Banca de DEFESA: JOAO VICTOR LESSA DE OLIVEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JOAO VICTOR LESSA DE OLIVEIRA
DATE: 22/03/2023
TIME: 13:30
LOCAL: Sala 205 do PPGCF
TITLE:

INVESTIGATION OF THE CHEMICAL COMPOSITION AND EVALUATION OF THE ANTIBACTERIAL POTENTIAL OF BROWN PROPOLIS EXTRACT FROM THE REGION OF UNIÃO DOS PALMARES, ALAGOAS

KEY WORDS:

Brown Propolis. Chemical composition. Flavonoids. Flavones. Antibacterial activity. Antioxidant activity.zv

PAGES: 91
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

The brown and red type propolis from the mangrove region of Alagoas are evidenced in Alagoas, being the red propolis, of defined botanical origin. The study of the chemical composition of brown propolis from the region of União dos Palmares has been the subject of this study. The study of the chemical composition is being carried out through different analytical techniques and analytical methods such as fingerprint studies (fingerprint) and possible botanical origin using HPLC-DAD-UV, LC-Orbitrap-FTMS techniques, chemical assays of total flavonoids and activity antioxidant, as well as microbiological assay of Minimum Inhibitory Concentration. The research consisted of collecting brown propolis from the municipality of União dos Palmares (PMUP) for extraction from September 2019 to August 2021. An amount between 38 and 91g of in natura sample was collected from apiaries close to the municipality of União dos Palmares. The extraction method chosen for PMUP was maceration, in two cycles of 48 hours, using 15 g of PMUP and, for each cycle, 50 ml of hydroethanolic solution at 92.8 ºGL. The extracts were pooled and concentrated to obtain a crude solvent-free extract. Crude extracts 5 (January/2021) and 12 (July/2021) were stored in a freezer until the moment of total flavonoids, antioxidant activity by DPPH, microbiological assays and chromatographic profile assay. A brown propolis key marker isolation study was performed with a brown propolis sample from October 2019 that was subjected to partitioning using liquid-liquid extraction to obtain hexane, hexane-ethyl acetate (9:1), hexane-ethyl acetate (7 :3) and dichloromethane fraction. The dichloromethane phase was subjected to column chromatography on cellulose as a solid support and Methanol:H2O acidified with acetic acid (1%) to obtain fractions between 0% methanol and 100% methanol. The MeOH10%, MeOH20%, MeOH30% and MeOH45% fractions were submitted to semi-preparative HPLC-UV for the isolation of 2 main markers (pk35; pinocembrin) and (pk23; naringenin in mixture with naringenin) of brown propolis (PMUP) that were identified by LC-Orbitrap-FTMS and NMR 1H and 13C. The phenolic compounds identified by the LC-MS technique based on comparison with data from the literature were divided into benzoic acids, cinnamic acids (caffeic acid, ferulic acid), chlorogenic acid, prenylated cinnamic acids (dupranin and artepillin C), stilbene (resveratrol), coumarins and flavonoids divided into flavanonols, flavonones, flavones, flavonols, flavana-3-ol, flavana-4-ol and chalcones. Crude PMUP extracts showed total flavonoid content of 0.5 to 2% based on the quercetin pattern and high antioxidant activity with DPPH free radical scavenging percentage between 80 and 90%. The microbiological assay using Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Candida albicans, Trichophyton mentagrophytes and Microsporum canis strains showed MIC between 32 and 1024 µg/mL for MEOH20% fraction and MIC between 16 and 256 µg/mL for MEOH35% fraction.

 

BANKING MEMBERS:
Interno(a) - 1547952 - EURICA ADELIA NOGUEIRA RIBEIRO
Interno(a) - 1488396 - TICIANO GOMES DO NASCIMENTO
Externo(a) ao Programa - 3182288 - VANDERSON BARBOSA BERNARDO