Banca de QUALIFICAÇÃO: VIVIANE AMARAL PORTO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : VIVIANE AMARAL PORTO
DATE: 02/03/2023
TIME: 14:00
LOCAL: Vìdeoconferência
TITLE:

Evaluation of sodium methylarsinate toxicity in zebrafish embryos (Danio rerio) through toxicological study and NMR-based metabolomics

KEY WORDS:

arsenic, ecotoxicology, biomarkers, metabonomics

PAGES: 92
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Metabolismo e Bioenergética
SUMMARY:

Pesticides and herbicides are intended to improve crop productivity, protecting them and preventing the proliferation of pests. Equally, the use of veterinary products is based on the search for higher yields in livestock. In this context, substances derived from the element arsenic (As), such as sodium methylarsinate (MSMA), are present in several products for veterinary and agricultural use. Considering that such products are widely used in Brazil, and other countries, the topics that clarify the risks and toxicity of these compounds have not yet been fully explored. Metabolomics is a branch of the omics sciences that seeks to understand, through the analysis of the metabolites produced in a given organism, which factors influence specific metabolic changes, whether these occur due to lifestyle habits or exposure to any xenobiotic. The metabolomics strategy includes the analysis of biological samples using equipment such as Gas Chromatography (GC) and Nuclear Magnetic Resonance (NMR). Thus, this project aimed to investigate, through an ecotoxicological study associated with metabolomics based on 1H NMR, the proportion of impact that arsenic compounds in water can provoke on aquatic species such as zebrafish (Danio rerio). The choice of this species as a model organism was due to its considerable versatility in bioassays. The toxicity study was based on the acute exposure of embryos to MSMA, with serial concentrations in order to obtain the LD50. Sampling for the metabolomic study followed the protocols available in the literature, associated with 1H NMR spectroscopy and statistical study to get the metabolic profile. Thus, the metabolomic analysis revealed 42 metabolites involved in different metabolic pathways. The biochemical profile study suggested that MSMA caused severe impacts, especially on pathways of energy metabolism, endocrine metabolism, hepatic metabolism, and oxidative stress.

BANKING MEMBERS:
Interno(a) - 1653558 - LUCIANO APARECIDO MEIRELES GRILLO
Externo(a) à Instituição - RICARDO OLIVEIRA DA SILVA - UFPE
Externo(a) à Instituição - THIAGO LOPES ROCHA - UFG
Interno(a) - 1366289 - THIAGO MENDONCA DE AQUINO