Banca de QUALIFICAÇÃO: ALLYSSON FIRMINO DE FRANCA FARIAS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ALLYSSON FIRMINO DE FRANCA FARIAS
DATE: 05/02/2024
TIME: 14:00
LOCAL: ICF - Sala 205
TITLE:

PRE-CLINICAL TOXICOLOGICAL EVALUATION OF NANOENCAPSULATES LOADED WITH RED PROPOLIS EXTRACT FROM ALAGOAS USING ZEBRAFISH (DANIO RERIO) AS AN EXPERIMENTAL MODEL

KEY WORDS:

Red propolis, Zebrafish, Nanoparticles

PAGES: 90
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUBÁREA: Análise Toxicológica
SUMMARY:

Propolis is a resinous substance that has several pharmacological activities. However, most of the substances present have low bioavailability. In this context, nanoencapsulations represent a promising approach to optimize the bioavailability and stability of compounds. Therefore, this work aims to evaluate the pre-clinical toxicity of nanoencapsulated products loaded with red propolis extract from Alagoas. To this end, Zebrafish (Danio rerio) was used as an experimental model. The FET Test was used, following the guidelines established in OECD TG 236. The nanoencapsulated substances were diluted to the desired concentrations with E2 embryonic medium solution. 20 fertilized embryos were used for each concentration tested and for the negative control. The concentrations tested were 50 µg/mL; 25 µg/mL; 10 µg/ml; 2.5 µg/ml; and placebo. At the end of 96 hours, the animals were contained in a drop of 0.4% tricaine, on a microscope slide, in order to be photographed. The results showed that at higher concentrations (50 and 25 µg/mL) for all samples, there was a high mortality rate, reaching 100% of deaths, being evident mainly at 72 and 96 hpf. At lower concentrations of 2.5 and 10 µg/mL, the survival rate was higher, however sublethal and teratogenic effects were observed in zebrafish larvae. Ademias, it was found that the anomalies that appeared most were edemas in the pericardium and yolk sac, in addition to the absence of a non-inflated swim bladder, from 72 to 96 hpf. Therefore, it was possible to investigate the effects of exposing D. rerio embryos exposed to nanoencapsulated PCL: Pluronic; Eudragit: Pluronic; Liposomes, Inclusion Complexes and crude red propolis extract, in different concentrations: 0; 2.5; 10; 25 and 50 μg/mL, through the evaluation of lethal, sublethal and teratogenic effects, during periods of 24, 48, 72 and 96 hpf.

COMMITTEE MEMBERS:
Presidente - 1488396 - TICIANO GOMES DO NASCIMENTO
Interno(a) - 1885138 - ISABEL CRISTINA CELERINO DE MORAES PORTO
Interno(a) - 3509820 - MARIA ALINE BARROS FIDELIS DE MOURA
Externo(a) ao Programa - ***.813.564-** - JOSE ROBERTO DE OLIVEIRA FERREIRA - UNCISAL
Externo(a) ao Programa - ***.231.164-** - Salvana Priscylla Manso Costa - UFPE