Banca de QUALIFICAÇÃO: ELANE CONCEICAO DOS SANTOS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ELANE CONCEICAO DOS SANTOS
DATE: 18/02/2022
TIME: 08:30
LOCAL: Vìdeoconferência
TITLE:

IMMUNOMODULATORY ACTIVITY OF ALAGOAS RED PROPOLIS IN MURINE MACROPHAGES ACTIVATED BY LIPOPOLYSACCHARIDE IN VITRO

 

KEY WORDS:

Red propolis, macrophage, immunomodulation, isoliquiritigenin, formononetin. 

PAGES: 67
BIG AREA: Ciências Biológicas
AREA: Imunologia
SUBÁREA: Imunologia Aplicada
SUMMARY:

The Alagoas red propolis (PVA) is a natural red resin, produced by Apis mellifera bees from plant exudate (Dalbergia ecastophyllum), which has several biological properties, including anti-inflammatory activity. Inflammation is the body's response mechanism against various injuries; however, this exacerbated process can trigger an inflammatory disease. Thus, the search for new natural immunomodulatory agents is necessary for the development of new therapeutic approaches that modulate the inflammatory process. In this context, the objective of this work was to evaluate the immunomodulatory potential of PVA and its constituents isoliquiritigenin (ISL) and formononetin (FMT) in murine macrophages stimulated with lipopolysaccharide (LPS) in vitro. Initially, the maximum non-toxic concentration (CMNT) of the hydroalcoholic extract (EH) of PVA, ISL and FMT in murine macrophages of the J774A.1 strain was determined, based on the analysis of cell viability by the MTT assay. Then, the EH immunomodulatory activities of PVA, ISL or FMT were evaluated by treating LPS-stimulated macrophages under different experimental conditions. The expression of markers F4/80 and CD86 was evaluated by multiparametric flow cytometry and the quantification of pro and anti-inflammatory cytokines was evaluated by the Cytometeric Bead Array (CBA) methodology in the cell culture supernatant. Activation of MAPKs (mitogen-activated protein kinases) was evaluated by analyzing the phosphorylation of ERK1/2, p38 and JNK proteins by intracellular flow cytometry. The expression of NOS2, Arg1, SOCS3, TGF-β, Lamp2, MIP-1α, Ccne1 genes was evaluated by RT-qPCR. It was observed that treatment with EH of PVA reduced the percentage of double positive cells (F4/80+ CD86+), decreased the expression of the activating molecule CD86 and the levels of pro-inflammatory cytokines IL-6 and TNF. Furthermore, an increase in ERK1/2, p38 and JNK phosphorylation and a decrease of NOS2 and SOCS3 expression were detected 48h after treatment. The treatment with ISL significantly reduced the frequency of F4/80+ CD86+ cells, decreased CD86 expression and phosphorylation of ERK1/2 and p38 proteins. On the other hand, FMT treatment did not reduce the percentage of double positive cells (F4/80+ CD86+) in LPS-stimulated murine macrophages. In conclusion, in this study, it was detected a promising immunomodulatory activity of PVA and the ISL compound on murine macrophages in vitro. These results contribute to a better understanding of the immunomodulatory activity of PVA and its constituents, favoring the development of new therapies and immunological interventions for the treatment of inflammatory diseases. 

BANKING MEMBERS:
Interno - 1612086 - ENIO JOSE BASSI
Interno - 1488396 - TICIANO GOMES DO NASCIMENTO
Externo à Instituição - Vinicius de Andrade Oliveira