequencies, which is due to its actuation mechanism, which is a second order moment, which favors variations in highest activity.

BANKING MEMBERS:
Interna - 2187545 - FLAVIA DE BARROS PRADO MOURA
Presidente - 1181648 - JOSE VIEIRA SILVA
Externo à Instituição - RUBENS PESSOA DE BARROS - UNEAL