Banca de DEFESA: CAMILA BIANCA FERREIRA DA ROCHA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CAMILA BIANCA FERREIRA DA ROCHA
DATE: 08/03/2022
TIME: 08:00
LOCAL: Vìdeoconferência
TITLE:

CHARACTERIZATION OF AMYLASE PRODUCED BY TRIBOLIUM CASTANEUM LARVAE AND APPLICATION IN THE REMOVAL OF DIRT FROM COTTON FABRICS

 

 

 

KEY WORDS:

Palavras chaves em inglês Carbohydratease. Enzyme detergent. Red flour beetle. Enzymes.

PAGES: 62
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Enzimologia
SUMMARY:

A pest, in a broad sense, can be defined as the insect (or other organism) that causes direct damage to humans, their livestock, their crops, or even their possessions. The species Tribolium castaneum Herbst (Coleoptera: Tenebrionidae), stands out as one of the main pests, which, in particular, represents 9% of economic loss of stored grains in developed countries, so that, in developing countries, these losses can represent 20% worldwide. Due to its wide distribution around the globe and its characteristics, the intestine of this species can be used as a source of enzymes for studies for pest control and applications. The objective of this work was the characterization and biotechnological application of an amylase enzyme, from Tribolium castaneum larvae, in the removal of dirt from cotton fabrics. For this purpose, cotton fabrics, previously delimited and stained with starch solution at 1% commercial tomato sauce, were subjected to a washing process with enzymatic crude extract alone and in the presence of two commercial powder soaps, detergent A and detergent B. Furthermore, the crude extract was characterized in relation to parameters such as optimum temperature and thermal stability, optimum pH and behavior in the presence of some metallic ions, such as copper, zinc, calcium and the chelating agent EDTA. Spectrophotometry was used as an analytical method to prove the removal of dirt from the fabrics. It was observed that the amylase enzyme under study showed optimal relative activity over a wide pH range, namely, from 5 to 9, optimal temperature at 37°C and residual activity of more than 80% at 50°C, demonstrating, thus, thermal stability at this temperature. In the presence of some metallic ions, the highest inhibition rates, in relation to the 10mM, 1mM and 0.1mM concentrations, were related to copper sulfate (CuSO4), which comprised 95%, 89% and 86% inhibition, respectively. In relation to the potentiality of detergency, it was observed that the enzymatic extract had the ability to break starch in dirty tissues with starch solution and also demonstrated superior cleaning power, when compared to comercial detergents under study, in front of the dirty tissue commercial tomato sauce thus implying an importante ally and potential adjuvant enzyme to be incorporated in the industry of enzymatic detergents and other products for cleaning.

BANKING MEMBERS:
Interna - 1527220 - CAMILA BRAGA DORNELAS
Interno - 1653558 - LUCIANO APARECIDO MEIRELES GRILLO
Externo ao Programa - 2089586 - FRANCIS SOARES GOMES