Banca de DEFESA: MARIA LUIZA DAL PONT MACHADO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARIA LUIZA DAL PONT MACHADO
DATE: 27/03/2023
TIME: 14:00
LOCAL: Instituto de Ciências Farmacêuticas
TITLE:

Hepatoprotective effect of alpha-terpineol in rats with isoproterenol-induced metabolic syndrome

KEY WORDS:

Monoterpene ; Hepatoprotection; Isoproterenol; Rats.

PAGES: 86
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Introduction: The α- terpineol (TPN) is a monoterpene obtained from Protium hepthaphylum. That has several pharmacological activities such as: anti-inflammatory, anti-obesity and cardioprotection on Spontaneously Hypertensive Rats (SHR) by isoproterenol model. ISO evoked metabolic disturbance on hepatocytes, stimulate fatty acids storage on vesicles inducing non-alcoholic steatosis. The aims of this study were verify the hepatoprotective effects of TPN in SHR against liver damage induced by ISO. Methods: Experimental protocols were approved by the CEUA/UFAL nº09/2015. Male rats were allocated into 5 groups and treated for 15 days (n=5): (G1= saline 0.9% P.O/d); (G2= Infarcted saline 0.9% P.O./d + ISO 85mg/kg 2x s.c); (G3= TPN 25 mg/kg P.O./d); (G4=TPN 50 mg/kg P.O./d) and (G5=TPN 75 mg/kg P.O./d). On 16º day, rats were anesthetized (Ketamine 80 mg/kg + Xylazine 4 mg/kg i.p.) and morphometric tests were performed in accordance with Scherle´s methods. Morphology tests were examined under light by stereoscopy, while lipid levels were estimated by total cholesterol and triglyceride dosages of blood serum. The results were expressed as mean ± SEM, and analyzed statistically by ANOVA one-way followed by Newman- Keuls test considered significant when * p <0.05, ** p<0.001 and *** p< 0.0001. Results: Weight of the liver (G1= 6.92 ± 0.22g/g* vs. G2= 8.67 ± 0.3g/g), (G4= 6.40 ± 0.36g/g* vs. G2= 8.67 ± 0.31g/g), (G5= 5.22 ± 0.7g/g*** vs. G2= 8.67 ± 0.31g/g) and (G3= 7.44 ± 0.6g/g vs. G5= 5.22 ± 0.7g/g), reason liver weight by body weight (G3=1.86 ± 0.05g/g* vs. G4= 2.44 ± 0.16g/g), (G3=1.86 ± 0.05g/g** vs G5= 2.64 ± 0.23g/g), (G4= 2.44 ± 0.16g/g *vs. G2= 1.68 ± 0.05g/g) and (G5= 2.64 ± 0.23g/g** vs. G2= 1.68 ± 0.05g/g) and reason liver weight by femur weight (G1= 65.7± 9.24g/g**** vs. G2= 547.4 ± 7.15g/g), (G3= 834.4 ± 9.51g/g**** vs. G2= 547.4 ± 7.15g/g Through morphological and histopathological aspects, it was observed that TPN inhibited non-alcoholic hepatic steatosis due to damage caused by induction with isoproterenol, in addition, previous treatment with TPN significantly inhibited the increase in total cholesterol levels (G1= 21,5 ± 1,0 vs. G2= 68,1 ± 1,2****), (G2= 68,1 ± 1,2****vs. G3 = 20,7 ± 1,3****), (G2= 68,1 ± 1,2**** vs. G4 = 22,6± 1,5****) and (G2= 68,1 ± 1,2****vs G5= 22,3 ± 4,6 ****) and triglyceride G1= 23,4 ± 1,6 vs. G2= 353,4 ± 23,3 ****), (G2= 353,4 ± 23,3 vs. G3 = 28,9 ± 2,8****), (G2= 353,4 ± 23,3 vs. G4 = 27,5± 1,7****) and (G2= 353,4 ± 23,3 vs G5= 16,2 ± 1,5 ****), levels at all doses tested. In addition, after the glucose tolerance curve, it was noted that there was a reversal in this curve, which may also influence glucose metabolism, however further studies are needed. Conclusion: Taken together, the results indicate that TPN has a significant hepatoprotective effect, in hypertensive and infarcted rats.

BANKING MEMBERS:
Presidente - 1547952 - EURICA ADELIA NOGUEIRA RIBEIRO
Interno(a) - 1488396 - TICIANO GOMES DO NASCIMENTO
Externo(a) ao Programa - 1063641 - CAMILLA CAMERINO SANTANA DAVINO FREIRE