Banca de QUALIFICAÇÃO: ANTONIO THOMÁS DA SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : ANTONIO THOMÁS DA SILVA
DATE: 12/04/2024
TIME: 08:30
LOCAL: Instituto de Ciências Farmacêuticas
TITLE:

Obtaining and Characterizing Coagulants from Milk Using Isolated Colonies of Enterococcus faecalis and Klebsiella variicola from the Intestine of the Adult Insect Rhynchophorus palmarum

KEY WORDS:

milk coagulants, characterization, proteases, Enterococcus faecalis and Klebsiella variicola

PAGES: 83
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Bioquímica dos Microorganismos
SUMMARY:

The food industry is one of the pillars of the Brazilian economy, with dairy products standing out as the third most relevant sector. In this context, enzymes play a crucial role in dairy production, especially in the coagulation of milk to make cheese. The search for alternatives to enzymes of animal origin has driven research into coagulants of microbial origin, highlighting the importance of studying these microorganisms for the production of enzymes of biotechnological interest in the food industry. In this sense, the present study isolated colonies of Enterococcus faecalis and Klebsiella variicola taken from the intestine of the adult insect Rhynchophorus palmarum to obtain milk coagulants. Enzymatic extracts of bacterial colonies were prepared in 50mM Tris-HCl buffer, pH 8.0, and pH, temperature, inhibition, effects of different substrates, electrophoresis, zymography and coagulation of skimmed milk powder were carried out. K. variicola and E. faecalis present differences in their biochemical parameters, with distinct activities and characteristics. While K.variicola shows activity in AZOCASEINE and requires an optimum pH of 8 and an optimum temperature of 50ºC, E. faecalis exhibits activity in AZOCASEINE and SAAPFpNA, with an optimum pH between 7, 8 and 9, and an optimum temperature of 50ºC. Regarding inhibitors, K. variicola is inhibited by EDTA and DTT, while E. faecalis is inhibited by PMSF. Both enzymes have an optimum temperature of 50ºC for coagulant activity, but they differ in the minimum protein concentration (0.5 µg for K. variicola and 10 µg for E. faecalis) and in the minimum calcium concentration (2mM for K. variicola and 8mM for E. faecalis) for coagulant activity. Therefore, the study concludes that it is possible to obtain milk coagulant from microorganisms from the microbiota of the adult insect R. palmarum, highlighting the viability of these alternative sources of coagulants in the dairy industry.

COMMITTEE MEMBERS:
Interno(a) - 1653558 - LUCIANO APARECIDO MEIRELES GRILLO
Externo(a) ao Programa - 1488396 - TICIANO GOMES DO NASCIMENTO - UFALExterno(a) à Instituição - JOHNNATAN DUARTE DE FREITAS - IFAL
Externo(a) à Instituição - JOSE MARCOS DOS SANTOS OLIVEIRA - CESMAC