Banca de QUALIFICAÇÃO: CAMILA BIANCA FERREIRA DA ROCHA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : CAMILA BIANCA FERREIRA DA ROCHA
DATE: 30/09/2021
TIME: 08:30
LOCAL: Vídeoconferência
TITLE:

Application and characterization of amylase produced by Tribolium castaneum larves in removal of dirt clothes

 

 

 

 

Application and characterization of amylase produced by Tribolium castaneum larves in removal of dirt clothes

A pest, in a broad sense, can be defined as the insect (or other organism) that causes direct damage to humans, their livestock, their crops, or even their possessions. The species Tribolium castaneum (Coleoptera: Tenebrionidae), stands out as one of the main pests, which, in particular, represents 9% of economic loss of stored grains in developed countries, so that, in developing countries, these losses can represent 20% worldwide. Due to its wide distribution around the globe and its characteristics, the intestine of this species can be used as a source of enzymes for studies for pest control and applications. The objective of this work was the characterization and biotechnological application of an amylase enzyme, from Tribolium castaneum larvae, in the removal of dirt from cotton fabrics. For this purpose, cotton fabrics, previously delimited and stained with superficial tomato sauce, were subjected to a washing process with enzymatic crude extract alone and in the presence of two commercial powder soaps, namely, ALA© and Tixan Ypê©. Furthermore, the crude extract was characterized in relation to parameters such as optimum temperature and thermal stability, optimum pH and behavior in the presence of some metallic ions, such as copper, zinc, calcium and the chelating agent EDTA. Spectrophotometry was used as an analytical method to prove the removal of dirt from the fabrics. It was observed that the amylase enzyme under study showed optimal relative activity over a wide pH range, namely, from 5 to 9, optimal temperature at 37°C and residual activity of more than 80% at 50°C, demonstrating, thus, thermal stability at this temperature. In the presence of some metallic ions, the highest inhibition rates, in relation to the 10mM, 1mM and 0.1mM concentrations, were related to copper sulfate (CuSO4), which comprised 95%, 89% and 86% inhibition, respectively. Regarding the detergency potential, it was observed that the enzymatic extract helped the cleaning power of the Tixan Ypê© soap powder by 48%, when compared only to the soap powder alone, and it aided in 88% in the cleaning power of the brand soap commercial ALA©, thus demonstrating a high efficiency of the crude extract, in the presence of these detergents, for the removal of dirt, thus implying an important ally and potential adjuvant enzyme to be incorporated in the powder soap industry and other products for the cleaning fabrics.

Amylase. Enzyme detergent. Tribolium castaneum.

 

KEY WORDS:

Amylase. Enzyme detergent. Tribolium castaneum.

PAGES: 63
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Enzimologia
SUMMARY:

A pest, in a broad sense, can be defined as the insect (or other organism) that causes direct damage to humans, their livestock, their crops, or even their possessions. The species Tribolium castaneum (Coleoptera: Tenebrionidae), stands out as one of the main pests, which, in particular, represents 9% of economic loss of stored grains in developed countries, so that, in developing countries, these losses can represent 20% worldwide. Due to its wide distribution around the globe and its characteristics, the intestine of this species can be used as a source of enzymes for studies for pest control and applications. The objective of this work was the characterization and biotechnological application of an amylase enzyme, from Tribolium castaneum larvae, in the removal of dirt from cotton fabrics. For this purpose, cotton fabrics, previously delimited and stained with superficial tomato sauce, were subjected to a washing process with enzymatic crude extract alone and in the presence of two commercial powder soaps, namely, ALA© and Tixan Ypê©. Furthermore, the crude extract was characterized in relation to parameters such as optimum temperature and thermal stability, optimum pH and behavior in the presence of some metallic ions, such as copper, zinc, calcium and the chelating agent EDTA. Spectrophotometry was used as an analytical method to prove the removal of dirt from the fabrics. It was observed that the amylase enzyme under study showed optimal relative activity over a wide pH range, namely, from 5 to 9, optimal temperature at 37°C and residual activity of more than 80% at 50°C, demonstrating, thus, thermal stability at this temperature. In the presence of some metallic ions, the highest inhibition rates, in relation to the 10mM, 1mM and 0.1mM concentrations, were related to copper sulfate (CuSO4), which comprised 95%, 89% and 86% inhibition, respectively. Regarding the detergency potential, it was observed that the enzymatic extract helped the cleaning power of the Tixan Ypê© soap powder by 48%, when compared only to the soap powder alone, and it aided in 88% in the cleaning power of the brand soap commercial ALA©, thus demonstrating a high efficiency of the crude extract, in the presence of these detergents, for the removal of dirt, thus implying an important ally and potential adjuvant enzyme to be incorporated in the powder soap industry and other products for the cleaning fabrics.

BANKING MEMBERS:
Interna - 1527220 - CAMILA BRAGA DORNELAS
Interno - 1653558 - LUCIANO APARECIDO MEIRELES GRILLO
Externo ao Programa - 2089586 - FRANCIS SOARES GOMES