Banca de QUALIFICAÇÃO: EMANOEL GUILHERMINO DA SILVA JUNIOR

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : EMANOEL GUILHERMINO DA SILVA JUNIOR
DATE: 16/01/2024
TIME: 15:30
LOCAL: Sala 205
TITLE:

SEASONALITY STUDY OF THE HYDROALCOHOLIC EXTRACT OF THE GREEN-BROWN PROPOLIS OF UNIÃO DOS PALMARES AND STANDARDIZATION OF CLAE-DAD TO QUANTIFY ITS PHENOLIC MARKERS

KEY WORDS:

Greenish-brown propolis from União dos Palmares; Seasonal variability; Biological activities.

PAGES: 120
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUBÁREA: Análise e Controle de Medicamentos
SUMMARY:

Propolis is a sticky, viscous natural substance with a characteristic odor. It has antiinflammatory, antiviral, antibacterial, antiparasitic, styptic, astringent, phytoinhibitory, phytotoxic, antioxidant, immunostimulating, hepatoprotective, cytotoxic, anticancer, antiallergic, antifungal, antiprotozoal, analgesic and antinociceptive activities. Brown propolis manifests highly variable chemical profiles throughout the Brazilian territory, which also happens, consequently, with its biological activity. Propolis has its chemical profile modified as a result of a synergism of environmental factors such as sunlight, temperature, humidity, precipitation, soil fertility and salinity. Therefore, the need for continuous improvement of the propolis quality control strategy stands out, with the aim of standardizing it. This study aimed to elucidate the seasonal variation of quality parameters of greenish-brown propolis (PMUP) from the Alagoas municipality of União dos Palmares-Alagoas-Brazil. HPLC-DAD confirmed pinocembrin as the most abundant bioactive compound in PMUP. Furthermore, artepillin-C, pinobanksin, pinostrobin, galangin and chrysin constituted markers whose concentrations were relatively high in the apiceutic period. The average content of total phenolic compounds, considering all months surveyed, consisted of 81.1 ± 26.05 mg EAG/g; the average total flavonoid content, 38.45 ± 10.10 mg EQ/g. Both results are above the concentration found in other types of brown propolis from Brazil. The average concentration of total flavanones and dihydroflavonols in PMUP was 1.59 ± 0.24 mg EP/20 mg and correspond to 7.95% of flavonones and flavononols. The average IC50 over the 12 months, in the DPPH• chemical test, was 47.72 ± 13.02 µg/mL, which can be classified as good, surpassing the sequestration capacity of other Brazilian brown propolis. The broth microdilution test revealed that Gram-positive bacteria were more sensitive to PMUP than Gram-negative bacteria. The month of January 2021, in which there was a peak in the concentration of total flavonoids, was the month in which PMUP showed the greatest capacity to inhibit the growth of all strains tested. The MICs obtained were as follows: 64-256 µg/mL for S. aureus; 256-1024 µg/mL for E. faecalis; 64-128 µg/mL for E. coli; and 64-256 µg/mL for C. albicans. In all chemical tests, bimodal variation was observed, which frequently comprised two four-month cycles with a high content of active metabolites and DPPH• radical scavenging capacity. PMUP proved to be a rich source of bioactive compounds, whose range of action is comprehensive. In vitro tests reveal the pharmacological potential of opototherapic, as its results remain significant even during periods of lower metabolite content.

COMMITTEE MEMBERS:
Interno(a) - 1885138 - ISABEL CRISTINA CELERINO DE MORAES PORTO
Externo(a) à Instituição - JOSE ROBERTO DE OLIVEIRA FERREIRA - UNCISAL
Externo(a) à Instituição - Salvana Priscylla Manso Costa - UFAL