Banca de QUALIFICAÇÃO: RAFAEL ALEIXO DOS SANTOS SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : RAFAEL ALEIXO DOS SANTOS SILVA
DATE: 30/08/2021
TIME: 14:00
LOCAL: Vídeoconferência
TITLE:

PHARMACOGNOSTIC EVALUATION AND ANTIFUNGAL ACTIVITY OF DRIED FRUITS FROM Illicium verum Hook. F. (SCHISANDRACEAE).

KEY WORDS:

Illicium verum; essential oil; quality control; dermatophytosis; cutaneous mycoses.

PAGES: 102
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUBÁREA: Farmacognosia
SUMMARY:

Illicium verum Hook f. (Schisandraceae), known as star anise, is a native specie to Asia marketed worldwide. The herbal drug (HD), consisting of dried fruit, is used in folk medicine and the pharmaceutical industry. In its essential oil is the anethole, the major component, to which pharmacological activities are mentioned. Cutaneous mycoses are clinical manifestations caused by a group of fungi called dermatophytes that affect humans and animals. Despite the various therapeutic options, the side effects and the resistance to antifungals are a problem for treatment, thus requiring the search for new antifungal agents. Despite its extensive use in phytotherapy and commercial, there are few studies dealing with pharmacognostic analysis of I. verum, like those of his activity on dermatophytes. Thus, the aim of this work was to make the quality control of dried fruits of I. verum and evaluate the antimycotic capacity of essential oil (IVEO) on dermatophytes. For the method, a sample of star anise was acquired in a herbal shop of the city of Maceió/AL and the quality control of HD was done according to the Brazilian Pharmacopoeia 6th edition (2019), being performed: botanical identification (macro- and microscopy), determination of foreign matter, total ash, yield and chemical profile of volatiles. Purity tests were performed in triplicate and expressed by mean and standard deviation (M±SD). For antifungal activity, six clinical samples of dermatophytes were taken: Microsporum canis (1), Trichophyton rubrum (1), Trichophyton tonsurans (1) and Trichophyton mentagrophytes (2), Trichophyton mentagrophytes var. interdigitale (1) and a sample of Candida albicans (ATCC 90028), additionally. The search consisted of microbiological screening using the agar diffusion method (pour plate technique) from 20 μL of pure IVEO (in natura) and by microdilution in broth, whose concentrations of IVEO were from 10,000 μg/mL to 78.1μg/mL and the results by diffusion in agar were expressed by the average of two measurements of the diameter of the halos (mm) and microdilution by the Minimum Inhibitory Concentration (MIC) and from this, that of the Minimum Fungicidal Concentration (MFC). The identity analysis showed that the samples met the macroscopic parameters of the HD. However, microscopy showed morphological differences in fruits that did not mature, indicating in them, absence of specialized structures for oil storage. The foreign matter yield (1.6%±0.32) and total ash (3.0%±0.10) were in accordance with the monograph of the specie. The percentage of the chemical marker anethole was in accordance with the monograph (>99%), however, the volatile yield was below the expected (6.25% w/v). To date, all microorganisms have been sensitive to IVEO by screening, with inhibition zones from 13.0 to 52.5 mm. The MIC/MFC for the samples were from 10,000 to 78.1μg/mL (MIC) and 10,000 to 625 ± μg/mL (MFC). The results of this search show that HD met the quality requirements proposed by the pharmacopoeia and ratifies the potential and activity of IVEO in fungi due to anethole.

BANKING MEMBERS:
Interno - 1553302 - IRINALDO DINIZ BASILIO JUNIOR
Externa à Instituição - MARIA ANILDA DOS SANTOS ARAÚJO - Tiradentes
Interno - 1488396 - TICIANO GOMES DO NASCIMENTO